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Q60991

- CP7B1_MOUSE

UniProt

Q60991 - CP7B1_MOUSE

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Protein

25-hydroxycholesterol 7-alpha-hydroxylase

Gene

Cyp7b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Cholest-5-ene-3-beta,25-diol + NADPH + O2 = cholest-5-ene-3-beta,7-alpha,25-triol + NADP+ + H2O.
Cholest-5-ene-3-beta,27-diol + NADPH + O2 = cholest-5-ene-3-beta,7-alpha,27-triol + NADP+ + H2O.

Cofactori

Heme group.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi447 – 4471Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. 25-hydroxycholesterol 7alpha-hydroxylase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. oxysterol 7-alpha-hydroxylase activity Source: UniProtKB

GO - Biological processi

  1. bile acid biosynthetic process Source: UniProtKB
  2. cholesterol metabolic process Source: UniProtKB-KW
  3. digestion Source: UniProtKB
  4. negative regulation of intracellular estrogen receptor signaling pathway Source: MGI
  5. positive regulation of epithelial cell proliferation Source: MGI
  6. prostate gland epithelium morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.100. 3474.
ReactomeiREACT_207530. Synthesis of bile acids and bile salts.
REACT_226440. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
REACT_227038. Endogenous sterols.
UniPathwayiUPA00221.

Names & Taxonomyi

Protein namesi
Recommended name:
25-hydroxycholesterol 7-alpha-hydroxylase (EC:1.14.13.100)
Alternative name(s):
Cytochrome P450 7B1
Hippocampal transcript 1 protein
Short name:
HCT-1
Oxysterol 7-alpha-hydroxylase
Gene namesi
Name:Cyp7b1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:104978. Cyp7b1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. intracellular membrane-bounded organelle Source: UniProtKB
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 50750725-hydroxycholesterol 7-alpha-hydroxylasePRO_0000051907Add
BLAST

Proteomic databases

MaxQBiQ60991.
PaxDbiQ60991.
PRIDEiQ60991.

PTM databases

PhosphoSiteiQ60991.

Expressioni

Tissue specificityi

Highly expressed in brain; also expressed in liver and kidney.

Gene expression databases

BgeeiQ60991.
GenevestigatoriQ60991.

Interactioni

Protein-protein interaction databases

MINTiMINT-1864111.
STRINGi10090.ENSMUSP00000037487.

Structurei

3D structure databases

ProteinModelPortaliQ60991.
SMRiQ60991. Positions 37-503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00550000074551.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiQ60991.
KOiK07430.
OMAiQYQLVIK.
OrthoDBiEOG7J9VP6.
TreeFamiTF105090.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

Q60991-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQGATTLDAA SPGPLALLGL LFAATLLLSA LFLLTRRTRR PREPPLIKGW
60 70 80 90 100
LPYLGMALKF FKDPLTFLKT LQRQHGDTFT VFLVGKYITF VLNPFQYQYV
110 120 130 140 150
TKNPKQLSFQ KFSSRLSAKA FSVKKLLTDD DLNEDVHRAY LLLQGKPLDA
160 170 180 190 200
LLETMIQEVK ELFESQLLKI TDWNTERIFA FCGSLVFEIT FATLYGKILA
210 220 230 240 250
GNKKQIISEL RDDFFKFDDM FPYLVSDIPI QLLRNEESMQ KKIIKCLTSE
260 270 280 290 300
KVAQMQGQSK IVQERQDLLK RYYRHDDPEI GAHHLGFLWA SLANTIPAMF
310 320 330 340 350
WAMYYILRHP EAMEALRDEI DSFLQSTGQK KGPGISVHFT REQLDSLVCL
360 370 380 390 400
ESTILEVLRL CSYSSIIREV QEDMNLSLES KSFSLRKGDF VALFPPLIHN
410 420 430 440 450
DPEIFDAPKE FRFDRFIEDG KKKSTFFKGG KKLKTYVMPF GLGTSKCPGR
460 470 480 490 500
YFAVNEMKLL LIMLLTYFDL EIIDRKPIGL NHSRMFLGIQ HPDSAVSFRY

KAKSWRS
Length:507
Mass (Da):58,470
Last modified:July 27, 2011 - v2
Checksum:i628A0D3270C04DA4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti265 – 2651R → S in AAA92615. (PubMed:8530364)Curated
Sequence conflicti278 – 2781P → S in AAA92615. (PubMed:8530364)Curated
Sequence conflicti432 – 4321K → R in AAA92615. (PubMed:8530364)Curated
Sequence conflicti463 – 4631M → E in AAA92615. (PubMed:8530364)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U36993 mRNA. Translation: AAA92615.1.
AK013034 mRNA. Translation: BAB28613.1.
CCDSiCCDS17254.1.
RefSeqiNP_031851.3. NM_007825.4.
XP_006535446.1. XM_006535383.1.
UniGeneiMm.316000.

Genome annotation databases

EnsembliENSMUST00000035625; ENSMUSP00000037487; ENSMUSG00000039519.
GeneIDi13123.
KEGGimmu:13123.
UCSCiuc008orm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U36993 mRNA. Translation: AAA92615.1 .
AK013034 mRNA. Translation: BAB28613.1 .
CCDSi CCDS17254.1.
RefSeqi NP_031851.3. NM_007825.4.
XP_006535446.1. XM_006535383.1.
UniGenei Mm.316000.

3D structure databases

ProteinModelPortali Q60991.
SMRi Q60991. Positions 37-503.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1864111.
STRINGi 10090.ENSMUSP00000037487.

PTM databases

PhosphoSitei Q60991.

Proteomic databases

MaxQBi Q60991.
PaxDbi Q60991.
PRIDEi Q60991.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000035625 ; ENSMUSP00000037487 ; ENSMUSG00000039519 .
GeneIDi 13123.
KEGGi mmu:13123.
UCSCi uc008orm.2. mouse.

Organism-specific databases

CTDi 9420.
MGIi MGI:104978. Cyp7b1.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00550000074551.
HOGENOMi HOG000231026.
HOVERGENi HBG051100.
InParanoidi Q60991.
KOi K07430.
OMAi QYQLVIK.
OrthoDBi EOG7J9VP6.
TreeFami TF105090.

Enzyme and pathway databases

UniPathwayi UPA00221 .
BRENDAi 1.14.13.100. 3474.
Reactomei REACT_207530. Synthesis of bile acids and bile salts.
REACT_226440. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
REACT_227038. Endogenous sterols.

Miscellaneous databases

NextBioi 283166.
PROi Q60991.
SOURCEi Search...

Gene expression databases

Bgeei Q60991.
Genevestigatori Q60991.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PIRSFi PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSi PR00465. EP450IV.
SUPFAMi SSF48264. SSF48264. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain and Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.

Entry informationi

Entry nameiCP7B1_MOUSE
AccessioniPrimary (citable) accession number: Q60991
Secondary accession number(s): Q9CZ39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3