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Q60HH9

- HEM2_MACFA

UniProt

Q60HH9 - HEM2_MACFA

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Protein
Delta-aminolevulinic acid dehydratase
Gene
ALAD, QorA-10240
Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Binds 8 zinc ions per octamer. Requires four zinc ions per octamer for full catalytic activity. Can bind up to 2 zinc ions per subunit By similarity.

Enzyme regulationi

Can alternate between a fully active homooctamer and a low-activity homohexamer. A bound magnesium ion may promote the assembly of the fully active homooctamer. The magnesium-binding site is absent in the low-activity homohexamer. Inhibited by compounds that favor the hexameric state. Inhibited by divalent lead ions. The lead ions partially displace the zinc cofactor By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi122 – 1221Zinc 1; catalytic By similarity
Metal bindingi124 – 1241Zinc 1; catalytic By similarity
Metal bindingi131 – 1311Zinc 2 By similarity
Metal bindingi132 – 1321Zinc 1; catalytic By similarity
Active sitei199 – 1991Schiff-base intermediate with substrate By similarity
Binding sitei209 – 2091Substrate 1 By similarity
Binding sitei221 – 2211Substrate 1 By similarity
Metal bindingi223 – 2231Zinc 2 By similarity
Active sitei252 – 2521Schiff-base intermediate with substrate By similarity
Binding sitei279 – 2791Substrate 2 By similarity
Binding sitei318 – 3181Substrate 2 By similarity

GO - Molecular functioni

  1. lead ion binding Source: UniProtKB
  2. porphobilinogen synthase activity Source: UniProtKB
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. heme biosynthetic process Source: UniProtKB
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:ALAD
ORF Names:QorA-10240
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Delta-aminolevulinic acid dehydratase
PRO_0000140527Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991N6-succinyllysine By similarity
Modified residuei215 – 2151Phosphoserine By similarity
Modified residuei252 – 2521N6-succinyllysine By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homooctamer; active form. Homohexamer; low activity form By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ60HH9.
SMRiQ60HH9. Positions 1-328.

Family & Domainsi

Sequence similaritiesi

Belongs to the ALADH family.

Phylogenomic databases

HOVERGENiHBG001222.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60HH9-1 [UniParc]FASTAAdd to Basket

« Hide

MQPQSVLHSG YFHPLLRAWQ TATTTLNASN LIYPIFVTDV PDDIQPIASL    50
PGVARYGVNR LEEMLRPLVE EGLRCVLIFG IPSRVPKDER GSAADSEESP 100
AIEAIHLLRK TFPNLLVACD ICLCPYTSHG HCGLLSENGA FRAEESRQRL 150
AEVALAYAKA GCQVVAPSDM MDGRVEAIKE ALMAHGLGNR VSVMSYSAKF 200
ASCFYGPFRD AAQSSPAFGD RRCYQLPPGA RGLALRAVDR DVREGADVLM 250
VKPGMPYLDI VREVKDKHPD LPLAVYHVSG EFAMLWHGAQ AGAFDLKAAV 300
LEAMTAFRRA GADIIITYYT PQLLQWLKKE 330
Length:330
Mass (Da):36,246
Last modified:April 12, 2005 - v2
Checksum:i70527006BE58BEAA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB125148 mRNA. Translation: BAD51936.1. Sequence problems.
UniGeneiMfa.13697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB125148 mRNA. Translation: BAD51936.1 . Sequence problems.
UniGenei Mfa.13697.

3D structure databases

ProteinModelPortali Q60HH9.
SMRi Q60HH9. Positions 1-328.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG001222.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and characterization of cDNA for macaque neurological disease genes."
    Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Occipital cortex.

Entry informationi

Entry nameiHEM2_MACFA
AccessioniPrimary (citable) accession number: Q60HH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: April 12, 2005
Last modified: March 19, 2014
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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