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Q60HE3

- NDUS8_MACFA

UniProt

Q60HE3 - NDUS8_MACFA

Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

NDUFS8

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi111 – 1111Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi114 – 1141Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi117 – 1171Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi121 – 1211Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi150 – 1501Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi153 – 1531Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi156 – 1561Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi160 – 1601Iron-sulfur 1 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    GO - Biological processi

    1. mitochondrial respiratory chain complex I assembly Source: UniProtKB
    2. response to oxidative stress Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-23kD
    Short name:
    CI-23kD
    NADH-ubiquinone oxidoreductase 23 kDa subunit
    Gene namesi
    Name:NDUFS8
    ORF Names:QorA-12386
    OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
    Taxonomic identifieri9541 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: UniProtKB
    2. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3434MitochondrionBy similarityAdd
    BLAST
    Chaini35 – 210176NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialPRO_0000020013Add
    BLAST

    Proteomic databases

    PRIDEiQ60HE3.

    Interactioni

    Subunit structurei

    Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ60HE3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini102 – 131304Fe-4S ferredoxin-type 1Add
    BLAST
    Domaini141 – 170304Fe-4S ferredoxin-type 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 23 kDa subunit family.Curated
    Contains 2 4Fe-4S ferredoxin-type domains.Curated

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    HOVERGENiHBG006547.

    Family and domain databases

    HAMAPiMF_01351. NDH1_NuoI.
    InterProiIPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view]
    PfamiPF12838. Fer4_7. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01971. NuoI. 1 hit.
    PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q60HE3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRCLTMPTLL RALAQAAHTG PPGGRTLHSS AVAATYKYVN MQESKTDMKS    50
    VTDRAARTLL WTELFRGLGM TLSYLFREPA TINYPFEKGP LSPRFRGEHA 100
    LRRYPSGEER CIACKLCEAV CPAQAITIEA EPRADGSRRT TRYDIDMTKC 150
    IYCGFCQEAC PVDAIVEGPN FEFSTETHEE LLYNKEKLLN NGDKWEAEIA 200
    ANIQADYLYR 210
    Length:210
    Mass (Da):23,689
    Last modified:November 23, 2004 - v1
    Checksum:i29F1E397F5B75045
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB125184 mRNA. Translation: BAD51972.1.
    UniGeneiMfa.8764.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB125184 mRNA. Translation: BAD51972.1 .
    UniGenei Mfa.8764.

    3D structure databases

    ProteinModelPortali Q60HE3.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q60HE3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG006547.

    Family and domain databases

    HAMAPi MF_01351. NDH1_NuoI.
    InterProi IPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view ]
    Pfami PF12838. Fer4_7. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01971. NuoI. 1 hit.
    PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of cDNA for macaque neurological disease genes."
      Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Occipital cortex.

    Entry informationi

    Entry nameiNDUS8_MACFA
    AccessioniPrimary (citable) accession number: Q60HE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3