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Protein

Histidine--tRNA ligase, cytoplasmic

Gene

Hars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cytoplasmic histidine--tRNA ligase. Plays a role in axon guidance.By similarity

Catalytic activityi

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.21. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine--tRNA ligase, cytoplasmic (EC:6.1.1.21)
Alternative name(s):
Histidyl-tRNA synthetase
Short name:
HisRS
Gene namesi
Name:Hars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:108087. Hars.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 509508Histidine--tRNA ligase, cytoplasmicPRO_0000136334Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei356 – 3561PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ61035.
MaxQBiQ61035.
PaxDbiQ61035.
PeptideAtlasiQ61035.
PRIDEiQ61035.

PTM databases

iPTMnetiQ61035.
PhosphoSiteiQ61035.
SwissPalmiQ61035.

Expressioni

Gene expression databases

BgeeiENSMUSG00000001380.
CleanExiMM_HARS.
GenevisibleiQ61035. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001416.

Structurei

3D structure databases

ProteinModelPortaliQ61035.
SMRiQ61035. Positions 1-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 5957WHEP-TRSPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 WHEP-TRS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1936. Eukaryota.
COG0124. LUCA.
GeneTreeiENSGT00390000005922.
HOGENOMiHOG000018075.
HOVERGENiHBG002731.
InParanoidiQ61035.
KOiK01892.
OMAiTRDYGPQ.
OrthoDBiEOG091G05P3.
TreeFamiTF300652.

Family and domain databases

CDDicd00859. HisRS_anticodon. 1 hit.
Gene3Di1.10.287.10. 1 hit.
3.40.50.800. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
IPR033656. HisRS_anticodon.
IPR009068. S15_NS1_RNA-bd.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRAALEEL VRLQGAHVRG LKEQKASAEQ IEEEVTKLLK LKAQLGQDEG
60 70 80 90 100
KQKFVLKTPK GTRDYSPRQM AVREKVFDVI IRCFKRHGAE VIDTPVFELK
110 120 130 140 150
ETLTGKYGED SKLIYDLKDQ GGELLSLRYD LTVPFARYLA MNKLTNIKRY
160 170 180 190 200
HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG QFDPMIPDAE CLKIMCEILS
210 220 230 240 250
SLQIGNFLVK VNDRRILDGM FAVCGVPDSK FRTICSSVDK LDKVSWEEVK
260 270 280 290 300
NEMVGEKGLA PEVADRIGDY VQQHGGVSLV EQLLQDPKLS QNKQAVEGLG
310 320 330 340 350
DLKLLFEYLI LFGIDDKISF DLSLARGLDY YTGVIYEAVL LQMPTQAGEE
360 370 380 390 400
PLGVGSIAAG GRYDGLVGMF DPKGRKVPCV GLSIGVERIF SIVEQRLEAS
410 420 430 440 450
EEKVRTTETQ VLVASAQKKL LEERLKLVSE LWDAGIKAEL LYKKNPKLLN
460 470 480 490 500
QLQYCEEAGI PLVAIIGEQE LKDGVIKLRS VASREEVDVR REDLVEEIRR

RTNQPLSTC
Length:509
Mass (Da):57,432
Last modified:July 27, 2011 - v2
Checksum:i17AB4AA8FAE1C6F6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti490 – 4901R → Q in AAC52914 (PubMed:8921907).Curated
Sequence conflicti508 – 5081T → I in AAC52914 (PubMed:8921907).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39473 mRNA. Translation: AAC52914.1.
AK146922 mRNA. Translation: BAE27533.1.
AK166826 mRNA. Translation: BAE39050.1.
AK166892 mRNA. Translation: BAE39097.1.
CCDSiCCDS29164.1.
PIRiJC5223.
RefSeqiNP_032240.3. NM_008214.4.
UniGeneiMm.10528.

Genome annotation databases

EnsembliENSMUST00000001416; ENSMUSP00000001416; ENSMUSG00000001380.
GeneIDi15115.
KEGGimmu:15115.
UCSCiuc008eop.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39473 mRNA. Translation: AAC52914.1.
AK146922 mRNA. Translation: BAE27533.1.
AK166826 mRNA. Translation: BAE39050.1.
AK166892 mRNA. Translation: BAE39097.1.
CCDSiCCDS29164.1.
PIRiJC5223.
RefSeqiNP_032240.3. NM_008214.4.
UniGeneiMm.10528.

3D structure databases

ProteinModelPortaliQ61035.
SMRiQ61035. Positions 1-504.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001416.

PTM databases

iPTMnetiQ61035.
PhosphoSiteiQ61035.
SwissPalmiQ61035.

Proteomic databases

EPDiQ61035.
MaxQBiQ61035.
PaxDbiQ61035.
PeptideAtlasiQ61035.
PRIDEiQ61035.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001416; ENSMUSP00000001416; ENSMUSG00000001380.
GeneIDi15115.
KEGGimmu:15115.
UCSCiuc008eop.2. mouse.

Organism-specific databases

CTDi3035.
MGIiMGI:108087. Hars.

Phylogenomic databases

eggNOGiKOG1936. Eukaryota.
COG0124. LUCA.
GeneTreeiENSGT00390000005922.
HOGENOMiHOG000018075.
HOVERGENiHBG002731.
InParanoidiQ61035.
KOiK01892.
OMAiTRDYGPQ.
OrthoDBiEOG091G05P3.
TreeFamiTF300652.

Enzyme and pathway databases

BRENDAi6.1.1.21. 3474.

Miscellaneous databases

ChiTaRSiHars. mouse.
PROiQ61035.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001380.
CleanExiMM_HARS.
GenevisibleiQ61035. MM.

Family and domain databases

CDDicd00859. HisRS_anticodon. 1 hit.
Gene3Di1.10.287.10. 1 hit.
3.40.50.800. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
IPR033656. HisRS_anticodon.
IPR009068. S15_NS1_RNA-bd.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYHC_MOUSE
AccessioniPrimary (citable) accession number: Q61035
Secondary accession number(s): Q3TKU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.