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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109ATPUniRule annotation1
Active sitei319Tele-AMP-histidine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi293 – 297ATPUniRule annotation5

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • RNA-3'-phosphate cyclase activity Source: JCVI

GO - Biological processi

  • RNA processing Source: JCVI

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMCAP243233:G1G0X-1212-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:MCA1212
OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Taxonomic identifieri243233 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
Proteomesi
  • UP000006821 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002647961 – 361RNA 3'-terminal phosphate cyclaseAdd BLAST361

Proteomic databases

PRIDEiQ609M1

Interactioni

Protein-protein interaction databases

STRINGi243233.MCA1212

Structurei

3D structure databases

ProteinModelPortaliQ609M1
SMRiQ609M1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKX Bacteria
COG0430 LUCA
HOGENOMiHOG000015264
KOiK01974
OMAiANRPKPG
OrthoDBiPOG091H0UB7

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF52913 SSF52913, 1 hit
SSF55205 SSF55205, 1 hit
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit

Sequencei

Sequence statusi: Complete.

Q609M1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLRIDGSY GEGGGQIIRT ALSLAALTGT AVRLENIRAR RRKPGLAAQH
60 70 80 90 100
LTAVKAVALL CDARIGGAEL GSQTLEFIPG RPVRAGDYTL DVGEAREGGS
110 120 130 140 150
AGAVMLVLQT VLPPLALAEG ASTVSLRGGT HVPMSPPFDY VREVWLPTLA
160 170 180 190 200
RMGVRAELSL VRSGWYPVGK GEVRLVVAGG RRSLEALKAR HRGTLQAVTG
210 220 230 240 250
RALAANLPAH IPERMAARAR AVLADAGIAA AIEPAVLQAA CAGAGLFLTA
260 270 280 290 300
RYDHCLAGFT ALGRRGKSAE RVAEEACGAL LAHHRSGAAL DQHLADQIVL
310 320 330 340 350
PAALCREESL FSVERITPHL TTNAWVVDRF GLARVDVRLA ECGTGLVRIH
360
GNPAGVCHAH P
Length:361
Mass (Da):37,839
Last modified:November 23, 2004 - v1
Checksum:iFAAED1D5532FDED1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017282 Genomic DNA Translation: AAU92541.1
RefSeqiWP_010960498.1, NC_002977.6

Genome annotation databases

EnsemblBacteriaiAAU92541; AAU92541; MCA1212
KEGGimca:MCA1212

Similar proteinsi

Entry informationi

Entry nameiRTCA_METCA
AccessioniPrimary (citable) accession number: Q609M1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 23, 2004
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health