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Q61033

- LAP2A_MOUSE

UniProt

Q61033 - LAP2A_MOUSE

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Protein

Lamina-associated polypeptide 2, isoforms alpha/zeta

Gene

Tmpo

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in the structural organization of the nucleus and in the post-mitotic nuclear assembly. Plays an important role, together with LMNA, in the nuclear anchorage of RB1 By similarity.By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: MGI
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lamina-associated polypeptide 2, isoforms alpha/zeta
Alternative name(s):
Thymopoietin isoforms alpha/zeta
Short name:
TP alpha/zeta
Gene namesi
Name:Tmpo
Synonyms:Lap2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:106920. Tmpo.

Subcellular locationi

Nucleus By similarity. Chromosome By similarity
Note: Expressed diffusely throughout the nucleus.By similarity

GO - Cellular componenti

  1. chromatin Source: MGI
  2. nuclear envelope Source: MGI
  3. nuclear inner membrane Source: Ensembl
  4. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 693692Lamina-associated polypeptide 2, isoforms alpha/zetaPRO_0000206145Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei59 – 591PhosphoserineBy similarity
Modified residuei66 – 661Phosphoserine2 Publications
Modified residuei67 – 671Phosphoserine2 Publications
Modified residuei74 – 741Phosphothreonine2 Publications
Modified residuei79 – 791PhosphoserineBy similarity
Modified residuei153 – 1531PhosphothreonineBy similarity
Modified residuei155 – 1551PhosphoserineBy similarity
Modified residuei159 – 1591Phosphothreonine1 Publication
Modified residuei163 – 1631PhosphothreonineBy similarity
Modified residuei183 – 1831PhosphoserineBy similarity
Modified residuei349 – 3491PhosphoserineBy similarity
Modified residuei352 – 3521PhosphoserineBy similarity
Modified residuei368 – 3681PhosphoserineBy similarity
Modified residuei422 – 4221Phosphoserine1 Publication
Modified residuei655 – 6551N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated in a mitose-specific manner.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ61033.
PaxDbiQ61033.
PRIDEiQ61033.

Expressioni

Gene expression databases

BgeeiQ61033.
CleanExiMM_TMPO.
ExpressionAtlasiQ61033. baseline and differential.
GenevestigatoriQ61033.

Interactioni

Subunit structurei

Homooligomer. Interacts with LMNA, BANF1 and RB1 and with chromosomes. Associates directly or indirectly with lamins at specific cell-cycle stages By similarity.By similarity

Protein-protein interaction databases

BioGridi204234. 3 interactions.
DIPiDIP-29323N.
IntActiQ61033. 3 interactions.
MINTiMINT-4100192.

Structurei

Secondary structure

1
693
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi495 – 50410
Helixi505 – 5073
Beta strandi508 – 5103
Turni516 – 5183
Helixi524 – 5307
Helixi537 – 5415
Helixi558 – 60447
Helixi610 – 65445
Beta strandi655 – 6573
Helixi661 – 6699
Beta strandi674 – 6785

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V0XX-ray2.20A/B459-693[»]
ProteinModelPortaliQ61033.
SMRiQ61033. Positions 2-56, 102-158, 465-689.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ61033.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 4844LEM-likePROSITE-ProRule annotationAdd
BLAST
Domaini108 – 15245LEMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 10759LinkerAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili557 – 656100Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi190 – 1967Nuclear localization signalSequence Analysis

Domaini

The N-terminal part contains two structurally independent, non-interacting domains: LEM-like (also called LAP2-N or LEM-D) and LEM (also called LAP2-C or LEM-B). LEM-like binds DNA while LEM interacts with BANF1 By similarity.By similarity
The C-terminal domain forms a four-stranded coiled coil.1 Publication

Sequence similaritiesi

Belongs to the LEM family.Curated
Contains 1 LEM domain.PROSITE-ProRule annotation
Contains 1 LEM-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG87051.
GeneTreeiENSGT00510000048934.
HOGENOMiHOG000113280.
HOVERGENiHBG081890.
InParanoidiQ61033.
OMAiPRYRESK.
OrthoDBiEOG7KWSGZ.
TreeFamiTF328426.

Family and domain databases

Gene3Di1.10.720.40. 2 hits.
InterProiIPR021623. LAP2alpha.
IPR013146. LEM-like_dom.
IPR011015. LEM/LEM-like_dom.
IPR003887. LEM_dom.
[Graphical view]
PfamiPF11560. LAP2alpha. 1 hit.
PF03020. LEM. 1 hit.
PF08198. Thymopoietin. 1 hit.
[Graphical view]
SMARTiSM00540. LEM. 1 hit.
[Graphical view]
SUPFAMiSSF63451. SSF63451. 2 hits.
PROSITEiPS50954. LEM. 1 hit.
PS50955. LEM_LIKE. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform Alpha (identifier: Q61033-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPEFLEDPSV LTKDKLKSEL VANNVTLPAG EQRKDVYVQL YLQHLTARNR
60 70 80 90 100
PPLAAGANSK GPPDFSSDEE REPTPVLGSG ASVGRGRGAV GRKATKKTDK
110 120 130 140 150
PRLEDKDDLD VTELSNEELL DQLVRYGVNP GPIVGTTRKL YEKKLLKLRE
160 170 180 190 200
QGTESRSSTP LPTVSSSAEN TRQNGSNDSD RYSDNDEGKK KEHKKVKSAR
210 220 230 240 250
DCVPFSELAS TPSGAFFQGI SFPEISTRPP LGRTELQAAK KVQTTKRDPP
260 270 280 290 300
RETCTDTALP GKGQTHKLAP GRSLFIPSES SYDRCVEKSS SPSSQREFAA
310 320 330 340 350
RLVSAAASPS LIRETTTTYS KDIVENICRG GKSRAQPLRA EEPGVSDQSV
360 370 380 390 400
FSSEREVLQE SERSQVISPP LAQAIRDYVN SLLVQGGVGS LPGTSDSVPT
410 420 430 440 450
LDVENICKRL SQSSYQDSES LSPPRKVPRL SEKPARGGDS GSCVAFQNTP
460 470 480 490 500
GSEHRSSFAK SVVSHSLTTL GVEVSKPPPQ HDKIEASEPS FPLHESILKV
510 520 530 540 550
VEEEWQQIDR QLPSVACRYP VSSIEAARIL SVPKVDDEIL GFISEATPRA
560 570 580 590 600
ATQASSTESC DKHLDLALCR SYEAAASALQ IAAHTAFVAK SLQADISQAA
610 620 630 640 650
QIINSDPSDA QQALRILNRT YDAASYLCDA AFDEVRMSAC AMGSSTMGRR
660 670 680 690
YLWLKDCKIS PASKNKLTVA PFKGGTLFGG EVHKVIKKRG NKQ
Length:693
Mass (Da):75,168
Last modified:July 27, 2011 - v4
Checksum:i00604F3B66FF63F3
GO
Isoform Zeta (identifier: Q61033-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     188-225: GKKKEHKKVK...FFQGISFPEI → DSKIELKLEK...TEHNQVFVVL
     226-693: Missing.

Note: Contains a N6-acetyllysine at position 206.

Show »
Length:225
Mass (Da):24,990
Checksum:iD980460C9CE89898
GO
Isoform Beta (identifier: Q61029-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q61029.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:452
Mass (Da):50,373
GO
Isoform Delta (identifier: Q61029-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q61029.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:380
Mass (Da):42,650
GO
Isoform Epsilon (identifier: Q61029-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q61029.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:412
Mass (Da):46,050
GO
Isoform Gamma (identifier: Q61029-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q61029.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:343
Mass (Da):38,502
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 732EP → DA in AAC52573. (PubMed:8743987)Curated
Sequence conflicti72 – 732EP → DA in AAC52578. (PubMed:8743987)Curated
Sequence conflicti262 – 2654KGQT → RGRP in AAC52578. (PubMed:8743987)Curated
Sequence conflicti280 – 2812SS → LC in AAC52578. (PubMed:8743987)Curated
Sequence conflicti322 – 3243DIV → EHS in AAC52578. (PubMed:8743987)Curated
Sequence conflicti393 – 3942GT → RS in AAC52578. (PubMed:8743987)Curated
Sequence conflicti606 – 6061D → I in AAC52578. (PubMed:8743987)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei188 – 22538GKKKE…SFPEI → DSKIELKLEKREPLKGRAKT PVTLKQRRTEHNQVFVVL in isoform Zeta. 1 PublicationVSP_010128Add
BLAST
Alternative sequencei226 – 693468Missing in isoform Zeta. 1 PublicationVSP_010129Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U39073 mRNA. Translation: AAC52573.1.
U39078 mRNA. Translation: AAC52578.1.
CH466539 Genomic DNA. Translation: EDL21530.1.
BC141062 mRNA. Translation: AAI41063.1.
CCDSiCCDS24122.1. [Q61033-1]
RefSeqiNP_035735.2. NM_011605.3. [Q61033-1]
UniGeneiMm.159684.
Mm.439157.

Genome annotation databases

EnsembliENSMUST00000020123; ENSMUSP00000020123; ENSMUSG00000019961. [Q61033-1]
GeneIDi21917.
KEGGimmu:21917.
UCSCiuc007gtu.1. mouse. [Q61033-2]
uc007gtv.1. mouse. [Q61033-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U39073 mRNA. Translation: AAC52573.1 .
U39078 mRNA. Translation: AAC52578.1 .
CH466539 Genomic DNA. Translation: EDL21530.1 .
BC141062 mRNA. Translation: AAI41063.1 .
CCDSi CCDS24122.1. [Q61033-1 ]
RefSeqi NP_035735.2. NM_011605.3. [Q61033-1 ]
UniGenei Mm.159684.
Mm.439157.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2V0X X-ray 2.20 A/B 459-693 [» ]
ProteinModelPortali Q61033.
SMRi Q61033. Positions 2-56, 102-158, 465-689.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204234. 3 interactions.
DIPi DIP-29323N.
IntActi Q61033. 3 interactions.
MINTi MINT-4100192.

Proteomic databases

MaxQBi Q61033.
PaxDbi Q61033.
PRIDEi Q61033.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000020123 ; ENSMUSP00000020123 ; ENSMUSG00000019961 . [Q61033-1 ]
GeneIDi 21917.
KEGGi mmu:21917.
UCSCi uc007gtu.1. mouse. [Q61033-2 ]
uc007gtv.1. mouse. [Q61033-1 ]

Organism-specific databases

CTDi 7112.
MGIi MGI:106920. Tmpo.

Phylogenomic databases

eggNOGi NOG87051.
GeneTreei ENSGT00510000048934.
HOGENOMi HOG000113280.
HOVERGENi HBG081890.
InParanoidi Q61033.
OMAi PRYRESK.
OrthoDBi EOG7KWSGZ.
TreeFami TF328426.

Miscellaneous databases

ChiTaRSi TMPO. mouse.
EvolutionaryTracei Q61033.
NextBioi 301476.
PROi Q61033.
SOURCEi Search...

Gene expression databases

Bgeei Q61033.
CleanExi MM_TMPO.
ExpressionAtlasi Q61033. baseline and differential.
Genevestigatori Q61033.

Family and domain databases

Gene3Di 1.10.720.40. 2 hits.
InterProi IPR021623. LAP2alpha.
IPR013146. LEM-like_dom.
IPR011015. LEM/LEM-like_dom.
IPR003887. LEM_dom.
[Graphical view ]
Pfami PF11560. LAP2alpha. 1 hit.
PF03020. LEM. 1 hit.
PF08198. Thymopoietin. 1 hit.
[Graphical view ]
SMARTi SM00540. LEM. 1 hit.
[Graphical view ]
SUPFAMi SSF63451. SSF63451. 2 hits.
PROSITEi PS50954. LEM. 1 hit.
PS50955. LEM_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The characterization and localization of the mouse thymopoietin/lamina-associated polypeptide 2 gene and its alternatively spliced products."
    Berger R., Theodor L., Shoham J., Gokkel E., Brok-Simoni F., Avraham K.B., Copeland N.G., Jenkins N.A., Rechavi G., Simon A.J.
    Genome Res. 6:361-370(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA; BETA; GAMMA; DELTA; EPSILON AND ZETA).
    Strain: C57BL/6 X DBA/2.
    Tissue: Thymus.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66; SER-67; THR-74; THR-159 AND SER-422, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66; SER-67 AND THR-74, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-206 (ISOFORM ZETA), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  9. "Structural basis for dimerization of LAP2alpha, a component of the nuclear lamina."
    Bradley C.M., Jones S., Huang Y., Suzuki Y., Kvaratskhelia M., Hickman A.B., Craigie R., Dyda F.
    Structure 15:643-653(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 459-693, IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN, INTERACTION WITH LMNA, SUBUNIT.

Entry informationi

Entry nameiLAP2A_MOUSE
AccessioniPrimary (citable) accession number: Q61033
Secondary accession number(s): B2RUB9, Q61028
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 119 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3