Q60996 (2A5G_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform

Alternative name(s):
PP2A B subunit isoform B'-alpha-3
PP2A B subunit isoform B'-gamma
PP2A B subunit isoform B56-gamma
PP2A B subunit isoform PR61-gamma
PP2A B subunit isoform R5-gamma

Gene names

Name:

Ppp2r5c

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation By similarity.
Subunit structure	PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGOL1 By similarity. Interacts with SGOL1; the interaction is direct. May interact with TP53 By similarity. Interacts with IER3 and/or ERK kinases; regulates ERK dephosphorylation By similarity.
Subcellular location	Nucleus By similarity. Chromosome › centromere By similarity.
Tissue specificity	Highest levels in heart, liver and brain. Lower levels in skeletal muscle, spleen, kidney and lung. Isoform 4 is testis-specific. Ref.5
Sequence similarities	Belongs to the phosphatase 2A regulatory subunit B56 family.

Ontologies

Keywords
Cellular component	Centromere Chromosome Nucleus
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence orthology PubMed 16717086. Source: MGI signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome, centromeric region Inferred from sequence or structural similarity. Source: UniProtKB nucleus Traceable author statement PubMed 10781942. Source: MGI protein phosphatase type 2A complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	protein phosphatase type 2A regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PPP2R1A	P30153	2	EBI-1369292,EBI-302388	From a different organism.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: Q60996-1)

Also known as: Gamma-2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q60996-2)

Also known as: Gamma-1;

The sequence of this isoform differs from the canonical sequence as follows:
     443-452: YAVYSQASAV → VLKKRVTREC
     453-524: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q60996-3)

Also known as: Gamma-3;

The sequence of this isoform differs from the canonical sequence as follows:
     442-480: Missing.

Isoform 4 (identifier: Q60996-4)

Also known as: Gamma-4;

The sequence of this isoform differs from the canonical sequence as follows:
     482-496: AQKELKKDRPLVRRK → LVGRKAMTATQVRKV
     497-524: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform		PRO_0000071458
Amino acid modifications
	Modified residue	497	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	442 – 480	39	Missing in isoform 3.		VSP_014718
	Alternative sequence	443 – 452	10	YAVYSQASAV → VLKKRVTREC in isoform 1.		VSP_014719
	Alternative sequence	453 – 524	72	Missing in isoform 1.		VSP_014720
	Alternative sequence	482 – 496	15	AQKEL…LVRRK → LVGRKAMTATQVRKV in isoform 4.		VSP_038641
	Alternative sequence	497 – 524	28	Missing in isoform 4.		VSP_038642
Experimental info
	Sequence conflict	17 – 19	3	SSN → APT in BAB32447. Ref.2
	Sequence conflict	17 – 19	3	SSN → APT in BAB32448. Ref.2
	Sequence conflict	32	1	D → N in AAB70857. Ref.4
	Sequence conflict	72	1	A → S Ref.3
	Sequence conflict	72	1	A → S Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 (Gamma-2) [UniParc]. Last modified July 19, 2005. Version 2. Checksum: A22DFD34A1FFBD01	FASTA	524	60,824
	10 20 30 40 50 60 MLTCNKAGSG MVVDAASSNG PFQPVALLHI RDVPPADQEK LFIQKLRQCC VLFDFVSDPL 70 80 90 100 110 120 SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEAVHMFA VNMFRTLPPS SNPTGAEFDP 130 140 150 160 170 180 EEDEPTLEAA WPHLQLVYEF FLRFLESPDF QPNIAKKYID QKFVLQLLEL FDSEDPRERD 190 200 210 220 230 240 FLKTTLHRIY GKFLGLRAYI RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK 250 260 270 280 290 300 EEHKIFLLKV LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK 310 320 330 340 350 360 EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW NNEYIMSLIS 370 380 390 400 410 420 DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM NQKLFDDCTQ QFKAEKLKEK 430 440 450 460 470 480 LKMKEREEAW VKIENLAKAN PQYAVYSQAS AVSIPVAMET DGPQFEDVQM LKKTVSDEAR 490 500 510 520 QAQKELKKDR PLVRRKSELP QDPHTEKALE AHCRASELLS QDGR
	Isoform 1 (Gamma-1) [UniParc]. Checksum: DFD00AF40F422088	FASTA	452	52,818
	10 20 30 40 50 60 MLTCNKAGSG MVVDAASSNG PFQPVALLHI RDVPPADQEK LFIQKLRQCC VLFDFVSDPL 70 80 90 100 110 120 SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEAVHMFA VNMFRTLPPS SNPTGAEFDP 130 140 150 160 170 180 EEDEPTLEAA WPHLQLVYEF FLRFLESPDF QPNIAKKYID QKFVLQLLEL FDSEDPRERD 190 200 210 220 230 240 FLKTTLHRIY GKFLGLRAYI RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK 250 260 270 280 290 300 EEHKIFLLKV LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK 310 320 330 340 350 360 EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW NNEYIMSLIS 370 380 390 400 410 420 DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM NQKLFDDCTQ QFKAEKLKEK 430 440 450 LKMKEREEAW VKIENLAKAN PQVLKKRVTR EC
	Isoform 3 (Gamma-3) [UniParc]. Checksum: E5BE84FF6E3E01C7	FASTA	485	56,552
	10 20 30 40 50 60 MLTCNKAGSG MVVDAASSNG PFQPVALLHI RDVPPADQEK LFIQKLRQCC VLFDFVSDPL 70 80 90 100 110 120 SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEAVHMFA VNMFRTLPPS SNPTGAEFDP 130 140 150 160 170 180 EEDEPTLEAA WPHLQLVYEF FLRFLESPDF QPNIAKKYID QKFVLQLLEL FDSEDPRERD 190 200 210 220 230 240 FLKTTLHRIY GKFLGLRAYI RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK 250 260 270 280 290 300 EEHKIFLLKV LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK 310 320 330 340 350 360 EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW NNEYIMSLIS 370 380 390 400 410 420 DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM NQKLFDDCTQ QFKAEKLKEK 430 440 450 460 470 480 LKMKEREEAW VKIENLAKAN PQAQKELKKD RPLVRRKSEL PQDPHTEKAL EAHCRASELL SQDGR
	Isoform 4 (Gamma-4) [UniParc]. Checksum: 8D2004773C96E41E	FASTA	496	57,517
	10 20 30 40 50 60 MLTCNKAGSG MVVDAASSNG PFQPVALLHI RDVPPADQEK LFIQKLRQCC VLFDFVSDPL 70 80 90 100 110 120 SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEAVHMFA VNMFRTLPPS SNPTGAEFDP 130 140 150 160 170 180 EEDEPTLEAA WPHLQLVYEF FLRFLESPDF QPNIAKKYID QKFVLQLLEL FDSEDPRERD 190 200 210 220 230 240 FLKTTLHRIY GKFLGLRAYI RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK 250 260 270 280 290 300 EEHKIFLLKV LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK 310 320 330 340 350 360 EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW NNEYIMSLIS 370 380 390 400 410 420 DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM NQKLFDDCTQ QFKAEKLKEK 430 440 450 460 470 480 LKMKEREEAW VKIENLAKAN PQYAVYSQAS AVSIPVAMET DGPQFEDVQM LKKTVSDEAR 490 QLVGRKAMTA TQVRKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary tumor.
[2]	"A truncated isoform of the protein phosphatase 2A B56gamma regulatory subunit may promote genetic instability and cause tumor progression." Ito A., Koma Y., Watabe K., Nagano T., Endo Y., Nojima H., Kitamura Y. Am. J. Pathol. 162:81-91(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-524 (ISOFORMS 2 AND 3).
[3]	"Identification of a novel protein phosphatase 2A regulatory subunit highly expressed in muscle." Tehrani M.A., Mumby M.C., Kamibayashi C. J. Biol. Chem. 271:5164-5170(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-524 (ISOFORM 1). Tissue: T-cell.
[4]	"Identification by differential display of annexin-VI, a gene differentially expressed during melanoma progression." Francia G., Mitchell S.D., Moss S.E., Hanby A.M., Marshall J.F., Hart I.R. Cancer Res. 56:3855-3858(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-524 (ISOFORM 1). Strain: C57BL/6.
[5]	"Expression of the B56delta subunit of protein phosphatase 2A and Mea1 in mouse spermatogenesis. Identification of a new B56gamma subunit (B56gamma4) specifically expressed in testis." Ortega-Lazaro J.C., del Mazo J. Cytogenet. Genome Res. 103:345-351(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 326-524 (ISOFORM 4), TISSUE SPECIFICITY. Strain: CD-1. Tissue: Testis.
[6]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC003979 mRNA. Translation: AAH03979.1. AB055635 mRNA. Translation: BAB32447.1. AB055636 mRNA. Translation: BAB32448.1. U37353 mRNA. Translation: AAC52435.1. U59418 mRNA. Translation: AAB70857.1. AY389498 mRNA. Translation: AAR26474.1.
IPI	IPI00118131. IPI00408024. IPI00457543. IPI00466617.
RefSeq	NP_001074926.1. NM_001081457.1. NP_001074927.1. NM_001081458.1. NP_036153.2. NM_012023.2.
UniGene	Mm.240396. Mm.458056. Mm.490988.
3D structure databases
ProteinModelPortal	Q60996.
SMR	Q60996. Positions 34-436.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24181N.
IntAct	Q60996. 1 interaction.
PTM databases
PhosphoSite	Q60996.
Proteomic databases
PaxDb	Q60996.
PRIDE	Q60996.
Protocols and materials databases
DNASU	26931.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000084985; ENSMUSP00000082053; ENSMUSG00000017843. ENSMUST00000109832; ENSMUSP00000105458; ENSMUSG00000017843.
GeneID	26931.
KEGG	mmu:26931.
UCSC	uc007pbk.1. mouse. uc007pbl.1. mouse. uc007pbm.1. mouse.
Organism-specific databases
CTD	5527.
MGI	MGI:1349475. Ppp2r5c.
Phylogenomic databases
eggNOG	NOG264925.
GeneTree	ENSGT00550000074525.
HOGENOM	HOG000067326.
HOVERGEN	HBG000009.
KO	K11584.
OMA	ESHCRAD.
Gene expression databases
Bgee	Q60996.
Genevestigator	Q60996.
GermOnline	ENSMUSG00000017843. Mus musculus.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR002554. PP2A_B56. [Graphical view]
PANTHER	PTHR10257. PTHR10257. 1 hit.
Pfam	PF01603. B56. 1 hit. [Graphical view]
PIRSF	PIRSF028043. PP2A_B56. 1 hit.
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	PPP2R5C. mouse.
NextBio	304827.
SOURCE	Search...

Entry information

Entry name

2A5G_MOUSE

Accession

Primary (citable) accession number: Q60996
Secondary accession number(s): O35708 , Q6TQF7, Q99KW8, Q99N67, Q99N68

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 19, 2005
Last modified:	April 3, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program