Malate dehydrogenase - Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	MCA0610

Organism

Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]

Taxonomic identifier

243233 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus ›

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01517
Catalytic activity	(S)-malate + NAD⁺ = oxaloacetate + NADH. HAMAP-Rule MF_01517
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 325

325

Malate dehydrogenase HAMAP-Rule MF_01517

PRO_0000113376

Regions

Nucleotide binding

11 – 17

7

NAD By similarity

Nucleotide binding

129 – 131

3

NAD By similarity

Sites

Active site

187

1

Proton acceptor By similarity

Binding site

92

1

Substrate By similarity

Binding site

98

1

Substrate By similarity

Binding site

105

1

NAD By similarity

Binding site

112

1

NAD By similarity

Binding site

131

1

Substrate By similarity

Binding site

162

1

Substrate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q60B71 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 3001033571878896
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FASTA

325

35,244

        10         20         30         40         50         60 
MKTPVHVAVT GAAGQIAYSL LFRIAVGDLF GPHQPVILKL LDVPSAERVL EGVAMELDDC 

        70         80         90        100        110        120 
ASPLLQEIEV SSDPAEVFDG AEAVFMLGAT PRGPGMERRD LLQVNADIFS AQGRALNESA 

       130        140        150        160        170        180 
SRRVKILVVG NPANTNALIA QRNAPDLAPG CFSAMTRLDH NRATSLLARH CGCNVAEISR 

       190        200        210        220        230        240 
VVIWGNHSPT QYPDLHHARV KGKPALSLVD PAWYVETFIP TVQQRGASVI AIRGKSSAAS 

       250        260        270        280        290        300 
AANAALDHMR SWFLGTPKDD WVSMTVSSDG SYGIAEGLMF SFPVTIENGR FRIVQDLPLD 

       310        320 
TFSRERLRLT EVELLEERAM VSHLL

« Hide

References

[1]

"Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath)."
Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S., Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E., Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J.

Eisen J.A.
PLoS Biol. 2:1616-1628(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33009 / NCIMB 11132 / Bath.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017282 Genomic DNA. Translation: AAU93114.1.
RefSeq	YP_113126.1. NC_002977.6.
3D structure databases
ProteinModelPortal	Q60B71.
SMR	Q60B71. Positions 1-324.
ModBase	Search...
Protein-protein interaction databases
STRING	243233.MCA0610.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU93114; AAU93114; MCA0610.
GeneID	3103776.
KEGG	mca:MCA0610.
PATRIC	22604990. VBIMetCap22254_0627.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000220953.
KO	K00024.
OMA	EWALLIG.
ProtClustDB	PRK05442.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_01517. Malate_dehydrog_2.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010945. Malate_DH_type2. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR23382. PTHR23382. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_METCA

Accession

Primary (citable) accession number: Q60B71

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents