Reviewed, UniProtKB/Swiss-Prot Q60992 (VAV2_MOUSE)

Names and origin

Protein names

Recommended name:
    Guanine nucleotide exchange factor VAV2
      Short name=VAV-2

Gene names

Name:

Vav2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	868 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Ref.3
Subunit structure	Interacts (via SH2 domains) with the phosphorylated form of EPHA2. Ref.3
Sequence similarities	Contains 1 CH (calponin-homology) domain. Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 SH2 domain. Contains 2 SH3 domains.

Ontologies

Keywords
Biological process	Angiogenesis
Domain	Repeat SH2 domain SH3 domain Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	angiogenesis Inferred from electronic annotation. Source: UniProtKB-KW cell migration Inferred from genetic interaction. Source: MGI lamellipodium assembly Inferred from genetic interaction. Source: MGI positive regulation of phosphoinositide 3-kinase activity Inferred from genetic interaction. Source: MGI regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro small GTPase mediated signal transduction Inferred from mutant phenotype. Source: MGI
Cellular component	cytoplasm Inferred from direct assay. Source: MGI plasma membrane Inferred from direct assay. Source: MGI
Molecular function	Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro epidermal growth factor receptor binding Inferred from physical interaction. Source: MGI zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 868	868	Guanine nucleotide exchange factor VAV2		PRO_0000080985
Regions
	Domain	1 – 119	119	CH
	Domain	193 – 371	179	DH
	Domain	400 – 502	103	PH
	Domain	576 – 642	67	SH3 1
	Domain	663 – 757	95	SH2
	Domain	806 – 867	62	SH3 2
	Zinc finger	513 – 562	50	Phorbol-ester/DAG-type
Amino acid modifications
	Modified residue	142	1	Phosphotyrosine; by EGFR By similarity
	Modified residue	159	1	Phosphotyrosine; by EGFR By similarity
	Modified residue	172	1	Phosphotyrosine; by EGFR By similarity
	Modified residue	566	1	Phosphoserine By similarity
	Modified residue	616	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60992-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: D18581E7EEB2DBC2	FASTA	868	99,915
	10 20 30 40 50 60 MEQWRQCGRW LIDCKVLPPN HRVVWPSAVV FDLAQALRDG VLLCQLLHNL SPGSIDLKDI 70 80 90 100 110 120 NFRPQMSQFL CLKNIRTFLK VCHDKFGLRN SELFDPFDLF DVRDFGKVIS AVSRLSLHSI 130 140 150 160 170 180 AQSKGIRPFP SEETAENDDD VYRSLEELAD EHDLGEDIYD CVPCEDEGDD IYEDIIKVEV 190 200 210 220 230 240 QQPMKMGMTE DDKRSCCLLE IQETEAKYYR TLEDIEKNYM GPLRLVLSPA DMAAVFINLE 250 260 270 280 290 300 DLIKVHHSFL RAIDVSMMAG GSTLAKVFLE FKERLLIYGE YCSHMEHAQS TLNQLLASRE 310 320 330 340 350 360 DFRQKVEECT LRVQDGKFKL QDLLVVPMQR VLKYHLLLKE LLSHSADRPE RQQLKEALEA 370 380 390 400 410 420 MQDLAMYINE VKRDKETLKK ISEFQCSIEN LQVKLEEFGR PKIDGELKVR SIVNHTKQDR 430 440 450 460 470 480 YLFLFDKVVI VCKRKGYSYE LKEVIELLFH KMTDDPMHNK DIKKWSYGFY LIHLQGKQGF 490 500 510 520 530 540 QFFCKTEDMK RKWMEQFEMA MSNIKPDKAN ANHHSFQMYT FDKTTNCKAC KMFLRGTFYQ 550 560 570 580 590 600 GYLCTRCGVG AHKECLEVIP PCKMSSPADV DAPGAGPGPK MVAVQNYHGN PAPPGKPVLT 610 620 630 640 650 660 FQTGDVIELL RGDPDSPWWE GRLVQTRKSG YFPSSSVKPC PVDGRPPTGR PPSREIDYTA 670 680 690 700 710 720 YPWFAGNMER QQTDNLLKSH ASGTYLIRER PAEAERFAIS IKFNDEVKHI KVVEKDSWIH 730 740 750 760 770 780 ITEAKKFESL LELVEYYQCH SLKESFKQLD TTLKFPYKSR ERTTSRASSR SPASCASYNF 790 800 810 820 830 840 SFLSPQGLSF APQAPSAPFW SVFTPRVIGT AVARYNFAAR DMRELSLREG DVVKIYSRIG 850 860 GDQGWWKGET NGRIGWFPST YVEEEGVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of murine vav2, a member of the vav family of proto-oncogenes." Schuebel K.E., Bustelo X.R., Nielsen D.A., Song B.J., Barbacid M., Goldman D., Lee I.J. Oncogene 13:363-371(1996) [PubMed: 8710375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Essential role of Vav family guanine nucleotide exchange factors in EphA receptor-mediated angiogenesis." Hunter S.G., Zhuang G., Brantley-Sieders D.M., Swat W., Cowan C.W., Chen J. Mol. Cell. Biol. 26:4830-4842(2006) [PubMed: 16782872] [Abstract] Cited for: FUNCTION, INTERACTION WITH EPHA2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U37017 mRNA. Translation: AAC52761.1. AL731552, AL772282 Genomic DNA. Translation: CAM13732.1. AL772282, AL731552 Genomic DNA. Translation: CAM21000.1.
IPI	IPI00338599.
RefSeq	NP_033526.1.
UniGene	Mm.179011
3D structure databases
SMR	Q60992. Positions 1-131, 189-519, 510-563, 580-814, 810-868.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q60992. 3 interactions.
STRING	Q60992.
PTM databases
PhosphoSite	Q60992.
Proteomic databases
PRIDE	Q60992.
Genome annotation databases
Ensembl	ENSMUST00000056176; ENSMUSP00000062782; ENSMUSG00000009621; Mus musculus. [Genome view]
GeneID	22325.
KEGG	mmu:22325.
UCSC	uc008ixh.1. mouse.
Organism-specific databases
CTD	22325.
MGI	MGI:102718. Vav2.
Phylogenomic databases
eggNOG	roNOG13080.
HOGENOM	HBG445260.
HOVERGEN	Q60992.
InParanoid	Q60992.
OMA	YPWFAGN.
Gene expression databases
ArrayExpress	Q60992.
Bgee	Q60992.
CleanEx	MM_VAV2.
Genevestigator	Q60992.
GermOnline	ENSMUSG00000009621. Mus musculus.
Family and domain databases
InterPro	IPR016146. Calponin-homology. IPR001715. Calponin_act_bd. IPR000219. DH-domain. IPR001331. GDS_CDC24_CS. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR000980. SH2. IPR011511. SH3_2. IPR001452. SH3_domain. IPR020473. SH3_region. [Graphical view]
Gene3D	G3DSA:1.10.418.10. Calponin-homology. 1 hit. G3DSA:1.20.900.10. RhoGEF. 1 hit. G3DSA:3.30.505.10. SH2. 1 hit.
Pfam	PF00130. C1_1. 1 hit. PF00169. PH. 1 hit. PF00621. RhoGEF. 1 hit. PF00017. SH2. 1 hit. PF07653. SH3_2. 2 hits. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN.
SMART	SM00109. C1. 1 hit. SM00033. CH. 1 hit. SM00233. PH. 1 hit. SM00325. RhoGEF. 1 hit. SM00252. SH2. 1 hit. SM00326. SH3. 2 hits. [Graphical view]
PROSITE	PS50021. CH. 1 hit. PS00741. DH_1. 1 hit. PS50010. DH_2. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 2 hits. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	302553.
SOURCE	Search...

Entry information

Entry name

VAV2_MOUSE

Accession

Primary (citable) accession number: Q60992
Secondary accession number(s): A2AH49

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	February 9, 2010
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)