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Protein

Single-minded homolog 1

Gene

Sim1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. protein heterodimerization activity Source: UniProtKB
  3. sequence-specific DNA binding transcription factor activity Source: InterPro
  4. signal transducer activity Source: InterPro

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. nervous system development Source: UniProtKB-KW
  3. regulation of transcription, DNA-templated Source: MGI
  4. transcription, DNA-templated Source: UniProtKB-KW
  5. ureteric bud development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Single-minded homolog 1
Short name:
mSIM1
Gene namesi
Name:Sim1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:98306. Sim1.

Subcellular locationi

  1. Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 765765Single-minded homolog 1PRO_0000127440Add
BLAST

Proteomic databases

PRIDEiQ61045.

PTM databases

PhosphoSiteiQ61045.

Expressioni

Tissue specificityi

Detected in lung, skeletal muscle and kidney. During fetal development it is found in the CNS, developing kidney, mesodermal and endodermal tissues, including developing somites, mesonephric duct, and foregut.

Gene expression databases

BgeeiQ61045.
CleanExiMM_SIM1.
GenevestigatoriQ61045.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Heterodimer of SIM1 and ARNT.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ArntP537622EBI-78890,EBI-78852

Protein-protein interaction databases

IntActiQ61045. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ61045.
SMRiQ61045. Positions 2-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5353bHLHPROSITE-ProRule annotationAdd
BLAST
Domaini77 – 14771PAS 1PROSITE-ProRule annotationAdd
BLAST
Domaini218 – 28871PAS 2PROSITE-ProRule annotationAdd
BLAST
Domaini292 – 33544PACAdd
BLAST
Domaini336 – 765430Single-minded C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi368 – 38720Nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation
Contains 1 Single-minded C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG310284.
GeneTreeiENSGT00760000118788.
HOGENOMiHOG000236298.
HOVERGENiHBG003936.
InParanoidiQ61045.
KOiK09100.
OMAiGEVCHSS.
OrthoDBiEOG790G0R.
PhylomeDBiQ61045.
TreeFamiTF317772.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR013655. PAS_fold_3.
IPR010578. SIM_C.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
PF00989. PAS. 1 hit.
PF08447. PAS_3. 1 hit.
PF06621. SIM_C. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
PS51302. SIM_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEKSKNAAR TRREKENSEF YELAKLLPLP SAITSQLDKA SIIRLTTSYL
60 70 80 90 100
KMRVVFPEGL GEAWGHTSRT SPLDNVGREL GSHLLQTLDG FIFVVAPDGK
110 120 130 140 150
IMYISETASV HLGLSQVELT GNSIYEYIHP ADHDEMTAVL TAHQPYHSHF
160 170 180 190 200
VQEYEIERSF FLRMKCVLAK RNAGLTCGGY KVIHCSGYLK IRQYSLDMSP
210 220 230 240 250
FDGCYQNVGL VAVGHSLPPS AVTEIKLHSN MFMFRASLDM KLIFLDSRVA
260 270 280 290 300
ELTGYEPQDL IEKTLYHHVH GCDTFHLRCA HHLLLVKGQV TTKYYRFLAK
310 320 330 340 350
QGGWVWVQSY ATIVHNSRSS RPHCIVSVNY VLTDTEYKGL QLSLDQISAS
360 370 380 390 400
KPTFSYTSSS TPTISDNRKG AKSRLSSSKS KSRTSPYPQY SGFHTERSES
410 420 430 440 450
DHDSQWGGSP LTDTASPQLL DPERPGSQHE LSCAYRQFPD RSSLCYGFAL
460 470 480 490 500
DHSRLVEDRH FHTQACEGGR CEAGRYFLGA PPTGRDPWWG SRAALPLTKA
510 520 530 540 550
SPESREAYEN SMPHITSIHR IHGRGHWDED SVVSSPDPGS ASESGDRYRT
560 570 580 590 600
EQYQNSPHEP SKIETLIRAT QQMIKEEENR LQLRKAPPDQ LASINGAGKK
610 620 630 640 650
HSLCFANYQQ PPPTGEVCHS SALASTSPCD HIQQREGKML SPHENDYDNS
660 670 680 690 700
PTALSRISSP SSDRITKSSL ILAKDYLHSD MSPHQTAGDH PAISPNCFGS
710 720 730 740 750
HRQYFDKHAY TLTGYALEHL YDSETIRNYS LGCNGSHFDV TSHLRMQPDP
760
AQGHKGTSVI ITNGS
Length:765
Mass (Da):85,541
Last modified:July 15, 1999 - v3
Checksum:iB1A7F7DA8578CD17
GO

Sequence cautioni

The sequence AAA91201.1 differs from that shown. Reason: Erroneous termination at position 697. Translated as Cys.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti133 – 1331H → L in AAA91201 (PubMed:8812055).Curated
Sequence conflicti133 – 1331H → L in AAC05481 (PubMed:8812055).Curated
Sequence conflicti176 – 1761Missing in AAA91201 (PubMed:8812055).Curated
Sequence conflicti322 – 3221P → R in AAA91201 (PubMed:8812055).Curated
Sequence conflicti322 – 3221P → R in AAC05481 (PubMed:8812055).Curated
Sequence conflicti480 – 4801A → P in AAA91201 (PubMed:8812055).Curated
Sequence conflicti537 – 5371D → S in AAA91201 (PubMed:8812055).Curated
Sequence conflicti698 – 6981F → K in AAA91201 (PubMed:8812055).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40575 mRNA. Translation: AAA91201.1. Sequence problems.
AF038857
, AF038853, AF038854, AF038856, AF044913, AF038855 Genomic DNA. Translation: AAC05481.1.
D79209 mRNA. Translation: BAA11467.1.
AB013491 Genomic DNA. Translation: BAA28270.1.
CCDSiCCDS48556.1.
RefSeqiNP_035506.2. NM_011376.3.
XP_006512690.1. XM_006512627.2.
UniGeneiMm.4774.

Genome annotation databases

EnsembliENSMUST00000020071; ENSMUSP00000020071; ENSMUSG00000019913.
GeneIDi20464.
KEGGimmu:20464.
UCSCiuc007fal.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40575 mRNA. Translation: AAA91201.1. Sequence problems.
AF038857
, AF038853, AF038854, AF038856, AF044913, AF038855 Genomic DNA. Translation: AAC05481.1.
D79209 mRNA. Translation: BAA11467.1.
AB013491 Genomic DNA. Translation: BAA28270.1.
CCDSiCCDS48556.1.
RefSeqiNP_035506.2. NM_011376.3.
XP_006512690.1. XM_006512627.2.
UniGeneiMm.4774.

3D structure databases

ProteinModelPortaliQ61045.
SMRiQ61045. Positions 2-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61045. 3 interactions.

PTM databases

PhosphoSiteiQ61045.

Proteomic databases

PRIDEiQ61045.

Protocols and materials databases

DNASUi20464.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020071; ENSMUSP00000020071; ENSMUSG00000019913.
GeneIDi20464.
KEGGimmu:20464.
UCSCiuc007fal.1. mouse.

Organism-specific databases

CTDi6492.
MGIiMGI:98306. Sim1.

Phylogenomic databases

eggNOGiNOG310284.
GeneTreeiENSGT00760000118788.
HOGENOMiHOG000236298.
HOVERGENiHBG003936.
InParanoidiQ61045.
KOiK09100.
OMAiGEVCHSS.
OrthoDBiEOG790G0R.
PhylomeDBiQ61045.
TreeFamiTF317772.

Miscellaneous databases

NextBioi298559.
PROiQ61045.
SOURCEiSearch...

Gene expression databases

BgeeiQ61045.
CleanExiMM_SIM1.
GenevestigatoriQ61045.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR013655. PAS_fold_3.
IPR010578. SIM_C.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
PF00989. PAS. 1 hit.
PF08447. PAS_3. 1 hit.
PF06621. SIM_C. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
PS51302. SIM_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression patterns of two murine homologs of Drosophila single-minded suggest possible roles in embryonic patterning and in the pathogenesis of Down syndrome."
    Fan C.-M., Kuwana E., Bulfone A., Fletcher C.F., Copeland N.G., Jenkins N.A., Crews S., Martinez S., Puelles L., Rubenstein J.L., Tessier-Lavigne M.
    Mol. Cell. Neurosci. 7:1-16(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/Sv and Swiss Webster.
    Tissue: Embryonic brain and Embryonic stem cell.
  2. "Cloning of two human homologs of the Drosophila single-minded gene SIM1 on chromosome 6q and SIM2 on 21q within the Down syndrome chromosomal region."
    Chrast R., Scott H.S., Chen H., Kudoh J., Rossier C., Minoshima S., Wang Y., Shimizu N., Antonarakis S.E.
    Genome Res. 7:615-624(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  3. "Two new members of the murine Sim gene family are transcriptional repressors and show different expression patterns during mouse embryogenesis."
    Ema M., Morita M., Ikawa S., Tanaka M., Matsuda Y., Gotoh O., Saijoh Y., Fujii H., Hamada H., Kikuchi Y., Fujii-Kuriyama Y.
    Mol. Cell. Biol. 16:5865-5875(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  4. "Mouse single-minded1 (mSim1) gene."
    Hosoya T.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  5. "Two murine homologs of the Drosophila single-minded protein that interact with the mouse aryl hydrocarbon receptor nuclear translocator protein."
    Probst M.R., Fan C.-M., Tessier-Lavigne M., Hankinson O.
    J. Biol. Chem. 272:4451-4457(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiSIM1_MOUSE
AccessioniPrimary (citable) accession number: Q61045
Secondary accession number(s): O70284, P70183
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1999
Last modified: April 1, 2015
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.