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Q60HF6 (BGAL_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

EC=3.2.1.23
Alternative name(s):
Acid beta-galactosidase
Short name=Lactase
Gene names
Name:GLB1
ORF Names:QccE-15801, QccE-17236
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length682 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans By similarity.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Lysosome By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
   Cellular componentLysosome
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Zymogen
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Propeptide24 – 285 By similarity
PRO_0000012188
Chain29 – 682654Beta-galactosidase
PRO_0000012189

Sites

Active site1881Proton donor Potential
Active site2681Nucleophile Potential

Amino acid modifications

Glycosylation261N-linked (GlcNAc...) Potential
Glycosylation2471N-linked (GlcNAc...) Potential
Glycosylation4641N-linked (GlcNAc...) Potential
Glycosylation4981N-linked (GlcNAc...) Potential
Glycosylation5451N-linked (GlcNAc...) Potential
Glycosylation5551N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q60HF6 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: FDF97A8B8DC8B680

FASTA68276,826
        10         20         30         40         50         60 
MPGFLVRILP LLLPLLLLGP TRGLRNATRR VFEIAYSQDR FLKDGQPFRY ISGSIHYSRV 

        70         80         90        100        110        120 
PRFYWKDRLL KMKMAGLNTI QTYVPWNFHE PWPGQYQFSE DHDVEYFLRL AHELGLLVIL 

       130        140        150        160        170        180 
RPGPYICAEW EMGGLPAWLL EKEAILLRSS DPDYLAAVDK WLGVLLPKMK PLLYQNGGPI 

       190        200        210        220        230        240 
ITVQVENEYG SYFACDFDYL RFLQKRFHHH LGDDVVLFTT DGAHETFLQC GALQGLYTTV 

       250        260        270        280        290        300 
DFGPGSNITD AFQIQRKCEP KGPLINSEFY TGWLDHWGQP HSTIKTEVVA SSLYDILARG 

       310        320        330        340        350        360 
ASVNLYMFIG GTNFAYWNGA NSPYAAQPTS YDYDAPLSEA GDLTEKYFAL RNVIQKFEKV 

       370        380        390        400        410        420 
PEGPIPPSTP KFAYGKVSLE KLKTVGAALD ILCPSGPIKS LYPLTFIQVK QYYGFVLYRT 

       430        440        450        460        470        480 
TLPQDCSNST PLSSPFNGVH DRAYVAVDGI PQGVLERNRV ITLNITGKTG ATLDLLVENM 

       490        500        510        520        530        540 
GRVNYGAYIN DFKGLVSNLT LDSNILTGWT IFPLDTEDAV RSHLGGWEHR DSGRHDEAWA 

       550        560        570        580        590        600 
HSSSNYTLPA FYVGNFSIPS GIPDLPQDTF IQFPGWTKGQ VWINGFNLGR YWPARGPQLT 

       610        620        630        640        650        660 
LFVPQHILMT SAPNTITVLE LERAPCSSDG PELCAVEFVD RPVIGSSQIY DHLSKPVEQR 

       670        680 
LMAPPPKKTK IRGWSMYDDE SL 

« Hide

References

[1]"Isolation and characterization of cDNA for macaque neurological disease genes."
Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain cortex.
[2]"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
International consortium for macaque cDNA sequencing and analysis
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain cortex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB125171 mRNA. Translation: BAD51959.1.
AB169680 mRNA. Translation: BAE01761.1.

3D structure databases

ProteinModelPortalQ60HF6.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PRIDEQ60HF6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG004841.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProIPR026283. B-gal_1-like.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF13364. BetaGal_dom4_5. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PIRSFPIRSF006336. B-gal. 1 hit.
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_MACFA
AccessionPrimary (citable) accession number: Q60HF6
Secondary accession number(s): Q4R564
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: November 23, 2004
Last modified: October 16, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries