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Q60BB2 (GLND_METCA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme

Short name=UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein

Including the following 2 domains:

  1. [Protein-PII] uridylyltransferase
    Short name=PII uridylyltransferase
    Short name=UTase
    EC=2.7.7.59
  2. [Protein-PII]-UMP uridylyl-removing enzyme
    Short name=UR
    EC=3.1.4.-
Gene names
Name:glnD
Ordered Locus Names:MCA0565
OrganismMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]
Taxonomic identifier243233 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus

Protein attributes

Sequence length877 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism By similarity. HAMAP-Rule MF_00277

Catalytic activity

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP-Rule MF_00277

Uridylyl-[protein-PII] + H2O = UMP + [protein-PII]. HAMAP-Rule MF_00277

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00277

Enzyme regulation

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity. HAMAP-Rule MF_00277

Domain

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity. HAMAP-Rule MF_00277

Sequence similarities

Belongs to the GlnD family.

Contains 2 ACT domains.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 877877Bifunctional uridylyltransferase/uridylyl-removing enzyme HAMAP-Rule MF_00277
PRO_0000192744

Regions

Domain454 – 568115HD
Domain696 – 77883ACT 1
Domain805 – 87773ACT 2
Region1 – 335335Uridylyltransferase HAMAP-Rule MF_00277
Region336 – 695360Uridylyl-removing HAMAP-Rule MF_00277

Sequences

Sequence LengthMass (Da)Tools
Q60BB2 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 54954C07DB9E3AE9

FASTA87799,886
        10         20         30         40         50         60 
MDGPNSDRAH DRHAFDRVAA CKARIQHNTA ELAERFRTGT PVADLIRERT AFIDHLLSEA 

        70         80         90        100        110        120 
WDRRIGRGAT DVALVAVGGY GRGELLLHSD VDLLILLDEA APSSRKQDLS DFLRLLWDIG 

       130        140        150        160        170        180 
LKPGHSVRSP AECAEAARTD QTIITNLLEG RLLVGSAALW EAVRSETAPE RMWSSAAFFE 

       190        200        210        220        230        240 
AKMAEQRIRY SKYHNTAYNL EPNVKEGPGG LRDIQLIGWI IRRHSDARGL QDLVAHGWLT 

       250        260        270        280        290        300 
DAEYRELKEA QAFLWRIRFA LHALTGRCED RLLFDYQREL AGLFGYRGET SNEVVEGFMQ 

       310        320        330        340        350        360 
DYFRTVTGVE RLNELLLQLF NEAVLHRDDA FSPTPVNDHF QAVNDYLEAV HPAVFREHPL 

       370        380        390        400        410        420 
ALLEVFLILQ KNSALEGVRA ATIRLIRQHI HLIDDAFRND PEACRLFMDI LRQPGGVTHQ 

       430        440        450        460        470        480 
LRRMNRYGVL AAYLPEFGRV VGRMQYDLFH VYTVDEHTLF VVRNLRRFAL EEFQQENPLC 

       490        500        510        520        530        540 
YELFQLIEKP ELLYIAALMH DIAKGSDGDH SEVGERIAEE FCRRHRIGPR ETLLVKWLVR 

       550        560        570        580        590        600 
HHLVMSMTAQ RKDLSDPEVI HEFAQIVRNQ NTLNHLYLLT VADIRATNPS LWNSWKGALL 

       610        620        630        640        650        660 
QELYTSTSWT LRRGLDTPPD YAEQISAAKD EARTLLQRFG LAEDAITAVW ENIGDDYFLR 

       670        680        690        700        710        720 
FLPEEIAWHT TAIAACRPEH LPLVLLRPES LRGSVEVFIY ERNRDFLFAQ TTAVLDQLGL 

       730        740        750        760        770        780 
TVLDAKIIAS RQGFALLSFN VLERSGTAPE GLFRLVQICD RLKEALSGGG APPPAVSRLA 

       790        800        810        820        830        840 
TRQIRHFTVP TKVFFHDDPQ NRFSILELIA TDRPGLLSKV GQAFMRTGIR LHNAKISTVG 

       850        860        870 
SRAEDIFFIT DREDRPLDGE ADRAALRRVL IEFVGDQ 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017282 Genomic DNA. Translation: AAU93245.1.
RefSeqYP_113084.1. NC_002977.6.

3D structure databases

ProteinModelPortalQ60BB2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243233.MCA0565.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU93245; AAU93245; MCA0565.
GeneID3103204.
KEGGmca:MCA0565.
PATRIC22604896. VBIMetCap22254_0581.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2844.
HOGENOMHOG000261778.
KOK00990.
OMAHHLLMSV.
OrthoDBEOG6CCH44.

Family and domain databases

Gene3D1.10.3210.10. 1 hit.
HAMAPMF_00277. PII_uridylyl_transf.
InterProIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFPIRSF006288. PII_uridyltransf. 1 hit.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsTIGR01693. UTase_glnD. 1 hit.
PROSITEPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLND_METCA
AccessionPrimary (citable) accession number: Q60BB2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 23, 2004
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families