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Q60HE5

- MTHR_MACFA

UniProt

Q60HE5 - MTHR_MACFA

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Protein

Methylenetetrahydrofolate reductase

Gene

MTHFR

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine.By similarity

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD.By similarity

Enzyme regulationi

Allosterically regulated by S-adenosylmethionine.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei63 – 631Proton donor/acceptorBy similarity
Binding sitei127 – 1271FADBy similarity
Binding sitei159 – 1591SubstrateBy similarity
Binding sitei197 – 1971FADBy similarity
Binding sitei210 – 2101FADBy similarity
Binding sitei217 – 2171FADBy similarity
Binding sitei228 – 2281SubstrateBy similarity
Binding sitei321 – 3211SubstrateBy similarity
Binding sitei325 – 3251SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 686NADBy similarity
Nucleotide bindingi94 – 952NAD and FADBy similarity
Nucleotide bindingi157 – 1593FADBy similarity
Nucleotide bindingi174 – 1752FADBy similarity
Nucleotide bindingi201 – 2044FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine metabolic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:MTHFR
ORF Names:QtrA-17780
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 656656Methylenetetrahydrofolate reductasePRO_0000190246Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ60HE5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG006414.
KOiK00297.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q60HE5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNEARGNDS LNPCLEGSAS SSSESSKDSS RCSTPGLDPE RHERLRDKMR
60 70 80 90 100
RRMESGDKWF SLEFFPPRTA EGAVNLISRF DRMAAGGPLF IDVTWHPAGD
110 120 130 140 150
PGSDKETSSM MIASTAVNYC GLETILHMTC CCQRLEEITG HLHKAKQLGL
160 170 180 190 200
KNIMALRGDP IGDQWEEEEG GFNYAVDLVK HIRSEFGDYF DLCVAGYPKG
210 220 230 240 250
HPEAGSFEAD LKHLKEKVSA GADFIITQLF FEADTFFRFV KACTDMGITC
260 270 280 290 300
PIVPGIFPIQ GYHSLRQLVK QSKLEVPQEI KDVIEPIKDN DAAIRNYGIE
310 320 330 340 350
LAVSLCHELL ASGLVPGLHF YTLNREMATT EVLKRLGMWT EDPRRPLPWA
360 370 380 390 400
LSAHPKRREE DVRPIFWASR PKSYIYRTQE WDEFPNGRWG NSSSPAFGEL
410 420 430 440 450
KDYYLFYLKS KSPRELLLKM WGEELTSEES VFEVFVLYLS GEPNRNGHKV
460 470 480 490 500
TCLPWNDEPL AAETSLLKEE LLRVNRQGIL TINSQPNING KPSSDPVVGW
510 520 530 540 550
GPSGGYVFQK AYLEFFTSRE TAEALLQVLK KYELRVNYHL VNVKGENITN
560 570 580 590 600
APELQPNAVT WGIFPGREII QPTVVDPISF MFWKDEAFAL WIEQWGKLYE
610 620 630 640 650
EESPSRTIIQ YIHDNYFLVN LVDNDFPLDN CLWQVVEDAL ELLNRPTQNE

REMEAP
Length:656
Mass (Da):74,656
Last modified:November 23, 2004 - v1
Checksum:iAFF7FA239B9520FB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB125182 mRNA. Translation: BAD51970.1.
RefSeqiNP_001271578.1. NM_001284649.1.
UniGeneiMfa.6344.

Genome annotation databases

GeneIDi102117536.
KEGGimcf:102117536.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB125182 mRNA. Translation: BAD51970.1 .
RefSeqi NP_001271578.1. NM_001284649.1.
UniGenei Mfa.6344.

3D structure databases

ProteinModelPortali Q60HE5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 102117536.
KEGGi mcf:102117536.

Organism-specific databases

CTDi 4524.

Phylogenomic databases

HOVERGENi HBG006414.
KOi K00297.

Enzyme and pathway databases

UniPathwayi UPA00193 .

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Isolation and characterization of cDNA for macaque neurological disease genes."
    Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Temporal cortex.

Entry informationi

Entry nameiMTHR_MACFA
AccessioniPrimary (citable) accession number: Q60HE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: November 23, 2004
Last modified: October 1, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3