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Q60HF9 (FUMH_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fumarate hydratase, mitochondrial

Short name=Fumarase
EC=4.2.1.2
Gene names
Name:FH
ORF Names:QorA-13820
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

(S)-malate = fumarate + H2O. HAMAP-Rule MF_00743

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP-Rule MF_00743

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00743

Subcellular location

Isoform Mitochondrial: Mitochondrion By similarity HAMAP-Rule MF_00743.

Isoform Cytoplasmic: Cytoplasm By similarity HAMAP-Rule MF_00743.

Post-translational modification

Isoform Cytoplasmic contains is acetylated at position 2 By similarity. HAMAP-Rule MF_00743

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.

Sequence similarities

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentCytoplasm
Mitochondrion
   Coding sequence diversityAlternative initiation
   DomainTransit peptide
   Molecular functionLyase
   PTMAcetylation
Gene Ontology (GO)
   Biological_processfumarate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

tricarboxylic acid cycle enzyme complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionfumarate hydratase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Mitochondrial (identifier: Q60HF9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Cytoplasmic (identifier: Q60HF9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.
Note: Initiator Met-1 is removed. Contains a N-acetylalanine at position 2 (By similarity).

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4444Mitochondrion By similarity
Chain45 – 510466Fumarate hydratase, mitochondrial HAMAP-Rule MF_00743
PRO_0000010321

Regions

Region176 – 1794B site By similarity
Region186 – 1883Substrate binding By similarity

Sites

Binding site1471Substrate By similarity

Amino acid modifications

Modified residue611N6-acetyllysine; alternate By similarity
Modified residue611N6-succinyllysine; alternate By similarity
Modified residue661N6-acetyllysine; alternate By similarity
Modified residue661N6-succinyllysine; alternate By similarity
Modified residue801N6-acetyllysine; alternate By similarity
Modified residue801N6-succinyllysine; alternate By similarity
Modified residue941N6-acetyllysine By similarity
Modified residue1151N6-acetyllysine; alternate By similarity
Modified residue1151N6-succinyllysine; alternate By similarity
Modified residue1221N6-acetyllysine; alternate By similarity
Modified residue1221N6-succinyllysine; alternate By similarity
Modified residue2131N6-acetyllysine By similarity
Modified residue2231N6-acetyllysine; alternate By similarity
Modified residue2231N6-succinyllysine; alternate By similarity
Modified residue2561N6-acetyllysine By similarity
Modified residue2921N6-acetyllysine; alternate By similarity
Modified residue2921N6-succinyllysine; alternate By similarity
Modified residue4671N6-succinyllysine By similarity
Modified residue4731N6-succinyllysine By similarity
Modified residue5021N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 4343Missing in isoform Cytoplasmic.
VSP_018966

Sequences

Sequence LengthMass (Da)Tools
Isoform Mitochondrial [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: F5FC970E977DEA09

FASTA51054,766
        10         20         30         40         50         60 
MYRALWLLAR SRRLVRPPAS ALASAPGLSG AAVPSFWPPN AARMASQNSF RIEYDTFGEL 

        70         80         90        100        110        120 
KVPNDKYYGA QTVRSTMNFK IGGVTERMPT PVIKAFGILK RAAAEVNQDY GLDPKIANAI 

       130        140        150        160        170        180 
MKAADEVAEG KLNDHFPLVV WQTGSGTQTN MNVNEVISNR AIEMLGGELG SKIPVHPNDH 

       190        200        210        220        230        240 
VNKSQSSNDT FPTAMHIAAA IEVHEVLLPG LQKLHDALDA KSKEFAQIIK IGRTHTQDAV 

       250        260        270        280        290        300 
PLTLGQEFSG YVQQVKYAVT RIKAAMPRIY ELAAGGTAVG TGLNTRIGFA EKVAAKVAAL 

       310        320        330        340        350        360 
TGLPFVTAPN KFEALAAHDA LVELSGAMNT TACSLMKIAN DIRFLGSGPR SGLGELILPE 

       370        380        390        400        410        420 
NEPGSSIMPG KVNPTQCEAM TMVAAQVMGN HVAVTVGGSN GHFELNVFKP MMIKNVLHSA 

       430        440        450        460        470        480 
RLLGDASVSF TENCVVGIQA NTERINKLMN ESLMLVTALN PHIGYDKAAK IAKTAHKNGS 

       490        500        510 
TLKETAIELG YLTAEQFDEW VKPKDMLGPK 

« Hide

Isoform Cytoplasmic [UniParc].

Checksum: 291AC7968C06254F
Show »

FASTA46750,181

References

[1]"Isolation and characterization of cDNA for macaque neurological disease genes."
Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Occipital cortex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB125168 mRNA. Translation: BAD51956.1.
UniGeneMfa.8229.

3D structure databases

ProteinModelPortalQ60HF9.
SMRQ60HF9. Positions 49-510.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ60HF9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG002183.

Enzyme and pathway databases

UniPathwayUPA00223; UER01007.

Family and domain databases

Gene3D1.10.275.10. 1 hit.
HAMAPMF_00743. FumaraseC.
InterProIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERPTHR11444. PTHR11444. 1 hit.
PfamPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSPR00149. FUMRATELYASE.
SUPFAMSSF48557. SSF48557. 1 hit.
TIGRFAMsTIGR00979. fumC_II. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFUMH_MACFA
AccessionPrimary (citable) accession number: Q60HF9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: November 23, 2004
Last modified: March 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways