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Q60CV4 (TRUA_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase A

EC=5.4.99.12
Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I
Gene names
Name:truA
Ordered Locus Names:BRA1033, BS1330_II1025
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171

Catalytic activity

tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00171

Sequence similarities

Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251tRNA pseudouridine synthase A HAMAP-Rule MF_00171
PRO_0000057347

Sites

Active site521Nucleophile By similarity
Binding site1131Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q60CV4 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: E2A62312443C2BCD

FASTA25128,172
        10         20         30         40         50         60 
MPRYKLTVEY DGTPYVGWQR QENGHAVQGA IEQAFKKFCG EDLTLSAAGR TDAGVHATAQ 

        70         80         90        100        110        120 
VAHVDLAKDW GAGKVRDAVN AHLVMADERI SILNVEKTTD TFDARFSARA RHYLYRIHNR 

       130        140        150        160        170        180 
RAPLAVDYQR AWWVQKQLDA DAMHEAAQRL LGEHDFTTFR ATQCQAKSPV KTLDRLDVTR 

       190        200        210        220        230        240 
NGDMVEMRVS ARSFLHNQVR SFAGSLMEVG VGRWTADDLQ AALEARDRKA CGQVAPPYGL 

       250 
YLVGVDYAFP F 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014292 Genomic DNA. Translation: AAV28867.1.
CP002998 Genomic DNA. Translation: AEM20477.1.
RefSeqYP_002519393.1. NC_004311.2.
YP_005614703.1. NC_017250.1.

3D structure databases

ProteinModelPortalQ60CV4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BRA1033.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV28867; AAV28867; BRA1033.
AEM20477; AEM20477; BS1330_II1025.
GeneID1165478.
12139258.
KEGGbms:BRA1033.
bsi:BS1330_II1025.
PATRIC17794901. VBIBruSui107850_3258.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0101.
HOGENOMHOG000248672.
KOK06173.
OrthoDBEOG6Z9B4R.

Family and domain databases

Gene3D3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPMF_00171. TruA.
InterProIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERPTHR11142. PTHR11142. 1 hit.
PfamPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMSSF55120. SSF55120. 1 hit.
TIGRFAMsTIGR00071. hisT_truA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRUA_BRUSU
AccessionPrimary (citable) accession number: Q60CV4
Secondary accession number(s): G0KE39
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella suis

Brucella suis (strain 1330): entries and gene names