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Q61043

- NIN_MOUSE

UniProt

Q61043 - NIN_MOUSE

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Protein

Ninein

Gene
Nin, Kiaa1565
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppressed release of microtubules.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi245 – 2528GTP Reviewed prediction
Nucleotide bindingi300 – 3045GTP Reviewed prediction
Nucleotide bindingi420 – 4234GTP Reviewed prediction

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. GTP binding Source: UniProtKB-KW
  3. kinase binding Source: MGI
  4. protein binding Source: MGI

GO - Biological processi

  1. centrosome-templated microtubule nucleation Source: MGI
  2. microtubule anchoring at centrosome Source: MGI
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ninein
Gene namesi
Name:Nin
Synonyms:Kiaa1565
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:105108. Nin.

Subcellular locationi

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome
Note: Component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization.1 Publication

GO - Cellular componenti

  1. centriole Source: MGI
  2. centrosome Source: HGNC
  3. microtubule Source: UniProtKB-KW
  4. spindle pole Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20352035NineinPRO_0000096845Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1540 – 15401Phosphoserine By similarity

Post-translational modificationi

Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/Aurora kinase B.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ61043.

PTM databases

PhosphoSiteiQ61043.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in spleen, bone marrow and skin. Weakly expressed in liver and small intestine. Expressed in brain.1 Publication

Developmental stagei

Associated with the centrosome throughout the cell cycle. During mitosis, it is associated with the centrosome and the mitotic spindle. At anaphase, it is only localized to centrosomes.1 Publication

Gene expression databases

ArrayExpressiQ61043.
BgeeiQ61043.
CleanExiMM_NIN.
GenevestigatoriQ61043.

Interactioni

Subunit structurei

Homooligomer. Interacts with GSK3B/GSK3-beta via its C-terminal domain. Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3B By similarity. Interacts with AUNIP (via N-terminus) By similarity. Identified in a complex with AUNIP and AURKA By similarity.

Protein-protein interaction databases

BioGridi201772. 2 interactions.
IntActiQ61043. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ61043.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 4336EF-hand 1Add
BLAST
Domaini42 – 7736EF-hand 2Add
BLAST
Domaini182 – 21736EF-hand 3Add
BLAST
Domaini219 – 25234EF-hand 4Add
BLAST
Domaini317 – 35236EF-hand 5Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili357 – 1021665 Reviewed predictionAdd
BLAST
Coiled coili1064 – 109431 Reviewed predictionAdd
BLAST
Coiled coili1178 – 1328151 Reviewed predictionAdd
BLAST
Coiled coili1424 – 2013590 Reviewed predictionAdd
BLAST

Domaini

The coiled coil region from Asn-1601 to Pro-1682 is necessary and sufficient for the targeting to centrosome.

Sequence similaritiesi

Contains 5 EF-hand domains.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG124644.
GeneTreeiENSGT00660000095541.
HOGENOMiHOG000293170.
HOVERGENiHBG052623.
KOiK16476.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PROSITEiPS50222. EF_HAND_2. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q61043-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDEVEEDQHE ARLKELFDSF DTLGTGSLGQ EELTDLCHVL CLEDVGPVLQ     50
QTLLQDNLLG RVHFDQFKEA LILILSRTLS SEEHFEESDC SPEAQPKYVR 100
GGKRYGRRSL PEFQESGEEI EEVTVLEPLE EEARSSPIPA GDCGEHWKTQ 150
RSEEYEAEGQ LRFWNPDDLN ASHGGSCPPP DWIEEKLQEV CEDLGITRDG 200
HLNRKKLVSI CEQYGLQNVD GAMLEEVFLS LDPDGTMSVE DFFYGLFKTG 250
KSLTPSASTP YRQLKRHLSM QSFDESGRRT ATSSAMTSTI GFRVFSCLDD 300
GMGQASVERI LDTWQEEGIE NSQEILKALD FSLDGNINLT ELTLALENEL 350
LVTKNGIHQA ALASFKAEIR HLLERVDQVV REKEKLRSDL DKAEKLKSLM 400
ASEVDDHHAA IERRNEYNLR KLDEEYKERI AALKNELRQE REQMLQQVGK 450
QRVELEQEIQ KAKTEENYIR DRLALSLKEN NRLETELLEN AEKLAEYESL 500
TQKLQRSLEN VLAEKFGDLD PSSAEFFLQE ERLAQMRNEY EQQCRLLQDQ 550
VDELQSELEE YQAQGRVLRL PLKNSLSEEL DGHSGGIEPD QGPGSEECNP 600
LNMSIEAELV IEQMKEQHHR DLCHLRLELE DKVRHYEKQL DDTRVASEQE 650
QAAMKQKYEQ GVHTLEKRVS ELRSEIADLE GQAAVLREAH HKASCRHEEE 700
KRQLQMAFDE EKAQLQEELR QEHERELQAR LQQAAESFRQ EREGLAQAAW 750
TEEKVRGLEQ SYQEQLLSLE EKHALEKEEL REELSEHHRR ELQEGREEME 800
TECNRRVSQI EAQCQADCEK VTEHCEQTLQ SLEVRHRQEL RDLLDQHLEE 850
RSQWEFEKDE LTQECTDAQE QLKEALQRER ATAAAMKQEQ EILERTYKDR 900
LNILSTERKQ LLQDLKDLQN ASESQHGLLS GQILELKRSQ ERELRDQGQA 950
LCQTGVSEQL ASQQLERLRV EHEQERREMT GKLAALESAH RASLERADQE 1000
KAEMSTEICR LQNTVKDMQQ AASLLMLQGG CQATAGEEAE GDGAMSLLQQ 1050
GEQLLEENGD VLISLQRAHE HAVKENAKMA TEISRLQQRL KKLEPGSVIS 1100
SCLEEGTSEI SGSSREQVEP IMKQGPATKH FLSDLGDHEA RDLASTGTSS 1150
VQRQECKTEA SEASLDCFSE LENSEDTRTE SWDLKSQISQ LREQLTVLRA 1200
DCDRASERKQ DLLFDISVLK KKLKMLERLP EASSRYKVLY EDAARENSCL 1250
QEELRLVETR YEESLDSNKE LTAEVYRLQD EMKKMEEVME TFLSLEKSYD 1300
EVKVENEELR ALVLRLQGKM EKVLGRAALQ GDSYALWEAP SENLEVASDE 1350
KMLELRQTPK ECTPKVVSMH HIIEECTQET QCCEQGSTKL LARIKAHEIA 1400
WFHRAIKTHP EKPSAQNRVI PEGSAALLGL QDKHLQQEAT IAELELEKQK 1450
LQELTRNLRE RVTALVRQKD APSQGQKEEE LKAMMQDLQI TCGEMQRKVE 1500
LLRYESEKLQ EENSILRNEI TTLNEEDSIS NLKLEELNGS QEELWQKIET 1550
IEQEKASIQT MVEKLKKQVS DLKIKNQQLD SENIELSQKN SQNKEELKTL 1600
NQRLAEMLCQ REEPGACTSE KWEQENASLK EELDHYKVQT STLVSSLEAE 1650
LSEVKLQTHV MEQENLLLKD ELERLKQLHR CPDLSDFQQK MSSILSYNEK 1700
LLKEKEVLSE ELKSCADKLA ESSLLEHRIA TMKQEQTAWE EQSESLKSQL 1750
AVSQAKVQNL EDVLQNVNLQ MAEIESDLQV TRQEKEALKQ EVMSLHRQLQ 1800
NAIDKDWVSE TAPHLSGLRG QQRRLSWDKL DHLMNEEPQL LCQESKRLQT 1850
VVQNTQADLT HSREKVRQLE SNLLPTKHQK QLNQPCTVKS TEQEKLTLKR 1900
ECEQSQKEQS PTSRKVGQMG SLERGLETIH LENEGLKKKQ VRLDEKLMEM 1950
QPLRSTVTRS PSSHWDLQLL QQQACPMVPR EQFLQLQQQL LQAEKRSQHL 2000
QEELENRTSE TNTPQALLLE QRAVHADSCR RIGHL 2035
Length:2,035
Mass (Da):234,723
Last modified:July 27, 2011 - v3
Checksum:iF92C1E77C1365D19
GO
Isoform 2 (identifier: Q61043-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1941-1949: Missing.

Note: No experimental confirmation available.

Show »
Length:2,026
Mass (Da):233,608
Checksum:iE7E37BD4108D2560
GO

Sequence cautioni

The sequence AAA83234.1 differs from that shown. Reason: Frameshift at position 2029.
The sequence BAC98204.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1941 – 19499Missing in isoform 2. VSP_010961

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti145 – 1462EH → DD in AAA83234. 1 Publication
Sequence conflicti341 – 3422EL → DV in AAA83234. 1 Publication
Sequence conflicti383 – 39412KEKLR…LDKAE → RRAYGRTWTSR in AAA83234. 1 PublicationAdd
BLAST
Sequence conflicti506 – 5061R → A in AAA83234. 1 Publication
Sequence conflicti561 – 5611Y → H in BAC98204. 1 Publication
Sequence conflicti737 – 7371S → T in AAA83234. 1 Publication
Sequence conflicti862 – 8621T → A in AAA83234. 1 Publication
Sequence conflicti909 – 9091K → E in AAA83234. 1 Publication
Sequence conflicti909 – 9091K → E in BAC98204. 1 Publication
Sequence conflicti963 – 9642QQ → PE in AAA83234. 1 Publication
Sequence conflicti1084 – 10841S → Y in AAA83234. 1 Publication
Sequence conflicti1084 – 10841S → Y in BAC98204. 1 Publication
Sequence conflicti1085 – 10851R → Q in AAA83234. 1 Publication
Sequence conflicti1155 – 11551E → K in AAA83234. 1 Publication
Sequence conflicti1182 – 11821Missing in AAA83234. 1 Publication
Sequence conflicti1336 – 13394LWEA → SGKP in AAA83234. 1 Publication
Sequence conflicti1487 – 14904DLQI → PAV in AAA83234. 1 Publication
Sequence conflicti1653 – 16542EV → RI in AAA83234. 1 Publication
Sequence conflicti1687 – 16871F → L in AAA83234. 1 Publication
Sequence conflicti1694 – 16941I → V in AAA83234. 1 Publication
Sequence conflicti1759 – 17591N → D in AAA83234. 1 Publication
Sequence conflicti1788 – 17881L → V in AAA83234. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U40342 mRNA. Translation: AAA83234.1. Frameshift.
AK129394 mRNA. Translation: BAC98204.1. Different initiation.
AC112146 Genomic DNA. No translation available.
AC116574 Genomic DNA. No translation available.
CCDSiCCDS36469.1. [Q61043-1]
PIRiT30171.
RefSeqiNP_001273008.1. NM_001286079.2. [Q61043-1]
NP_001273009.1. NM_001286080.2. [Q61043-2]
NP_032723.2. NM_008697.4. [Q61043-1]
UniGeneiMm.244047.

Genome annotation databases

EnsembliENSMUST00000021468; ENSMUSP00000021468; ENSMUSG00000021068. [Q61043-1]
ENSMUST00000095666; ENSMUSP00000093327; ENSMUSG00000021068. [Q61043-1]
ENSMUST00000169074; ENSMUSP00000129648; ENSMUSG00000021068. [Q61043-1]
GeneIDi18080.
KEGGimmu:18080.
UCSCiuc007ntg.1. mouse. [Q61043-1]
uc007nth.1. mouse. [Q61043-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U40342 mRNA. Translation: AAA83234.1 . Frameshift.
AK129394 mRNA. Translation: BAC98204.1 . Different initiation.
AC112146 Genomic DNA. No translation available.
AC116574 Genomic DNA. No translation available.
CCDSi CCDS36469.1. [Q61043-1 ]
PIRi T30171.
RefSeqi NP_001273008.1. NM_001286079.2. [Q61043-1 ]
NP_001273009.1. NM_001286080.2. [Q61043-2 ]
NP_032723.2. NM_008697.4. [Q61043-1 ]
UniGenei Mm.244047.

3D structure databases

ProteinModelPortali Q61043.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201772. 2 interactions.
IntActi Q61043. 1 interaction.

PTM databases

PhosphoSitei Q61043.

Proteomic databases

PRIDEi Q61043.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021468 ; ENSMUSP00000021468 ; ENSMUSG00000021068 . [Q61043-1 ]
ENSMUST00000095666 ; ENSMUSP00000093327 ; ENSMUSG00000021068 . [Q61043-1 ]
ENSMUST00000169074 ; ENSMUSP00000129648 ; ENSMUSG00000021068 . [Q61043-1 ]
GeneIDi 18080.
KEGGi mmu:18080.
UCSCi uc007ntg.1. mouse. [Q61043-1 ]
uc007nth.1. mouse. [Q61043-2 ]

Organism-specific databases

CTDi 51199.
MGIi MGI:105108. Nin.
Rougei Search...

Phylogenomic databases

eggNOGi NOG124644.
GeneTreei ENSGT00660000095541.
HOGENOMi HOG000293170.
HOVERGENi HBG052623.
KOi K16476.

Miscellaneous databases

ChiTaRSi NIN. mouse.
NextBioi 293207.
PROi Q61043.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q61043.
Bgeei Q61043.
CleanExi MM_NIN.
Genevestigatori Q61043.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
InterProi IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view ]
PROSITEi PS50222. EF_HAND_2. 5 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterisation of ninein, a new coiled-coil protein of the centrosome."
    Bouckson-Castaing V., Moudjou M., Ferguson D.J.P., Mucklow S., Belkaid Y., Milon G., Crocker P.R.
    J. Cell Sci. 109:179-190(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6.
    Tissue: Neonatal macrophage.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryonic tail.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "Microtubule minus-end anchorage at centrosomal and non-centrosomal sites: the role of ninein."
    Mogensen M.M., Malik A., Piel M., Bouckson-Castaing V., Bornens M.
    J. Cell Sci. 113:3013-3023(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNIN_MOUSE
AccessioniPrimary (citable) accession number: Q61043
Secondary accession number(s): E9Q488, Q6ZPM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi