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Q61043

- NIN_MOUSE

UniProt

Q61043 - NIN_MOUSE

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Protein

Ninein

Gene

Nin

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppressed release of microtubules.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi245 – 2528GTPSequence Analysis
Nucleotide bindingi300 – 3045GTPSequence Analysis
Nucleotide bindingi420 – 4234GTPSequence Analysis

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. GTP binding Source: UniProtKB-KW
  3. kinase binding Source: MGI

GO - Biological processi

  1. centrosome-templated microtubule nucleation Source: MGI
  2. microtubule anchoring at centrosome Source: MGI
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ninein
Gene namesi
Name:Nin
Synonyms:Kiaa1565
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:105108. Nin.

Subcellular locationi

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome 1 Publication
Note: Component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization.

GO - Cellular componenti

  1. centriole Source: MGI
  2. centrosome Source: HGNC
  3. microtubule Source: UniProtKB-KW
  4. spindle pole Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20352035NineinPRO_0000096845Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1540 – 15401PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/Aurora kinase B.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ61043.
PRIDEiQ61043.

PTM databases

PhosphoSiteiQ61043.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in spleen, bone marrow and skin. Weakly expressed in liver and small intestine. Expressed in brain.1 Publication

Developmental stagei

Associated with the centrosome throughout the cell cycle. During mitosis, it is associated with the centrosome and the mitotic spindle. At anaphase, it is only localized to centrosomes.1 Publication

Gene expression databases

BgeeiQ61043.
CleanExiMM_NIN.
ExpressionAtlasiQ61043. baseline and differential.
GenevestigatoriQ61043.

Interactioni

Subunit structurei

Homooligomer. Interacts with GSK3B/GSK3-beta via its C-terminal domain. Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3B (By similarity). Interacts with AUNIP (via N-terminus) (By similarity). Identified in a complex with AUNIP and AURKA (By similarity).By similarity

Protein-protein interaction databases

BioGridi201772. 2 interactions.
IntActiQ61043. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ61043.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 4336EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini42 – 7736EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini182 – 21736EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini219 – 25234EF-hand 4PROSITE-ProRule annotationAdd
BLAST
Domaini317 – 35236EF-hand 5PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili357 – 1021665Sequence AnalysisAdd
BLAST
Coiled coili1064 – 109431Sequence AnalysisAdd
BLAST
Coiled coili1178 – 1328151Sequence AnalysisAdd
BLAST
Coiled coili1424 – 2013590Sequence AnalysisAdd
BLAST

Domaini

The coiled coil region from Asn-1601 to Pro-1682 is necessary and sufficient for the targeting to centrosome.

Sequence similaritiesi

Contains 5 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG124644.
GeneTreeiENSGT00660000095541.
HOGENOMiHOG000293170.
HOVERGENiHBG052623.
InParanoidiQ61043.
KOiK16476.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR029664. NIN.
[Graphical view]
PANTHERiPTHR18905:SF11. PTHR18905:SF11. 1 hit.
PROSITEiPS50222. EF_HAND_2. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q61043-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEVEEDQHE ARLKELFDSF DTLGTGSLGQ EELTDLCHVL CLEDVGPVLQ
60 70 80 90 100
QTLLQDNLLG RVHFDQFKEA LILILSRTLS SEEHFEESDC SPEAQPKYVR
110 120 130 140 150
GGKRYGRRSL PEFQESGEEI EEVTVLEPLE EEARSSPIPA GDCGEHWKTQ
160 170 180 190 200
RSEEYEAEGQ LRFWNPDDLN ASHGGSCPPP DWIEEKLQEV CEDLGITRDG
210 220 230 240 250
HLNRKKLVSI CEQYGLQNVD GAMLEEVFLS LDPDGTMSVE DFFYGLFKTG
260 270 280 290 300
KSLTPSASTP YRQLKRHLSM QSFDESGRRT ATSSAMTSTI GFRVFSCLDD
310 320 330 340 350
GMGQASVERI LDTWQEEGIE NSQEILKALD FSLDGNINLT ELTLALENEL
360 370 380 390 400
LVTKNGIHQA ALASFKAEIR HLLERVDQVV REKEKLRSDL DKAEKLKSLM
410 420 430 440 450
ASEVDDHHAA IERRNEYNLR KLDEEYKERI AALKNELRQE REQMLQQVGK
460 470 480 490 500
QRVELEQEIQ KAKTEENYIR DRLALSLKEN NRLETELLEN AEKLAEYESL
510 520 530 540 550
TQKLQRSLEN VLAEKFGDLD PSSAEFFLQE ERLAQMRNEY EQQCRLLQDQ
560 570 580 590 600
VDELQSELEE YQAQGRVLRL PLKNSLSEEL DGHSGGIEPD QGPGSEECNP
610 620 630 640 650
LNMSIEAELV IEQMKEQHHR DLCHLRLELE DKVRHYEKQL DDTRVASEQE
660 670 680 690 700
QAAMKQKYEQ GVHTLEKRVS ELRSEIADLE GQAAVLREAH HKASCRHEEE
710 720 730 740 750
KRQLQMAFDE EKAQLQEELR QEHERELQAR LQQAAESFRQ EREGLAQAAW
760 770 780 790 800
TEEKVRGLEQ SYQEQLLSLE EKHALEKEEL REELSEHHRR ELQEGREEME
810 820 830 840 850
TECNRRVSQI EAQCQADCEK VTEHCEQTLQ SLEVRHRQEL RDLLDQHLEE
860 870 880 890 900
RSQWEFEKDE LTQECTDAQE QLKEALQRER ATAAAMKQEQ EILERTYKDR
910 920 930 940 950
LNILSTERKQ LLQDLKDLQN ASESQHGLLS GQILELKRSQ ERELRDQGQA
960 970 980 990 1000
LCQTGVSEQL ASQQLERLRV EHEQERREMT GKLAALESAH RASLERADQE
1010 1020 1030 1040 1050
KAEMSTEICR LQNTVKDMQQ AASLLMLQGG CQATAGEEAE GDGAMSLLQQ
1060 1070 1080 1090 1100
GEQLLEENGD VLISLQRAHE HAVKENAKMA TEISRLQQRL KKLEPGSVIS
1110 1120 1130 1140 1150
SCLEEGTSEI SGSSREQVEP IMKQGPATKH FLSDLGDHEA RDLASTGTSS
1160 1170 1180 1190 1200
VQRQECKTEA SEASLDCFSE LENSEDTRTE SWDLKSQISQ LREQLTVLRA
1210 1220 1230 1240 1250
DCDRASERKQ DLLFDISVLK KKLKMLERLP EASSRYKVLY EDAARENSCL
1260 1270 1280 1290 1300
QEELRLVETR YEESLDSNKE LTAEVYRLQD EMKKMEEVME TFLSLEKSYD
1310 1320 1330 1340 1350
EVKVENEELR ALVLRLQGKM EKVLGRAALQ GDSYALWEAP SENLEVASDE
1360 1370 1380 1390 1400
KMLELRQTPK ECTPKVVSMH HIIEECTQET QCCEQGSTKL LARIKAHEIA
1410 1420 1430 1440 1450
WFHRAIKTHP EKPSAQNRVI PEGSAALLGL QDKHLQQEAT IAELELEKQK
1460 1470 1480 1490 1500
LQELTRNLRE RVTALVRQKD APSQGQKEEE LKAMMQDLQI TCGEMQRKVE
1510 1520 1530 1540 1550
LLRYESEKLQ EENSILRNEI TTLNEEDSIS NLKLEELNGS QEELWQKIET
1560 1570 1580 1590 1600
IEQEKASIQT MVEKLKKQVS DLKIKNQQLD SENIELSQKN SQNKEELKTL
1610 1620 1630 1640 1650
NQRLAEMLCQ REEPGACTSE KWEQENASLK EELDHYKVQT STLVSSLEAE
1660 1670 1680 1690 1700
LSEVKLQTHV MEQENLLLKD ELERLKQLHR CPDLSDFQQK MSSILSYNEK
1710 1720 1730 1740 1750
LLKEKEVLSE ELKSCADKLA ESSLLEHRIA TMKQEQTAWE EQSESLKSQL
1760 1770 1780 1790 1800
AVSQAKVQNL EDVLQNVNLQ MAEIESDLQV TRQEKEALKQ EVMSLHRQLQ
1810 1820 1830 1840 1850
NAIDKDWVSE TAPHLSGLRG QQRRLSWDKL DHLMNEEPQL LCQESKRLQT
1860 1870 1880 1890 1900
VVQNTQADLT HSREKVRQLE SNLLPTKHQK QLNQPCTVKS TEQEKLTLKR
1910 1920 1930 1940 1950
ECEQSQKEQS PTSRKVGQMG SLERGLETIH LENEGLKKKQ VRLDEKLMEM
1960 1970 1980 1990 2000
QPLRSTVTRS PSSHWDLQLL QQQACPMVPR EQFLQLQQQL LQAEKRSQHL
2010 2020 2030
QEELENRTSE TNTPQALLLE QRAVHADSCR RIGHL
Length:2,035
Mass (Da):234,723
Last modified:July 27, 2011 - v3
Checksum:iF92C1E77C1365D19
GO
Isoform 2 (identifier: Q61043-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1941-1949: Missing.

Note: No experimental confirmation available.

Show »
Length:2,026
Mass (Da):233,608
Checksum:iE7E37BD4108D2560
GO

Sequence cautioni

The sequence AAA83234.1 differs from that shown. Reason: Frameshift at position 2029.
The sequence BAC98204.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti145 – 1462EH → DD in AAA83234. (PubMed:8834802)Curated
Sequence conflicti341 – 3422EL → DV in AAA83234. (PubMed:8834802)Curated
Sequence conflicti383 – 39412KEKLR…LDKAE → RRAYGRTWTSR in AAA83234. (PubMed:8834802)CuratedAdd
BLAST
Sequence conflicti506 – 5061R → A in AAA83234. (PubMed:8834802)Curated
Sequence conflicti561 – 5611Y → H in BAC98204. (PubMed:14621295)Curated
Sequence conflicti737 – 7371S → T in AAA83234. (PubMed:8834802)Curated
Sequence conflicti862 – 8621T → A in AAA83234. (PubMed:8834802)Curated
Sequence conflicti909 – 9091K → E in AAA83234. (PubMed:8834802)Curated
Sequence conflicti909 – 9091K → E in BAC98204. (PubMed:14621295)Curated
Sequence conflicti963 – 9642QQ → PE in AAA83234. (PubMed:8834802)Curated
Sequence conflicti1084 – 10841S → Y in AAA83234. (PubMed:8834802)Curated
Sequence conflicti1084 – 10841S → Y in BAC98204. (PubMed:14621295)Curated
Sequence conflicti1085 – 10851R → Q in AAA83234. (PubMed:8834802)Curated
Sequence conflicti1155 – 11551E → K in AAA83234. (PubMed:8834802)Curated
Sequence conflicti1182 – 11821Missing in AAA83234. (PubMed:8834802)Curated
Sequence conflicti1336 – 13394LWEA → SGKP in AAA83234. (PubMed:8834802)Curated
Sequence conflicti1487 – 14904DLQI → PAV in AAA83234. (PubMed:8834802)Curated
Sequence conflicti1653 – 16542EV → RI in AAA83234. (PubMed:8834802)Curated
Sequence conflicti1687 – 16871F → L in AAA83234. (PubMed:8834802)Curated
Sequence conflicti1694 – 16941I → V in AAA83234. (PubMed:8834802)Curated
Sequence conflicti1759 – 17591N → D in AAA83234. (PubMed:8834802)Curated
Sequence conflicti1788 – 17881L → V in AAA83234. (PubMed:8834802)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1941 – 19499Missing in isoform 2. 1 PublicationVSP_010961

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U40342 mRNA. Translation: AAA83234.1. Frameshift.
AK129394 mRNA. Translation: BAC98204.1. Different initiation.
AC112146 Genomic DNA. No translation available.
AC116574 Genomic DNA. No translation available.
CCDSiCCDS36469.1. [Q61043-1]
PIRiT30171.
RefSeqiNP_001273008.1. NM_001286079.2. [Q61043-1]
NP_001273009.1. NM_001286080.2. [Q61043-2]
NP_032723.2. NM_008697.4. [Q61043-1]
UniGeneiMm.244047.

Genome annotation databases

EnsembliENSMUST00000021468; ENSMUSP00000021468; ENSMUSG00000021068. [Q61043-1]
ENSMUST00000095666; ENSMUSP00000093327; ENSMUSG00000021068. [Q61043-1]
ENSMUST00000169074; ENSMUSP00000129648; ENSMUSG00000021068. [Q61043-1]
GeneIDi18080.
KEGGimmu:18080.
UCSCiuc007ntg.1. mouse. [Q61043-1]
uc007nth.1. mouse. [Q61043-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U40342 mRNA. Translation: AAA83234.1 . Frameshift.
AK129394 mRNA. Translation: BAC98204.1 . Different initiation.
AC112146 Genomic DNA. No translation available.
AC116574 Genomic DNA. No translation available.
CCDSi CCDS36469.1. [Q61043-1 ]
PIRi T30171.
RefSeqi NP_001273008.1. NM_001286079.2. [Q61043-1 ]
NP_001273009.1. NM_001286080.2. [Q61043-2 ]
NP_032723.2. NM_008697.4. [Q61043-1 ]
UniGenei Mm.244047.

3D structure databases

ProteinModelPortali Q61043.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201772. 2 interactions.
IntActi Q61043. 1 interaction.

PTM databases

PhosphoSitei Q61043.

Proteomic databases

MaxQBi Q61043.
PRIDEi Q61043.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021468 ; ENSMUSP00000021468 ; ENSMUSG00000021068 . [Q61043-1 ]
ENSMUST00000095666 ; ENSMUSP00000093327 ; ENSMUSG00000021068 . [Q61043-1 ]
ENSMUST00000169074 ; ENSMUSP00000129648 ; ENSMUSG00000021068 . [Q61043-1 ]
GeneIDi 18080.
KEGGi mmu:18080.
UCSCi uc007ntg.1. mouse. [Q61043-1 ]
uc007nth.1. mouse. [Q61043-2 ]

Organism-specific databases

CTDi 51199.
MGIi MGI:105108. Nin.
Rougei Search...

Phylogenomic databases

eggNOGi NOG124644.
GeneTreei ENSGT00660000095541.
HOGENOMi HOG000293170.
HOVERGENi HBG052623.
InParanoidi Q61043.
KOi K16476.

Miscellaneous databases

ChiTaRSi NIN. mouse.
NextBioi 293207.
PROi Q61043.
SOURCEi Search...

Gene expression databases

Bgeei Q61043.
CleanExi MM_NIN.
ExpressionAtlasi Q61043. baseline and differential.
Genevestigatori Q61043.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
InterProi IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR029664. NIN.
[Graphical view ]
PANTHERi PTHR18905:SF11. PTHR18905:SF11. 1 hit.
PROSITEi PS50222. EF_HAND_2. 5 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterisation of ninein, a new coiled-coil protein of the centrosome."
    Bouckson-Castaing V., Moudjou M., Ferguson D.J.P., Mucklow S., Belkaid Y., Milon G., Crocker P.R.
    J. Cell Sci. 109:179-190(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6.
    Tissue: Neonatal macrophage.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryonic tail.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "Microtubule minus-end anchorage at centrosomal and non-centrosomal sites: the role of ninein."
    Mogensen M.M., Malik A., Piel M., Bouckson-Castaing V., Bornens M.
    J. Cell Sci. 113:3013-3023(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNIN_MOUSE
AccessioniPrimary (citable) accession number: Q61043
Secondary accession number(s): E9Q488, Q6ZPM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3