Q60CV4 (TRUA_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A
EC=5.4.99.12

Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I

Gene names

Name:	truA
Ordered Locus Names:	BRA1033, BS1330_II1025

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171
Catalytic activity	tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	tRNA pseudouridine synthase A HAMAP-Rule MF_00171		PRO_0000057347
Sites
	Active site	52	1	Nucleophile By similarity
	Binding site	113	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60CV4 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: E2A62312443C2BCD	FASTA	251	28,172
	10 20 30 40 50 60 MPRYKLTVEY DGTPYVGWQR QENGHAVQGA IEQAFKKFCG EDLTLSAAGR TDAGVHATAQ 70 80 90 100 110 120 VAHVDLAKDW GAGKVRDAVN AHLVMADERI SILNVEKTTD TFDARFSARA RHYLYRIHNR 130 140 150 160 170 180 RAPLAVDYQR AWWVQKQLDA DAMHEAAQRL LGEHDFTTFR ATQCQAKSPV KTLDRLDVTR 190 200 210 220 230 240 NGDMVEMRVS ARSFLHNQVR SFAGSLMEVG VGRWTADDLQ AALEARDRKA CGQVAPPYGL 250 YLVGVDYAFP F

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014292 Genomic DNA. Translation: AAV28867.1. CP002998 Genomic DNA. Translation: AEM20477.1.
RefSeq	YP_002519393.1. NC_004311.2. YP_005614703.1. NC_017250.1.
3D structure databases
ProteinModelPortal	Q60CV4.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BRA1033.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV28867; AAV28867; BRA1033. AEM20477; AEM20477; BS1330_II1025.
GeneID	1165478. 12139258.
KEGG	bms:BRA1033. bsi:BS1330_II1025.
PATRIC	17794901. VBIBruSui107850_3258.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0101.
HOGENOM	HOG000248672.
KO	K06173.
ProtClustDB	PRK00021.
Family and domain databases
Gene3D	3.30.70.580. 1 hit. 3.30.70.660. 1 hit.
HAMAP	MF_00171. TruA.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
PANTHER	PTHR11142. PTHR11142. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_BRUSU

Accession

Primary (citable) accession number: Q60CV4
Secondary accession number(s): G0KE39

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program