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Protein

Scavenger receptor class B member 1

Gene

Scarb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells (By similarity). Both isoform 1 and isoform 2 act as receptors for HDL, mediating selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux (PubMed:9254074, PubMed:9614139). Also facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. May be involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity (By similarity).By similarity2 Publications

GO - Molecular functioni

  • amyloid-beta binding Source: ARUK-UCL
  • apolipoprotein A-I binding Source: MGI
  • apolipoprotein binding Source: BHF-UCL
  • high-density lipoprotein particle binding Source: BHF-UCL
  • high-density lipoprotein particle receptor activity Source: BHF-UCL
  • lipopolysaccharide binding Source: MGI
  • lipopolysaccharide receptor activity Source: MGI
  • low-density lipoprotein particle binding Source: BHF-UCL
  • phosphatidylserine binding Source: Ensembl
  • protein homodimerization activity Source: Ensembl

GO - Biological processi

  • adhesion of symbiont to host Source: MGI
  • androgen biosynthetic process Source: Ensembl
  • blood vessel endothelial cell migration Source: MGI
  • cholesterol catabolic process Source: MGI
  • cholesterol efflux Source: BHF-UCL
  • cholesterol homeostasis Source: BHF-UCL
  • cholesterol import Source: BHF-UCL
  • cholesterol transport Source: MGI
  • detection of lipopolysaccharide Source: MGI
  • endothelial cell proliferation Source: MGI
  • high-density lipoprotein particle clearance Source: BHF-UCL
  • high-density lipoprotein particle remodeling Source: BHF-UCL
  • intestinal absorption Source: MGI
  • lipopolysaccharide transport Source: MGI
  • low-density lipoprotein particle clearance Source: BHF-UCL
  • phospholipid transport Source: BHF-UCL
  • positive regulation of cholesterol storage Source: BHF-UCL
  • positive regulation of nitric-oxide synthase activity Source: MGI
  • positive regulation of triglyceride biosynthetic process Source: BHF-UCL
  • recognition of apoptotic cell Source: BHF-UCL
  • regulation of phagocytosis Source: Ensembl
  • regulation of phosphatidylcholine catabolic process Source: BHF-UCL
  • reverse cholesterol transport Source: BHF-UCL
  • triglyceride homeostasis Source: BHF-UCL
  • vitamin transmembrane transport Source: MGI

Keywordsi

Molecular functionReceptor

Enzyme and pathway databases

ReactomeiR-MMU-3000471. Scavenging by Class B Receptors.
R-MMU-8964011. HDL clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Scavenger receptor class B member 1
Short name:
SRB1
Alternative name(s):
SR-BI
Gene namesi
Name:Scarb1
Synonyms:Srb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:893578. Scarb1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Topological domaini33 – 440ExtracellularSequence analysisAdd BLAST408
Transmembranei441 – 461HelicalSequence analysisAdd BLAST21
Topological domaini462 – 509CytoplasmicSequence analysisAdd BLAST48

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1741203.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001441611 – 509Scavenger receptor class B member 1Add BLAST509

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi102N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi108N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi116N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi173N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi212N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi227N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi251 ↔ 3841 Publication
Glycosylationi255N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi288N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi310N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi330N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi383N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi462S-palmitoyl cysteine1 Publication1

Post-translational modificationi

N-glycosylated.By similarity
The six cysteines of the extracellular domain are all involved in intramolecular disulfide bonds.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

EPDiQ61009.
MaxQBiQ61009.
PaxDbiQ61009.
PeptideAtlasiQ3TZ42.
PRIDEiQ61009.

PTM databases

iPTMnetiQ61009.
PhosphoSitePlusiQ61009.
SwissPalmiQ61009.

Expressioni

Tissue specificityi

Expressed primarily in liver, ovary and adrenal gland, and, at lower levels in other non-placental steroidogenic tissues, including adipose tissue, mammary gland and testis (at protein level) (PubMed:8560269, PubMed:9254074, PubMed:9614139). Isoform 2 is expressed at lower levels than isoform 1 in liver, testis and adrenal gland (PubMed:9614139). At the mRNA, but not at the protein level, isoform 2 is the predominant isoform in testis (80%) (PubMed:9254074).3 Publications

Inductioni

Both isoform 1 and isoform 2 are up-regulated in response to adrenocorticotropic hormone (ACTH).1 Publication

Gene expression databases

BgeeiENSMUSG00000037936.
CleanExiMM_SCARB1.
ExpressionAtlasiQ61009. baseline and differential.
GenevisibleiQ61009. MM.

Interactioni

Subunit structurei

The C-terminal region binds to PDZK1.By similarity

GO - Molecular functioni

  • apolipoprotein A-I binding Source: MGI
  • apolipoprotein binding Source: BHF-UCL
  • protein homodimerization activity Source: Ensembl

Protein-protein interaction databases

MINTiMINT-242627.
STRINGi10090.ENSMUSP00000083242.

Chemistry databases

BindingDBiQ61009.

Structurei

Secondary structure

1509
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi407 – 417Combined sources11
Turni418 – 422Combined sources5
Helixi427 – 434Combined sources8
Helixi440 – 455Combined sources16
Helixi457 – 468Combined sources12
Beta strandi471 – 473Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3R69X-ray1.50A/B505-509[»]
5KTFNMR-A405-475[»]
ProteinModelPortaliQ61009.
SMRiQ61009.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CD36 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3776. Eukaryota.
ENOG410XS17. LUCA.
GeneTreeiENSGT00530000062927.
HOGENOMiHOG000252951.
HOVERGENiHBG106577.
InParanoidiQ61009.
KOiK13885.
OMAiPDACRSM.
OrthoDBiEOG091G0CH9.
PhylomeDBiQ61009.
TreeFamiTF317925.

Family and domain databases

InterProiView protein in InterPro
IPR005428. CD36/SCARB1/SNMP1.
IPR002159. CD36_fam.
PANTHERiPTHR11923. PTHR11923. 1 hit.
PfamiView protein in Pfam
PF01130. CD36. 1 hit.
PRINTSiPR01610. CD36ANTIGEN.
PR01609. CD36FAMILY.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61009-1) [UniParc]FASTAAdd to basket
Also known as: SR-BI1 Publication, SR-BI.11 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGGSSRARWV ALGLGALGLL FAALGVVMIL MVPSLIKQQV LKNVRIDPSS
60 70 80 90 100
LSFGMWKEIP VPFYLSVYFF EVVNPNEVLN GQKPVVRERG PYVYREFRQK
110 120 130 140 150
VNITFNDNDT VSFVENRSLH FQPDKSHGSE SDYIVLPNIL VLGGSILMES
160 170 180 190 200
KPVSLKLMMT LALVTMGQRA FMNRTVGEIL WGYDDPFVHF LNTYLPDMLP
210 220 230 240 250
IKGKFGLFVG MNNSNSGVFT VFTGVQNFSR IHLVDKWNGL SKIDYWHSEQ
260 270 280 290 300
CNMINGTSGQ MWAPFMTPES SLEFFSPEAC RSMKLTYNES RVFEGIPTYR
310 320 330 340 350
FTAPDTLFAN GSVYPPNEGF CPCRESGIQN VSTCRFGAPL FLSHPHFYNA
360 370 380 390 400
DPVLSEAVLG LNPNPKEHSL FLDIHPVTGI PMNCSVKMQL SLYIKSVKGI
410 420 430 440 450
GQTGKIEPVV LPLLWFEQSG AMGGKPLSTF YTQLVLMPQV LHYAQYVLLG
460 470 480 490 500
LGGLLLLVPI ICQLRSQEKC FLFWSGSKKG SQDKEAIQAY SESLMSPAAK

GTVLQEAKL
Length:509
Mass (Da):56,754
Last modified:November 1, 1996 - v1
Checksum:i5CFCDD62DD6ECB1C
GO
Isoform 2 (identifier: Q61009-2) [UniParc]FASTAAdd to basket
Also known as: SR-BI.21 Publication, SR-BII1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     468-509: EKCFLFWSGS...KGTVLQEAKL → GPEDTISPPN...LSGQPTSAMA

Show »
Length:506
Mass (Da):56,242
Checksum:i59B6D5E040413288
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti235D → G in BAE34368 (PubMed:16141072).Curated1
Sequence conflicti396S → F in BAB27068 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058986468 – 509EKCFL…QEAKL → GPEDTISPPNLIAWSDQPPS PYTPLLEDSLSGQPTSAMA in isoform 2. Add BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37799 mRNA. Translation: AAC52378.1.
AK010622 mRNA. Translation: BAB27068.1.
AK028191 mRNA. Translation: BAC25802.1.
AK033114 mRNA. Translation: BAC28157.1.
AK154933 mRNA. Translation: BAE32934.1.
AK158122 mRNA. Translation: BAE34368.1.
AK169906 mRNA. Translation: BAE41449.1.
AC162802 Genomic DNA. No translation available.
CT010222 mRNA. Translation: CAJ18430.1.
CH466529 Genomic DNA. Translation: EDL19562.1.
CH466529 Genomic DNA. Translation: EDL19563.1.
BC004656 mRNA. Translation: AAH04656.1.
CCDSiCCDS19683.1. [Q61009-1]
CCDS57383.1. [Q61009-2]
RefSeqiNP_001192011.1. NM_001205082.1. [Q61009-2]
NP_058021.1. NM_016741.2. [Q61009-1]
UniGeneiMm.282242.
Mm.474018.

Genome annotation databases

EnsembliENSMUST00000086075; ENSMUSP00000083242; ENSMUSG00000037936. [Q61009-1]
ENSMUST00000111390; ENSMUSP00000107021; ENSMUSG00000037936. [Q61009-2]
GeneIDi20778.
KEGGimmu:20778.
UCSCiuc008zrd.2. mouse. [Q61009-1]
uc008zre.2. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSCRB1_MOUSE
AccessioniPrimary (citable) accession number: Q61009
Secondary accession number(s): D3Z2V4
, Q3TZ42, Q4FK30, Q9CWJ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families