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Q60AP9 (MTNC_METCA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Enolase-phosphatase E1
EC=3.1.3.77
Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Gene names
Name:mtnC
Ordered Locus Names:MCA0797
OrganismMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]
Taxonomic identifier243233 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity. HAMAP MF_01681

Catalytic activity

5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate. HAMAP MF_01681

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01681

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. HAMAP MF_01681

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.

Subunit structure

Monomer By similarity. HAMAP MF_01681

Sequence similarities

Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227Enolase-phosphatase E1 HAMAP MF_01681
PRO_0000357382

Sequences

Sequence LengthMass (Da)Tools
Q60AP9 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 43CD11C20F73C07E

FASTA22725,246
        10         20         30         40         50         60 
MIRAILTDIE GTTSSLSFVK ETLFPYARAR MADFVRGHAR DATVQALLAD AKAAAGDPSM 

        70         80         90        100        110        120 
DDEHVIARLV RWIDEDRKIT PLKALQGLIW EEGYRNRDFF GHVYDDAVRR LKAWHEQGIS 

       130        140        150        160        170        180 
LYVFSSGSVH AQRLLFGHTA AGDLQPLFSG YFDTRIGAKQ EPAAYSAIAR ELNLPPSEIL 

       190        200        210        220 
FLSDIEAELD AAREAGYKTF MLVREGGAKA SHHPQGADFD AIRPELL

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017282 Genomic DNA. Translation: AAU92902.1.
RefSeqYP_113298.1. NC_002977.6.

3D structure databases

HSSPHSSP built from PDB template 2G80 based on UniProtKB P32626.
ProteinModelPortalQ60AP9.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3103415.
GenomeReviewsGene locus MCA0797 in contig AE017282_GR.
KEGGmca:MCA0797.
NMPDRfig|243233.4.peg.1588.
PATRIC22605392. VBIMetCap22254_0819.
TIGRMCA0797.

Phylogenomic databases

HOGENOMHBG294242.
OMATTDLNFI.
ProtClustDBCLSK866737.

Enzyme and pathway databases

BioCycMCAP243233:MCA_0797-MONOMER.

Family and domain databases

HAMAPMF_01681. Salvage_MtnC.
[Tree]
InterProIPR005834. Dehalogen-like_hydro.
IPR023943. Enolase-phosphatase_E1.
IPR010041. Enolase_ppase.
IPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA_v1.
[Graphical view]
Gene3DG3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
KOK09880.
PfamPF00702. Hydrolase. 1 hit.
[Graphical view]
SUPFAMSSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01691. Enolase-ppase. 1 hit.
TIGR01549. HAD-SF-IA-v1. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMTNC_METCA
AccessionPrimary (citable) accession number: Q60AP9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: November 23, 2004
Last modified: January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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