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Q60996

- 2A5G_MOUSE

UniProt

Q60996 - 2A5G_MOUSE

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Protein
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
Gene
Ppp2r5c
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation By similarity.

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein phosphatase type 2A regulator activity Source: InterPro

GO - Biological processi

  1. DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Ensembl
  2. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: Ensembl
  3. negative regulation of cell proliferation Source: Ensembl
  4. proteasome-mediated ubiquitin-dependent protein catabolic process Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_203336. Degradation of beta-catenin by the destruction complex.
REACT_207679. Separation of Sister Chromatids.
REACT_216784. disassembly of the destruction complex and recruitment of AXIN to the membrane.
REACT_218396. Beta-catenin phosphorylation cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
Alternative name(s):
PP2A B subunit isoform B'-alpha-3
PP2A B subunit isoform B'-gamma
PP2A B subunit isoform B56-gamma
PP2A B subunit isoform PR61-gamma
PP2A B subunit isoform R5-gamma
Gene namesi
Name:Ppp2r5c
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1349475. Ppp2r5c.

Subcellular locationi

Nucleus By similarity. Chromosomecentromere By similarity

GO - Cellular componenti

  1. chromosome, centromeric region Source: UniProtKB
  2. nucleus Source: MGI
  3. protein phosphatase type 2A complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
PRO_0000071458Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ60996.
PaxDbiQ60996.
PRIDEiQ60996.

PTM databases

PhosphoSiteiQ60996.

Expressioni

Tissue specificityi

Highest levels in heart, liver and brain. Lower levels in skeletal muscle, spleen, kidney and lung. Isoform 4 is testis-specific.1 Publication

Gene expression databases

BgeeiQ60996.
GenevestigatoriQ60996.

Interactioni

Subunit structurei

PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGOL1 By similarity. Interacts with SGOL1; the interaction is direct. May interact with TP53 By similarity. Interacts with IER3 and/or ERK kinases; regulates ERK dephosphorylation By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
PPP2R1AP301532EBI-1369292,EBI-302388From a different organism.

Protein-protein interaction databases

BioGridi205070. 1 interaction.
DIPiDIP-24181N.
IntActiQ60996. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ60996.
SMRiQ60996. Positions 34-436.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG264925.
GeneTreeiENSGT00550000074525.
HOGENOMiHOG000067326.
HOVERGENiHBG000009.
KOiK11584.
OMAiESHCRAD.
OrthoDBiEOG7C2R2S.
PhylomeDBiQ60996.
TreeFamiTF105556.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR002554. PP2A_B56.
[Graphical view]
PANTHERiPTHR10257. PTHR10257. 1 hit.
PfamiPF01603. B56. 1 hit.
[Graphical view]
PIRSFiPIRSF028043. PP2A_B56. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 2 (identifier: Q60996-1) [UniParc]FASTAAdd to Basket

Also known as: Gamma-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLTCNKAGSG MVVDAASSNG PFQPVALLHI RDVPPADQEK LFIQKLRQCC    50
VLFDFVSDPL SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEAVHMFA 100
VNMFRTLPPS SNPTGAEFDP EEDEPTLEAA WPHLQLVYEF FLRFLESPDF 150
QPNIAKKYID QKFVLQLLEL FDSEDPRERD FLKTTLHRIY GKFLGLRAYI 200
RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK EEHKIFLLKV 250
LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK 300
EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW 350
NNEYIMSLIS DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM 400
NQKLFDDCTQ QFKAEKLKEK LKMKEREEAW VKIENLAKAN PQYAVYSQAS 450
AVSIPVAMET DGPQFEDVQM LKKTVSDEAR QAQKELKKDR PLVRRKSELP 500
QDPHTEKALE AHCRASELLS QDGR 524
Length:524
Mass (Da):60,824
Last modified:July 19, 2005 - v2
Checksum:iA22DFD34A1FFBD01
GO
Isoform 1 (identifier: Q60996-2) [UniParc]FASTAAdd to Basket

Also known as: Gamma-1

The sequence of this isoform differs from the canonical sequence as follows:
     443-452: YAVYSQASAV → VLKKRVTREC
     453-524: Missing.

Note: No experimental confirmation available.

Show »
Length:452
Mass (Da):52,818
Checksum:iDFD00AF40F422088
GO
Isoform 3 (identifier: Q60996-3) [UniParc]FASTAAdd to Basket

Also known as: Gamma-3

The sequence of this isoform differs from the canonical sequence as follows:
     442-480: Missing.

Show »
Length:485
Mass (Da):56,552
Checksum:iE5BE84FF6E3E01C7
GO
Isoform 4 (identifier: Q60996-4) [UniParc]FASTAAdd to Basket

Also known as: Gamma-4

The sequence of this isoform differs from the canonical sequence as follows:
     482-496: AQKELKKDRPLVRRK → LVGRKAMTATQVRKV
     497-524: Missing.

Show »
Length:496
Mass (Da):57,517
Checksum:i8D2004773C96E41E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei442 – 48039Missing in isoform 3.
VSP_014718Add
BLAST
Alternative sequencei443 – 45210YAVYSQASAV → VLKKRVTREC in isoform 1.
VSP_014719
Alternative sequencei453 – 52472Missing in isoform 1.
VSP_014720Add
BLAST
Alternative sequencei482 – 49615AQKEL…LVRRK → LVGRKAMTATQVRKV in isoform 4.
VSP_038641Add
BLAST
Alternative sequencei497 – 52428Missing in isoform 4.
VSP_038642Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 193SSN → APT in BAB32447. 1 Publication
Sequence conflicti17 – 193SSN → APT in BAB32448. 1 Publication
Sequence conflicti32 – 321D → N in AAB70857. 1 Publication
Sequence conflicti72 – 721A → S1 Publication
Sequence conflicti72 – 721A → S1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC003979 mRNA. Translation: AAH03979.1.
AB055635 mRNA. Translation: BAB32447.1.
AB055636 mRNA. Translation: BAB32448.1.
U37353 mRNA. Translation: AAC52435.1.
U59418 mRNA. Translation: AAB70857.1.
AY389498 mRNA. Translation: AAR26474.1.
CCDSiCCDS36557.1. [Q60996-1]
CCDS36558.1. [Q60996-3]
RefSeqiNP_001074926.1. NM_001081457.2. [Q60996-3]
NP_001074927.1. NM_001081458.2. [Q60996-2]
NP_036153.2. NM_012023.3. [Q60996-1]
UniGeneiMm.240396.
Mm.458056.
Mm.490988.

Genome annotation databases

EnsembliENSMUST00000084985; ENSMUSP00000082053; ENSMUSG00000017843. [Q60996-1]
ENSMUST00000109832; ENSMUSP00000105458; ENSMUSG00000017843. [Q60996-3]
GeneIDi26931.
KEGGimmu:26931.
UCSCiuc007pbk.1. mouse. [Q60996-2]
uc007pbl.1. mouse. [Q60996-1]
uc007pbm.1. mouse. [Q60996-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC003979 mRNA. Translation: AAH03979.1 .
AB055635 mRNA. Translation: BAB32447.1 .
AB055636 mRNA. Translation: BAB32448.1 .
U37353 mRNA. Translation: AAC52435.1 .
U59418 mRNA. Translation: AAB70857.1 .
AY389498 mRNA. Translation: AAR26474.1 .
CCDSi CCDS36557.1. [Q60996-1 ]
CCDS36558.1. [Q60996-3 ]
RefSeqi NP_001074926.1. NM_001081457.2. [Q60996-3 ]
NP_001074927.1. NM_001081458.2. [Q60996-2 ]
NP_036153.2. NM_012023.3. [Q60996-1 ]
UniGenei Mm.240396.
Mm.458056.
Mm.490988.

3D structure databases

ProteinModelPortali Q60996.
SMRi Q60996. Positions 34-436.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 205070. 1 interaction.
DIPi DIP-24181N.
IntActi Q60996. 1 interaction.

PTM databases

PhosphoSitei Q60996.

Proteomic databases

MaxQBi Q60996.
PaxDbi Q60996.
PRIDEi Q60996.

Protocols and materials databases

DNASUi 26931.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000084985 ; ENSMUSP00000082053 ; ENSMUSG00000017843 . [Q60996-1 ]
ENSMUST00000109832 ; ENSMUSP00000105458 ; ENSMUSG00000017843 . [Q60996-3 ]
GeneIDi 26931.
KEGGi mmu:26931.
UCSCi uc007pbk.1. mouse. [Q60996-2 ]
uc007pbl.1. mouse. [Q60996-1 ]
uc007pbm.1. mouse. [Q60996-3 ]

Organism-specific databases

CTDi 5527.
MGIi MGI:1349475. Ppp2r5c.

Phylogenomic databases

eggNOGi NOG264925.
GeneTreei ENSGT00550000074525.
HOGENOMi HOG000067326.
HOVERGENi HBG000009.
KOi K11584.
OMAi ESHCRAD.
OrthoDBi EOG7C2R2S.
PhylomeDBi Q60996.
TreeFami TF105556.

Enzyme and pathway databases

Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_203336. Degradation of beta-catenin by the destruction complex.
REACT_207679. Separation of Sister Chromatids.
REACT_216784. disassembly of the destruction complex and recruitment of AXIN to the membrane.
REACT_218396. Beta-catenin phosphorylation cascade.

Miscellaneous databases

ChiTaRSi PPP2R5C. mouse.
NextBioi 304827.
PROi Q60996.
SOURCEi Search...

Gene expression databases

Bgeei Q60996.
Genevestigatori Q60996.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR002554. PP2A_B56.
[Graphical view ]
PANTHERi PTHR10257. PTHR10257. 1 hit.
Pfami PF01603. B56. 1 hit.
[Graphical view ]
PIRSFi PIRSF028043. PP2A_B56. 1 hit.
SUPFAMi SSF48371. SSF48371. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  2. "A truncated isoform of the protein phosphatase 2A B56gamma regulatory subunit may promote genetic instability and cause tumor progression."
    Ito A., Koma Y., Watabe K., Nagano T., Endo Y., Nojima H., Kitamura Y.
    Am. J. Pathol. 162:81-91(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-524 (ISOFORMS 2 AND 3).
  3. "Identification of a novel protein phosphatase 2A regulatory subunit highly expressed in muscle."
    Tehrani M.A., Mumby M.C., Kamibayashi C.
    J. Biol. Chem. 271:5164-5170(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-524 (ISOFORM 1).
    Tissue: T-cell.
  4. "Identification by differential display of annexin-VI, a gene differentially expressed during melanoma progression."
    Francia G., Mitchell S.D., Moss S.E., Hanby A.M., Marshall J.F., Hart I.R.
    Cancer Res. 56:3855-3858(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-524 (ISOFORM 1).
    Strain: C57BL/6.
  5. "Expression of the B56delta subunit of protein phosphatase 2A and Mea1 in mouse spermatogenesis. Identification of a new B56gamma subunit (B56gamma4) specifically expressed in testis."
    Ortega-Lazaro J.C., del Mazo J.
    Cytogenet. Genome Res. 103:345-351(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 326-524 (ISOFORM 4), TISSUE SPECIFICITY.
    Strain: CD-1.
    Tissue: Testis.

Entry informationi

Entry namei2A5G_MOUSE
AccessioniPrimary (citable) accession number: Q60996
Secondary accession number(s): O35708
, Q6TQF7, Q99KW8, Q99N67, Q99N68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 19, 2005
Last modified: September 3, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi