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Protein

Lamina-associated polypeptide 2, isoforms beta/delta/epsilon/gamma

Gene

Tmpo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May help direct the assembly of the nuclear lamina and thereby help maintain the structural organization of the nuclear envelope. Possible receptor for attachment of lamin filaments to the inner nuclear membrane. May be involved in the control of initiation of DNA replication through its interaction with NAKAP95 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: MGI

Keywordsi

Molecular functionDNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lamina-associated polypeptide 2, isoforms beta/delta/epsilon/gamma
Alternative name(s):
Thymopoietin isoforms beta/delta/epsilon/gamma
Short name:
TP beta/delta/epsilon/gamma
Gene namesi
Name:Tmpo
Synonyms:Lap2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:106920. Tmpo.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei410 – 430Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini431 – 452LumenalSequence analysisAdd BLAST22

Keywords - Cellular componenti

Chromosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002061461 – 452Lamina-associated polypeptide 2, isoforms beta/delta/epsilon/gammaAdd BLAST452

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei59PhosphoserineBy similarity1
Modified residuei66PhosphoserineCombined sources1
Modified residuei67PhosphoserineCombined sources1
Modified residuei74PhosphothreonineCombined sources1 Publication1
Modified residuei79PhosphoserineBy similarity1
Modified residuei82PhosphoserineCombined sources1
Modified residuei85Omega-N-methylarginineCombined sources1
Modified residuei87Omega-N-methylarginineCombined sources1
Modified residuei153PhosphothreonineCombined sources1
Modified residuei155PhosphoserineCombined sources1
Modified residuei158PhosphoserineCombined sources1
Modified residuei159PhosphothreonineCombined sources1 Publication1
Modified residuei163PhosphothreonineBy similarity1
Modified residuei165PhosphoserineCombined sources1
Modified residuei167PhosphoserineCombined sources1
Modified residuei176Phosphoserine1 Publication1
Modified residuei179Phosphoserine; by PKC1 Publication1
Modified residuei183PhosphoserineBy similarity1
Modified residuei189PhosphoserineBy similarity1
Modified residuei206N6-acetyllysineCombined sources1
Modified residuei210PhosphothreonineBy similarity1
Modified residuei221PhosphoserineBy similarity1
Modified residuei223PhosphoserineBy similarity1
Modified residuei249PhosphoserineBy similarity1
Modified residuei253PhosphoserineBy similarity1
Modified residuei264PhosphoserineBy similarity1
Modified residuei291PhosphoserineBy similarity1
Modified residuei305PhosphoserineCombined sources1
Modified residuei306PhosphoserineCombined sources1
Modified residuei311PhosphothreonineBy similarity1
Modified residuei314PhosphoserineBy similarity1
Modified residuei319Citrulline1 Publication1
Modified residuei361PhosphoserineCombined sources1
Modified residuei377PhosphoserineBy similarity1
Modified residuei384PhosphoserineBy similarity1
Modified residuei388N6-acetyllysineCombined sources1
Cross-linki400Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei401PhosphoserineBy similarity1
Isoform Zeta (identifier: Q61033-2)
Modified residuei206N6-acetyllysineCombined sources1

Post-translational modificationi

Mitosis-specific phosphorylation specifically abolishes its binding to lamin B and chromosomes.By similarity
Citrullinated by PADI4.1 Publication

Keywords - PTMi

Acetylation, Citrullination, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61029.
PaxDbiQ61029.
PeptideAtlasiQ61029.
PRIDEiQ61029.
TopDownProteomicsiQ61029-1. [Q61029-1]

PTM databases

iPTMnetiQ61029.
PhosphoSitePlusiQ61029.

Expressioni

Gene expression databases

BgeeiENSMUSG00000019961.
CleanExiMM_TMPO.
ExpressionAtlasiQ61029. baseline and differential.
GenevisibleiQ61029. MM.

Interactioni

Subunit structurei

Interacts with LMNB1, LMNB2, BANF1, AKAP8L, GMCL and chromosomes.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Zbtb7bQ643212EBI-6172136,EBI-642868

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204234. 5 interactors.
IntActiQ61029. 4 interactors.
MINTiMINT-4109431.

Structurei

3D structure databases

ProteinModelPortaliQ61029.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 48LEM-likePROSITE-ProRule annotationAdd BLAST44
Domaini109 – 153LEMPROSITE-ProRule annotationAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 409NucleoplasmicSequence analysisAdd BLAST409
Regioni49 – 108LinkerAdd BLAST60
Regioni137 – 242NAKAP95-binding NAdd BLAST106
Regioni298 – 370Binds lamins BAdd BLAST73
Regioni299 – 373NAKAP95-binding CAdd BLAST75

Domaini

Has two structurally independent, non-interacting domains: LEM-like (also called LAP2-N or LEM-D) and LEM (also called LAP2-C or LEM-B). LEM-like binds DNA while LEM interacts with BANF1 (By similarity).By similarity

Sequence similaritiesi

Belongs to the LEM family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IT7C. Eukaryota.
ENOG4111J0N. LUCA.
GeneTreeiENSGT00510000048934.
HOGENOMiHOG000113280.
HOVERGENiHBG000166.
OMAiTNQGNPF.

Family and domain databases

Gene3Di1.10.720.40. 1 hit.
InterProiView protein in InterPro
IPR013146. LEM-like_dom.
IPR011015. LEM/LEM-like_dom_sf.
IPR003887. LEM_dom.
PfamiView protein in Pfam
PF03020. LEM. 1 hit.
PF08198. Thymopoietin. 1 hit.
SMARTiView protein in SMART
SM00540. LEM. 1 hit.
SM01261. Thymopoietin. 1 hit.
SUPFAMiSSF63451. SSF63451. 2 hits.
PROSITEiView protein in PROSITE
PS50954. LEM. 1 hit.
PS50955. LEM_LIKE. 1 hit.

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Beta (identifier: Q61029-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPEFLEDPSV LTKDKLKSEL VANNVTLPAG EQRKDVYVQL YLQHLTARNR
60 70 80 90 100
PPLAAGANSK GPPDFSSDEE REPTPVLGSG ASVGRGRGAV GRKATKKTDK
110 120 130 140 150
PRLEDKDDLD VTELSNEELL DQLVRYGVNP GPIVGTTRKL YEKKLLKLRE
160 170 180 190 200
QGTESRSSTP LPTVSSSAEN TRQNGSNDSD RYSDNDEDSK IELKLEKREP
210 220 230 240 250
LKGRAKTPVT LKQRRTEHNQ SYSQAGVTET EWTSGSSTGG PLQALTREST
260 270 280 290 300
RGSRRTPRKR VETSQHFRID GAVISESTPI AETIKASSNE SLVANRLTGN
310 320 330 340 350
FKHASSILPI TEFSDITRRT PKKPLTRAEV GEKTEERRVD RDILKEMFPY
360 370 380 390 400
EASTPTGISA SCRRPIKGAA GRPLELSDFR MEESFSSKYV PKYAPLADVK
410 420 430 440 450
SEKTKKRRSV PMWIKMLLFA LVAVFLFLVY QAMETNQGNP FTNFLQDTKI

SN
Length:452
Mass (Da):50,373
Last modified:July 27, 2011 - v4
Checksum:i132AF11B458DCFBA
GO
Isoform Alpha (identifier: Q61033-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q61033.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:693
Mass (Da):75,168
GO
Isoform Zeta (identifier: Q61033-2) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q61033.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:225
Mass (Da):24,990
GO
Isoform Delta (identifier: Q61029-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     221-292: Missing.

Show »
Length:380
Mass (Da):42,650
Checksum:iF2BE6B5556030A49
GO
Isoform Epsilon (identifier: Q61029-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     221-260: Missing.

Show »
Length:412
Mass (Da):46,050
Checksum:iC54639DAB8788CEE
GO
Isoform Gamma (identifier: Q61029-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     221-329: Missing.

Show »
Length:343
Mass (Da):38,502
Checksum:iBA62B6D869B0FDAD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72 – 73EP → DA in AAC52574 (PubMed:8743987).Curated2
Sequence conflicti72 – 73EP → DA in AAC52575 (PubMed:8743987).Curated2
Sequence conflicti72 – 73EP → DA in AAC52576 (PubMed:8743987).Curated2
Sequence conflicti72 – 73EP → DA in AAC52577 (PubMed:8743987).Curated2
Sequence conflicti422 – 424VAV → GAC in AAC52574 (PubMed:8743987).Curated3
Sequence conflicti422 – 424VAV → GAC in AAC52575 (PubMed:8743987).Curated3
Sequence conflicti422 – 424VAV → GAC in AAC52576 (PubMed:8743987).Curated3
Sequence conflicti422 – 424VAV → GAC in AAC52577 (PubMed:8743987).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010132221 – 329Missing in isoform Gamma. 1 PublicationAdd BLAST109
Alternative sequenceiVSP_010131221 – 292Missing in isoform Delta. 1 PublicationAdd BLAST72
Alternative sequenceiVSP_010130221 – 260Missing in isoform Epsilon. 1 PublicationAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39074 mRNA. Translation: AAC52574.1.
U39075 mRNA. Translation: AAC52575.1.
U39076 mRNA. Translation: AAC52576.1.
U39077 mRNA. Translation: AAC52577.1.
AK150305 mRNA. Translation: BAE29455.1.
AK150515 mRNA. Translation: BAE29627.1.
AK153281 mRNA. Translation: BAE31865.1.
AK165851 mRNA. Translation: BAE38413.1.
CH466539 Genomic DNA. Translation: EDL21533.1.
CCDSiCCDS36032.1. [Q61029-4]
CCDS36033.1. [Q61029-2]
CCDS36034.1. [Q61029-3]
CCDS36035.1. [Q61029-1]
RefSeqiNP_001073598.1. NM_001080129.2. [Q61029-1]
NP_001073600.1. NM_001080131.2. [Q61029-2]
NP_001073601.1. NM_001080132.2. [Q61029-4]
UniGeneiMm.159684.
Mm.439157.

Genome annotation databases

EnsembliENSMUST00000072239; ENSMUSP00000072092; ENSMUSG00000019961. [Q61029-1]
ENSMUST00000092219; ENSMUSP00000089864; ENSMUSG00000019961. [Q61029-3]
ENSMUST00000099355; ENSMUSP00000096956; ENSMUSG00000019961. [Q61029-2]
ENSMUST00000105293; ENSMUSP00000100930; ENSMUSG00000019961. [Q61029-4]
GeneIDi21917.
KEGGimmu:21917.
UCSCiuc007gtq.2. mouse. [Q61029-1]
uc007gts.2. mouse. [Q61029-2]
uc007gtt.2. mouse. [Q61029-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiLAP2B_MOUSE
AccessioniPrimary (citable) accession number: Q61029
Secondary accession number(s): Q3UCI5
, Q61030, Q61031, Q61032
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: July 27, 2011
Last modified: November 22, 2017
This is version 154 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families