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Q61043

- NIN_MOUSE

UniProt

Q61043 - NIN_MOUSE

Protein

Ninein

Gene

Nin

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppressed release of microtubules.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi245 – 2528GTPSequence Analysis
    Nucleotide bindingi300 – 3045GTPSequence Analysis
    Nucleotide bindingi420 – 4234GTPSequence Analysis

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. kinase binding Source: MGI
    4. protein binding Source: MGI

    GO - Biological processi

    1. centrosome-templated microtubule nucleation Source: MGI
    2. microtubule anchoring at centrosome Source: MGI

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ninein
    Gene namesi
    Name:Nin
    Synonyms:Kiaa1565
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:105108. Nin.

    Subcellular locationi

    Cytoplasmcytoskeletonmicrotubule organizing centercentrosome 1 Publication
    Note: Component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization.

    GO - Cellular componenti

    1. centriole Source: MGI
    2. centrosome Source: HGNC
    3. microtubule Source: UniProtKB-KW
    4. spindle pole Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 20352035NineinPRO_0000096845Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1540 – 15401PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/Aurora kinase B.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ61043.

    PTM databases

    PhosphoSiteiQ61043.

    Expressioni

    Tissue specificityi

    Widely expressed. Highly expressed in spleen, bone marrow and skin. Weakly expressed in liver and small intestine. Expressed in brain.1 Publication

    Developmental stagei

    Associated with the centrosome throughout the cell cycle. During mitosis, it is associated with the centrosome and the mitotic spindle. At anaphase, it is only localized to centrosomes.1 Publication

    Gene expression databases

    ArrayExpressiQ61043.
    BgeeiQ61043.
    CleanExiMM_NIN.
    GenevestigatoriQ61043.

    Interactioni

    Subunit structurei

    Homooligomer. Interacts with GSK3B/GSK3-beta via its C-terminal domain. Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3B By similarity. Interacts with AUNIP (via N-terminus) By similarity. Identified in a complex with AUNIP and AURKA By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201772. 2 interactions.
    IntActiQ61043. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61043.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 4336EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini42 – 7736EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini182 – 21736EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini219 – 25234EF-hand 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini317 – 35236EF-hand 5PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili357 – 1021665Sequence AnalysisAdd
    BLAST
    Coiled coili1064 – 109431Sequence AnalysisAdd
    BLAST
    Coiled coili1178 – 1328151Sequence AnalysisAdd
    BLAST
    Coiled coili1424 – 2013590Sequence AnalysisAdd
    BLAST

    Domaini

    The coiled coil region from Asn-1601 to Pro-1682 is necessary and sufficient for the targeting to centrosome.

    Sequence similaritiesi

    Contains 5 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiNOG124644.
    GeneTreeiENSGT00660000095541.
    HOGENOMiHOG000293170.
    HOVERGENiHBG052623.
    KOiK16476.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PROSITEiPS50222. EF_HAND_2. 5 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q61043-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDEVEEDQHE ARLKELFDSF DTLGTGSLGQ EELTDLCHVL CLEDVGPVLQ     50
    QTLLQDNLLG RVHFDQFKEA LILILSRTLS SEEHFEESDC SPEAQPKYVR 100
    GGKRYGRRSL PEFQESGEEI EEVTVLEPLE EEARSSPIPA GDCGEHWKTQ 150
    RSEEYEAEGQ LRFWNPDDLN ASHGGSCPPP DWIEEKLQEV CEDLGITRDG 200
    HLNRKKLVSI CEQYGLQNVD GAMLEEVFLS LDPDGTMSVE DFFYGLFKTG 250
    KSLTPSASTP YRQLKRHLSM QSFDESGRRT ATSSAMTSTI GFRVFSCLDD 300
    GMGQASVERI LDTWQEEGIE NSQEILKALD FSLDGNINLT ELTLALENEL 350
    LVTKNGIHQA ALASFKAEIR HLLERVDQVV REKEKLRSDL DKAEKLKSLM 400
    ASEVDDHHAA IERRNEYNLR KLDEEYKERI AALKNELRQE REQMLQQVGK 450
    QRVELEQEIQ KAKTEENYIR DRLALSLKEN NRLETELLEN AEKLAEYESL 500
    TQKLQRSLEN VLAEKFGDLD PSSAEFFLQE ERLAQMRNEY EQQCRLLQDQ 550
    VDELQSELEE YQAQGRVLRL PLKNSLSEEL DGHSGGIEPD QGPGSEECNP 600
    LNMSIEAELV IEQMKEQHHR DLCHLRLELE DKVRHYEKQL DDTRVASEQE 650
    QAAMKQKYEQ GVHTLEKRVS ELRSEIADLE GQAAVLREAH HKASCRHEEE 700
    KRQLQMAFDE EKAQLQEELR QEHERELQAR LQQAAESFRQ EREGLAQAAW 750
    TEEKVRGLEQ SYQEQLLSLE EKHALEKEEL REELSEHHRR ELQEGREEME 800
    TECNRRVSQI EAQCQADCEK VTEHCEQTLQ SLEVRHRQEL RDLLDQHLEE 850
    RSQWEFEKDE LTQECTDAQE QLKEALQRER ATAAAMKQEQ EILERTYKDR 900
    LNILSTERKQ LLQDLKDLQN ASESQHGLLS GQILELKRSQ ERELRDQGQA 950
    LCQTGVSEQL ASQQLERLRV EHEQERREMT GKLAALESAH RASLERADQE 1000
    KAEMSTEICR LQNTVKDMQQ AASLLMLQGG CQATAGEEAE GDGAMSLLQQ 1050
    GEQLLEENGD VLISLQRAHE HAVKENAKMA TEISRLQQRL KKLEPGSVIS 1100
    SCLEEGTSEI SGSSREQVEP IMKQGPATKH FLSDLGDHEA RDLASTGTSS 1150
    VQRQECKTEA SEASLDCFSE LENSEDTRTE SWDLKSQISQ LREQLTVLRA 1200
    DCDRASERKQ DLLFDISVLK KKLKMLERLP EASSRYKVLY EDAARENSCL 1250
    QEELRLVETR YEESLDSNKE LTAEVYRLQD EMKKMEEVME TFLSLEKSYD 1300
    EVKVENEELR ALVLRLQGKM EKVLGRAALQ GDSYALWEAP SENLEVASDE 1350
    KMLELRQTPK ECTPKVVSMH HIIEECTQET QCCEQGSTKL LARIKAHEIA 1400
    WFHRAIKTHP EKPSAQNRVI PEGSAALLGL QDKHLQQEAT IAELELEKQK 1450
    LQELTRNLRE RVTALVRQKD APSQGQKEEE LKAMMQDLQI TCGEMQRKVE 1500
    LLRYESEKLQ EENSILRNEI TTLNEEDSIS NLKLEELNGS QEELWQKIET 1550
    IEQEKASIQT MVEKLKKQVS DLKIKNQQLD SENIELSQKN SQNKEELKTL 1600
    NQRLAEMLCQ REEPGACTSE KWEQENASLK EELDHYKVQT STLVSSLEAE 1650
    LSEVKLQTHV MEQENLLLKD ELERLKQLHR CPDLSDFQQK MSSILSYNEK 1700
    LLKEKEVLSE ELKSCADKLA ESSLLEHRIA TMKQEQTAWE EQSESLKSQL 1750
    AVSQAKVQNL EDVLQNVNLQ MAEIESDLQV TRQEKEALKQ EVMSLHRQLQ 1800
    NAIDKDWVSE TAPHLSGLRG QQRRLSWDKL DHLMNEEPQL LCQESKRLQT 1850
    VVQNTQADLT HSREKVRQLE SNLLPTKHQK QLNQPCTVKS TEQEKLTLKR 1900
    ECEQSQKEQS PTSRKVGQMG SLERGLETIH LENEGLKKKQ VRLDEKLMEM 1950
    QPLRSTVTRS PSSHWDLQLL QQQACPMVPR EQFLQLQQQL LQAEKRSQHL 2000
    QEELENRTSE TNTPQALLLE QRAVHADSCR RIGHL 2035
    Length:2,035
    Mass (Da):234,723
    Last modified:July 27, 2011 - v3
    Checksum:iF92C1E77C1365D19
    GO
    Isoform 2 (identifier: Q61043-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1941-1949: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:2,026
    Mass (Da):233,608
    Checksum:iE7E37BD4108D2560
    GO

    Sequence cautioni

    The sequence AAA83234.1 differs from that shown. Reason: Frameshift at position 2029.
    The sequence BAC98204.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti145 – 1462EH → DD in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti341 – 3422EL → DV in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti383 – 39412KEKLR…LDKAE → RRAYGRTWTSR in AAA83234. (PubMed:8834802)CuratedAdd
    BLAST
    Sequence conflicti506 – 5061R → A in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti561 – 5611Y → H in BAC98204. (PubMed:14621295)Curated
    Sequence conflicti737 – 7371S → T in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti862 – 8621T → A in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti909 – 9091K → E in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti909 – 9091K → E in BAC98204. (PubMed:14621295)Curated
    Sequence conflicti963 – 9642QQ → PE in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti1084 – 10841S → Y in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti1084 – 10841S → Y in BAC98204. (PubMed:14621295)Curated
    Sequence conflicti1085 – 10851R → Q in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti1155 – 11551E → K in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti1182 – 11821Missing in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti1336 – 13394LWEA → SGKP in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti1487 – 14904DLQI → PAV in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti1653 – 16542EV → RI in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti1687 – 16871F → L in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti1694 – 16941I → V in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti1759 – 17591N → D in AAA83234. (PubMed:8834802)Curated
    Sequence conflicti1788 – 17881L → V in AAA83234. (PubMed:8834802)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1941 – 19499Missing in isoform 2. 1 PublicationVSP_010961

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U40342 mRNA. Translation: AAA83234.1. Frameshift.
    AK129394 mRNA. Translation: BAC98204.1. Different initiation.
    AC112146 Genomic DNA. No translation available.
    AC116574 Genomic DNA. No translation available.
    CCDSiCCDS36469.1. [Q61043-1]
    PIRiT30171.
    RefSeqiNP_001273008.1. NM_001286079.2. [Q61043-1]
    NP_001273009.1. NM_001286080.2. [Q61043-2]
    NP_032723.2. NM_008697.4. [Q61043-1]
    UniGeneiMm.244047.

    Genome annotation databases

    EnsembliENSMUST00000021468; ENSMUSP00000021468; ENSMUSG00000021068. [Q61043-1]
    ENSMUST00000095666; ENSMUSP00000093327; ENSMUSG00000021068. [Q61043-1]
    ENSMUST00000169074; ENSMUSP00000129648; ENSMUSG00000021068. [Q61043-1]
    GeneIDi18080.
    KEGGimmu:18080.
    UCSCiuc007ntg.1. mouse. [Q61043-1]
    uc007nth.1. mouse. [Q61043-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U40342 mRNA. Translation: AAA83234.1 . Frameshift.
    AK129394 mRNA. Translation: BAC98204.1 . Different initiation.
    AC112146 Genomic DNA. No translation available.
    AC116574 Genomic DNA. No translation available.
    CCDSi CCDS36469.1. [Q61043-1 ]
    PIRi T30171.
    RefSeqi NP_001273008.1. NM_001286079.2. [Q61043-1 ]
    NP_001273009.1. NM_001286080.2. [Q61043-2 ]
    NP_032723.2. NM_008697.4. [Q61043-1 ]
    UniGenei Mm.244047.

    3D structure databases

    ProteinModelPortali Q61043.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201772. 2 interactions.
    IntActi Q61043. 1 interaction.

    PTM databases

    PhosphoSitei Q61043.

    Proteomic databases

    PRIDEi Q61043.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021468 ; ENSMUSP00000021468 ; ENSMUSG00000021068 . [Q61043-1 ]
    ENSMUST00000095666 ; ENSMUSP00000093327 ; ENSMUSG00000021068 . [Q61043-1 ]
    ENSMUST00000169074 ; ENSMUSP00000129648 ; ENSMUSG00000021068 . [Q61043-1 ]
    GeneIDi 18080.
    KEGGi mmu:18080.
    UCSCi uc007ntg.1. mouse. [Q61043-1 ]
    uc007nth.1. mouse. [Q61043-2 ]

    Organism-specific databases

    CTDi 51199.
    MGIi MGI:105108. Nin.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG124644.
    GeneTreei ENSGT00660000095541.
    HOGENOMi HOG000293170.
    HOVERGENi HBG052623.
    KOi K16476.

    Miscellaneous databases

    ChiTaRSi NIN. mouse.
    NextBioi 293207.
    PROi Q61043.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61043.
    Bgeei Q61043.
    CleanExi MM_NIN.
    Genevestigatori Q61043.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    PROSITEi PS50222. EF_HAND_2. 5 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterisation of ninein, a new coiled-coil protein of the centrosome."
      Bouckson-Castaing V., Moudjou M., Ferguson D.J.P., Mucklow S., Belkaid Y., Milon G., Crocker P.R.
      J. Cell Sci. 109:179-190(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: C57BL/6.
      Tissue: Neonatal macrophage.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Embryonic tail.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "Microtubule minus-end anchorage at centrosomal and non-centrosomal sites: the role of ninein."
      Mogensen M.M., Malik A., Piel M., Bouckson-Castaing V., Bornens M.
      J. Cell Sci. 113:3013-3023(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiNIN_MOUSE
    AccessioniPrimary (citable) accession number: Q61043
    Secondary accession number(s): E9Q488, Q6ZPM7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 113 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3