Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 - Caenorhabditis briggsae

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Q60V90 (NDST_CAEBR) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 46. History...

Clusters with 100%, 90%, 50% identity |

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Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
EC=2.8.2.8

Alternative name(s):
Glucosaminyl N-deacetylase/N-sulfotransferase 1

Including the following 2 domains:

Heparan sulfate N-deacetylase 1
EC=3.-.-.-
Heparan sulfate N-sulfotransferase 1
EC=2.8.2.-

Gene names

Name:	hst-1
ORF Names:	CBG19653

Organism

Caenorhabditis briggsae

Taxonomic identifier

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis By similarity.
Catalytic activity	3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine.
Pathway	Glycan metabolism; heparan sulfate biosynthesis. Glycan metabolism; heparin biosynthesis.
Subunit structure	Monomer By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the sulfotransferase 1 family. NDST subfamily.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	[heparan sulfate]-glucosamine N-sulfotransferase activity Inferred from electronic annotation. Source: EC hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

859

Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1

PRO_0000225663

Regions

Topological domain

13

Cytoplasmic Potential

Transmembrane

21

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

825

Lumenal Potential

Nucleotide binding

5

PAPS By similarity

Nucleotide binding

5

PAPS By similarity

Region

542

Heparan sulfate N-deacetylase 1

Region

284

Heparan sulfate N-sulfotransferase 1

Sites

Active site

1

For sulfotransferase activity By similarity

Binding site

1

PAPS By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Disulfide bond

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q60V90 [UniParc].

Last modified September 22, 2009. Version 3.
Checksum: 56D9CD5DA5B886F2
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859

100,178

        10         20         30         40         50         60 
MIITPYLNPR LVKPLKWLAI IILLYFLYFS LFSINKKPGK PRKPPKAVEN YTCPFEKADF 

        70         80         90        100        110        120 
SNFKDKKLEF HKNNGTDPKI LVILDSLFSR HGKSIIQILN SQKFAFKAEA ISKNLPVLTS 

       130        140        150        160        170        180 
AKRGKYSLII VENYYKYLNM ARWNRQLLDK YCKEYRVPLF SFIASKPNDQ LKRIRIKGSS 

       190        200        210        220        230        240 
LWMWQNQRIN RLTVSPSPIH KISKIGAYRN LTTQESDWIL FEISENFESI LTGTVKNGYE 

       250        260        270        280        290        300 
RAVVLRDLGR EDGVEKVIFG RNLTDFQIKI TFLDALWWAM GDEKLFGLDR FVQVDIDDVF 

       310        320        330        340        350        360 
VGAQSTRIVE EDVRHLISAQ NHFRNFIENF KFLLGFSGSY FRNGDDFEDR GDEILIENAE 

       370        380        390        400        410        420 
KFVWFPHMWR HNHAHEHNFT YLESIMVQNR LFAQNMHLPI DYPYAIAPQH DGVFPVHEQM 

       430        440        450        460        470        480 
YEAWKKIWNV TVTATEEYPH LKPATGRKGF IHSGIHVLPR QTCGLYTHTQ FFDEYPEGFQ 

       490        500        510        520        530        540 
KVIKSIQGGD LFFTILLNPI SIFMTHQQNY AHDRLALYTF ENLFRFLNCW TNIRLKWQSP 

       550        560        570        580        590        600 
VESAKMYFEK FPEERIPLWT NPCSDPRHQA ILPPSMSCSK KSLPDLLIIG PQKTGSTALA 

       610        620        630        640        650        660 
SFLALHPNVS QNMEIPGSFE EIQFFSGQNY LKGVEWYMSK FPNETTVIFE KSATYFDNPS 

       670        680        690        700        710        720 
AARQAAAMVP HAKLVIILQN PTQRAYSWFQ SLFQHLIAHK DPIAMSSESL DVILNSTSSE 

       730        740        750        760        770        780 
SAKFKIRQRC LSGGRYVHHL DKWLEHFSLQ QIQFIDSDEL RKEPAKVLSS LSKWLDLPEF 

       790        800        810        820        830        840 
PFETHIRFSP SKGFHCRLIN GKTECLGESK GRKYSEMSQE LRQKLDGIFA LDNSALFKFL 

       850 
RKNRLKIPDW LEEAVRIRV

Cross-references

Sequence databases
EMBL GenBank DDBJ	CAAC02000561 Genomic DNA. Translation: CAP36860.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q60V90.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
WormBase	CBG19653; CBP36360; WBGene00038831.
Family and domain databases
InterPro	IPR021930. Heparan_SO4_deacetylase. IPR000863. Sulfotransferase_dom. [Graphical view]
Pfam	PF12062. HSNSD. 1 hit. PF00685. Sulfotransfer_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDST_CAEBR

Accession

Primary (citable) accession number: Q60V90
Secondary accession number(s): A8XW41

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 22, 2009
Last modified:	September 21, 2011
This is version 46 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents