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Q60991

- CP7B1_MOUSE

UniProt

Q60991 - CP7B1_MOUSE

Protein

25-hydroxycholesterol 7-alpha-hydroxylase

Gene

Cyp7b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Cholest-5-ene-3-beta,25-diol + NADPH + O2 = cholest-5-ene-3-beta,7-alpha,25-triol + NADP+ + H2O.
    Cholest-5-ene-3-beta,27-diol + NADPH + O2 = cholest-5-ene-3-beta,7-alpha,27-triol + NADP+ + H2O.

    Cofactori

    Heme group.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi447 – 4471Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. 25-hydroxycholesterol 7alpha-hydroxylase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. oxysterol 7-alpha-hydroxylase activity Source: UniProtKB

    GO - Biological processi

    1. bile acid biosynthetic process Source: UniProtKB
    2. cholesterol metabolic process Source: UniProtKB-KW
    3. digestion Source: UniProtKB
    4. negative regulation of intracellular estrogen receptor signaling pathway Source: MGI
    5. positive regulation of epithelial cell proliferation Source: MGI
    6. prostate gland epithelium morphogenesis Source: MGI

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BRENDAi1.14.13.100. 3474.
    ReactomeiREACT_207530. Synthesis of bile acids and bile salts.
    REACT_226440. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
    REACT_227038. Endogenous sterols.
    UniPathwayiUPA00221.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    25-hydroxycholesterol 7-alpha-hydroxylase (EC:1.14.13.100)
    Alternative name(s):
    Cytochrome P450 7B1
    Hippocampal transcript 1 protein
    Short name:
    HCT-1
    Oxysterol 7-alpha-hydroxylase
    Gene namesi
    Name:Cyp7b1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:104978. Cyp7b1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. intracellular membrane-bounded organelle Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 50750725-hydroxycholesterol 7-alpha-hydroxylasePRO_0000051907Add
    BLAST

    Proteomic databases

    PaxDbiQ60991.
    PRIDEiQ60991.

    PTM databases

    PhosphoSiteiQ60991.

    Expressioni

    Tissue specificityi

    Highly expressed in brain; also expressed in liver and kidney.

    Gene expression databases

    BgeeiQ60991.
    GenevestigatoriQ60991.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-1864111.
    STRINGi10090.ENSMUSP00000037487.

    Structurei

    3D structure databases

    ProteinModelPortaliQ60991.
    SMRiQ60991. Positions 37-503.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00550000074551.
    HOGENOMiHOG000231026.
    HOVERGENiHBG051100.
    InParanoidiQ9CZ39.
    KOiK07430.
    OMAiQYQLVIK.
    OrthoDBiEOG7J9VP6.
    TreeFamiTF105090.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR024204. Cyt_P450_CYP7A1-type.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
    PRINTSiPR00465. EP450IV.
    SUPFAMiSSF48264. SSF48264. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q60991-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQGATTLDAA SPGPLALLGL LFAATLLLSA LFLLTRRTRR PREPPLIKGW    50
    LPYLGMALKF FKDPLTFLKT LQRQHGDTFT VFLVGKYITF VLNPFQYQYV 100
    TKNPKQLSFQ KFSSRLSAKA FSVKKLLTDD DLNEDVHRAY LLLQGKPLDA 150
    LLETMIQEVK ELFESQLLKI TDWNTERIFA FCGSLVFEIT FATLYGKILA 200
    GNKKQIISEL RDDFFKFDDM FPYLVSDIPI QLLRNEESMQ KKIIKCLTSE 250
    KVAQMQGQSK IVQERQDLLK RYYRHDDPEI GAHHLGFLWA SLANTIPAMF 300
    WAMYYILRHP EAMEALRDEI DSFLQSTGQK KGPGISVHFT REQLDSLVCL 350
    ESTILEVLRL CSYSSIIREV QEDMNLSLES KSFSLRKGDF VALFPPLIHN 400
    DPEIFDAPKE FRFDRFIEDG KKKSTFFKGG KKLKTYVMPF GLGTSKCPGR 450
    YFAVNEMKLL LIMLLTYFDL EIIDRKPIGL NHSRMFLGIQ HPDSAVSFRY 500
    KAKSWRS 507
    Length:507
    Mass (Da):58,470
    Last modified:July 27, 2011 - v2
    Checksum:i628A0D3270C04DA4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti265 – 2651R → S in AAA92615. (PubMed:8530364)Curated
    Sequence conflicti278 – 2781P → S in AAA92615. (PubMed:8530364)Curated
    Sequence conflicti432 – 4321K → R in AAA92615. (PubMed:8530364)Curated
    Sequence conflicti463 – 4631M → E in AAA92615. (PubMed:8530364)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36993 mRNA. Translation: AAA92615.1.
    AK013034 mRNA. Translation: BAB28613.1.
    CCDSiCCDS17254.1.
    RefSeqiNP_031851.3. NM_007825.4.
    XP_006535446.1. XM_006535383.1.
    UniGeneiMm.316000.

    Genome annotation databases

    EnsembliENSMUST00000035625; ENSMUSP00000037487; ENSMUSG00000039519.
    GeneIDi13123.
    KEGGimmu:13123.
    UCSCiuc008orm.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36993 mRNA. Translation: AAA92615.1 .
    AK013034 mRNA. Translation: BAB28613.1 .
    CCDSi CCDS17254.1.
    RefSeqi NP_031851.3. NM_007825.4.
    XP_006535446.1. XM_006535383.1.
    UniGenei Mm.316000.

    3D structure databases

    ProteinModelPortali Q60991.
    SMRi Q60991. Positions 37-503.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1864111.
    STRINGi 10090.ENSMUSP00000037487.

    PTM databases

    PhosphoSitei Q60991.

    Proteomic databases

    PaxDbi Q60991.
    PRIDEi Q60991.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035625 ; ENSMUSP00000037487 ; ENSMUSG00000039519 .
    GeneIDi 13123.
    KEGGi mmu:13123.
    UCSCi uc008orm.2. mouse.

    Organism-specific databases

    CTDi 9420.
    MGIi MGI:104978. Cyp7b1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00550000074551.
    HOGENOMi HOG000231026.
    HOVERGENi HBG051100.
    InParanoidi Q9CZ39.
    KOi K07430.
    OMAi QYQLVIK.
    OrthoDBi EOG7J9VP6.
    TreeFami TF105090.

    Enzyme and pathway databases

    UniPathwayi UPA00221 .
    BRENDAi 1.14.13.100. 3474.
    Reactomei REACT_207530. Synthesis of bile acids and bile salts.
    REACT_226440. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
    REACT_227038. Endogenous sterols.

    Miscellaneous databases

    NextBioi 283166.
    PROi Q60991.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q60991.
    Genevestigatori Q60991.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR024204. Cyt_P450_CYP7A1-type.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
    PRINTSi PR00465. EP450IV.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain and Liver.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.

    Entry informationi

    Entry nameiCP7B1_MOUSE
    AccessioniPrimary (citable) accession number: Q60991
    Secondary accession number(s): Q9CZ39
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3