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Q60BB2

- GLND_METCA

UniProt

Q60BB2 - GLND_METCA

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, MCA0565
Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:MCA0565
OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Taxonomic identifieri243233 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
ProteomesiUP000006821: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 877877Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_0000192744Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243233.MCA0565.

Structurei

3D structure databases

ProteinModelPortaliQ60BB2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini454 – 568115HD
Add
BLAST
Domaini696 – 77883ACT 1
Add
BLAST
Domaini805 – 87773ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 335335UridylyltransferaseUniRule annotation
Add
BLAST
Regioni336 – 695360Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60BB2-1 [UniParc]FASTAAdd to Basket

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MDGPNSDRAH DRHAFDRVAA CKARIQHNTA ELAERFRTGT PVADLIRERT    50
AFIDHLLSEA WDRRIGRGAT DVALVAVGGY GRGELLLHSD VDLLILLDEA 100
APSSRKQDLS DFLRLLWDIG LKPGHSVRSP AECAEAARTD QTIITNLLEG 150
RLLVGSAALW EAVRSETAPE RMWSSAAFFE AKMAEQRIRY SKYHNTAYNL 200
EPNVKEGPGG LRDIQLIGWI IRRHSDARGL QDLVAHGWLT DAEYRELKEA 250
QAFLWRIRFA LHALTGRCED RLLFDYQREL AGLFGYRGET SNEVVEGFMQ 300
DYFRTVTGVE RLNELLLQLF NEAVLHRDDA FSPTPVNDHF QAVNDYLEAV 350
HPAVFREHPL ALLEVFLILQ KNSALEGVRA ATIRLIRQHI HLIDDAFRND 400
PEACRLFMDI LRQPGGVTHQ LRRMNRYGVL AAYLPEFGRV VGRMQYDLFH 450
VYTVDEHTLF VVRNLRRFAL EEFQQENPLC YELFQLIEKP ELLYIAALMH 500
DIAKGSDGDH SEVGERIAEE FCRRHRIGPR ETLLVKWLVR HHLVMSMTAQ 550
RKDLSDPEVI HEFAQIVRNQ NTLNHLYLLT VADIRATNPS LWNSWKGALL 600
QELYTSTSWT LRRGLDTPPD YAEQISAAKD EARTLLQRFG LAEDAITAVW 650
ENIGDDYFLR FLPEEIAWHT TAIAACRPEH LPLVLLRPES LRGSVEVFIY 700
ERNRDFLFAQ TTAVLDQLGL TVLDAKIIAS RQGFALLSFN VLERSGTAPE 750
GLFRLVQICD RLKEALSGGG APPPAVSRLA TRQIRHFTVP TKVFFHDDPQ 800
NRFSILELIA TDRPGLLSKV GQAFMRTGIR LHNAKISTVG SRAEDIFFIT 850
DREDRPLDGE ADRAALRRVL IEFVGDQ 877
Length:877
Mass (Da):99,886
Last modified:November 23, 2004 - v1
Checksum:i54954C07DB9E3AE9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017282 Genomic DNA. Translation: AAU93245.1.
RefSeqiYP_113084.1. NC_002977.6.

Genome annotation databases

EnsemblBacteriaiAAU93245; AAU93245; MCA0565.
GeneIDi3103204.
KEGGimca:MCA0565.
PATRICi22604896. VBIMetCap22254_0581.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017282 Genomic DNA. Translation: AAU93245.1 .
RefSeqi YP_113084.1. NC_002977.6.

3D structure databases

ProteinModelPortali Q60BB2.
ModBasei Search...

Protein-protein interaction databases

STRINGi 243233.MCA0565.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU93245 ; AAU93245 ; MCA0565 .
GeneIDi 3103204.
KEGGi mca:MCA0565.
PATRICi 22604896. VBIMetCap22254_0581.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33009 / NCIMB 11132 / Bath.

Entry informationi

Entry nameiGLND_METCA
AccessioniPrimary (citable) accession number: Q60BB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 23, 2004
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi