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Protein

E3 ubiquitin-protein ligase DTX1

Gene

Dtx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1. Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri418 – 47962RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • glial cell differentiation Source: MGI
  • negative regulation of neuron differentiation Source: MGI
  • negative regulation of T cell differentiation Source: MGI
  • Notch signaling pathway Source: MGI
  • protein ubiquitination Source: UniProtKB-UniPathway
  • regulation of Notch signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Notch signaling pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase DTX1 (EC:6.3.2.-)
Alternative name(s):
FXI-T1
Protein deltex-1
Short name:
Deltex1
Short name:
mDTX1
Gene namesi
Name:Dtx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1352744. Dtx1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Predominantly cytoplasmic. Associates with endocytic vesicles. Partially nuclear.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 627627E3 ubiquitin-protein ligase DTX1PRO_0000219081Add
BLAST

Post-translational modificationi

Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ61010.
MaxQBiQ61010.
PaxDbiQ61010.
PRIDEiQ61010.

PTM databases

PhosphoSiteiQ61010.

Expressioni

Tissue specificityi

Predominantly expressed in the brain and testis. Weakly expressed in the thymus, spleen and ovary. Predominantly expressed in regions containing post-mitotic differentiating neurons.2 Publications

Developmental stagei

In the CNS, it is expressed in the developing neural tube starting from E10.5 in the spinal cord and around E11.5 in the telencephalon. Expressed ubiquitously throughout the spinal cord and telencephalon during neurogenesis. Expressed throughout the developing retina from E12.5 to E15.5. Expressed in the developing thymus. Not expressed in the somite or presomite during somitogenesis. Expressed slightly later that Dtx2.2 Publications

Gene expression databases

BgeeiQ61010.
CleanExiMM_DTX1.
GenevisibleiQ61010. MM.

Interactioni

Subunit structurei

Homodimer. May form a heterodimer with other members of the Deltex family. Interacts with NOTCH1 via its N-terminal region and EIF3F, the interaction is required for NOTCH1 deubiquitination. Interacts with EP300. Forms a heterodimer with BBAP; the heterodimerization leading to an increase of in vitro ubiquitin ligase activity. Interacts with ITCH (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199771. 2 interactions.
STRINGi10090.ENSMUSP00000031607.

Structurei

3D structure databases

ProteinModelPortaliQ61010.
SMRiQ61010. Positions 25-179, 396-620.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 9481WWE 1PROSITE-ProRule annotationAdd
BLAST
Domaini95 – 17177WWE 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi240 – 2434SH3-bindingSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi227 – 382156Pro-richAdd
BLAST

Domaini

The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.By similarity

Sequence similaritiesi

Belongs to the Deltex family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 2 WWE domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri418 – 47962RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, SH3-binding, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IEMS. Eukaryota.
ENOG4111EY2. LUCA.
GeneTreeiENSGT00440000035370.
HOVERGENiHBG007213.
InParanoidiQ61010.
KOiK06058.
OMAiYSAAVCH.
OrthoDBiEOG7J17ZB.
TreeFamiTF325526.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02825. WWE. 2 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00678. WWE. 2 hits.
[Graphical view]
PROSITEiPS50918. WWE. 2 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61010-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRPGQGVMV PVNGLGFPPQ NVARVVVWEW LNEHSRWRPY TATVCHHIEN
60 70 80 90 100
VLKEDARGSV VLGQVDAQLV PYIIDLQSMH QFRQDTGTMR PVRRNFYDPS
110 120 130 140 150
SAPGKGIVWE WENDGGAWTA YDMDICITIQ NAYEKQHPWL DLSSLGFCYL
160 170 180 190 200
IYFNSMSQMN RQTRRRRRLR RRLDLAYPLT VGSIPKSQSW PVGASSGQPC
210 220 230 240 250
SCQQCLLVNS TRAASNAILA SQRRKAPIAP AAPPAPPPPP PPLPPGGPPG
260 270 280 290 300
ALVVRPSATF AGAALWAAPA TGPTEPAPPP GVPPRSPSAP NGAPTPGQNN
310 320 330 340 350
LSRPGPQRST SVSARASIPP GVPALPVKNL NGTGPVHPAL AGMTGILLCA
360 370 380 390 400
AGLPVCLTRA PKPILHPPPV SKSDVKPVPG VPGVCRKTKK KHLKKSKNPE
410 420 430 440 450
DVVRRYMQKV KNPPDEDCTI CMERLVTASG YEGVLRNKSV RPELVGRLGR
460 470 480 490 500
CGHMYHLLCL VAMYSNGNKD GSLQCPTCKA IYGEKTGTQP PGKMEFHLIP
510 520 530 540 550
HSLPGFADTQ TIRIVYDIPT GIQGPEHPNP GKKFTARGFP RHCYLPNNEK
560 570 580 590 600
GRKVLRLLIT AWERRLIFTI GTSNTTGESD TVVWNEIHHK TEFGSNLTGH
610 620
GYPDASYLDN VLAELTAQGV SEAMAKA
Length:627
Mass (Da):68,119
Last modified:September 26, 2003 - v2
Checksum:i6ED8C152EF5A55FD
GO
Isoform 2 (identifier: Q61010-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Note: Splicing acceptor site not canonical. No experimental confirmation available.
Show »
Length:549
Mass (Da):59,375
Checksum:i68D766D4069971DB
GO
Isoform 3 (identifier: Q61010-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-88: Missing.

Note: No experimental confirmation available.
Show »
Length:539
Mass (Da):58,173
Checksum:i107E052154DF2DCC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti451 – 4511C → Y in BAC40465 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8888Missing in isoform 3. 1 PublicationVSP_008349Add
BLAST
Alternative sequencei1 – 7878Missing in isoform 2. 1 PublicationVSP_008348Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38252 mRNA. Translation: AAB02905.1.
AB015422 mRNA. Translation: BAB18939.1.
AK088630 mRNA. Translation: BAC40465.1.
AK169455 mRNA. Translation: BAE41183.1.
BC053055 mRNA. Translation: AAH53055.1.
CCDSiCCDS19624.1. [Q61010-1]
RefSeqiNP_032078.2. NM_008052.3. [Q61010-1]
UniGeneiMm.1645.

Genome annotation databases

EnsembliENSMUST00000031607; ENSMUSP00000031607; ENSMUSG00000029603. [Q61010-1]
GeneIDi14357.
KEGGimmu:14357.
UCSCiuc008zht.1. mouse. [Q61010-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38252 mRNA. Translation: AAB02905.1.
AB015422 mRNA. Translation: BAB18939.1.
AK088630 mRNA. Translation: BAC40465.1.
AK169455 mRNA. Translation: BAE41183.1.
BC053055 mRNA. Translation: AAH53055.1.
CCDSiCCDS19624.1. [Q61010-1]
RefSeqiNP_032078.2. NM_008052.3. [Q61010-1]
UniGeneiMm.1645.

3D structure databases

ProteinModelPortaliQ61010.
SMRiQ61010. Positions 25-179, 396-620.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199771. 2 interactions.
STRINGi10090.ENSMUSP00000031607.

PTM databases

PhosphoSiteiQ61010.

Proteomic databases

EPDiQ61010.
MaxQBiQ61010.
PaxDbiQ61010.
PRIDEiQ61010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031607; ENSMUSP00000031607; ENSMUSG00000029603. [Q61010-1]
GeneIDi14357.
KEGGimmu:14357.
UCSCiuc008zht.1. mouse. [Q61010-1]

Organism-specific databases

CTDi1840.
MGIiMGI:1352744. Dtx1.

Phylogenomic databases

eggNOGiENOG410IEMS. Eukaryota.
ENOG4111EY2. LUCA.
GeneTreeiENSGT00440000035370.
HOVERGENiHBG007213.
InParanoidiQ61010.
KOiK06058.
OMAiYSAAVCH.
OrthoDBiEOG7J17ZB.
TreeFamiTF325526.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.

Miscellaneous databases

NextBioi285813.
PROiQ61010.
SOURCEiSearch...

Gene expression databases

BgeeiQ61010.
CleanExiMM_DTX1.
GenevisibleiQ61010. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02825. WWE. 2 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00678. WWE. 2 hits.
[Graphical view]
PROSITEiPS50918. WWE. 2 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel cDNA transcript expressed in fractionated X-irradiation-induced murine thymomas."
    Pampeno C.L., Meruelo D.
    Cell Growth Differ. 7:1113-1123(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
  2. "Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis."
    Kishi N., Tang Z., Maeda Y., Hirai A., Mo R., Ito M., Suzuki S., Nakao K., Kinoshita T., Kadesch T., Hui C.-C., Artavanis-Tsakonas S., Okano H., Matsuno K.
    Int. J. Dev. Neurosci. 19:21-35(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MULTIMERIZATION, INTERACTION WITH NOTCH1.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J and NOD.
    Tissue: Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  5. "Expression of Deltex1 during mouse embryogenesis: comparison with Notch1, 2 and 3 expression."
    Mitsiadis T.A., Gayet O., Zhang N., Carroll P.
    Mech. Dev. 109:399-403(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  6. "Deltex regulates T-cell activation by targeted degradation of active MEKK1."
    Liu W.H., Lai M.Z.
    Mol. Cell. Biol. 25:1367-1378(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN E3 LIGASE.

Entry informationi

Entry nameiDTX1_MOUSE
AccessioniPrimary (citable) accession number: Q61010
Secondary accession number(s): Q3TER5, Q8C2H2, Q9ER09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: September 26, 2003
Last modified: March 16, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.