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Protein

Phosphatidylinositol-glycan biosynthesis class X protein

Gene

Pigx

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM.1 Publication

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol-glycan biosynthesis class X protein
Short name:
PIG-X
Gene namesi
Name:Pigx
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1307289. Pigx.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei225 – 245HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000024629723 – 252Phosphatidylinositol-glycan biosynthesis class X proteinAdd BLAST230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi97N-linked (GlcNAc...)Sequence analysis1
Glycosylationi209N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ60GF7.
PRIDEiQ60GF7.

Interactioni

Subunit structurei

Interacts with PIGM.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000042305.

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGX family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGQT. Eukaryota.
ENOG410Y4AX. LUCA.
HOGENOMiHOG000015227.
HOVERGENiHBG060417.
InParanoidiQ60GF7.
KOiK07541.
PhylomeDBiQ60GF7.

Family and domain databases

InterProiIPR013233. PIG-X/PBN1.
[Graphical view]
PfamiPF08320. PIG-X. 1 hit.
[Graphical view]
SMARTiSM00780. PIG-X. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q60GF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASALAWLL LWAAGLVGRL AADISDARFS DGVRATCSEI ILRQEFLKDG
60 70 80 90 100
FHRDLLIKVK FGESIEDLQT CRLLIKHYIP TGLFVDPYEL ASLRERNITE
110 120 130 140 150
AVMVSESFNL EAPNYLSTES AVLIYARQDA QCIDCFQAFL PVHYRYHRPH
160 170 180 190 200
KKDGDTLIVV NNPDLLMHCD QEFPILKCWA QSEVAAPCSL KSEEICQWKN
210 220 230 240 250
MQYKSILKNL TVQVPVGLTI HTSLVCSVTL LITVLCSTLI LLAVFKYGHF

SL
Length:252
Mass (Da):28,423
Last modified:July 25, 2006 - v2
Checksum:i28AE1E78438A3A85
GO

Sequence cautioni

The sequence BAD61008 differs from that shown. Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB177393 mRNA. Translation: BAD61008.1. Sequence problems.
RefSeqiNP_001094121.1. NM_001100651.1.
UniGeneiRn.34037.

Genome annotation databases

GeneIDi288041.
KEGGirno:288041.
UCSCiRGD:1307289. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB177393 mRNA. Translation: BAD61008.1. Sequence problems.
RefSeqiNP_001094121.1. NM_001100651.1.
UniGeneiRn.34037.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000042305.

Proteomic databases

PaxDbiQ60GF7.
PRIDEiQ60GF7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi288041.
KEGGirno:288041.
UCSCiRGD:1307289. rat.

Organism-specific databases

CTDi54965.
RGDi1307289. Pigx.

Phylogenomic databases

eggNOGiENOG410IGQT. Eukaryota.
ENOG410Y4AX. LUCA.
HOGENOMiHOG000015227.
HOVERGENiHBG060417.
InParanoidiQ60GF7.
KOiK07541.
PhylomeDBiQ60GF7.

Enzyme and pathway databases

UniPathwayiUPA00196.

Miscellaneous databases

PROiQ60GF7.

Family and domain databases

InterProiIPR013233. PIG-X/PBN1.
[Graphical view]
PfamiPF08320. PIG-X. 1 hit.
[Graphical view]
SMARTiSM00780. PIG-X. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIGX_RAT
AccessioniPrimary (citable) accession number: Q60GF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: June 8, 2016
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

PubMed:15635094 reported that the initiator methionine is coded by an unusual start codon, CTG.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.