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Q60HC8

- UCHL1_MACFA

UniProt

Q60HC8 - UCHL1_MACFA

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Protein
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Gene
UCHL1, QccE-15749
Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei1 – 11Susceptible to oxidation By similarity
Sitei6 – 61Susceptible to oxidation By similarity
Sitei12 – 121Susceptible to oxidation By similarity
Active sitei90 – 901Nucleophile By similarity
Sitei124 – 1241Susceptible to oxidation By similarity
Active sitei161 – 1611Proton donor By similarity
Sitei176 – 1761Important for enzyme activity By similarity
Sitei179 – 1791Susceptible to oxidation By similarity
Sitei220 – 2201Susceptible to oxidation By similarity

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. ubiquitin-specific protease activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BRENDAi3.4.19.12. 3121.

Protein family/group databases

MEROPSiC12.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
Short name:
UCH-L1
Alternative name(s):
Ubiquitin thioesterase L1
Gene namesi
Name:UCHL1
ORF Names:QccE-15749
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Cytoplasm By similarity. Endoplasmic reticulum membrane; Lipid-anchor By similarity. Nucleus By similarity
Note: Found both cytoplasmic and nuclear in cytotrophoblasts and decidual cells.1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Ubiquitin carboxyl-terminal hydrolase isozyme L1
PRO_0000211056Add
BLAST
Propeptidei221 – 2233Removed in mature form By similarity
PRO_0000414312

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi220 – 2201S-farnesyl cysteine By similarity

Post-translational modificationi

O-glycosylated By similarity.

Keywords - PTMi

Glycoprotein, Lipoprotein, Oxidation, Prenylation

Proteomic databases

PRIDEiQ60HC8.

Expressioni

Tissue specificityi

Expressed in the placenta at all stages of pregnancy. Expression increases as pregnancy progresses.1 Publication

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with COPS5 and SNCA By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ60HC8.
SMRiQ60HC8. Positions 1-223.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni5 – 106Interaction with ubiquitin By similarity
Regioni151 – 1566Interaction with ubiquitin By similarity
Regioni211 – 2166Interaction with ubiquitin By similarity

Sequence similaritiesi

Belongs to the peptidase C12 family.

Phylogenomic databases

HOVERGENiHBG075483.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiPS00140. UCH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q60HC8-1 [UniParc]FASTAAdd to Basket

« Hide

MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEDSLG SVPAPACALL    50
LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA 100
NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG 150
QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE GTLLQDAAKV 200
CREFTEREQG EVRFSAVALC KAA 223
Length:223
Mass (Da):24,752
Last modified:November 23, 2004 - v1
Checksum:iCB15EDCD56B5D03B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB125199 mRNA. Translation: BAD51987.1.
UniGeneiMfa.1339.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB125199 mRNA. Translation: BAD51987.1 .
UniGenei Mfa.1339.

3D structure databases

ProteinModelPortali Q60HC8.
SMRi Q60HC8. Positions 1-223.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C12.001.

Proteomic databases

PRIDEi Q60HC8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG075483.

Enzyme and pathway databases

BRENDAi 3.4.19.12. 3121.

Family and domain databases

Gene3Di 3.40.532.10. 1 hit.
InterProi IPR001578. Peptidase_C12_UCH.
[Graphical view ]
PANTHERi PTHR10589. PTHR10589. 1 hit.
Pfami PF01088. Peptidase_C12. 1 hit.
[Graphical view ]
PRINTSi PR00707. UBCTHYDRLASE.
PROSITEi PS00140. UCH_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of cDNA for macaque neurological disease genes."
    Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain cortex.
  2. "Localization of ubiquitin carboxyl-terminal hydrolase-L1 in cynomolgus monkey placentas."
    Sekiguchi S., Takatori A., Negishi T., Kwon J., Kokubo T., Ishii Y., Kyuwa S., Yoshikawa Y.
    Placenta 26:99-103(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiUCHL1_MACFA
AccessioniPrimary (citable) accession number: Q60HC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8 By similarity.

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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