Reviewed, UniProtKB/Swiss-Prot Q60HG4 (NB5R3_MACFA)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 3
      Short name=Cytochrome b5 reductase
      Short name=B5R
    EC=1.6.2.2
Alternative name(s):
    Diaphorase-1
Cleaved into the following 2 chains:
    1- Recommended name:
            NADH-cytochrome b5 reductase 3 membrane-bound form
    2- Recommended name:
            NADH-cytochrome b5 reductase 3 soluble form

Gene names

Name:	CYB5R3
Synonyms:	DIA1
ORF Names:	QccE-21146

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subunit structure	Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MOSC2 By similarity.
Subcellular location	Isoform 1: Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side By similarity. Mitochondrion outer membrane; Lipid-anchor; Cytoplasmic side By similarity. Isoform 2: Cytoplasm. Note: Produces the soluble form found in erythrocytes By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Cytoplasm Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Coding sequence diversity	Alternative promoter usage
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
PTM	Acetylation Lipoprotein Myristate Phosphoprotein
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]

Isoform 1 (identifier: Q60HG4-1)

Also known as: M;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q60HG4-2)

Also known as: S;

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Note: Produces the soluble form found in erythrocytes (By similarity).

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 301	300	NADH-cytochrome b5 reductase 3 membrane-bound form		PRO_0000019395
	Chain	27 – 301	275	NADH-cytochrome b5 reductase 3 soluble form		PRO_0000019397
Regions
	Domain	40 – 152	113	FAD-binding FR-type
	Nucleotide binding	132 – 147	16	FAD By similarity
	Nucleotide binding	171 – 206	36	FAD By similarity
Amino acid modifications
	Modified residue	42	1	N6-acetyllysine By similarity
	Modified residue	43	1	Phosphotyrosine By similarity
	Modified residue	120	1	N6-acetyllysine By similarity
	Modified residue	130	1	Phosphotyrosine By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity
Natural variations
	Alternative sequence	1 – 23	23	Missing in isoform 2.		VSP_013359

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (M) [UniParc]. Last modified January 23, 2007. Version 3. Checksum: FEAF8CF96B88DDEF	FASTA	301	34,142
	10 20 30 40 50 60 MGAQLSTLGH VVLSPVWFLY SLLMKLFRCS TPAITLESPD IKYSLRLIDR EIISHDTRRF 70 80 90 100 110 120 RFALPSPQHI LGLPVGQHIY LSARIDGNLV IRPYTPVSSD DDKGFVDLVI KVYFKDTHPK 130 140 150 160 170 180 FPAGGKMSQY LESMQIGDTI EFRGPNGLLV YQGKGKFAIR PDKKSNPVIK TVKSVGMIAG 190 200 210 220 230 240 GTGITPMLQV IRAIMKDPDD HTVCHLLFAN QTEKDILLRP ELEELRNEHS ARFKLWYTLD 250 260 270 280 290 300 RAPEAWDYSQ GFVNEEMIRD HLPPPEEEPL VLMCGPPPMI QYACLPNLDR VGHPKERCFA F
	Isoform 2 (S). Checksum: D1FBA9742F808D09	FASTA	278	31,628
	10 20 30 40 50 60 MKLFRCSTPA ITLESPDIKY SLRLIDREII SHDTRRFRFA LPSPQHILGL PVGQHIYLSA 70 80 90 100 110 120 RIDGNLVIRP YTPVSSDDDK GFVDLVIKVY FKDTHPKFPA GGKMSQYLES MQIGDTIEFR 130 140 150 160 170 180 GPNGLLVYQG KGKFAIRPDK KSNPVIKTVK SVGMIAGGTG ITPMLQVIRA IMKDPDDHTV 190 200 210 220 230 240 CHLLFANQTE KDILLRPELE ELRNEHSARF KLWYTLDRAP EAWDYSQGFV NEEMIRDHLP 250 260 270 PPEEEPLVLM CGPPPMIQYA CLPNLDRVGH PKERCFAF

References

[1]	"Isolation and characterization of cDNA for macaque neurological disease genes." Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB125163 mRNA. Translation: BAD51951.1.
3D structure databases
SMR	Q60HG4. Positions 30-300, 31-301.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q60HG4.
Enzyme and pathway databases
BRENDA	1.6.2.2. 3438.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NB5R3_MACFA

Accession

Primary (citable) accession number: Q60HG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 43 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)