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Protein

Lamina-associated polypeptide 2, isoforms alpha/zeta

Gene

Tmpo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in the structural organization of the nucleus and in the post-mitotic nuclear assembly. Plays an important role, together with LMNA, in the nuclear anchorage of RB1 (By similarity).By similarity

GO - Molecular functioni

  • cadherin binding Source: MGI
  • DNA binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct

GO - Biological processi

  • regulation of transcription, DNA-templated Source: MGI

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-2993913 Clearance of Nuclear Envelope Membranes from Chromatin
R-MMU-2995383 Initiation of Nuclear Envelope Reformation
R-MMU-4419969 Depolymerisation of the Nuclear Lamina

Names & Taxonomyi

Protein namesi
Recommended name:
Lamina-associated polypeptide 2, isoforms alpha/zeta
Alternative name(s):
Thymopoietin isoforms alpha/zeta
Short name:
TP alpha/zeta
Gene namesi
Name:Tmpo
Synonyms:Lap2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:106920 Tmpo

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002061451 – 693Lamina-associated polypeptide 2, isoforms alpha/zetaAdd BLAST693

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei59PhosphoserineBy similarity1
Modified residuei66PhosphoserineCombined sources1
Modified residuei67PhosphoserineCombined sources1
Modified residuei74PhosphothreonineCombined sources1
Modified residuei79PhosphoserineBy similarity1
Modified residuei82PhosphoserineCombined sources1
Modified residuei85Omega-N-methylarginineCombined sources1
Modified residuei87Omega-N-methylarginineCombined sources1
Modified residuei153PhosphothreonineCombined sources1
Modified residuei155PhosphoserineCombined sources1
Modified residuei158PhosphoserineCombined sources1
Modified residuei159PhosphothreonineCombined sources1
Modified residuei163PhosphothreonineBy similarity1
Modified residuei165PhosphoserineCombined sources1
Modified residuei167PhosphoserineCombined sources1
Modified residuei310PhosphoserineBy similarity1
Modified residuei329Omega-N-methylarginineCombined sources1
Modified residuei349PhosphoserineBy similarity1
Modified residuei352PhosphoserineBy similarity1
Modified residuei368PhosphoserineBy similarity1
Modified residuei420PhosphoserineCombined sources1
Modified residuei422PhosphoserineCombined sources1
Modified residuei655N6-acetyllysineBy similarity1
Isoform Zeta (identifier: Q61033-2)
Modified residuei206N6-acetyllysineCombined sources1

Post-translational modificationi

Phosphorylated in a mitose-specific manner.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ61033
MaxQBiQ61033
PaxDbiQ61033
PeptideAtlasiQ61033
PRIDEiQ61033

PTM databases

iPTMnetiQ61033

Expressioni

Gene expression databases

BgeeiENSMUSG00000019961
CleanExiMM_TMPO
ExpressionAtlasiQ61033 baseline and differential
GenevisibleiQ61033 MM

Interactioni

Subunit structurei

Homooligomer. Interacts with LMNA, BANF1 and RB1 and with chromosomes. Associates directly or indirectly with lamins at specific cell-cycle stages (By similarity).By similarity

GO - Molecular functioni

  • cadherin binding Source: MGI
  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi204234, 6 interactors
DIPiDIP-29323N
IntActiQ61033, 4 interactors
MINTiQ61033
STRINGi10090.ENSMUSP00000020123

Structurei

Secondary structure

1693
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi495 – 504Combined sources10
Helixi505 – 507Combined sources3
Beta strandi508 – 510Combined sources3
Turni516 – 518Combined sources3
Helixi524 – 530Combined sources7
Helixi537 – 541Combined sources5
Helixi558 – 604Combined sources47
Helixi610 – 654Combined sources45
Beta strandi655 – 657Combined sources3
Helixi661 – 669Combined sources9
Beta strandi674 – 678Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V0XX-ray2.20A/B459-693[»]
ProteinModelPortaliQ61033
SMRiQ61033
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ61033

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 48LEM-likePROSITE-ProRule annotationAdd BLAST44
Domaini108 – 152LEMPROSITE-ProRule annotationAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 107LinkerAdd BLAST59

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili557 – 656Add BLAST100

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi190 – 196Nuclear localization signalSequence analysis7

Domaini

The N-terminal part contains two structurally independent, non-interacting domains: LEM-like (also called LAP2-N or LEM-D) and LEM (also called LAP2-C or LEM-B). LEM-like binds DNA while LEM interacts with BANF1 (By similarity).By similarity
The C-terminal domain forms a four-stranded coiled coil.1 Publication

Sequence similaritiesi

Belongs to the LEM family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IT7C Eukaryota
ENOG4111J0N LUCA
GeneTreeiENSGT00510000048934
HOGENOMiHOG000113280
HOVERGENiHBG081890
InParanoidiQ61033
OrthoDBiEOG091G03A8
TreeFamiTF328426

Family and domain databases

Gene3Di1.10.720.40, 2 hits
InterProiView protein in InterPro
IPR021623 LAP2alpha_C
IPR013146 LEM-like_dom
IPR011015 LEM/LEM-like_dom_sf
IPR003887 LEM_dom
PfamiView protein in Pfam
PF11560 LAP2alpha, 1 hit
PF03020 LEM, 1 hit
PF08198 Thymopoietin, 1 hit
SMARTiView protein in SMART
SM00540 LEM, 1 hit
SM01261 Thymopoietin, 1 hit
SUPFAMiSSF63451 SSF63451, 2 hits
PROSITEiView protein in PROSITE
PS50954 LEM, 1 hit
PS50955 LEM_LIKE, 1 hit

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q61033-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPEFLEDPSV LTKDKLKSEL VANNVTLPAG EQRKDVYVQL YLQHLTARNR
60 70 80 90 100
PPLAAGANSK GPPDFSSDEE REPTPVLGSG ASVGRGRGAV GRKATKKTDK
110 120 130 140 150
PRLEDKDDLD VTELSNEELL DQLVRYGVNP GPIVGTTRKL YEKKLLKLRE
160 170 180 190 200
QGTESRSSTP LPTVSSSAEN TRQNGSNDSD RYSDNDEGKK KEHKKVKSAR
210 220 230 240 250
DCVPFSELAS TPSGAFFQGI SFPEISTRPP LGRTELQAAK KVQTTKRDPP
260 270 280 290 300
RETCTDTALP GKGQTHKLAP GRSLFIPSES SYDRCVEKSS SPSSQREFAA
310 320 330 340 350
RLVSAAASPS LIRETTTTYS KDIVENICRG GKSRAQPLRA EEPGVSDQSV
360 370 380 390 400
FSSEREVLQE SERSQVISPP LAQAIRDYVN SLLVQGGVGS LPGTSDSVPT
410 420 430 440 450
LDVENICKRL SQSSYQDSES LSPPRKVPRL SEKPARGGDS GSCVAFQNTP
460 470 480 490 500
GSEHRSSFAK SVVSHSLTTL GVEVSKPPPQ HDKIEASEPS FPLHESILKV
510 520 530 540 550
VEEEWQQIDR QLPSVACRYP VSSIEAARIL SVPKVDDEIL GFISEATPRA
560 570 580 590 600
ATQASSTESC DKHLDLALCR SYEAAASALQ IAAHTAFVAK SLQADISQAA
610 620 630 640 650
QIINSDPSDA QQALRILNRT YDAASYLCDA AFDEVRMSAC AMGSSTMGRR
660 670 680 690
YLWLKDCKIS PASKNKLTVA PFKGGTLFGG EVHKVIKKRG NKQ
Length:693
Mass (Da):75,168
Last modified:July 27, 2011 - v4
Checksum:i00604F3B66FF63F3
GO
Isoform Zeta (identifier: Q61033-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     188-225: GKKKEHKKVK...FFQGISFPEI → DSKIELKLEK...TEHNQVFVVL
     226-693: Missing.

Show »
Length:225
Mass (Da):24,990
Checksum:iD980460C9CE89898
GO
Isoform Beta (identifier: Q61029-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q61029.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:452
Mass (Da):50,373
GO
Isoform Delta (identifier: Q61029-2) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q61029.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:380
Mass (Da):42,650
GO
Isoform Epsilon (identifier: Q61029-3) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q61029.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:412
Mass (Da):46,050
GO
Isoform Gamma (identifier: Q61029-4) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q61029.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:343
Mass (Da):38,502
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72 – 73EP → DA in AAC52573 (PubMed:8743987).Curated2
Sequence conflicti72 – 73EP → DA in AAC52578 (PubMed:8743987).Curated2
Sequence conflicti262 – 265KGQT → RGRP in AAC52578 (PubMed:8743987).Curated4
Sequence conflicti280 – 281SS → LC in AAC52578 (PubMed:8743987).Curated2
Sequence conflicti322 – 324DIV → EHS in AAC52578 (PubMed:8743987).Curated3
Sequence conflicti393 – 394GT → RS in AAC52578 (PubMed:8743987).Curated2
Sequence conflicti606D → I in AAC52578 (PubMed:8743987).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010128188 – 225GKKKE…SFPEI → DSKIELKLEKREPLKGRAKT PVTLKQRRTEHNQVFVVL in isoform Zeta. 1 PublicationAdd BLAST38
Alternative sequenceiVSP_010129226 – 693Missing in isoform Zeta. 1 PublicationAdd BLAST468

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39073 mRNA Translation: AAC52573.1
U39078 mRNA Translation: AAC52578.1
CH466539 Genomic DNA Translation: EDL21530.1
BC141062 mRNA Translation: AAI41063.1
CCDSiCCDS24122.1 [Q61033-1]
RefSeqiNP_035735.2, NM_011605.3 [Q61033-1]
UniGeneiMm.159684
Mm.439157

Genome annotation databases

EnsembliENSMUST00000020123; ENSMUSP00000020123; ENSMUSG00000019961 [Q61033-1]
GeneIDi21917
UCSCiuc007gtu.2 mouse [Q61033-2]
uc007gtv.2 mouse [Q61033-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiLAP2A_MOUSE
AccessioniPrimary (citable) accession number: Q61033
Secondary accession number(s): B2RUB9, Q61028
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 150 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health