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Q60HC8 (UCHL1_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 6, 2013. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Short name=UCH-L1
EC=3.4.19.12
EC=6.-.-.-
Alternative name(s):
Ubiquitin thioesterase L1
Gene names
Name:UCHL1
ORF Names:QccE-15749
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Monomer. Homodimer. Interacts with COPS5 and SNCA By similarity.

Subcellular location

Cytoplasm By similarity. Endoplasmic reticulum membrane; Lipid-anchor By similarity. Nucleus By similarity. Note: Found both cytoplasmic and nuclear in cytotrophoblasts and decidual cells. Ref.2

Tissue specificity

Expressed in the placenta at all stages of pregnancy. Expression increases as pregnancy progresses. Ref.2

Post-translational modification

O-glycosylated By similarity.

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8 By similarity.

Sequence similarities

Belongs to the peptidase C12 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220Ubiquitin carboxyl-terminal hydrolase isozyme L1
PRO_0000211056
Propeptide221 – 2233Removed in mature form By similarity
PRO_0000414312

Regions

Region5 – 106Interaction with ubiquitin By similarity
Region151 – 1566Interaction with ubiquitin By similarity
Region211 – 2166Interaction with ubiquitin By similarity

Sites

Active site901Nucleophile By similarity
Active site1611Proton donor By similarity
Site11Susceptible to oxidation By similarity
Site61Susceptible to oxidation By similarity
Site121Susceptible to oxidation By similarity
Site1241Susceptible to oxidation By similarity
Site1761Important for enzyme activity By similarity
Site1791Susceptible to oxidation By similarity
Site2201Susceptible to oxidation By similarity

Amino acid modifications

Lipidation2201S-farnesyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q60HC8 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: CB15EDCD56B5D03B

FASTA22324,752
        10         20         30         40         50         60 
MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEDSLG SVPAPACALL LLFPLTAQHE 

        70         80         90        100        110        120 
NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE 

       130        140        150        160        170        180 
TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP 

       190        200        210        220 
FPVNHGASSE GTLLQDAAKV CREFTEREQG EVRFSAVALC KAA

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of cDNA for macaque neurological disease genes."
Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain cortex.
[2]"Localization of ubiquitin carboxyl-terminal hydrolase-L1 in cynomolgus monkey placentas."
Sekiguchi S., Takatori A., Negishi T., Kwon J., Kokubo T., Ishii Y., Kyuwa S., Yoshikawa Y.
Placenta 26:99-103(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB125199 mRNA. Translation: BAD51987.1.

3D structure databases

ProteinModelPortalQ60HC8.
SMRQ60HC8. Positions 1-223.
ModBaseSearch...

Protein family/group databases

MEROPSC12.001.

Proteomic databases

PRIDEQ60HC8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG075483.

Enzyme and pathway databases

BRENDA3.4.19.12. 3121.

Family and domain databases

Gene3D3.40.532.10. 1 hit.
InterProIPR001578. Peptidase_C12.
[Graphical view]
PANTHERPTHR10589. PTHR10589. 1 hit.
PfamPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSPR00707. UBCTHYDRLASE.
PROSITEPS00140. UCH_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUCHL1_MACFA
AccessionPrimary (citable) accession number: Q60HC8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: November 23, 2004
Last modified: March 6, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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