Reviewed, UniProtKB/Swiss-Prot Q60V90 (NDST_CAEBR)

Names and origin

Protein names

Recommended name:
    Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
    EC=2.8.2.8
Alternative name(s):
    Glucosaminyl N-deacetylase/N-sulfotransferase 1
Including the following 2 domains:
    1- Recommended name:
            Heparan sulfate N-deacetylase 1
              EC=3.-.-.-
    2- Recommended name:
            Heparan sulfate N-sulfotransferase 1
              EC=2.8.2.-

Gene names

Name:	hst-1
ORF Names:	CBG19653

Organism

Caenorhabditis briggsae [Complete proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis By similarity.
Catalytic activity	3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine.
Pathway	Glycan metabolism; heparan sulfate biosynthesis. Glycan metabolism; heparin biosynthesis.
Subunit structure	Monomer By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the sulfotransferase 1 family. NDST subfamily.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	[heparan sulfate]-glucosamine N-sulfotransferase activity Inferred from electronic annotation. Source: EC hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 859	859	Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1		PRO_0000225663
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 34	21	Signal-anchor for type II membrane protein Potential
	Topological domain	35 – 859	825	Lumenal Potential
	Nucleotide binding	593 – 597	5	PAPS By similarity
	Nucleotide binding	810 – 814	5	PAPS By similarity
	Region	34 – 575	542	Heparan sulfate N-deacetylase 1
	Region	576 – 859	284	Heparan sulfate N-sulfotransferase 1
Sites
	Active site	593	1	For sulfotransferase activity By similarity
	Binding site	687	1	PAPS By similarity
Amino acid modifications
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
	Glycosylation	74	1	N-linked (GlcNAc...) Potential
	Glycosylation	210	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential
	Glycosylation	378	1	N-linked (GlcNAc...) Potential
	Glycosylation	429	1	N-linked (GlcNAc...) Potential
	Glycosylation	608	1	N-linked (GlcNAc...) Potential
	Glycosylation	643	1	N-linked (GlcNAc...) Potential
	Glycosylation	715	1	N-linked (GlcNAc...) Potential
	Disulfide bond	796 ↔ 805		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60V90-1 [UniParc]. Last modified September 22, 2009. Version 3. Checksum: 56D9CD5DA5B886F2	FASTA	859	100,178
	10 20 30 40 50 60 MIITPYLNPR LVKPLKWLAI IILLYFLYFS LFSINKKPGK PRKPPKAVEN YTCPFEKADF 70 80 90 100 110 120 SNFKDKKLEF HKNNGTDPKI LVILDSLFSR HGKSIIQILN SQKFAFKAEA ISKNLPVLTS 130 140 150 160 170 180 AKRGKYSLII VENYYKYLNM ARWNRQLLDK YCKEYRVPLF SFIASKPNDQ LKRIRIKGSS 190 200 210 220 230 240 LWMWQNQRIN RLTVSPSPIH KISKIGAYRN LTTQESDWIL FEISENFESI LTGTVKNGYE 250 260 270 280 290 300 RAVVLRDLGR EDGVEKVIFG RNLTDFQIKI TFLDALWWAM GDEKLFGLDR FVQVDIDDVF 310 320 330 340 350 360 VGAQSTRIVE EDVRHLISAQ NHFRNFIENF KFLLGFSGSY FRNGDDFEDR GDEILIENAE 370 380 390 400 410 420 KFVWFPHMWR HNHAHEHNFT YLESIMVQNR LFAQNMHLPI DYPYAIAPQH DGVFPVHEQM 430 440 450 460 470 480 YEAWKKIWNV TVTATEEYPH LKPATGRKGF IHSGIHVLPR QTCGLYTHTQ FFDEYPEGFQ 490 500 510 520 530 540 KVIKSIQGGD LFFTILLNPI SIFMTHQQNY AHDRLALYTF ENLFRFLNCW TNIRLKWQSP 550 560 570 580 590 600 VESAKMYFEK FPEERIPLWT NPCSDPRHQA ILPPSMSCSK KSLPDLLIIG PQKTGSTALA 610 620 630 640 650 660 SFLALHPNVS QNMEIPGSFE EIQFFSGQNY LKGVEWYMSK FPNETTVIFE KSATYFDNPS 670 680 690 700 710 720 AARQAAAMVP HAKLVIILQN PTQRAYSWFQ SLFQHLIAHK DPIAMSSESL DVILNSTSSE 730 740 750 760 770 780 SAKFKIRQRC LSGGRYVHHL DKWLEHFSLQ QIQFIDSDEL RKEPAKVLSS LSKWLDLPEF 790 800 810 820 830 840 PFETHIRFSP SKGFHCRLIN GKTECLGESK GRKYSEMSQE LRQKLDGIFA LDNSALFKFL 850 RKNRLKIPDW LEEAVRIRV

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed: 14624247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CAAC02000561 Genomic DNA. Translation: CAP36860.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus hst-1 in contig CU538967_GR.
UCSC	F08B4.6. c. elegans.
Organism-specific databases
WormBase	WBGene00038831. Cbr-hst-1.
Enzyme and pathway databases
BRENDA	2.8.2.8. 261794.
Family and domain databases
InterPro	IPR000863. Sulfotransferase. [Graphical view]
Pfam	PF00685. Sulfotransfer_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDST_CAEBR

Accession

Primary (citable) accession number: Q60V90
Secondary accession number(s): A8XW41

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 22, 2009
Last modified:	November 3, 2009
This is version 34 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project