Q60HH4 (ASAH1_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acid ceramidase
Short name=AC
Short name=ACDase
Short name=Acid CDase
EC=3.5.1.23

Alternative name(s):
Acylsphingosine deacylase
N-acylsphingosine amidohydrolase

Cleaved into the following 2 chains:

1. Acid ceramidase subunit alpha
2. Acid ceramidase subunit beta

Gene names

Name:	ASAH1
ORF Names:	QccE-14484

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid By similarity.
Catalytic activity	N-acylsphingosine + H2O = a carboxylate + sphingosine.
Subunit structure	Heterodimer of one alpha and one beta subunit By similarity.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the acid ceramidase family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 142	121	Acid ceramidase subunit alpha		PRO_0000002314
	Chain	143 – 395	253	Acid ceramidase subunit beta		PRO_0000002315
Amino acid modifications
	Glycosylation	173	1	N-linked (GlcNAc...) Potential
	Glycosylation	195	1	N-linked (GlcNAc...) Potential
	Glycosylation	259	1	N-linked (GlcNAc...) Potential
	Glycosylation	286	1	N-linked (GlcNAc...) Potential
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
	Glycosylation	348	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q60HH4 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 49AA1690945A8B67	FASTA	395	44,818
	10 20 30 40 50 60 MLGRSRLALV LLAAAVSCAV AQHAPPWTED CRKSTYPPSG PTYRGPAPWY TINLDLPPYK 70 80 90 100 110 120 RWHELMVDKA PMLKVIVNSL KNMVNTFVPS GKVMQIVDEK LPGLLGNFPG PFEEEMKGIA 130 140 150 160 170 180 AVTDIPLGEI ISYNIFYEFF TLCTSIVAED KKGHLIHGRN MDFGVFLGWN INNDTWVITE 190 200 210 220 230 240 ELKPLTVNLD FQRNNKTVFK ASSFAGYVGM LTGFKPGLFS LTLNERFSVN GGYLGILEWI 250 260 270 280 290 300 LGKKDAMWIG FLTRTVLENS TSYEEAKNIL TKTKILAPAY FILGGNQSGE GCVITRDRKE 310 320 330 340 350 360 SLDVYELDAK QGRWYVVQTN YDRWKNPFFL DDRRTPAKMC LNRTTQENIS FENMYDVLST 370 380 390 KPVLNKLTVF TTLIDVTKDQ FETYMRDCPD PCIGW

References

[1]	"Isolation and characterization of cDNA for macaque neurological disease genes." Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB125153 mRNA. Translation: BAD51941.1.
3D structure databases
ProteinModelPortal	Q60HH4.
ModBase	Search...
Protein family/group databases
MEROPS	C89.001.
Proteomic databases
PRIDE	Q60HH4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG050586.
Family and domain databases
InterPro	IPR016699. Acid_ceramidase-like. IPR003199. Chologlycine_hydro. [Graphical view]
Pfam	PF02275. CBAH. 1 hit. [Graphical view]
PIRSF	PIRSF017632. Acid_ceramidase-like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASAH1_MACFA

Accession

Primary (citable) accession number: Q60HH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	November 23, 2004
Last modified:	October 19, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program