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Q60996

- 2A5G_MOUSE

UniProt

Q60996 - 2A5G_MOUSE

Protein

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform

Gene

Ppp2r5c

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein phosphatase type 2A regulator activity Source: InterPro

    GO - Biological processi

    1. DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Ensembl
    2. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: Ensembl
    3. negative regulation of cell proliferation Source: Ensembl
    4. proteasome-mediated ubiquitin-dependent protein catabolic process Source: MGI

    Enzyme and pathway databases

    ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_203336. Degradation of beta-catenin by the destruction complex.
    REACT_207679. Separation of Sister Chromatids.
    REACT_216784. disassembly of the destruction complex and recruitment of AXIN to the membrane.
    REACT_218396. Beta-catenin phosphorylation cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
    Alternative name(s):
    PP2A B subunit isoform B'-alpha-3
    PP2A B subunit isoform B'-gamma
    PP2A B subunit isoform B56-gamma
    PP2A B subunit isoform PR61-gamma
    PP2A B subunit isoform R5-gamma
    Gene namesi
    Name:Ppp2r5c
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1349475. Ppp2r5c.

    Subcellular locationi

    Nucleus By similarity. Chromosomecentromere By similarity

    GO - Cellular componenti

    1. chromosome, centromeric region Source: UniProtKB
    2. nucleus Source: MGI
    3. protein phosphatase type 2A complex Source: UniProtKB

    Keywords - Cellular componenti

    Centromere, Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 524524Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoformPRO_0000071458Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ60996.
    PaxDbiQ60996.
    PRIDEiQ60996.

    PTM databases

    PhosphoSiteiQ60996.

    Expressioni

    Tissue specificityi

    Highest levels in heart, liver and brain. Lower levels in skeletal muscle, spleen, kidney and lung. Isoform 4 is testis-specific.1 Publication

    Gene expression databases

    BgeeiQ60996.
    GenevestigatoriQ60996.

    Interactioni

    Subunit structurei

    PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGOL1 By similarity. Interacts with SGOL1; the interaction is direct. May interact with TP53 By similarity. Interacts with IER3 and/or ERK kinases; regulates ERK dephosphorylation By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PPP2R1AP301532EBI-1369292,EBI-302388From a different organism.

    Protein-protein interaction databases

    BioGridi205070. 1 interaction.
    DIPiDIP-24181N.
    IntActiQ60996. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ60996.
    SMRiQ60996. Positions 34-436.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG264925.
    GeneTreeiENSGT00550000074525.
    HOGENOMiHOG000067326.
    HOVERGENiHBG000009.
    KOiK11584.
    OMAiESHCRAD.
    OrthoDBiEOG7C2R2S.
    PhylomeDBiQ60996.
    TreeFamiTF105556.

    Family and domain databases

    InterProiIPR016024. ARM-type_fold.
    IPR002554. PP2A_B56.
    [Graphical view]
    PANTHERiPTHR10257. PTHR10257. 1 hit.
    PfamiPF01603. B56. 1 hit.
    [Graphical view]
    PIRSFiPIRSF028043. PP2A_B56. 1 hit.
    SUPFAMiSSF48371. SSF48371. 1 hit.

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 2 (identifier: Q60996-1) [UniParc]FASTAAdd to Basket

    Also known as: Gamma-2

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLTCNKAGSG MVVDAASSNG PFQPVALLHI RDVPPADQEK LFIQKLRQCC    50
    VLFDFVSDPL SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEAVHMFA 100
    VNMFRTLPPS SNPTGAEFDP EEDEPTLEAA WPHLQLVYEF FLRFLESPDF 150
    QPNIAKKYID QKFVLQLLEL FDSEDPRERD FLKTTLHRIY GKFLGLRAYI 200
    RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK EEHKIFLLKV 250
    LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK 300
    EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW 350
    NNEYIMSLIS DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM 400
    NQKLFDDCTQ QFKAEKLKEK LKMKEREEAW VKIENLAKAN PQYAVYSQAS 450
    AVSIPVAMET DGPQFEDVQM LKKTVSDEAR QAQKELKKDR PLVRRKSELP 500
    QDPHTEKALE AHCRASELLS QDGR 524
    Length:524
    Mass (Da):60,824
    Last modified:July 19, 2005 - v2
    Checksum:iA22DFD34A1FFBD01
    GO
    Isoform 1 (identifier: Q60996-2) [UniParc]FASTAAdd to Basket

    Also known as: Gamma-1

    The sequence of this isoform differs from the canonical sequence as follows:
         443-452: YAVYSQASAV → VLKKRVTREC
         453-524: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:452
    Mass (Da):52,818
    Checksum:iDFD00AF40F422088
    GO
    Isoform 3 (identifier: Q60996-3) [UniParc]FASTAAdd to Basket

    Also known as: Gamma-3

    The sequence of this isoform differs from the canonical sequence as follows:
         442-480: Missing.

    Show »
    Length:485
    Mass (Da):56,552
    Checksum:iE5BE84FF6E3E01C7
    GO
    Isoform 4 (identifier: Q60996-4) [UniParc]FASTAAdd to Basket

    Also known as: Gamma-4

    The sequence of this isoform differs from the canonical sequence as follows:
         482-496: AQKELKKDRPLVRRK → LVGRKAMTATQVRKV
         497-524: Missing.

    Show »
    Length:496
    Mass (Da):57,517
    Checksum:i8D2004773C96E41E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 193SSN → APT in BAB32447. (PubMed:12507892)Curated
    Sequence conflicti17 – 193SSN → APT in BAB32448. (PubMed:12507892)Curated
    Sequence conflicti32 – 321D → N in AAB70857. (PubMed:8752144)Curated
    Sequence conflicti72 – 721A → S(PubMed:8617797)Curated
    Sequence conflicti72 – 721A → S(PubMed:8752144)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei442 – 48039Missing in isoform 3. 1 PublicationVSP_014718Add
    BLAST
    Alternative sequencei443 – 45210YAVYSQASAV → VLKKRVTREC in isoform 1. 3 PublicationsVSP_014719
    Alternative sequencei453 – 52472Missing in isoform 1. 3 PublicationsVSP_014720Add
    BLAST
    Alternative sequencei482 – 49615AQKEL…LVRRK → LVGRKAMTATQVRKV in isoform 4. 1 PublicationVSP_038641Add
    BLAST
    Alternative sequencei497 – 52428Missing in isoform 4. 1 PublicationVSP_038642Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC003979 mRNA. Translation: AAH03979.1.
    AB055635 mRNA. Translation: BAB32447.1.
    AB055636 mRNA. Translation: BAB32448.1.
    U37353 mRNA. Translation: AAC52435.1.
    U59418 mRNA. Translation: AAB70857.1.
    AY389498 mRNA. Translation: AAR26474.1.
    CCDSiCCDS36557.1. [Q60996-1]
    CCDS36558.1. [Q60996-3]
    RefSeqiNP_001074926.1. NM_001081457.2. [Q60996-3]
    NP_001074927.1. NM_001081458.2. [Q60996-2]
    NP_036153.2. NM_012023.3. [Q60996-1]
    UniGeneiMm.240396.
    Mm.458056.
    Mm.490988.

    Genome annotation databases

    EnsembliENSMUST00000084985; ENSMUSP00000082053; ENSMUSG00000017843. [Q60996-1]
    ENSMUST00000109832; ENSMUSP00000105458; ENSMUSG00000017843. [Q60996-3]
    GeneIDi26931.
    KEGGimmu:26931.
    UCSCiuc007pbk.1. mouse. [Q60996-2]
    uc007pbl.1. mouse. [Q60996-1]
    uc007pbm.1. mouse. [Q60996-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC003979 mRNA. Translation: AAH03979.1 .
    AB055635 mRNA. Translation: BAB32447.1 .
    AB055636 mRNA. Translation: BAB32448.1 .
    U37353 mRNA. Translation: AAC52435.1 .
    U59418 mRNA. Translation: AAB70857.1 .
    AY389498 mRNA. Translation: AAR26474.1 .
    CCDSi CCDS36557.1. [Q60996-1 ]
    CCDS36558.1. [Q60996-3 ]
    RefSeqi NP_001074926.1. NM_001081457.2. [Q60996-3 ]
    NP_001074927.1. NM_001081458.2. [Q60996-2 ]
    NP_036153.2. NM_012023.3. [Q60996-1 ]
    UniGenei Mm.240396.
    Mm.458056.
    Mm.490988.

    3D structure databases

    ProteinModelPortali Q60996.
    SMRi Q60996. Positions 34-436.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 205070. 1 interaction.
    DIPi DIP-24181N.
    IntActi Q60996. 1 interaction.

    PTM databases

    PhosphoSitei Q60996.

    Proteomic databases

    MaxQBi Q60996.
    PaxDbi Q60996.
    PRIDEi Q60996.

    Protocols and materials databases

    DNASUi 26931.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000084985 ; ENSMUSP00000082053 ; ENSMUSG00000017843 . [Q60996-1 ]
    ENSMUST00000109832 ; ENSMUSP00000105458 ; ENSMUSG00000017843 . [Q60996-3 ]
    GeneIDi 26931.
    KEGGi mmu:26931.
    UCSCi uc007pbk.1. mouse. [Q60996-2 ]
    uc007pbl.1. mouse. [Q60996-1 ]
    uc007pbm.1. mouse. [Q60996-3 ]

    Organism-specific databases

    CTDi 5527.
    MGIi MGI:1349475. Ppp2r5c.

    Phylogenomic databases

    eggNOGi NOG264925.
    GeneTreei ENSGT00550000074525.
    HOGENOMi HOG000067326.
    HOVERGENi HBG000009.
    KOi K11584.
    OMAi ESHCRAD.
    OrthoDBi EOG7C2R2S.
    PhylomeDBi Q60996.
    TreeFami TF105556.

    Enzyme and pathway databases

    Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_203336. Degradation of beta-catenin by the destruction complex.
    REACT_207679. Separation of Sister Chromatids.
    REACT_216784. disassembly of the destruction complex and recruitment of AXIN to the membrane.
    REACT_218396. Beta-catenin phosphorylation cascade.

    Miscellaneous databases

    ChiTaRSi PPP2R5C. mouse.
    NextBioi 304827.
    PROi Q60996.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q60996.
    Genevestigatori Q60996.

    Family and domain databases

    InterProi IPR016024. ARM-type_fold.
    IPR002554. PP2A_B56.
    [Graphical view ]
    PANTHERi PTHR10257. PTHR10257. 1 hit.
    Pfami PF01603. B56. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF028043. PP2A_B56. 1 hit.
    SUPFAMi SSF48371. SSF48371. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary tumor.
    2. "A truncated isoform of the protein phosphatase 2A B56gamma regulatory subunit may promote genetic instability and cause tumor progression."
      Ito A., Koma Y., Watabe K., Nagano T., Endo Y., Nojima H., Kitamura Y.
      Am. J. Pathol. 162:81-91(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-524 (ISOFORMS 2 AND 3).
    3. "Identification of a novel protein phosphatase 2A regulatory subunit highly expressed in muscle."
      Tehrani M.A., Mumby M.C., Kamibayashi C.
      J. Biol. Chem. 271:5164-5170(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-524 (ISOFORM 1).
      Tissue: T-cell.
    4. "Identification by differential display of annexin-VI, a gene differentially expressed during melanoma progression."
      Francia G., Mitchell S.D., Moss S.E., Hanby A.M., Marshall J.F., Hart I.R.
      Cancer Res. 56:3855-3858(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-524 (ISOFORM 1).
      Strain: C57BL/6.
    5. "Expression of the B56delta subunit of protein phosphatase 2A and Mea1 in mouse spermatogenesis. Identification of a new B56gamma subunit (B56gamma4) specifically expressed in testis."
      Ortega-Lazaro J.C., del Mazo J.
      Cytogenet. Genome Res. 103:345-351(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 326-524 (ISOFORM 4), TISSUE SPECIFICITY.
      Strain: CD-1.
      Tissue: Testis.

    Entry informationi

    Entry namei2A5G_MOUSE
    AccessioniPrimary (citable) accession number: Q60996
    Secondary accession number(s): O35708
    , Q6TQF7, Q99KW8, Q99N67, Q99N68
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3