Reviewed, UniProtKB/Swiss-Prot Q61033 (LAP2A_MOUSE)

Names and origin

Protein names

Recommended name:
    Lamina-associated polypeptide 2, isoforms alpha/zeta
Alternative name(s):
    Thymopoietin isoforms alpha/zeta
      Short name=TP alpha/zeta

Gene names

Name:	Tmpo
Synonyms:	Lap2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	693 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in the structural organization of the nucleus and in the post-mitotic nuclear assembly. Play an important role, together with LMNA, in the nuclear anchorage of RB1 By similarity.
Subunit structure	Interacts with LMNA, BANF1 and RB1 and with chromosomes. Associates directly or indirectly with lamins at specific cell-cycle stages By similarity.
Subcellular location	Nucleus By similarity. Note: Expressed diffusely throughout the nucleus By similarity.
Domain	Has two structurally independent, non-interacting domains: LEM-like (also called LAP2-N or LEM-D) and LEM (also called LAP2-C or LEM-B). LEM-like binds DNA while LEM interacts with BANF1 By similarity.
Post-translational modification	Phosphorylated in a mitose-specific manner By similarity.
Sequence similarities	Belongs to the LEM family. Contains 1 LEM domain. Contains 1 LEM-like domain.

Ontologies

Keywords
Cellular component	Chromosomal protein Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from direct assay. Source: MGI
Cellular component	chromosome Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from direct assay. Source: MGI
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform Alpha (identifier: Q61033-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Zeta (identifier: Q61033-2)

The sequence of this isoform differs from the canonical sequence as follows:
     188-225: GKKKEHKKVK...FFQGISFPEI → DSKIELKLEK...TEHNQVFVVL
     226-693: Missing.

Isoform Beta (identifier: Q61029-1)

The sequence of this isoform can be found in the external entry Q61029-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform Delta (identifier: Q61029-2)

The sequence of this isoform can be found in the external entry Q61029-2.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform Epsilon (identifier: Q61029-3)

The sequence of this isoform can be found in the external entry Q61029-3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform Gamma (identifier: Q61029-4)

The sequence of this isoform can be found in the external entry Q61029-4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 693

692

Lamina-associated polypeptide 2, isoforms alpha/zeta

PRO_0000206145

Regions

Domain

5 – 48

44

LEM-like

Domain

108 – 152

45

LEM

Region

49 – 107

59

Linker

Motif

190 – 196

7

Nuclear localization signal Potential

Amino acid modifications

Modified residue

66

1

Phosphoserine By similarity

Modified residue

67

1

Phosphoserine By similarity

Modified residue

74

1

Phosphothreonine By similarity

Modified residue

79

1

Phosphoserine By similarity

Modified residue

155

1

Phosphoserine By similarity

Modified residue

159

1

Phosphothreonine Ref.2

Modified residue

163

1

Phosphothreonine Ref.2

Modified residue

167

1

Phosphoserine By similarity

Modified residue

176

1

Phosphoserine By similarity

Modified residue

179

1

Phosphoserine By similarity

Modified residue

183

1

Phosphoserine By similarity

Modified residue

308

1

Phosphoserine By similarity

Modified residue

349

1

Phosphoserine By similarity

Modified residue

368

1

Phosphoserine By similarity

Modified residue

422

1

Phosphoserine Ref.2

Natural variations

Alternative sequence

188 – 225

38

GKKKE…SFPEI → DSKIELKLEKREPLKGRAKT PVTLKQRRTEHNQVFVVL in isoform Zeta.

VSP_010128

Alternative sequence

226 – 693

468

Missing in isoform Zeta.

VSP_010129

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

693

Helix Strand Turn

Details...

Helix

495 – 504

10

Helix

505 – 507

3

Beta strand

508 – 510

3

Turn

516 – 518

3

Helix

524 – 530

7

Helix

537 – 541

5

Helix

558 – 604

47

Helix

610 – 654

45

Beta strand

655 – 657

3

Helix

661 – 669

9

Beta strand

674 – 678

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E3668F15942429A4	FASTA	693	75,332
	10 20 30 40 50 60 MPEFLEDPSV LTKDKLKSEL VANNVTLPAG EQRKDVYVQL YLQHLTARNR PPLAAGANSK 70 80 90 100 110 120 GPPDFSSDEE RDATPVLGSG ASVGRGRGAV GRKATKKTDK PRLEDKDDLD VTELSNEELL 130 140 150 160 170 180 DQLVRYGVNP GPIVGTTRKL YEKKLLKLRE QGTESRSSTP LPTVSSSAEN TRQNGSNDSD 190 200 210 220 230 240 RYSDNDEGKK KEHKKVKSAR DCVPFSELAS TPSGAFFQGI SFPEISTRPP LGRTELQAAK 250 260 270 280 290 300 KVQTTKRDPP RETCTDTALP GRGRPHKLAP GRSLFIPSEL CYDRCVEKSS SPSSQREFAA 310 320 330 340 350 360 RLVSAAASPS LIRETTTTYS KEHSENICRG GKSRAQPLRA EEPGVSDQSV FSSEREVLQE 370 380 390 400 410 420 SERSQVISPP LAQAIRDYVN SLLVQGGVGS LPRSSDSVPT LDVENICKRL SQSSYQDSES 430 440 450 460 470 480 LSPPRKVPRL SEKPARGGDS GSCVAFQNTP GSEHRSSFAK SVVSHSLTTL GVEVSKPPPQ 490 500 510 520 530 540 HDKIEASEPS FPLHESILKV VEEEWQQIDR QLPSVACRYP VSSIEAARIL SVPKVDDEIL 550 560 570 580 590 600 GFISEATPRA ATQASSTESC DKHLDLALCR SYEAAASALQ IAAHTAFVAK SLQADISQAA 610 620 630 640 650 660 QIINSIPSDA QQALRILNRT YDAASYLCDA AFDEVRMSAC AMGSSTMGRR YLWLKDCKIS 670 680 690 PASKNKLTVA PFKGGTLFGG EVHKVIKKRG NKQ
	Isoform Zeta. Checksum: C821EDA73DF98888	FASTA	225	24,950
	10 20 30 40 50 60 MPEFLEDPSV LTKDKLKSEL VANNVTLPAG EQRKDVYVQL YLQHLTARNR PPLAAGANSK 70 80 90 100 110 120 GPPDFSSDEE RDATPVLGSG ASVGRGRGAV GRKATKKTDK PRLEDKDDLD VTELSNEELL 130 140 150 160 170 180 DQLVRYGVNP GPIVGTTRKL YEKKLLKLRE QGTESRSSTP LPTVSSSAEN TRQNGSNDSD 190 200 210 220 RYSDNDEDSK IELKLEKREP LKGRAKTPVT LKQRRTEHNQ VFVVL
	Isoform Beta. See Q61029.	FASTA	–	–
	Isoform Delta. See Q61029.	FASTA	–	–
	Isoform Epsilon. See Q61029.	FASTA	–	–
	Isoform Gamma. See Q61029.	FASTA	–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The characterization and localization of the mouse thymopoietin/lamina-associated polypeptide 2 gene and its alternatively spliced products." Berger R., Theodor L., Shoham J., Gokkel E., Brok-Simoni F., Avraham K.B., Copeland N.G., Jenkins N.A., Rechavi G., Simon A.J. Genome Res. 6:361-370(1996) [PubMed: 8743987] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA; BETA; GAMMA; DELTA; EPSILON AND ZETA). Strain: C57BL/6 X DBA/2. Tissue: Thymus.
[2]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-159; THR-163 AND SER-422, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U39073 mRNA. Translation: AAC52573.1.
U39078 mRNA. Translation: AAC52578.1.

IPI

IPI00126338.
IPI00798595.

UniGene

Mm.159684
Mm.439157

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2V0X	X-ray	2.20	A/B	459-692	[»]

SMR

Q61033. Positions 2-56, 102-158.

ModBase

Search...

PTM databases

PhosphoSite

Q61033.

Proteomic databases

PRIDE

Q61033.

Genome annotation databases

Ensembl

ENSMUSG00000019961. Mus musculus. [Contig view]

Organism-specific databases

MGI

MGI:106920. Tmpo.

Phylogenomic databases

HOGENOM

Q61033.

HOVERGEN

Q61033.

Gene expression databases

ArrayExpress

Q61033.

Bgee

Q61033.

CleanEx

MM_TMPO.

GermOnline

ENSMUSG00000019961. Mus musculus.

Family and domain databases

InterPro

IPR003887. LEM.
IPR013146. Thymopoietin_LEM.
[Graphical view]

Pfam

PF03020. LEM. 1 hit.
PF08198. Thymopoietin. 1 hit.
[Graphical view]

ProDom

PD004209. LEM. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00540. LEM. 1 hit.
[Graphical view]

PROSITE

PS50954. LEM. 1 hit.
PS50955. LEM_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

Entry information

Entry name

LAP2A_MOUSE

Accession

Primary (citable) accession number: Q61033
Secondary accession number(s): Q61028

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	January 23, 2007
Last modified:	May 26, 2009
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)