Reviewed, UniProtKB/Swiss-Prot Q60HD7 (SERA_MACFA)

Names and origin

Protein names

Recommended name:
    D-3-phosphoglycerate dehydrogenase
      Short name=3-PGDH
    EC=1.1.1.95

Gene names

Name:	PHGDH
ORF Names:	QccE-10982, QccE-21483

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH. 2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glyceric acid: step 1/3.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro phosphoglycerate dehydrogenase activity Inferred from electronic annotation. Source: EC transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 533	532	D-3-phosphoglycerate dehydrogenase		PRO_0000076013
Sites
	Active site	236	1	By similarity
	Active site	265	1	By similarity
	Active site	283	1	Proton donor By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	371	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	95	1	V → G in BAE01893. Ref.2
	Sequence conflict	178	1	I → V in BAE01893. Ref.2
	Sequence conflict	442	1	T → K in BAD51978. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q60HD7-1 [UniParc]. Last modified February 5, 2008. Version 4. Checksum: 92875C1F602A0956	FASTA	533	56,518
	10 20 30 40 50 60 MAFANLRKVL ISDSLDPCCR KILQDGGLQV VEKQNLSKEE LIAELQDCEG LIVRSATKVT 70 80 90 100 110 120 ADVINAAEKL QVVGRAGTGV DNVDLEAATR KGVLVMNTPN GNSLSAAELT CGMIMCLARQ 130 140 150 160 170 180 IPQATASMKD GKWERKKFMG TELNGKTLGI LGLGRIGREV ATRMQSFGMK TIGYDPIISP 190 200 210 220 230 240 EVSASFGVQQ LPLEEIWPLC DFITVHTPLL PSTTGLLNDN TFAQCKKGVR VVNCARGGIV 250 260 270 280 290 300 DEGALLRALQ SGQCAGAALD VFTEEPPRDR ALVDHENVIS CPHLGASTKE AQSRCGEEIA 310 320 330 340 350 360 VQFVDMVKGK SLAGVVNAQA LTSAFSPHTK PWIGLAEALG TLMRAWAGSP KGTIQVITQG 370 380 390 400 410 420 TSLKNAGNCL SPAVIVGLLK EASKQADVNL VNAKLLVKEA GLDVTTSHSP AAPGEQGFGE 430 440 450 460 470 480 CLLAVALAGA PYQAVGLVQG TTPVLQGLNG AVFRPEVPLR RGLPLLLFRT QTSDPAMLPT 490 500 510 520 530 MIGLLAEAGV RLLSYQTSLV SDGETWHVMG ISSLLPSLEA WKPHVTEAFQ FHF

References

[1]	"Isolation and characterization of cDNA for macaque neurological disease genes." Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.
[2]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB125190 mRNA. Translation: BAD51978.1. AB169812 mRNA. Translation: BAE01893.1.
3D structure databases
SMR	Q60HD7. Positions 5-306, 6-307.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q60HD7.
Enzyme and pathway databases
BRENDA	1.1.1.95. 3438.
Family and domain databases
InterPro	IPR001588. Casein. IPR006236. D-3-Phosphoglycerate_DH. IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR015508. D3PG_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10996:SF20. D3PG_Deh. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01327. PGDH. 1 hit.
PROSITE	PS00306. CASEIN_ALPHA_BETA. 1 hit. PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERA_MACFA

Accession

Primary (citable) accession number: Q60HD7
Secondary accession number(s): Q4R4T2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	February 5, 2008
Last modified:	June 16, 2009
This is version 35 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)