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Protein

Ninein

Gene

Nin

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppressed release of microtubules (PubMed:10934040). Required for centriole organization and microtubule anchoring at the mother centriole (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi245 – 252GTPSequence analysis8
Nucleotide bindingi300 – 304GTPSequence analysis5
Nucleotide bindingi420 – 423GTPSequence analysis4

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • kinase binding Source: MGI

GO - Biological processi

  • centrosome localization Source: MGI
  • centrosome-templated microtubule nucleation Source: MGI
  • collateral sprouting Source: MGI
  • corpus callosum morphogenesis Source: MGI
  • corticospinal tract morphogenesis Source: MGI
  • microtubule anchoring at centrosome Source: UniProtKB
  • positive regulation of axonogenesis Source: MGI
  • positive regulation of microtubule polymerization Source: MGI
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ninein
Gene namesi
Name:Nin
Synonyms:Kiaa1565
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:105108. Nin.

Subcellular locationi

  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome 1 Publication
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity

  • Note: Component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization. Localizes to the subdistal appendage region of the centriole in a DCTN1-dependent manner.By similarity

GO - Cellular componenti

  • apical part of cell Source: MGI
  • axon Source: MGI
  • axonal growth cone Source: MGI
  • centriole Source: MGI
  • centrosome Source: HGNC
  • ciliary transition fiber Source: MGI
  • dendrite Source: MGI
  • microtubule Source: UniProtKB-KW
  • mitotic spindle pole Source: MGI
  • nucleolus Source: MGI
  • pericentriolar material Source: MGI
  • plasma membrane Source: MGI
  • spindle pole Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000968451 – 2035NineinAdd BLAST2035

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei152PhosphoserineBy similarity1
Modified residuei269PhosphoserineCombined sources1
Modified residuei1540PhosphoserineBy similarity1
Modified residuei1826PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/Aurora kinase B.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ61043.
PRIDEiQ61043.

PTM databases

iPTMnetiQ61043.
PhosphoSitePlusiQ61043.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in spleen, bone marrow and skin. Weakly expressed in liver and small intestine. Expressed in brain.1 Publication

Developmental stagei

Associated with the centrosome throughout the cell cycle. During mitosis, it is associated with the centrosome and the mitotic spindle. At anaphase, it is only localized to centrosomes.1 Publication

Gene expression databases

BgeeiENSMUSG00000021068.
CleanExiMM_NIN.
ExpressionAtlasiQ61043. baseline and differential.
GenevisibleiQ61043. MM.

Interactioni

Subunit structurei

Homooligomer. Interacts with GSK3B/GSK3-beta via its C-terminal domain. Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3B (By similarity). Interacts with AUNIP (via N-terminus) (By similarity). Identified in a complex with AUNIP and AURKA (By similarity).By similarity

GO - Molecular functioni

  • kinase binding Source: MGI

Protein-protein interaction databases

BioGridi201772. 2 interactors.
IntActiQ61043. 2 interactors.
STRINGi10090.ENSMUSP00000082422.

Structurei

3D structure databases

ProteinModelPortaliQ61043.
SMRiQ61043.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 43EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini42 – 77EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini182 – 217EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini219 – 252EF-hand 4PROSITE-ProRule annotationAdd BLAST34
Domaini317 – 352EF-hand 5PROSITE-ProRule annotationAdd BLAST36

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili357 – 1021Sequence analysisAdd BLAST665
Coiled coili1064 – 1094Sequence analysisAdd BLAST31
Coiled coili1178 – 1328Sequence analysisAdd BLAST151
Coiled coili1424 – 2013Sequence analysisAdd BLAST590

Domaini

The coiled coil region from Asn-1601 to Pro-1682 is necessary and sufficient for the targeting to centrosome.

Sequence similaritiesi

Contains 5 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IK59. Eukaryota.
ENOG410Z1RR. LUCA.
GeneTreeiENSGT00660000095541.
HOGENOMiHOG000293170.
HOVERGENiHBG052623.
InParanoidiQ61043.
KOiK16476.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR029664. NIN.
[Graphical view]
PANTHERiPTHR18905:SF11. PTHR18905:SF11. 4 hits.
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS50222. EF_HAND_2. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61043-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEVEEDQHE ARLKELFDSF DTLGTGSLGQ EELTDLCHVL CLEDVGPVLQ
60 70 80 90 100
QTLLQDNLLG RVHFDQFKEA LILILSRTLS SEEHFEESDC SPEAQPKYVR
110 120 130 140 150
GGKRYGRRSL PEFQESGEEI EEVTVLEPLE EEARSSPIPA GDCGEHWKTQ
160 170 180 190 200
RSEEYEAEGQ LRFWNPDDLN ASHGGSCPPP DWIEEKLQEV CEDLGITRDG
210 220 230 240 250
HLNRKKLVSI CEQYGLQNVD GAMLEEVFLS LDPDGTMSVE DFFYGLFKTG
260 270 280 290 300
KSLTPSASTP YRQLKRHLSM QSFDESGRRT ATSSAMTSTI GFRVFSCLDD
310 320 330 340 350
GMGQASVERI LDTWQEEGIE NSQEILKALD FSLDGNINLT ELTLALENEL
360 370 380 390 400
LVTKNGIHQA ALASFKAEIR HLLERVDQVV REKEKLRSDL DKAEKLKSLM
410 420 430 440 450
ASEVDDHHAA IERRNEYNLR KLDEEYKERI AALKNELRQE REQMLQQVGK
460 470 480 490 500
QRVELEQEIQ KAKTEENYIR DRLALSLKEN NRLETELLEN AEKLAEYESL
510 520 530 540 550
TQKLQRSLEN VLAEKFGDLD PSSAEFFLQE ERLAQMRNEY EQQCRLLQDQ
560 570 580 590 600
VDELQSELEE YQAQGRVLRL PLKNSLSEEL DGHSGGIEPD QGPGSEECNP
610 620 630 640 650
LNMSIEAELV IEQMKEQHHR DLCHLRLELE DKVRHYEKQL DDTRVASEQE
660 670 680 690 700
QAAMKQKYEQ GVHTLEKRVS ELRSEIADLE GQAAVLREAH HKASCRHEEE
710 720 730 740 750
KRQLQMAFDE EKAQLQEELR QEHERELQAR LQQAAESFRQ EREGLAQAAW
760 770 780 790 800
TEEKVRGLEQ SYQEQLLSLE EKHALEKEEL REELSEHHRR ELQEGREEME
810 820 830 840 850
TECNRRVSQI EAQCQADCEK VTEHCEQTLQ SLEVRHRQEL RDLLDQHLEE
860 870 880 890 900
RSQWEFEKDE LTQECTDAQE QLKEALQRER ATAAAMKQEQ EILERTYKDR
910 920 930 940 950
LNILSTERKQ LLQDLKDLQN ASESQHGLLS GQILELKRSQ ERELRDQGQA
960 970 980 990 1000
LCQTGVSEQL ASQQLERLRV EHEQERREMT GKLAALESAH RASLERADQE
1010 1020 1030 1040 1050
KAEMSTEICR LQNTVKDMQQ AASLLMLQGG CQATAGEEAE GDGAMSLLQQ
1060 1070 1080 1090 1100
GEQLLEENGD VLISLQRAHE HAVKENAKMA TEISRLQQRL KKLEPGSVIS
1110 1120 1130 1140 1150
SCLEEGTSEI SGSSREQVEP IMKQGPATKH FLSDLGDHEA RDLASTGTSS
1160 1170 1180 1190 1200
VQRQECKTEA SEASLDCFSE LENSEDTRTE SWDLKSQISQ LREQLTVLRA
1210 1220 1230 1240 1250
DCDRASERKQ DLLFDISVLK KKLKMLERLP EASSRYKVLY EDAARENSCL
1260 1270 1280 1290 1300
QEELRLVETR YEESLDSNKE LTAEVYRLQD EMKKMEEVME TFLSLEKSYD
1310 1320 1330 1340 1350
EVKVENEELR ALVLRLQGKM EKVLGRAALQ GDSYALWEAP SENLEVASDE
1360 1370 1380 1390 1400
KMLELRQTPK ECTPKVVSMH HIIEECTQET QCCEQGSTKL LARIKAHEIA
1410 1420 1430 1440 1450
WFHRAIKTHP EKPSAQNRVI PEGSAALLGL QDKHLQQEAT IAELELEKQK
1460 1470 1480 1490 1500
LQELTRNLRE RVTALVRQKD APSQGQKEEE LKAMMQDLQI TCGEMQRKVE
1510 1520 1530 1540 1550
LLRYESEKLQ EENSILRNEI TTLNEEDSIS NLKLEELNGS QEELWQKIET
1560 1570 1580 1590 1600
IEQEKASIQT MVEKLKKQVS DLKIKNQQLD SENIELSQKN SQNKEELKTL
1610 1620 1630 1640 1650
NQRLAEMLCQ REEPGACTSE KWEQENASLK EELDHYKVQT STLVSSLEAE
1660 1670 1680 1690 1700
LSEVKLQTHV MEQENLLLKD ELERLKQLHR CPDLSDFQQK MSSILSYNEK
1710 1720 1730 1740 1750
LLKEKEVLSE ELKSCADKLA ESSLLEHRIA TMKQEQTAWE EQSESLKSQL
1760 1770 1780 1790 1800
AVSQAKVQNL EDVLQNVNLQ MAEIESDLQV TRQEKEALKQ EVMSLHRQLQ
1810 1820 1830 1840 1850
NAIDKDWVSE TAPHLSGLRG QQRRLSWDKL DHLMNEEPQL LCQESKRLQT
1860 1870 1880 1890 1900
VVQNTQADLT HSREKVRQLE SNLLPTKHQK QLNQPCTVKS TEQEKLTLKR
1910 1920 1930 1940 1950
ECEQSQKEQS PTSRKVGQMG SLERGLETIH LENEGLKKKQ VRLDEKLMEM
1960 1970 1980 1990 2000
QPLRSTVTRS PSSHWDLQLL QQQACPMVPR EQFLQLQQQL LQAEKRSQHL
2010 2020 2030
QEELENRTSE TNTPQALLLE QRAVHADSCR RIGHL
Length:2,035
Mass (Da):234,723
Last modified:July 27, 2011 - v3
Checksum:iF92C1E77C1365D19
GO
Isoform 2 (identifier: Q61043-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1941-1949: Missing.

Note: No experimental confirmation available.
Show »
Length:2,026
Mass (Da):233,608
Checksum:iE7E37BD4108D2560
GO

Sequence cautioni

The sequence AAA83234 differs from that shown. Reason: Frameshift at position 2029.Curated
The sequence BAC98204 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145 – 146EH → DD in AAA83234 (PubMed:8834802).Curated2
Sequence conflicti341 – 342EL → DV in AAA83234 (PubMed:8834802).Curated2
Sequence conflicti383 – 394KEKLR…LDKAE → RRAYGRTWTSR in AAA83234 (PubMed:8834802).CuratedAdd BLAST12
Sequence conflicti506R → A in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti561Y → H in BAC98204 (PubMed:14621295).Curated1
Sequence conflicti737S → T in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti862T → A in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti909K → E in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti909K → E in BAC98204 (PubMed:14621295).Curated1
Sequence conflicti963 – 964QQ → PE in AAA83234 (PubMed:8834802).Curated2
Sequence conflicti1084S → Y in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti1084S → Y in BAC98204 (PubMed:14621295).Curated1
Sequence conflicti1085R → Q in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti1155E → K in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti1182Missing in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti1336 – 1339LWEA → SGKP in AAA83234 (PubMed:8834802).Curated4
Sequence conflicti1487 – 1490DLQI → PAV in AAA83234 (PubMed:8834802).Curated4
Sequence conflicti1653 – 1654EV → RI in AAA83234 (PubMed:8834802).Curated2
Sequence conflicti1687F → L in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti1694I → V in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti1759N → D in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti1788L → V in AAA83234 (PubMed:8834802).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0109611941 – 1949Missing in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40342 mRNA. Translation: AAA83234.1. Frameshift.
AK129394 mRNA. Translation: BAC98204.1. Different initiation.
AC112146 Genomic DNA. No translation available.
AC116574 Genomic DNA. No translation available.
CCDSiCCDS36469.1. [Q61043-1]
PIRiT30171.
RefSeqiNP_001273008.1. NM_001286079.2. [Q61043-1]
NP_001273009.1. NM_001286080.2. [Q61043-2]
NP_032723.2. NM_008697.4. [Q61043-1]
UniGeneiMm.244047.

Genome annotation databases

EnsembliENSMUST00000021468; ENSMUSP00000021468; ENSMUSG00000021068. [Q61043-1]
ENSMUST00000095666; ENSMUSP00000093327; ENSMUSG00000021068. [Q61043-1]
ENSMUST00000169074; ENSMUSP00000129648; ENSMUSG00000021068. [Q61043-1]
GeneIDi18080.
KEGGimmu:18080.
UCSCiuc007ntg.2. mouse. [Q61043-1]
uc007nth.2. mouse. [Q61043-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40342 mRNA. Translation: AAA83234.1. Frameshift.
AK129394 mRNA. Translation: BAC98204.1. Different initiation.
AC112146 Genomic DNA. No translation available.
AC116574 Genomic DNA. No translation available.
CCDSiCCDS36469.1. [Q61043-1]
PIRiT30171.
RefSeqiNP_001273008.1. NM_001286079.2. [Q61043-1]
NP_001273009.1. NM_001286080.2. [Q61043-2]
NP_032723.2. NM_008697.4. [Q61043-1]
UniGeneiMm.244047.

3D structure databases

ProteinModelPortaliQ61043.
SMRiQ61043.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201772. 2 interactors.
IntActiQ61043. 2 interactors.
STRINGi10090.ENSMUSP00000082422.

PTM databases

iPTMnetiQ61043.
PhosphoSitePlusiQ61043.

Proteomic databases

PaxDbiQ61043.
PRIDEiQ61043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021468; ENSMUSP00000021468; ENSMUSG00000021068. [Q61043-1]
ENSMUST00000095666; ENSMUSP00000093327; ENSMUSG00000021068. [Q61043-1]
ENSMUST00000169074; ENSMUSP00000129648; ENSMUSG00000021068. [Q61043-1]
GeneIDi18080.
KEGGimmu:18080.
UCSCiuc007ntg.2. mouse. [Q61043-1]
uc007nth.2. mouse. [Q61043-2]

Organism-specific databases

CTDi51199.
MGIiMGI:105108. Nin.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IK59. Eukaryota.
ENOG410Z1RR. LUCA.
GeneTreeiENSGT00660000095541.
HOGENOMiHOG000293170.
HOVERGENiHBG052623.
InParanoidiQ61043.
KOiK16476.

Miscellaneous databases

ChiTaRSiNin. mouse.
PROiQ61043.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021068.
CleanExiMM_NIN.
ExpressionAtlasiQ61043. baseline and differential.
GenevisibleiQ61043. MM.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR029664. NIN.
[Graphical view]
PANTHERiPTHR18905:SF11. PTHR18905:SF11. 4 hits.
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS50222. EF_HAND_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIN_MOUSE
AccessioniPrimary (citable) accession number: Q61043
Secondary accession number(s): E9Q488, Q6ZPM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.