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Protein

Malate dehydrogenase

Gene

mdh

Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateUniRule annotation1
Binding sitei98SubstrateUniRule annotation1
Binding sitei105NADUniRule annotation1
Binding sitei112NADUniRule annotation1
Binding sitei131SubstrateUniRule annotation1
Binding sitei162SubstrateUniRule annotation1
Active sitei187Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 17NADUniRule annotation7
Nucleotide bindingi129 – 131NADUniRule annotation3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: UniProtKB-HAMAP
  • malate dehydrogenase activity Source: JCVI

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • malate metabolic process Source: InterPro
  • tricarboxylic acid cycle Source: JCVI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:MCA0610
OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Taxonomic identifieri243233 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
Proteomesi
  • UP000006821 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001133761 – 325Malate dehydrogenaseAdd BLAST325

Interactioni

Protein-protein interaction databases

STRINGi243233.MCA0610.

Structurei

3D structure databases

ProteinModelPortaliQ60B71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D9Z. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000220953.
KOiK00024.
OMAiAFSQECI.
OrthoDBiPOG091H03R4.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60B71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTPVHVAVT GAAGQIAYSL LFRIAVGDLF GPHQPVILKL LDVPSAERVL
60 70 80 90 100
EGVAMELDDC ASPLLQEIEV SSDPAEVFDG AEAVFMLGAT PRGPGMERRD
110 120 130 140 150
LLQVNADIFS AQGRALNESA SRRVKILVVG NPANTNALIA QRNAPDLAPG
160 170 180 190 200
CFSAMTRLDH NRATSLLARH CGCNVAEISR VVIWGNHSPT QYPDLHHARV
210 220 230 240 250
KGKPALSLVD PAWYVETFIP TVQQRGASVI AIRGKSSAAS AANAALDHMR
260 270 280 290 300
SWFLGTPKDD WVSMTVSSDG SYGIAEGLMF SFPVTIENGR FRIVQDLPLD
310 320
TFSRERLRLT EVELLEERAM VSHLL
Length:325
Mass (Da):35,244
Last modified:November 23, 2004 - v1
Checksum:i3001033571878896
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017282 Genomic DNA. Translation: AAU93114.1.
RefSeqiWP_010959955.1. NC_002977.6.

Genome annotation databases

EnsemblBacteriaiAAU93114; AAU93114; MCA0610.
KEGGimca:MCA0610.
PATRICi22604990. VBIMetCap22254_0627.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017282 Genomic DNA. Translation: AAU93114.1.
RefSeqiWP_010959955.1. NC_002977.6.

3D structure databases

ProteinModelPortaliQ60B71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243233.MCA0610.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU93114; AAU93114; MCA0610.
KEGGimca:MCA0610.
PATRICi22604990. VBIMetCap22254_0627.

Phylogenomic databases

eggNOGiENOG4105D9Z. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000220953.
KOiK00024.
OMAiAFSQECI.
OrthoDBiPOG091H03R4.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_METCA
AccessioniPrimary (citable) accession number: Q60B71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: November 23, 2004
Last modified: October 5, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.