Q60HD5 (PPOX_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protoporphyrinogen oxidase
Short name=PPO
EC=1.3.3.4

Gene names

Name:	PPOX
ORF Names:	QccE-13968

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Cercopithecoidea › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX By similarity.
Catalytic activity	Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.
Cofactor	Binds 1 FAD per dimer By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side By similarity. Note: Bound to the mitochondrial inner membrane with its active site facing the cytosolic side By similarity.
Sequence similarities	Belongs to the protoporphyrinogen oxidase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	heme biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	intrinsic to mitochondrial inner membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxygen-dependent protoporphyrinogen oxidase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Protoporphyrinogen oxidase		PRO_0000135271
Regions
	Nucleotide binding	9 – 14	6	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q60HD5 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 5665D8871470AEA5	FASTA	477	50,613
	10 20 30 40 50 60 MGRTVVVLGG GISGLAASYH LSRAPCPPKV VLVEGSERLG GWIRSVRGPN GAIFELGPRG 70 80 90 100 110 120 IRPAGALGAR TLLLVSELGL DSEVLPVRGD HPAAQNRFLY VGGALHALPT GLRGLLRPSP 130 140 150 160 170 180 PFSKPLFWAG LRELTKPRGK EPDETVHSFA QRRLGPEVAS LAMDSLCRGV FAGNSRELSI 190 200 210 220 230 240 RSCFPSLFQA EQTHRSVLLG LLLGAGRTPQ PDSALIRQAL AERWSQWSLR GGLEMLPQAL 250 260 270 280 290 300 ETHLTSRGVS VLRGQPVCGL SLQAEGRWKV SLRDSSLEAD HVISAIPASV LSELLPAEAA 310 320 330 340 350 360 PLARALSAIT AVSVAVVNLQ YQGAHLPVQG FGHLVPSSED PGVLGIVYDS VAFPEQDGSP 370 380 390 400 410 420 PGLRVTVMLG GSWLQTLEAS GCVLSQELFQ QRAQEAAAAQ LGLKELPSHC LVHLHKNCIP 430 440 450 460 470 QYTLGHWQKL ESARQFLAAH RLPLTLAGAS YEGVAVNDCI ESGRQAAVSV LGTEPNG

References

[1]	"Isolation and characterization of cDNA for macaque neurological disease genes." Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB125192 mRNA. Translation: BAD51980.1.
3D structure databases
ProteinModelPortal	Q60HD5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG001709.
Enzyme and pathway databases
UniPathway	UPA00251; UER00324.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002937. Amino_oxidase. IPR016040. NAD(P)-bd_dom. IPR004572. Protoporphyrinogen_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00562. proto_IX_ox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPOX_MACFA

Accession

Primary (citable) accession number: Q60HD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	November 23, 2004
Last modified:	March 6, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program