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Q60991 (CP7B1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
25-hydroxycholesterol 7-alpha-hydroxylase
EC=1.14.13.100
Alternative name(s):
Cytochrome P450 7B1
Hippocampal transcript 1 protein
Short name=HCT-1
Oxysterol 7-alpha-hydroxylase
Gene names
Name:Cyp7b1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length507 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Cholest-5-ene-3-beta,25-diol + NADPH + O2 = cholest-5-ene-3-beta,7-alpha,25-triol + NADP+ + H2O.

Cholest-5-ene-3-beta,27-diol + NADPH + O2 = cholest-5-ene-3-beta,7-alpha,27-triol + NADP+ + H2O.

Cofactor

Heme group By similarity.

Pathway

Lipid metabolism; bile acid biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Highly expressed in brain; also expressed in liver and kidney.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Biological processCholesterol metabolism
Lipid metabolism
Steroid metabolism
Sterol metabolism
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   LigandHeme
Iron
Metal-binding
NADP
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbile acid biosynthetic process

Inferred from direct assay PubMed 10748047. Source: UniProtKB

cholesterol metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

digestion

Traceable author statement PubMed 10748047. Source: UniProtKB

negative regulation of intracellular estrogen receptor signaling pathway

Inferred from mutant phenotype PubMed 12370428. Source: MGI

positive regulation of epithelial cell proliferation

Inferred from mutant phenotype PubMed 12370428. Source: MGI

prostate gland epithelium morphogenesis

Inferred from mutant phenotype PubMed 12370428. Source: MGI

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

intracellular membrane-bounded organelle

Traceable author statement PubMed 10748047. Source: UniProtKB

   Molecular_function25-hydroxycholesterol 7alpha-hydroxylase activity

Inferred from electronic annotation. Source: EC

electron carrier activity

Inferred from electronic annotation. Source: InterPro

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

oxysterol 7-alpha-hydroxylase activity

Inferred from direct assay PubMed 10748047. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 50750725-hydroxycholesterol 7-alpha-hydroxylase
PRO_0000051907

Sites

Metal binding4471Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict2651R → S in AAA92615. Ref.1
Sequence conflict2781P → S in AAA92615. Ref.1
Sequence conflict4321K → R in AAA92615. Ref.1
Sequence conflict4631M → E in AAA92615. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q60991 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 628A0D3270C04DA4

FASTA50758,470
        10         20         30         40         50         60 
MQGATTLDAA SPGPLALLGL LFAATLLLSA LFLLTRRTRR PREPPLIKGW LPYLGMALKF 

        70         80         90        100        110        120 
FKDPLTFLKT LQRQHGDTFT VFLVGKYITF VLNPFQYQYV TKNPKQLSFQ KFSSRLSAKA 

       130        140        150        160        170        180 
FSVKKLLTDD DLNEDVHRAY LLLQGKPLDA LLETMIQEVK ELFESQLLKI TDWNTERIFA 

       190        200        210        220        230        240 
FCGSLVFEIT FATLYGKILA GNKKQIISEL RDDFFKFDDM FPYLVSDIPI QLLRNEESMQ 

       250        260        270        280        290        300 
KKIIKCLTSE KVAQMQGQSK IVQERQDLLK RYYRHDDPEI GAHHLGFLWA SLANTIPAMF 

       310        320        330        340        350        360 
WAMYYILRHP EAMEALRDEI DSFLQSTGQK KGPGISVHFT REQLDSLVCL ESTILEVLRL 

       370        380        390        400        410        420 
CSYSSIIREV QEDMNLSLES KSFSLRKGDF VALFPPLIHN DPEIFDAPKE FRFDRFIEDG 

       430        440        450        460        470        480 
KKKSTFFKGG KKLKTYVMPF GLGTSKCPGR YFAVNEMKLL LIMLLTYFDL EIIDRKPIGL 

       490        500 
NHSRMFLGIQ HPDSAVSFRY KAKSWRS

« Hide

References

« Hide 'large scale' references
[1]"A novel cytochrome P450 expressed primarily in brain."
Stapleton G., Steel M., Richardson M., Mason J.O., Rose K.A., Morris R.G., Lathe R.
J. Biol. Chem. 270:29739-29745(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain and Liver.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U36993 mRNA. Translation: AAA92615.1.
AK013034 mRNA. Translation: BAB28613.1.
IPIIPI00122818.
RefSeqNP_031851.3. NM_007825.4.
UniGeneMm.316000.

3D structure databases

ProteinModelPortalQ60991.
SMRQ60991. Positions 37-503.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-1864111.
STRING10090.ENSMUSP00000037487.

PTM databases

PhosphoSiteQ60991.

Proteomic databases

PaxDbQ60991.
PRIDEQ60991.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000035625; ENSMUSP00000037487; ENSMUSG00000039519.
GeneID13123.
KEGGmmu:13123.
UCSCuc008orm.2. mouse.

Organism-specific databases

CTD9420.
MGIMGI:104978. Cyp7b1.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00550000074551.
HOGENOMHOG000231026.
HOVERGENHBG051100.
InParanoidQ9CZ39.
KOK07430.
OMAMFWAMYY.
OrthoDBEOG476K08.

Enzyme and pathway databases

BRENDA1.14.13.100. 3474.
UniPathwayUPA00221.

Gene expression databases

BgeeQ60991.
GenevestigatorQ60991.
GermOnlineENSMUSG00000039519. Mus musculus.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PIRSFPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSPR00465. EP450IV.
SUPFAMSSF48264. Cytochrome_P450. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio283166.
SOURCESearch...

Entry information

Entry nameCP7B1_MOUSE
AccessionPrimary (citable) accession number: Q60991
Secondary accession number(s): Q9CZ39
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: May 29, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents