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Q61024

- ASNS_MOUSE

UniProt

Q61024 - ASNS_MOUSE

Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene

Asns

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei97 – 971GlutamineBy similarity
    Binding sitei256 – 2561ATP; via carbonyl oxygenBy similarity
    Binding sitei288 – 2881ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei365 – 3651Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi363 – 3642ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. cofactor binding Source: Ensembl

    GO - Biological processi

    1. cellular response to glucose starvation Source: Ensembl
    2. cellular response to hormone stimulus Source: Ensembl
    3. glutamine metabolic process Source: UniProtKB-KW
    4. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
    5. liver development Source: Ensembl
    6. negative regulation of apoptotic process Source: Ensembl
    7. positive regulation of mitotic cell cycle Source: Ensembl
    8. response to amino acid Source: Ensembl
    9. response to follicle-stimulating hormone Source: Ensembl
    10. response to light stimulus Source: Ensembl
    11. response to mechanical stimulus Source: Ensembl
    12. response to methotrexate Source: Ensembl
    13. response to toxic substance Source: Ensembl

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_90370. ATF4 activates genes.
    UniPathwayiUPA00134; UER00195.

    Protein family/group databases

    MEROPSiC44.974.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase
    Gene namesi
    Name:Asns
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1350929. Asns.

    Pathology & Biotechi

    Disruption phenotypei

    Mice carrying a gene trap insertion in the gene express 20% of the normal level of mRNA. The hypomorphic mutant displays a number of defects that mirror ASNSD syndrome, although the phenotype is milder. Mice have structural brain abnormalities, including reduced cortical thickness and enlarged ventricles. Mutant mice also show deficits in learning and memory. Mutant mice do not show abnormal motor activity or seizure activity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 561560Asparagine synthetase [glutamine-hydrolyzing]PRO_0000056912Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei385 – 3851N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ61024.
    PaxDbiQ61024.
    PRIDEiQ61024.

    PTM databases

    PhosphoSiteiQ61024.

    Expressioni

    Gene expression databases

    ArrayExpressiQ61024.
    BgeeiQ61024.
    CleanExiMM_ASNS.
    GenevestigatoriQ61024.

    Interactioni

    Protein-protein interaction databases

    BioGridi205108. 1 interaction.
    IntActiQ61024. 1 interaction.
    MINTiMINT-4088470.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61024.
    SMRiQ61024. Positions 76-481.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 191190Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini213 – 536324Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni49 – 535Glutamine bindingBy similarity
    Regioni75 – 773Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0367.
    GeneTreeiENSGT00390000001994.
    HOGENOMiHOG000027493.
    HOVERGENiHBG003103.
    InParanoidiQ61024.
    KOiK01953.
    OMAiWSGIYSS.
    OrthoDBiEOG75MVVS.
    PhylomeDBiQ61024.
    TreeFamiTF300603.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q61024-1 [UniParc]FASTAAdd to Basket

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    MCGIWALFGS DDCLSVQCLS AMKIAHRGPD AFRFENVNGY TNCCFGFHRL    50
    AVVDPLFGMQ PIRVRKYPYL WLCYNGEIYN HKALQQRFEF EYQTNVDGEI 100
    ILHLYDKGGI EKTICMLDGV FAFILLDTAN KKVFLGRDTY GVRPLFKAMT 150
    EDGFLAVCSE AKGLVSLKHS TTPFLKVEPF LPGHYEVLDL KPNGKVASVE 200
    MVKYHHCTDE PLHAIYDSVE KLFPGFDLET VKNNLRILFD NAIKKRLMTD 250
    RRIGCLLSGG LDSSLVAASL LKQLKEAQVQ YPLQTFAIGM EDSPDLLAAR 300
    KVANYIGSEH HEVLFNSEEG IQALDEVIFS LETYDITTVR ASVGMYLISK 350
    YIRKNTDSVV IFSGEGSDEL TQGYIYFHKA PSPEKAEEES ERLLKELYLF 400
    DVLRADRTTA AHGLELRVPF LDHRFSSYYL SLPPDMRIPK NGIEKHLLRE 450
    TFEDCNLLPK EILWRPKEAF SDGITSVKNS WFKILQDYVE HQVDDEMMSA 500
    ASQKFPFNTP KTKEGYFYRQ IFERHYPGRA DWLTHYWMPK WINATDPSAR 550
    TLTHYKSAAK A 561
    Length:561
    Mass (Da):64,283
    Last modified:January 23, 2007 - v3
    Checksum:i1981956B69E1F9F1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51W → G in BAC36254. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U38940 mRNA. Translation: AAA85125.1.
    AK076207 mRNA. Translation: BAC36254.1.
    AK167524 mRNA. Translation: BAE39594.1.
    BC005552 mRNA. Translation: AAH05552.1.
    CCDSiCCDS19907.1.
    RefSeqiNP_036185.1. NM_012055.3.
    XP_006505155.1. XM_006505092.1.
    XP_006505156.1. XM_006505093.1.
    UniGeneiMm.2942.

    Genome annotation databases

    EnsembliENSMUST00000031766; ENSMUSP00000031766; ENSMUSG00000029752.
    ENSMUST00000115542; ENSMUSP00000111204; ENSMUSG00000029752.
    GeneIDi27053.
    KEGGimmu:27053.
    UCSCiuc009axf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U38940 mRNA. Translation: AAA85125.1 .
    AK076207 mRNA. Translation: BAC36254.1 .
    AK167524 mRNA. Translation: BAE39594.1 .
    BC005552 mRNA. Translation: AAH05552.1 .
    CCDSi CCDS19907.1.
    RefSeqi NP_036185.1. NM_012055.3.
    XP_006505155.1. XM_006505092.1.
    XP_006505156.1. XM_006505093.1.
    UniGenei Mm.2942.

    3D structure databases

    ProteinModelPortali Q61024.
    SMRi Q61024. Positions 76-481.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 205108. 1 interaction.
    IntActi Q61024. 1 interaction.
    MINTi MINT-4088470.

    Protein family/group databases

    MEROPSi C44.974.

    PTM databases

    PhosphoSitei Q61024.

    Proteomic databases

    MaxQBi Q61024.
    PaxDbi Q61024.
    PRIDEi Q61024.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031766 ; ENSMUSP00000031766 ; ENSMUSG00000029752 .
    ENSMUST00000115542 ; ENSMUSP00000111204 ; ENSMUSG00000029752 .
    GeneIDi 27053.
    KEGGi mmu:27053.
    UCSCi uc009axf.1. mouse.

    Organism-specific databases

    CTDi 440.
    MGIi MGI:1350929. Asns.

    Phylogenomic databases

    eggNOGi COG0367.
    GeneTreei ENSGT00390000001994.
    HOGENOMi HOG000027493.
    HOVERGENi HBG003103.
    InParanoidi Q61024.
    KOi K01953.
    OMAi WSGIYSS.
    OrthoDBi EOG75MVVS.
    PhylomeDBi Q61024.
    TreeFami TF300603.

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .
    Reactomei REACT_90370. ATF4 activates genes.

    Miscellaneous databases

    NextBioi 304991.
    PROi Q61024.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61024.
    Bgeei Q61024.
    CleanExi MM_ASNS.
    Genevestigatori Q61024.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Goodwin L., Sellati L., Millan C., Guzowski D., Leeds N., Broome J., Pergolizzi R.
      Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Liver.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Placenta.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    4. Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiASNS_MOUSE
    AccessioniPrimary (citable) accession number: Q61024
    Secondary accession number(s): Q3TJA1, Q8BPC8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3