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Protein

Histidine--tRNA ligase, cytoplasmic

Gene

Hars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytoplasmic histidine--tRNA ligase. Plays a role in axon guidance.By similarity

Catalytic activityi

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei157L-histidineBy similarity1
Binding sitei173L-histidineBy similarity1
Binding sitei177L-histidineBy similarity1
Binding sitei326L-histidineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.21 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine--tRNA ligase, cytoplasmic (EC:6.1.1.21)
Alternative name(s):
Histidyl-tRNA synthetase
Short name:
HisRS
Gene namesi
Name:Hars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:108087 Hars

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001363342 – 509Histidine--tRNA ligase, cytoplasmicAdd BLAST508

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei356PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ61035
MaxQBiQ61035
PaxDbiQ61035
PeptideAtlasiQ61035
PRIDEiQ61035

PTM databases

iPTMnetiQ61035
PhosphoSitePlusiQ61035
SwissPalmiQ61035

Expressioni

Gene expression databases

BgeeiENSMUSG00000001380
CleanExiMM_HARS
GenevisibleiQ61035 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001416

Structurei

3D structure databases

ProteinModelPortaliQ61035
SMRiQ61035
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 59WHEP-TRSPROSITE-ProRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni130 – 132L-histidine bindingBy similarity3
Regioni330 – 331L-histidine bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1936 Eukaryota
COG0124 LUCA
GeneTreeiENSGT00390000005922
HOGENOMiHOG000018075
HOVERGENiHBG002731
InParanoidiQ61035
KOiK01892
OMAiCDFDFIG
OrthoDBiEOG091G05P3
TreeFamiTF300652

Family and domain databases

CDDicd00859 HisRS_anticodon, 1 hit
Gene3Di3.40.50.8001 hit
InterProiView protein in InterPro
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR015807 His-tRNA-ligase
IPR004516 HisRS/HisZ
IPR033656 HisRS_anticodon
IPR009068 S15_NS1_RNA-bd
IPR000738 WHEP-TRS_dom
PANTHERiPTHR11476 PTHR11476, 1 hit
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF00458 WHEP-TRS, 1 hit
PIRSFiPIRSF001549 His-tRNA_synth, 1 hit
SMARTiView protein in SMART
SM00991 WHEP-TRS, 1 hit
SUPFAMiSSF47060 SSF47060, 1 hit
TIGRFAMsiTIGR00442 hisS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit
PS00762 WHEP_TRS_1, 1 hit
PS51185 WHEP_TRS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRAALEEL VRLQGAHVRG LKEQKASAEQ IEEEVTKLLK LKAQLGQDEG
60 70 80 90 100
KQKFVLKTPK GTRDYSPRQM AVREKVFDVI IRCFKRHGAE VIDTPVFELK
110 120 130 140 150
ETLTGKYGED SKLIYDLKDQ GGELLSLRYD LTVPFARYLA MNKLTNIKRY
160 170 180 190 200
HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG QFDPMIPDAE CLKIMCEILS
210 220 230 240 250
SLQIGNFLVK VNDRRILDGM FAVCGVPDSK FRTICSSVDK LDKVSWEEVK
260 270 280 290 300
NEMVGEKGLA PEVADRIGDY VQQHGGVSLV EQLLQDPKLS QNKQAVEGLG
310 320 330 340 350
DLKLLFEYLI LFGIDDKISF DLSLARGLDY YTGVIYEAVL LQMPTQAGEE
360 370 380 390 400
PLGVGSIAAG GRYDGLVGMF DPKGRKVPCV GLSIGVERIF SIVEQRLEAS
410 420 430 440 450
EEKVRTTETQ VLVASAQKKL LEERLKLVSE LWDAGIKAEL LYKKNPKLLN
460 470 480 490 500
QLQYCEEAGI PLVAIIGEQE LKDGVIKLRS VASREEVDVR REDLVEEIRR

RTNQPLSTC
Length:509
Mass (Da):57,432
Last modified:July 27, 2011 - v2
Checksum:i17AB4AA8FAE1C6F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti490R → Q in AAC52914 (PubMed:8921907).Curated1
Sequence conflicti508T → I in AAC52914 (PubMed:8921907).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39473 mRNA Translation: AAC52914.1
AK146922 mRNA Translation: BAE27533.1
AK166826 mRNA Translation: BAE39050.1
AK166892 mRNA Translation: BAE39097.1
CCDSiCCDS29164.1
PIRiJC5223
RefSeqiNP_032240.3, NM_008214.4
UniGeneiMm.10528

Genome annotation databases

EnsembliENSMUST00000001416; ENSMUSP00000001416; ENSMUSG00000001380
GeneIDi15115
KEGGimmu:15115
UCSCiuc008eop.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSYHC_MOUSE
AccessioniPrimary (citable) accession number: Q61035
Secondary accession number(s): Q3TKU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: February 28, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome