Reviewed, UniProtKB/Swiss-Prot Q60HE5 (MTHR_MACFA)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate reductase
    EC=1.5.1.20

Gene names

Name:	MTHFR
ORF Names:	QtrA-17780

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	656 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine By similarity.
Catalytic activity	5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Enzyme regulation	Allosterically regulated by S-adenosylmethionine By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	methionine metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 656	656	Methylenetetrahydrofolate reductase		PRO_0000190246

Sequences

	Sequence		Length	Mass (Da)
	Q60HE5-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: AFF7FA239B9520FB	FASTA	656	74,656
	10 20 30 40 50 60 MVNEARGNDS LNPCLEGSAS SSSESSKDSS RCSTPGLDPE RHERLRDKMR RRMESGDKWF 70 80 90 100 110 120 SLEFFPPRTA EGAVNLISRF DRMAAGGPLF IDVTWHPAGD PGSDKETSSM MIASTAVNYC 130 140 150 160 170 180 GLETILHMTC CCQRLEEITG HLHKAKQLGL KNIMALRGDP IGDQWEEEEG GFNYAVDLVK 190 200 210 220 230 240 HIRSEFGDYF DLCVAGYPKG HPEAGSFEAD LKHLKEKVSA GADFIITQLF FEADTFFRFV 250 260 270 280 290 300 KACTDMGITC PIVPGIFPIQ GYHSLRQLVK QSKLEVPQEI KDVIEPIKDN DAAIRNYGIE 310 320 330 340 350 360 LAVSLCHELL ASGLVPGLHF YTLNREMATT EVLKRLGMWT EDPRRPLPWA LSAHPKRREE 370 380 390 400 410 420 DVRPIFWASR PKSYIYRTQE WDEFPNGRWG NSSSPAFGEL KDYYLFYLKS KSPRELLLKM 430 440 450 460 470 480 WGEELTSEES VFEVFVLYLS GEPNRNGHKV TCLPWNDEPL AAETSLLKEE LLRVNRQGIL 490 500 510 520 530 540 TINSQPNING KPSSDPVVGW GPSGGYVFQK AYLEFFTSRE TAEALLQVLK KYELRVNYHL 550 560 570 580 590 600 VNVKGENITN APELQPNAVT WGIFPGREII QPTVVDPISF MFWKDEAFAL WIEQWGKLYE 610 620 630 640 650 EESPSRTIIQ YIHDNYFLVN LVDNDFPLDN CLWQVVEDAL ELLNRPTQNE REMEAP

References

[1]	"Isolation and characterization of cDNA for macaque neurological disease genes." Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Temporal cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB125182 mRNA. Translation: BAD51970.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q60HE5.
Family and domain databases
InterPro	IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00677. fadh2_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTHR_MACFA

Accession

Primary (citable) accession number: Q60HE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	November 23, 2004
Last modified:	November 25, 2008
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)