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Q60HE5 (MTHR_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate reductase

EC=1.5.1.20
Gene names
Name:MTHFR
ORF Names:QtrA-17780
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length656 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine By similarity.

Catalytic activity

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactor

FAD By similarity.

Enzyme regulation

Allosterically regulated by S-adenosylmethionine By similarity.

Pathway

One-carbon metabolism; tetrahydrofolate interconversion.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
   LigandFAD
Flavoprotein
NADP
   Molecular functionOxidoreductase
   Technical termAllosteric enzyme
Gene Ontology (GO)
   Biological_processmethionine metabolic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionmethylenetetrahydrofolate reductase (NAD(P)H) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 656656Methylenetetrahydrofolate reductase
PRO_0000190246

Regions

Nucleotide binding63 – 686NAD By similarity
Nucleotide binding94 – 952NAD and FAD By similarity
Nucleotide binding157 – 1593FAD By similarity
Nucleotide binding174 – 1752FAD By similarity
Nucleotide binding201 – 2044FAD By similarity

Sites

Active site631Proton donor/acceptor By similarity
Binding site1271FAD By similarity
Binding site1591Substrate By similarity
Binding site1971FAD By similarity
Binding site2101FAD By similarity
Binding site2171FAD By similarity
Binding site2281Substrate By similarity
Binding site3211Substrate By similarity
Binding site3251Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q60HE5 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: AFF7FA239B9520FB

FASTA65674,656
        10         20         30         40         50         60 
MVNEARGNDS LNPCLEGSAS SSSESSKDSS RCSTPGLDPE RHERLRDKMR RRMESGDKWF 

        70         80         90        100        110        120 
SLEFFPPRTA EGAVNLISRF DRMAAGGPLF IDVTWHPAGD PGSDKETSSM MIASTAVNYC 

       130        140        150        160        170        180 
GLETILHMTC CCQRLEEITG HLHKAKQLGL KNIMALRGDP IGDQWEEEEG GFNYAVDLVK 

       190        200        210        220        230        240 
HIRSEFGDYF DLCVAGYPKG HPEAGSFEAD LKHLKEKVSA GADFIITQLF FEADTFFRFV 

       250        260        270        280        290        300 
KACTDMGITC PIVPGIFPIQ GYHSLRQLVK QSKLEVPQEI KDVIEPIKDN DAAIRNYGIE 

       310        320        330        340        350        360 
LAVSLCHELL ASGLVPGLHF YTLNREMATT EVLKRLGMWT EDPRRPLPWA LSAHPKRREE 

       370        380        390        400        410        420 
DVRPIFWASR PKSYIYRTQE WDEFPNGRWG NSSSPAFGEL KDYYLFYLKS KSPRELLLKM 

       430        440        450        460        470        480 
WGEELTSEES VFEVFVLYLS GEPNRNGHKV TCLPWNDEPL AAETSLLKEE LLRVNRQGIL 

       490        500        510        520        530        540 
TINSQPNING KPSSDPVVGW GPSGGYVFQK AYLEFFTSRE TAEALLQVLK KYELRVNYHL 

       550        560        570        580        590        600 
VNVKGENITN APELQPNAVT WGIFPGREII QPTVVDPISF MFWKDEAFAL WIEQWGKLYE 

       610        620        630        640        650 
EESPSRTIIQ YIHDNYFLVN LVDNDFPLDN CLWQVVEDAL ELLNRPTQNE REMEAP 

« Hide

References

[1]"Isolation and characterization of cDNA for macaque neurological disease genes."
Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Temporal cortex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB125182 mRNA. Translation: BAD51970.1.
RefSeqNP_001271578.1. NM_001284649.1.
UniGeneMfa.6344.

3D structure databases

ProteinModelPortalQ60HE5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID102117536.
KEGGmcf:102117536.

Organism-specific databases

CTD4524.

Phylogenomic databases

HOVERGENHBG006414.
KOK00297.

Enzyme and pathway databases

UniPathwayUPA00193.

Family and domain databases

Gene3D3.20.20.220. 1 hit.
InterProIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMSSF51730. SSF51730. 1 hit.
TIGRFAMsTIGR00677. fadh2_euk. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMTHR_MACFA
AccessionPrimary (citable) accession number: Q60HE5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: November 23, 2004
Last modified: June 11, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways