Q60BW3 (GCST_METCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	MCA0350

Organism

Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]

Taxonomic identifier

243233 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Aminomethyltransferase HAMAP MF_00259		PRO_0000122570

Sequences

	Sequence		Length	Mass (Da)
	Q60BW3 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: FC2B7DD8D1B0FB8C	FASTA	360	39,078
	10 20 30 40 50 60 MGRCTALHEE HLALGARMTE FSGWELPLHY GSQIAEHHAV RRAAGMFDVS HLGVVDVEGL 70 80 90 100 110 120 QAAPFLRRVL ANDVARLAEP GRMLYGCMLN QDGGIVDDLV VGFIDDRRFR LILNAGTREK 130 140 150 160 170 180 DLSWLHRQAA PFSVTVTPRD DLAMIALQGP DSPRIADAVV AAGSSGLKPF TATQRGDRFI 190 200 210 220 230 240 ARTGYTGEDG FEIILPHAEA GSLWRQLFQA GARPCGLGAR DTLRLEAGMR LYGQDMDETV 250 260 270 280 290 300 TPLACGLGWT VAWEPEERDF IGRAALERER IGGSPSKFVG LILEEPGILR SGQKVAVANV 310 320 330 340 350 360 GEGVVTSGGF SPTLRRSIGL ARVPAATGRE CRVEIRGSLK RATVVKPRFV RRSTSLIDIC

References

[1]

"Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath)." Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S., Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E., Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J. , Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L., Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H., Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R., Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A. PLoS Biol. 2:1616-1628(2004) [PubMed: 15383840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33009 / NCIMB 11132 / Bath.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017282 Genomic DNA. Translation: AAU90545.1.
RefSeq	YP_112882.1. NC_002977.6.
3D structure databases
ProteinModelPortal	Q60BW3.
SMR	Q60BW3. Positions 5-360.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3105022.
GenomeReviews	Gene locus MCA0350 in contig AE017282_GR.
KEGG	mca:MCA0350.
NMPDR	fig|243233.4.peg.720.
PATRIC	22604443. VBIMetCap22254_0363.
TIGR	MCA0350.
Phylogenomic databases
HOGENOM	HBG299834.
OMA	HAREASW.
ProtClustDB	PRK00389.
Enzyme and pathway databases
BioCyc	MCAP243233:MCA_0350-MONOMER.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_METCA

Accession

Primary (citable) accession number: Q60BW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program