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Q60BB2

- GLND_METCA

UniProt

Q60BB2 - GLND_METCA

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:MCA0565
OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Taxonomic identifieri243233 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
ProteomesiUP000006821: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 877877Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192744Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243233.MCA0565.

Structurei

3D structure databases

ProteinModelPortaliQ60BB2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini454 – 568115HDUniRule annotationAdd
BLAST
Domaini696 – 77883ACT 1UniRule annotationAdd
BLAST
Domaini805 – 87773ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 335335UridylyltransferaseAdd
BLAST
Regioni336 – 695360Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60BB2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDGPNSDRAH DRHAFDRVAA CKARIQHNTA ELAERFRTGT PVADLIRERT
60 70 80 90 100
AFIDHLLSEA WDRRIGRGAT DVALVAVGGY GRGELLLHSD VDLLILLDEA
110 120 130 140 150
APSSRKQDLS DFLRLLWDIG LKPGHSVRSP AECAEAARTD QTIITNLLEG
160 170 180 190 200
RLLVGSAALW EAVRSETAPE RMWSSAAFFE AKMAEQRIRY SKYHNTAYNL
210 220 230 240 250
EPNVKEGPGG LRDIQLIGWI IRRHSDARGL QDLVAHGWLT DAEYRELKEA
260 270 280 290 300
QAFLWRIRFA LHALTGRCED RLLFDYQREL AGLFGYRGET SNEVVEGFMQ
310 320 330 340 350
DYFRTVTGVE RLNELLLQLF NEAVLHRDDA FSPTPVNDHF QAVNDYLEAV
360 370 380 390 400
HPAVFREHPL ALLEVFLILQ KNSALEGVRA ATIRLIRQHI HLIDDAFRND
410 420 430 440 450
PEACRLFMDI LRQPGGVTHQ LRRMNRYGVL AAYLPEFGRV VGRMQYDLFH
460 470 480 490 500
VYTVDEHTLF VVRNLRRFAL EEFQQENPLC YELFQLIEKP ELLYIAALMH
510 520 530 540 550
DIAKGSDGDH SEVGERIAEE FCRRHRIGPR ETLLVKWLVR HHLVMSMTAQ
560 570 580 590 600
RKDLSDPEVI HEFAQIVRNQ NTLNHLYLLT VADIRATNPS LWNSWKGALL
610 620 630 640 650
QELYTSTSWT LRRGLDTPPD YAEQISAAKD EARTLLQRFG LAEDAITAVW
660 670 680 690 700
ENIGDDYFLR FLPEEIAWHT TAIAACRPEH LPLVLLRPES LRGSVEVFIY
710 720 730 740 750
ERNRDFLFAQ TTAVLDQLGL TVLDAKIIAS RQGFALLSFN VLERSGTAPE
760 770 780 790 800
GLFRLVQICD RLKEALSGGG APPPAVSRLA TRQIRHFTVP TKVFFHDDPQ
810 820 830 840 850
NRFSILELIA TDRPGLLSKV GQAFMRTGIR LHNAKISTVG SRAEDIFFIT
860 870
DREDRPLDGE ADRAALRRVL IEFVGDQ
Length:877
Mass (Da):99,886
Last modified:November 23, 2004 - v1
Checksum:i54954C07DB9E3AE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017282 Genomic DNA. Translation: AAU93245.1.
RefSeqiWP_010959913.1. NC_002977.6.
YP_113084.1. NC_002977.6.

Genome annotation databases

EnsemblBacteriaiAAU93245; AAU93245; MCA0565.
GeneIDi3103204.
KEGGimca:MCA0565.
PATRICi22604896. VBIMetCap22254_0581.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017282 Genomic DNA. Translation: AAU93245.1 .
RefSeqi WP_010959913.1. NC_002977.6.
YP_113084.1. NC_002977.6.

3D structure databases

ProteinModelPortali Q60BB2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243233.MCA0565.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU93245 ; AAU93245 ; MCA0565 .
GeneIDi 3103204.
KEGGi mca:MCA0565.
PATRICi 22604896. VBIMetCap22254_0581.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33009 / NCIMB 11132 / Bath.

Entry informationi

Entry nameiGLND_METCA
AccessioniPrimary (citable) accession number: Q60BB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 23, 2004
Last modified: November 26, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3