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Q60996 (2A5G_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
Alternative name(s):
PP2A B subunit isoform B'-alpha-3
PP2A B subunit isoform B'-gamma
PP2A B subunit isoform B56-gamma
PP2A B subunit isoform PR61-gamma
PP2A B subunit isoform R5-gamma
Gene names
Name:Ppp2r5c
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation By similarity.

Subunit structure

PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGOL1 By similarity. Interacts with SGOL1; the interaction is direct. May interact with TP53 By similarity. Interacts with IER3 and/or ERK kinases; regulates ERK dephosphorylation By similarity.

Subcellular location

Nucleus By similarity. Chromosomecentromere By similarity.

Tissue specificity

Highest levels in heart, liver and brain. Lower levels in skeletal muscle, spleen, kidney and lung. Isoform 4 is testis-specific. Ref.5

Sequence similarities

Belongs to the phosphatase 2A regulatory subunit B56 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PPP2R1AP301532EBI-1369292,EBI-302388From a different organism.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: Q60996-1)

Also known as: Gamma-2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: Q60996-2)

Also known as: Gamma-1;

The sequence of this isoform differs from the canonical sequence as follows:
     443-452: YAVYSQASAV → VLKKRVTREC
     453-524: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q60996-3)

Also known as: Gamma-3;

The sequence of this isoform differs from the canonical sequence as follows:
     442-480: Missing.
Isoform 4 (identifier: Q60996-4)

Also known as: Gamma-4;

The sequence of this isoform differs from the canonical sequence as follows:
     482-496: AQKELKKDRPLVRRK → LVGRKAMTATQVRKV
     497-524: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 524524Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
PRO_0000071458

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Natural variations

Alternative sequence442 – 48039Missing in isoform 3.
VSP_014718
Alternative sequence443 – 45210YAVYSQASAV → VLKKRVTREC in isoform 1.
VSP_014719
Alternative sequence453 – 52472Missing in isoform 1.
VSP_014720
Alternative sequence482 – 49615AQKEL…LVRRK → LVGRKAMTATQVRKV in isoform 4.
VSP_038641
Alternative sequence497 – 52428Missing in isoform 4.
VSP_038642

Experimental info

Sequence conflict17 – 193SSN → APT in BAB32447. Ref.2
Sequence conflict17 – 193SSN → APT in BAB32448. Ref.2
Sequence conflict321D → N in AAB70857. Ref.4
Sequence conflict721A → S Ref.3
Sequence conflict721A → S Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 (Gamma-2) [UniParc].

Last modified July 19, 2005. Version 2.
Checksum: A22DFD34A1FFBD01

FASTA52460,824
        10         20         30         40         50         60 
MLTCNKAGSG MVVDAASSNG PFQPVALLHI RDVPPADQEK LFIQKLRQCC VLFDFVSDPL 

        70         80         90        100        110        120 
SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEAVHMFA VNMFRTLPPS SNPTGAEFDP 

       130        140        150        160        170        180 
EEDEPTLEAA WPHLQLVYEF FLRFLESPDF QPNIAKKYID QKFVLQLLEL FDSEDPRERD 

       190        200        210        220        230        240 
FLKTTLHRIY GKFLGLRAYI RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK 

       250        260        270        280        290        300 
EEHKIFLLKV LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK 

       310        320        330        340        350        360 
EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW NNEYIMSLIS 

       370        380        390        400        410        420 
DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM NQKLFDDCTQ QFKAEKLKEK 

       430        440        450        460        470        480 
LKMKEREEAW VKIENLAKAN PQYAVYSQAS AVSIPVAMET DGPQFEDVQM LKKTVSDEAR 

       490        500        510        520 
QAQKELKKDR PLVRRKSELP QDPHTEKALE AHCRASELLS QDGR 

« Hide

Isoform 1 (Gamma-1) [UniParc].

Checksum: DFD00AF40F422088
Show »

FASTA45252,818
Isoform 3 (Gamma-3) [UniParc].

Checksum: E5BE84FF6E3E01C7
Show »

FASTA48556,552
Isoform 4 (Gamma-4) [UniParc].

Checksum: 8D2004773C96E41E
Show »

FASTA49657,517

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary tumor.
[2]"A truncated isoform of the protein phosphatase 2A B56gamma regulatory subunit may promote genetic instability and cause tumor progression."
Ito A., Koma Y., Watabe K., Nagano T., Endo Y., Nojima H., Kitamura Y.
Am. J. Pathol. 162:81-91(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-524 (ISOFORMS 2 AND 3).
[3]"Identification of a novel protein phosphatase 2A regulatory subunit highly expressed in muscle."
Tehrani M.A., Mumby M.C., Kamibayashi C.
J. Biol. Chem. 271:5164-5170(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-524 (ISOFORM 1).
Tissue: T-cell.
[4]"Identification by differential display of annexin-VI, a gene differentially expressed during melanoma progression."
Francia G., Mitchell S.D., Moss S.E., Hanby A.M., Marshall J.F., Hart I.R.
Cancer Res. 56:3855-3858(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-524 (ISOFORM 1).
Strain: C57BL/6.
[5]"Expression of the B56delta subunit of protein phosphatase 2A and Mea1 in mouse spermatogenesis. Identification of a new B56gamma subunit (B56gamma4) specifically expressed in testis."
Ortega-Lazaro J.C., del Mazo J.
Cytogenet. Genome Res. 103:345-351(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 326-524 (ISOFORM 4), TISSUE SPECIFICITY.
Strain: CD-1.
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC003979 mRNA. Translation: AAH03979.1.
AB055635 mRNA. Translation: BAB32447.1.
AB055636 mRNA. Translation: BAB32448.1.
U37353 mRNA. Translation: AAC52435.1.
U59418 mRNA. Translation: AAB70857.1.
AY389498 mRNA. Translation: AAR26474.1.
RefSeqNP_001074926.1. NM_001081457.2.
NP_001074927.1. NM_001081458.2.
NP_036153.2. NM_012023.3.
UniGeneMm.240396.
Mm.458056.
Mm.490988.

3D structure databases

ProteinModelPortalQ60996.
SMRQ60996. Positions 34-436.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-24181N.
IntActQ60996. 1 interaction.

PTM databases

PhosphoSiteQ60996.

Proteomic databases

PaxDbQ60996.
PRIDEQ60996.

Protocols and materials databases

DNASU26931.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000084985; ENSMUSP00000082053; ENSMUSG00000017843. [Q60996-1]
ENSMUST00000109832; ENSMUSP00000105458; ENSMUSG00000017843. [Q60996-3]
GeneID26931.
KEGGmmu:26931.
UCSCuc007pbk.1. mouse. [Q60996-2]
uc007pbl.1. mouse. [Q60996-1]
uc007pbm.1. mouse. [Q60996-3]

Organism-specific databases

CTD5527.
MGIMGI:1349475. Ppp2r5c.

Phylogenomic databases

eggNOGNOG264925.
GeneTreeENSGT00550000074525.
HOGENOMHOG000067326.
HOVERGENHBG000009.
KOK11584.
OMANALEAHC.
OrthoDBEOG7C2R2S.
PhylomeDBQ60996.
TreeFamTF105556.

Gene expression databases

BgeeQ60996.
GenevestigatorQ60996.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR002554. PP2A_B56.
[Graphical view]
PANTHERPTHR10257. PTHR10257. 1 hit.
PfamPF01603. B56. 1 hit.
[Graphical view]
PIRSFPIRSF028043. PP2A_B56. 1 hit.
SUPFAMSSF48371. SSF48371. 1 hit.
ProtoNetSearch...

Other

ChiTaRSPPP2R5C. mouse.
NextBio304827.
PROQ60996.
SOURCESearch...

Entry information

Entry name2A5G_MOUSE
AccessionPrimary (citable) accession number: Q60996
Secondary accession number(s): O35708 expand/collapse secondary AC list , Q6TQF7, Q99KW8, Q99N67, Q99N68
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 19, 2005
Last modified: April 16, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot