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Q60CK8 (GATA_METCA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A

Short name=Glu-ADT subunit A
EC=6.3.5.7
Gene names
Name:gatA
Ordered Locus Names:MCA0098
OrganismMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]
Taxonomic identifier243233 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00120

Catalytic activity

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00120

Subunit structure

Heterotrimer of A, B and C subunits By similarity. HAMAP-Rule MF_00120

Sequence similarities

Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 485485Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP-Rule MF_00120
PRO_0000241117

Sites

Active site761Charge relay system By similarity
Active site1511Charge relay system By similarity
Active site1751Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q60CK8 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: CAD9800148C2125D

FASTA48552,080
        10         20         30         40         50         60 
MHRKTLAELA AGLERREFSS VELTQAHLAR IERLDPALNS FITTTPEIAL AQARAADERL 

        70         80         90        100        110        120 
AKGEAGPLTG IPIAQKDIFC TKGVRTSCGS RMLDSFVSPY DACVVERFNA AGAVMLGKLN 

       130        140        150        160        170        180 
MDEFAMGSSN ETSYYGPVKN PWNTATVPGG SSGGSAAAVA ARLVPGATGT DTGGSIRQPA 

       190        200        210        220        230        240 
AFCGITGLKP TYGRVSRWGM IAFASSLDQA GPMARTAEDC AIMLQIMAGF DERDSTCVDR 

       250        260        270        280        290        300 
PVPDYRAALG NDLDGLRIGL PKEFFGEGLD PAIAGLIHAA VDEYRRLGAI VREISLPNMH 

       310        320        330        340        350        360 
LSVPAYYVVA PAECSSNLAR FDGVRFGHRC ENPTDLADLY TRSRGEGFGA EVKRRILIGT 

       370        380        390        400        410        420 
YALSAGYYDA YYLKAQKIRR LISEDFRRAF EEVDVIMGPT APSVAFEFGA KSADPIAMYL 

       430        440        450        460        470        480 
SDIYTIAVNL AGLPGMSIPV GFSNGLPVGM QIIGGYFSED RLLNVAHRYQ QATDWHTRTP 


AGIAD 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017282 Genomic DNA. Translation: AAU90722.1.
RefSeqYP_112637.1. NC_002977.6.

3D structure databases

ProteinModelPortalQ60CK8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243233.MCA0098.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU90722; AAU90722; MCA0098.
GeneID3102578.
KEGGmca:MCA0098.
PATRIC22603921. VBIMetCap22254_0104.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0154.
HOGENOMHOG000116699.
KOK02433.
OMAGKPFAEE.
OrthoDBEOG61P6R9.

Family and domain databases

Gene3D3.90.1300.10. 1 hit.
HAMAPMF_00120. GatA.
InterProIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERPTHR11895. PTHR11895. 1 hit.
PfamPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMSSF75304. SSF75304. 1 hit.
TIGRFAMsTIGR00132. gatA. 1 hit.
PROSITEPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGATA_METCA
AccessionPrimary (citable) accession number: Q60CK8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families