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Q60HE3 (NDUS8_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
Complex I-23kD
Short name=CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit
Gene names
Name:NDUFS8
ORF Names:QorA-12386
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. HAMAP-Rule MF_01351

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out). HAMAP-Rule MF_01351

NADH + acceptor = NAD+ + reduced acceptor. HAMAP-Rule MF_01351

Cofactor

Binds 2 4Fe-4S clusters per subunit By similarity. HAMAP-Rule MF_01351

Subunit structure

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.

Subcellular location

Mitochondrion By similarity HAMAP-Rule MF_01351.

Sequence similarities

Belongs to the complex I 23 kDa subunit family.

Contains 2 4Fe-4S ferredoxin-type domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3434Mitochondrion By similarity
Chain35 – 210176NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial HAMAP-Rule MF_01351
PRO_0000020013

Regions

Domain102 – 131304Fe-4S ferredoxin-type 1
Domain141 – 170304Fe-4S ferredoxin-type 2

Sites

Metal binding1111Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1141Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1171Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1211Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1501Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1531Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1561Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1601Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q60HE3 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 29F1E397F5B75045

FASTA21023,689
        10         20         30         40         50         60 
MRCLTMPTLL RALAQAAHTG PPGGRTLHSS AVAATYKYVN MQESKTDMKS VTDRAARTLL 

        70         80         90        100        110        120 
WTELFRGLGM TLSYLFREPA TINYPFEKGP LSPRFRGEHA LRRYPSGEER CIACKLCEAV 

       130        140        150        160        170        180 
CPAQAITIEA EPRADGSRRT TRYDIDMTKC IYCGFCQEAC PVDAIVEGPN FEFSTETHEE 

       190        200        210 
LLYNKEKLLN NGDKWEAEIA ANIQADYLYR 

« Hide

References

[1]"Isolation and characterization of cDNA for macaque neurological disease genes."
Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Occipital cortex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB125184 mRNA. Translation: BAD51972.1.
UniGeneMfa.8764.

3D structure databases

ProteinModelPortalQ60HE3.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ60HE3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG006547.

Family and domain databases

HAMAPMF_01351. NDH1_NuoI.
InterProIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsTIGR01971. NuoI. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNDUS8_MACFA
AccessionPrimary (citable) accession number: Q60HE3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families