Q60BX0 (GLO2_METCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	MCA0343

Organism

Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]

Taxonomic identifier

243233 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: InterPro hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000309661
Sites
	Metal binding	55	1	Zinc 1 By similarity
	Metal binding	57	1	Zinc 1 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	60	1	Zinc 2 By similarity
	Metal binding	113	1	Zinc 1 By similarity
	Metal binding	132	1	Zinc 1 By similarity
	Metal binding	132	1	Zinc 2 By similarity
	Metal binding	170	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60BX0 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: ACA09A8A5D8812E2	FASTA	256	27,829
	10 20 30 40 50 60 MLEILQIPVL EDNYVYLLHE PGSGATAAVD PAVAGPVLEA LDARGWRLGH VLNTHHHGDH 70 80 90 100 110 120 VGGNLELKAA TGCTVVGAAG DRHRIPGIDV ALKDGEEFRL GSASARMLDV PGHTSGHVAF 130 140 150 160 170 180 WFEDDAALFC GDTLFALGCG RLFEGSAEQM WRSLERLRAL PAETKVFCAH EYTQANARFA 190 200 210 220 230 240 VTIEPGNAAL RERVERVEAL RREGAATVPS ILSEELATNP FLRPESPEIR ARLGLPGVPE 250 VEVFAEIRRR KDVFRG

References

[1]

"Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath)." Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S., Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E., Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J. , Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L., Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H., Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R., Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A. PLoS Biol. 2:1616-1628(2004) [PubMed: 15383840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33009 / NCIMB 11132 / Bath.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017282 Genomic DNA. Translation: AAU90561.1.
RefSeq	YP_112875.1. NC_002977.6.
3D structure databases
HSSP	HSSP built from PDB template 1XM8 based on UniProtKB Q9SID3.
ProteinModelPortal	Q60BX0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3102760.
GenomeReviews	Gene locus MCA0343 in contig AE017282_GR.
KEGG	mca:MCA0343.
NMPDR	fig|243233.4.peg.713.
PATRIC	22604427. VBIMetCap22254_0355.
TIGR	MCA0343.
Phylogenomic databases
HOGENOM	HBG753931.
OMA	ITHHHRD.
ProtClustDB	CLSK931492.
Enzyme and pathway databases
BioCyc	MCAP243233:MCA_0343-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR001018. Beta-lactamase_class-B_CS. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_METCA

Accession

Primary (citable) accession number: Q60BX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program