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Q609M1 (RTCA_METCA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4
Gene names
Name:rtcA
Ordered Locus Names:MCA1212
OrganismMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]
Taxonomic identifier243233 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus ›

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP-Rule MF_00200

Catalytic activity

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP-Rule MF_00200

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00200.

Sequence similarities

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-3'-phosphate cyclase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 361361RNA 3'-terminal phosphate cyclase HAMAP-Rule MF_00200
PRO_0000264796

Regions

Nucleotide binding293 – 2975ATP By similarity

Sites

Active site3191Tele-AMP-histidine intermediate By similarity
Binding site1091ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q609M1 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: FAAED1D5532FDED1

FASTA36137,839
        10         20         30         40         50         60 
MTLLRIDGSY GEGGGQIIRT ALSLAALTGT AVRLENIRAR RRKPGLAAQH LTAVKAVALL 

        70         80         90        100        110        120 
CDARIGGAEL GSQTLEFIPG RPVRAGDYTL DVGEAREGGS AGAVMLVLQT VLPPLALAEG 

       130        140        150        160        170        180 
ASTVSLRGGT HVPMSPPFDY VREVWLPTLA RMGVRAELSL VRSGWYPVGK GEVRLVVAGG 

       190        200        210        220        230        240 
RRSLEALKAR HRGTLQAVTG RALAANLPAH IPERMAARAR AVLADAGIAA AIEPAVLQAA 

       250        260        270        280        290        300 
CAGAGLFLTA RYDHCLAGFT ALGRRGKSAE RVAEEACGAL LAHHRSGAAL DQHLADQIVL 

       310        320        330        340        350        360 
PAALCREESL FSVERITPHL TTNAWVVDRF GLARVDVRLA ECGTGLVRIH GNPAGVCHAH 


P

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017282 Genomic DNA. Translation: AAU92541.1.
RefSeqYP_113676.1. NC_002977.6.

3D structure databases

HSSPHSSP built from PDB template 1QMH based on UniProtKB P46849.
ProteinModelPortalQ609M1.
ModBaseSearch...

Protein-protein interaction databases

STRING243233.MCA1212.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU92541; AAU92541; MCA1212.
GeneID3102796.
KEGGmca:MCA1212.
PATRIC22606260. VBIMetCap22254_1246.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0430.
HOGENOMHOG000015264.
KOK01974.
OMARRGHYPK.
ProtClustDBPRK04204.

Family and domain databases

Gene3D3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPMF_00200. RTC.
InterProIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERPTHR11096. PTHR11096. 1 hit.
PfamPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMSSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMsTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEPS01287. RTC. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRTCA_METCA
AccessionPrimary (citable) accession number: Q609M1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 23, 2004
Last modified: May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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