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Protein

Ninein

Gene

Nin

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Centrosomal protein required for the positioning and anchorage of the microtubule minus-end in epithelial cells (PubMed:15784680, PubMed:10934040). May also act as a centrosome maturation factor (By similarity). May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome (PubMed:15784680). Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules (By similarity). Required for centriole organization and microtubule anchoring at the mother centriole (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi245 – 252GTPSequence analysis8
Nucleotide bindingi300 – 304GTPSequence analysis5
Nucleotide bindingi420 – 423GTPSequence analysis4

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • kinase binding Source: MGI

GO - Biological processi

  • centriole-centriole cohesion Source: Ensembl
  • centrosome localization Source: MGI
  • centrosome-templated microtubule nucleation Source: MGI
  • collateral sprouting Source: MGI
  • corpus callosum morphogenesis Source: MGI
  • corticospinal tract morphogenesis Source: MGI
  • microtubule anchoring at centrosome Source: Ensembl
  • positive regulation of axonogenesis Source: MGI
  • positive regulation of microtubule polymerization Source: MGI
  • protein localization Source: Ensembl

Keywordsi

LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ninein1 Publication
Gene namesi
Name:NinImported
Synonyms:Kiaa1565Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:105108 Nin

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000968451 – 2113NineinAdd BLAST2113

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei152PhosphoserineBy similarity1
Modified residuei269PhosphoserineCombined sources1
Modified residuei1540PhosphoserineBy similarity1
Modified residuei1826PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/Aurora kinase B.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ61043
PeptideAtlasiQ61043
PRIDEiQ61043

PTM databases

iPTMnetiQ61043
PhosphoSitePlusiQ61043

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in spleen, bone marrow and skin. Weakly expressed in liver and small intestine. Expressed in brain.1 Publication

Developmental stagei

Associated with the centrosome throughout the cell cycle (PubMed:8834802). During mitosis, it is associated with the centrosome and the mitotic spindle (PubMed:8834802). At anaphase, it is only localized to centrosomes (PubMed:8834802). Isoform 4 is highly expressed in postmitotic cortical neurons during neurogenesis (PubMed:27565344).2 Publications

Gene expression databases

BgeeiENSMUSG00000021068
CleanExiMM_NIN
ExpressionAtlasiQ61043 baseline and differential
GenevisibleiQ61043 MM

Interactioni

Subunit structurei

Homooligomer (By similarity). Interacts with GSK3B/GSK3-beta via its C-terminal domain (By similarity). Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3B (By similarity). Interacts with AUNIP (via N-terminus) (By similarity). Identified in a complex with AUNIP and AURKA (By similarity). Interacts with CCDC120 (By similarity). Interacts (via C-terminus) with CEP250 (PubMed:27565344). Interacts with CEP170 (PubMed:27565344). Interacts (via N-terminus) with the gamma-tubulin ring complex component TUBGCP3 (PubMed:15784680). Interacts with gamma-tubulin (PubMed:15784680). Isoform 4 does not interact with CEP170 or CEP250 (PubMed:27565344).By similarity2 Publications

GO - Molecular functioni

  • kinase binding Source: MGI

Protein-protein interaction databases

BioGridi201772, 3 interactors
IntActiQ61043, 2 interactors
MINTiQ61043
STRINGi10090.ENSMUSP00000082422

Structurei

3D structure databases

ProteinModelPortaliQ61043
SMRiQ61043
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 43EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini42 – 77EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini182 – 217EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini219 – 252EF-hand 4PROSITE-ProRule annotationAdd BLAST34
Domaini317 – 352EF-hand 5PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni798 – 1495Important for interaction with CEP1701 PublicationAdd BLAST698

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili358 – 570Sequence analysisAdd BLAST213
Coiled coili620 – 926Sequence analysisAdd BLAST307
Coiled coili958 – 1008Sequence analysisAdd BLAST51
Coiled coili1175 – 1323Sequence analysisAdd BLAST149
Coiled coili1425 – 1806Sequence analysisAdd BLAST382
Coiled coili1852 – 1910Sequence analysisAdd BLAST59
Coiled coili1971 – 2093Sequence analysisAdd BLAST123

Domaini

There is conflicting information regarding the regions required for centrosomal localization (PubMed:15784680). One study shows that the region 1591-1671 is necessary and sufficient for targeting to the centrosome (By similarity). Another study shows that a separate region within the coiled-coil domain, 1279-1565, is important for centrosomal localization (By similarity). However, a third study shows that the coiled-coil region (373-1874) is not sufficient for centrosomal localization and instead localizes to cytoplasmic speckles (PubMed:15784680). The observed differences might be due to oligomerization of the longer coiled-coil domain-containing sequence, which would mask the shorter centrosomal targeting sequences (PubMed:15784680).By similarity1 Publication
The N-terminal domain is important for targeting to the mother centriole, although it is not sufficient by itself for centrosomal localization.1 Publication

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IK59 Eukaryota
ENOG410Z1RR LUCA
GeneTreeiENSGT00660000095541
HOGENOMiHOG000293170
HOVERGENiHBG052623
InParanoidiQ61043
KOiK16476

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR002048 EF_hand_dom
IPR029664 NIN
PANTHERiPTHR18905:SF11 PTHR18905:SF11, 1 hit
SUPFAMiSSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS50222 EF_HAND_2, 5 hits

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61043-3) [UniParc]FASTAAdd to basket
Also known as: NPC-CT1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEVEEDQHE ARLKELFDSF DTLGTGSLGQ EELTDLCHVL CLEDVGPVLQ
60 70 80 90 100
QTLLQDNLLG RVHFDQFKEA LILILSRTLS SEEHFEESDC SPEAQPKYVR
110 120 130 140 150
GGKRYGRRSL PEFQESGEEI EEVTVLEPLE EEARSSPIPA GDCGEHWKTQ
160 170 180 190 200
RSEEYEAEGQ LRFWNPDDLN ASHGGSCPPP DWIEEKLQEV CEDLGITRDG
210 220 230 240 250
HLNRKKLVSI CEQYGLQNVD GAMLEEVFLS LDPDGTMSVE DFFYGLFKTG
260 270 280 290 300
KSLTPSASTP YRQLKRHLSM QSFDESGRRT ATSSAMTSTI GFRVFSCLDD
310 320 330 340 350
GMGQASVERI LDTWQEEGIE NSQEILKALD FSLDGNINLT ELTLALENEL
360 370 380 390 400
LVTKNGIHQA ALASFKAEIR HLLERVDQVV REKEKLRSDL DKAEKLKSLM
410 420 430 440 450
ASEVDDHHAA IERRNEYNLR KLDEEYKERI AALKNELRQE REQMLQQVGK
460 470 480 490 500
QRVELEQEIQ KAKTEENYIR DRLALSLKEN NRLETELLEN AEKLAEYESL
510 520 530 540 550
TQKLQRSLEN VLAEKFGDLD PSSAEFFLQE ERLAQMRNEY EQQCRLLQDQ
560 570 580 590 600
VDELQSELEE YQAQGRVLRL PLKNSLSEEL DGHSGGIEPD QGPGSEECNP
610 620 630 640 650
LNMSIEAELV IEQMKEQHHR DLCHLRLELE DKVRHYEKQL DDTRVASEQE
660 670 680 690 700
QAAMKQKYEQ GVHTLEKRVS ELRSEIADLE GQAAVLREAH HKASCRHEEE
710 720 730 740 750
KRQLQMAFDE EKAQLQEELR QEHERELQAR LQQAAESFRQ EREGLAQAAW
760 770 780 790 800
TEEKVRGLEQ SYQEQLLSLE EKHALEKEEL REELSEHHRR ELQEGREEME
810 820 830 840 850
TECNRRVSQI EAQCQADCEK VTEHCEQTLQ SLEVRHRQEL RDLLDQHLEE
860 870 880 890 900
RSQWEFEKDE LTQECTDAQE QLKEALQRER ATAAAMKQEQ EILERTYKDR
910 920 930 940 950
LNILSTERKQ LLQDLKDLQN ASESQHGLLS GQILELKRSQ ERELRDQGQA
960 970 980 990 1000
LCQTGVSEQL ASQQLERLRV EHEQERREMT GKLAALESAH RASLERADQE
1010 1020 1030 1040 1050
KAEMSTEICR LQNTVKDMQQ AASLLMLQGG CQATAGEEAE GDGAMSLLQQ
1060 1070 1080 1090 1100
GEQLLEENGD VLISLQRAHE HAVKENAKMA TEISRLQQRL KKLEPGSVIS
1110 1120 1130 1140 1150
SCLEEGTSEI SGSSREQVEP IMKQGPATKH FLSDLGDHEA RDLASTGTSS
1160 1170 1180 1190 1200
VQRQECKTEA SEASLDCFSE LENSEDTRTE SWDLKSQISQ LREQLTVLRA
1210 1220 1230 1240 1250
DCDRASERKQ DLLFDISVLK KKLKMLERLP EASSRYKVLY EDAARENSCL
1260 1270 1280 1290 1300
QEELRLVETR YEESLDSNKE LTAEVYRLQD EMKKMEEVME TFLSLEKSYD
1310 1320 1330 1340 1350
EVKVENEELR ALVLRLQGKM EKVLGRAALQ GDSYALWEAP SENLEVASDE
1360 1370 1380 1390 1400
KMLELRQTPK ECTPKVVSMH HIIEECTQET QCCEQGSTKL LARIKAHEIA
1410 1420 1430 1440 1450
WFHRAIKTHP EKPSAQNRVI PEGSAALLGL QDKHLQQEAT IAELELEKQK
1460 1470 1480 1490 1500
LQELTRNLRE RVTALVRQKD APSQGQKEEE LKAMMQDLQI TCGEMQRKVE
1510 1520 1530 1540 1550
LLRYESEKLQ EENSILRNEI TTLNEEDSIS NLKLEELNGS QEELWQKIET
1560 1570 1580 1590 1600
IEQEKASIQT MVEKLKKQVS DLKIKNQQLD SENIELSQKN SQNKEELKTL
1610 1620 1630 1640 1650
NQRLAEMLCQ REEPGACTSE KWEQENASLK EELDHYKVQT STLVSSLEAE
1660 1670 1680 1690 1700
LSEVKLQTHV MEQENLLLKD ELERLKQLHR CPDLSDFQQK MSSILSYNEK
1710 1720 1730 1740 1750
LLKEKEVLSE ELKSCADKLA ESSLLEHRIA TMKQEQTAWE EQSESLKSQL
1760 1770 1780 1790 1800
AVSQAKVQNL EDVLQNVNLQ MAEIESDLQV TRQEKEALKQ EVMSLHRQLQ
1810 1820 1830 1840 1850
NAIDKDWVSE TAPHLSGLRG QQRRLSWDKL DHLMNEEPQL LCQESKRLQT
1860 1870 1880 1890 1900
VVQNTQADLT HSREKVRQLE SNLLPTKHQK QLNQPCTVKS TEQEKLTLKR
1910 1920 1930 1940 1950
ECEQSQKEQS PTSRKVGQMG SLERGLETIH LENEGLKKKQ MQPLRSTVTR
1960 1970 1980 1990 2000
SPSSHWDLQL LQQQACPMVP REQFLQLQQQ LLQAEKRSQH LQEELENRTS
2010 2020 2030 2040 2050
ETNTPQGNQE HLVNLMEERM IEVEQKLKLV KRLLQEKVNQ LKEQLCKNTK
2060 2070 2080 2090 2100
TDAVVKDLYV ENAQLLKALE MTEQRQKTAE KRNFLLEEKI ASLSTIVRNL
2110
APAPLTSMPP LRS
Length:2,113
Mass (Da):243,718
Last modified:April 25, 2018 - v4
Checksum:iF3A2EEBD3E9FC826
GO
Isoform 2 (identifier: Q61043-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2007-2026: GNQEHLVNLMEERMIEVEQK → ALLLEQRAVHADSCRRIGHL
     2027-2113: Missing.

Note: PubMed:8834802 (AAA83234) sequence differs from that shown due a frameshift in position 2017.Curated
Show »
Length:2,026
Mass (Da):233,608
Checksum:iE7E37BD4108D2560
GO
Isoform 3 (identifier: Q61043-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1940-1940: Q → QVRLDEKLME
     2007-2026: GNQEHLVNLMEERMIEVEQK → ALLLEQRAVHADSCRRIGHL
     2027-2113: Missing.

Show »
Length:2,035
Mass (Da):234,723
Checksum:iF92C1E77C1365D19
GO
Isoform 4 (identifier: Q61043-4) [UniParc]FASTAAdd to basket
Also known as: Neuron1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     797-1503: Missing.
     1941-1979: MQPLRSTVTR...PREQFLQLQQ → VRLDEKLMES...DAAPEVHCDA
     1980-2113: Missing.

Show »
Length:1,272
Mass (Da):146,753
Checksum:i9807EC9ADFD01C46
GO

Sequence cautioni

The sequence AAA83234 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC98204 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145 – 146EH → DD in AAA83234 (PubMed:8834802).Curated2
Sequence conflicti341 – 342EL → DV in AAA83234 (PubMed:8834802).Curated2
Sequence conflicti383 – 394KEKLR…LDKAE → RRAYGRTWTSR in AAA83234 (PubMed:8834802).CuratedAdd BLAST12
Sequence conflicti506R → A in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti561Y → H in BAC98204 (PubMed:14621295).Curated1
Sequence conflicti675E → K in AAS87211 (PubMed:15784680).Curated1
Sequence conflicti737S → T in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti862T → A in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti909K → E in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti909K → E in BAC98204 (PubMed:14621295).Curated1
Sequence conflicti909K → E in AAI37790 (PubMed:15489334).Curated1
Sequence conflicti909K → E in AAI44914 (PubMed:15489334).Curated1
Sequence conflicti909K → E in AAS87211 (PubMed:15784680).Curated1
Sequence conflicti963 – 964QQ → PE in AAA83234 (PubMed:8834802).Curated2
Sequence conflicti1084 – 1085SR → YQ in AAA83234 (PubMed:8834802).Curated2
Sequence conflicti1084 – 1085SR → YQ in AAS87211 (PubMed:15784680).Curated2
Sequence conflicti1084S → Y in BAC98204 (PubMed:14621295).Curated1
Sequence conflicti1084S → Y in AAI37790 (PubMed:15489334).Curated1
Sequence conflicti1084S → Y in AAI44914 (PubMed:15489334).Curated1
Sequence conflicti1155E → K in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti1155E → K in AAS87211 (PubMed:15784680).Curated1
Sequence conflicti1182Missing in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti1327A → P in AAS87211 (PubMed:15784680).Curated1
Sequence conflicti1336 – 1339LWEA → SGKP in AAA83234 (PubMed:8834802).Curated4
Sequence conflicti1487 – 1490DLQI → PAV in AAA83234 (PubMed:8834802).Curated4
Sequence conflicti1653 – 1654EV → RI in AAA83234 (PubMed:8834802).Curated2
Sequence conflicti1687F → L in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti1694I → V in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti1759N → D in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti1788L → V in AAA83234 (PubMed:8834802).Curated1
Sequence conflicti1841L → F in AAI44914 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_059551797 – 1503Missing in isoform 4. Add BLAST707
Alternative sequenceiVSP_0595521940Q → QVRLDEKLME in isoform 3. 1
Alternative sequenceiVSP_0595531941 – 1979MQPLR…LQLQQ → VRLDEKLMESSVVGSSRAGR SSVPELACEDAAPEVHCDA in isoform 4. Add BLAST39
Alternative sequenceiVSP_0595541980 – 2113Missing in isoform 4. Add BLAST134
Alternative sequenceiVSP_0595552007 – 2026GNQEH…EVEQK → ALLLEQRAVHADSCRRIGHL in isoform 2 and isoform 3. Add BLAST20
Alternative sequenceiVSP_0595562027 – 2113Missing in isoform 2 and isoform 3. Add BLAST87

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40342 mRNA Translation: AAA83234.1 Sequence problems.
AY515727 mRNA Translation: AAS87211.1
AK129394 mRNA Translation: BAC98204.1 Different initiation.
AC112146 Genomic DNA No translation available.
AC116574 Genomic DNA No translation available.
BC137789 mRNA Translation: AAI37790.1
BC144913 mRNA Translation: AAI44914.1
CCDSiCCDS36469.1 [Q61043-3]
PIRiT30171
RefSeqiNP_001074922.1, NM_001081453.1
NP_001273008.1, NM_001286079.2 [Q61043-1]
NP_001273009.1, NM_001286080.2 [Q61043-2]
NP_032723.2, NM_008697.4 [Q61043-1]
XP_011242311.1, XM_011244009.1
UniGeneiMm.244047

Genome annotation databases

EnsembliENSMUST00000021468; ENSMUSP00000021468; ENSMUSG00000021068 [Q61043-1]
ENSMUST00000085314; ENSMUSP00000082422; ENSMUSG00000021068 [Q61043-3]
ENSMUST00000095666; ENSMUSP00000093327; ENSMUSG00000021068 [Q61043-1]
ENSMUST00000169074; ENSMUSP00000129648; ENSMUSG00000021068 [Q61043-1]
ENSMUST00000220689; ENSMUSP00000152530; ENSMUSG00000021068 [Q61043-4]
ENSMUST00000222237; ENSMUSP00000152240; ENSMUSG00000021068 [Q61043-3]
GeneIDi18080
KEGGimmu:18080
UCSCiuc007ntg.2 mouse [Q61043-3]
uc007nth.2 mouse [Q61043-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNIN_MOUSE
AccessioniPrimary (citable) accession number: Q61043
Secondary accession number(s): A0A1Y7VJL5
, B2RQ73, B7ZMZ9, E9Q488, E9Q4S3, Q674R4, Q6ZPM7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: April 25, 2018
Last modified: May 23, 2018
This is version 142 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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