Reviewed, UniProtKB/Swiss-Prot Q60WT2 (ACASE_CAEBR)

Names and origin

Protein names

Recommended name:
    Alkaline ceramidase
      Short name=AlkCDase
    EC=3.5.1.23
Alternative name(s):
    Alkaline N-acylsphingosine amidohydrolase
    Alkaline acylsphingosine deacylase

Gene names

ORF Names:

CBG18997

Organism

Caenorhabditis briggsae [Complete proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid By similarity.
Catalytic activity	N-acylsphingosine + H2O = a carboxylate + sphingosine.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the alkaline ceramidase family.

Ontologies

Keywords
Biological process	Lipid metabolism
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ceramide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: InterPro endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Alkaline ceramidase		PRO_0000247750
Regions
	Transmembrane	34 – 54	21	Potential
	Transmembrane	61 – 81	21	Potential
	Transmembrane	96 – 116	21	Potential
	Transmembrane	124 – 144	21	Potential
	Transmembrane	148 – 168	21	Potential
	Transmembrane	183 – 203	21	Potential
	Transmembrane	214 – 234	21	Potential
Amino acid modifications
	Glycosylation	256	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q60WT2-1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: CD1DB0CFE92258B7	FASTA	272	31,262
	10 20 30 40 50 60 MDSSSISRWF EYESGHAWCE SAYKYQTLPY VAEFANTCTN LPIIVLPLVN IMLLRRYLHD 70 80 90 100 110 120 VNGGLVFPQL LLTFNGLAST YYHATLNLFG QLVDELSLVW IITVFLVVYI PVMKWFPERF 130 140 150 160 170 180 SKRLTVVRWV VLIVTAVVSA LCFLEPNLNA IALMLFSIPA AVVIRYEGKQ SGIPDIENFP 190 200 210 220 230 240 SRILALWGVA FSFWFADRLL CDFWLYLGTP YLHALFHLLA GLAGYTIFIM FSMIDIESRS 250 260 270 KTHRYTAAVR YFPDKNGSIF SFPYISLKER SQ

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed: 14624247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CAAC02000558 Genomic DNA. Translation: CAP36316.2.
3D structure databases
ModBase	Search...
Organism-specific databases
WormBase	WBGene00038287. CBG18997.
Enzyme and pathway databases
BRENDA	3.5.1.23. 261794.
Family and domain databases
InterPro	IPR008901. APHC. [Graphical view]
Pfam	PF05875. aPHC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACASE_CAEBR

Accession

Primary (citable) accession number: Q60WT2
Secondary accession number(s): A8XUJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	October 13, 2009
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project