Reviewed, UniProtKB/Swiss-Prot Q60HF6 (BGAL_MACFA)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase
    EC=3.2.1.23
Alternative name(s):
    Acid beta-galactosidase
      Short name=Lactase

Gene names

Name:	GLB1
ORF Names:	QccE-15801, QccE-17236

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	682 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Propeptide	24 – 28	5	By similarity		PRO_0000012188
	Chain	29 – 682	654	Beta-galactosidase		PRO_0000012189
Sites
	Active site	188	1	Proton donor Potential
	Active site	268	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	26	1	N-linked (GlcNAc...) Potential
	Glycosylation	247	1	N-linked (GlcNAc...) Potential
	Glycosylation	464	1	N-linked (GlcNAc...) Potential
	Glycosylation	498	1	N-linked (GlcNAc...) Potential
	Glycosylation	545	1	N-linked (GlcNAc...) Potential
	Glycosylation	555	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q60HF6-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: FDF97A8B8DC8B680	FASTA	682	76,826
	10 20 30 40 50 60 MPGFLVRILP LLLPLLLLGP TRGLRNATRR VFEIAYSQDR FLKDGQPFRY ISGSIHYSRV 70 80 90 100 110 120 PRFYWKDRLL KMKMAGLNTI QTYVPWNFHE PWPGQYQFSE DHDVEYFLRL AHELGLLVIL 130 140 150 160 170 180 RPGPYICAEW EMGGLPAWLL EKEAILLRSS DPDYLAAVDK WLGVLLPKMK PLLYQNGGPI 190 200 210 220 230 240 ITVQVENEYG SYFACDFDYL RFLQKRFHHH LGDDVVLFTT DGAHETFLQC GALQGLYTTV 250 260 270 280 290 300 DFGPGSNITD AFQIQRKCEP KGPLINSEFY TGWLDHWGQP HSTIKTEVVA SSLYDILARG 310 320 330 340 350 360 ASVNLYMFIG GTNFAYWNGA NSPYAAQPTS YDYDAPLSEA GDLTEKYFAL RNVIQKFEKV 370 380 390 400 410 420 PEGPIPPSTP KFAYGKVSLE KLKTVGAALD ILCPSGPIKS LYPLTFIQVK QYYGFVLYRT 430 440 450 460 470 480 TLPQDCSNST PLSSPFNGVH DRAYVAVDGI PQGVLERNRV ITLNITGKTG ATLDLLVENM 490 500 510 520 530 540 GRVNYGAYIN DFKGLVSNLT LDSNILTGWT IFPLDTEDAV RSHLGGWEHR DSGRHDEAWA 550 560 570 580 590 600 HSSSNYTLPA FYVGNFSIPS GIPDLPQDTF IQFPGWTKGQ VWINGFNLGR YWPARGPQLT 610 620 630 640 650 660 LFVPQHILMT SAPNTITVLE LERAPCSSDG PELCAVEFVD RPVIGSSQIY DHLSKPVEQR 670 680 LMAPPPKKTK IRGWSMYDDE SL

References

[1]	"Isolation and characterization of cDNA for macaque neurological disease genes." Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.
[2]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB125171 mRNA. Translation: BAD51959.1. AB169680 mRNA. Translation: BAE01761.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Phylogenomic databases
HOVERGEN	Q60HF6.
Enzyme and pathway databases
BRENDA	3.2.1.23. 3438.
Family and domain databases
InterPro	IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_sg_catalytic. IPR001944. Glycoside_Hdrlase_35. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_MACFA

Accession

Primary (citable) accession number: Q60HF6
Secondary accession number(s): Q4R564

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	November 23, 2004
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)