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Q60988

- STIL_MOUSE

UniProt

Q60988 - STIL_MOUSE

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Protein

SCL-interrupting locus protein homolog

Gene
Stil, Sil
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1.3 Publications

GO - Biological processi

  1. determination of left/right symmetry Source: MGI
  2. embryonic axis specification Source: MGI
  3. floor plate development Source: MGI
  4. forebrain development Source: MGI
  5. heart looping Source: MGI
  6. in utero embryonic development Source: MGI
  7. multicellular organism growth Source: MGI
  8. negative regulation of apoptotic process Source: MGI
  9. neural tube closure Source: MGI
  10. neural tube development Source: MGI
  11. notochord development Source: MGI
  12. smoothened signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
SCL-interrupting locus protein homolog
Gene namesi
Name:Stil
Synonyms:Sil
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:107477. Stil.

Subcellular locationi

Cytoplasmcytosol Inferred

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Death during embryonic development between days 8.5 and 10.5. Embryos are reduced in size and display delayed development. They have axial midline defects, and randomized cardiac looping. The midline sonic hedgehog signaling is blocked in these mice.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi574 – 5741T → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-643; A-656; A-664; A-686; A-699 and A-760. 1 Publication
Mutagenesisi643 – 6431S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-656; A-664; A-686; A-699 and A-760. 1 Publication
Mutagenesisi656 – 6561S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-664; A-686; A-699 and A-760. 1 Publication
Mutagenesisi664 – 6641S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-686; A-699 and A-760. 1 Publication
Mutagenesisi686 – 6861T → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-699 and A-760. 1 Publication
Mutagenesisi699 – 6991S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-686 and A-760. 1 Publication
Mutagenesisi760 – 7601S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-686 and A-699. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12621262SCL-interrupting locus protein homologPRO_0000271333Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei733 – 7331Phosphoserine By similarity
Modified residuei1110 – 11101Phosphoserine By similarity

Post-translational modificationi

Phosphorylated following the activation of the mitotic checkpoint Inferred.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ60988.
PRIDEiQ60988.

PTM databases

PhosphoSiteiQ60988.

Expressioni

Tissue specificityi

Ubiquitously expressed in adult and fetal tissues. Highly expressed in hematopoietic tissues such as thymus, bone marrow and spleen.2 Publications

Inductioni

Down-regulated during cell terminal differentiation. Accumulates during G2 phase and falls at completion of the cell cycle.

Gene expression databases

CleanExiMM_STIL.
GenevestigatoriQ60988.

Interactioni

Subunit structurei

Interacts with PIN1 via its WW domain. This interaction is dependent on Stil mitotic phosphorylation.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030490.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni567 – 760194PIN1-bindingAdd
BLAST

Phylogenomic databases

eggNOGiNOG39781.
HOGENOMiHOG000231284.
HOVERGENiHBG079548.
InParanoidiQ60988.
KOiK16724.
PhylomeDBiQ60988.

Family and domain databases

InterProiIPR026123. Sil.
[Graphical view]
PANTHERiPTHR15128. PTHR15128. 1 hit.
PfamiPF15253. STIL_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60988-1 [UniParc]FASTAAdd to Basket

« Hide

MNTRFPSSKM VPFHFPPSKL ALWNPMPIGE CIYLHLSYYR KPKLMVTEKA     50
IRLAYRHAKQ NKKNVPCFLL GSLTVDEDEE GVTLTIDRFD PGREIPECLE 100
RTPTASLPGD FLIPCRVHIQ GLGSRDVIVH NADDFSSALK ALQYHVCSKD 150
FLDCGKLLCL RAQITPRESL DGVDFNLQWT AVTLANSFKC VPVKPIPIIP 200
TALARNLSSN LNISQVQGTY KHGYITMDET RKLLLLLQSD PKVSSLPLVG 250
IWLAGIIHVY SPQVWACCLR YMFSSSIQER VFSESGNFII VLYSLTHKEP 300
EFYECLPCES RTPDLQFQLL TNKETLHLFN NVEPSGKNPI HFELSAESQD 350
AEAEAEVLSK ISKTLPVKRS SQKVSPGKIP INKHDTDLED EDFSPRPIPS 400
PHPVSQKISK VQPSVPELSL VLDNNFTESS NQSNPLEMMT VENPLLIKPS 450
QPELCDAKHS SEATTGEPFR RGPTNQLSQD TALRQSRGKQ SSTCKKESLQ 500
FRNTNAKPSL SVPSPDVAEK LQAVSAGSMQ KEDYPVRPST LDSRQPSLAP 550
QAQPHNLVFS THNSTRPMEL QVPTPSLPSY YPTNVCSCCQ HHGHIQYSTI 600
NSWQGNTVGS IQDLRSESLP KHAFFHSSGC PSLCPNAIYS SSSPVSMKQG 650
GMGAYSPHSN GEPSPVAGPS HVDSCVPHPC AMCMHTPNTA PDNGMMGLSP 700
DAYRFVTEQD RQLRLLQAQI QRLLEAQSLD PGSHKTVATM EDTVKAARQM 750
ELVSMEAQSS PGLHMRKSVS IAVSTGASLF WNAAGDDQEP DSQPKQDDTK 800
ISSEDMNFSV DINNEATSLP GSASSLKAVD IPSFEESNLA VEEVNQPLPE 850
SNSSSEQSKE PGVPVFFPNA LLAESVSMCL QTAPTEGASN STELPQGTKD 900
EPYRPSDNQK IYQDLLGQVN HLLSNASQET EEPPTKAVVT NHECAKTQNT 950
HHARKKRHNS GLVDKDCVLS ATIKQLRSLG VKIDSPTKVK KNEQKVDHAS 1000
VLACISPEAV ISGLNYMSFG NVGMSSLSPT GVDLSMEANA IALKYLSENQ 1050
LSQLSLARSK QNNGDSSVGL LHINSDRSTV GLSLVSPSNM SFATKKYMKR 1100
YGLLQSSDNS EDEEEPPSHA DSESDHVLNR NPACRPVQCG HEKEPSWNAC 1150
EIAQCSDCGS ADTRTDVPVL RNITNQAVQP RATEHLNEDS AISLRNLKPN 1200
PAMNLRTGKA EFTHHPEKEN ERDIAVFPGT LPSPETLKQM NSMDSVGTFL 1250
DVKRLRQLPK LF 1262
Length:1,262
Mass (Da):138,757
Last modified:November 1, 1996 - v1
Checksum:iEF2678A1445F9743
GO

Sequence cautioni

The sequence BAC25593.1 differs from that shown. Reason: Frameshift at position 1057.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti374 – 3741V → L in BAC33619. 1 Publication
Sequence conflicti686 – 6861T → M in BAC25593. 1 Publication
Sequence conflicti686 – 6861T → M in BAC33619. 1 Publication
Sequence conflicti740 – 7401M → V in BAC25593. 1 Publication
Sequence conflicti740 – 7401M → V in BAC33619. 1 Publication
Sequence conflicti748 – 7481R → K in BAC25593. 1 Publication
Sequence conflicti748 – 7481R → K in BAC33619. 1 Publication
Sequence conflicti942 – 9421H → R in BAC25593. 1 Publication
Sequence conflicti942 – 9421H → R in BAC33619. 1 Publication
Sequence conflicti1060 – 10601K → N in AAH04585. 1 Publication
Sequence conflicti1137 – 11371V → A in AAH04585. 1 Publication
Sequence conflicti1164 – 11641R → C in AAH04585. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U36778 mRNA. Translation: AAC52386.1.
AJ297131 Genomic DNA. Translation: CAC14001.1.
AK019471 mRNA. Translation: BAC25593.1. Frameshift.
AK049223 mRNA. Translation: BAC33619.1.
BC004585 mRNA. Translation: AAH04585.1.
BC049865 mRNA. Translation: AAH49865.1. Sequence problems.
CCDSiCCDS18485.1.
RefSeqiNP_033211.2. NM_009185.3.
UniGeneiMm.3988.

Genome annotation databases

GeneIDi20460.
KEGGimmu:20460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U36778 mRNA. Translation: AAC52386.1 .
AJ297131 Genomic DNA. Translation: CAC14001.1 .
AK019471 mRNA. Translation: BAC25593.1 . Frameshift.
AK049223 mRNA. Translation: BAC33619.1 .
BC004585 mRNA. Translation: AAH04585.1 .
BC049865 mRNA. Translation: AAH49865.1 . Sequence problems.
CCDSi CCDS18485.1.
RefSeqi NP_033211.2. NM_009185.3.
UniGenei Mm.3988.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000030490.

PTM databases

PhosphoSitei Q60988.

Proteomic databases

PaxDbi Q60988.
PRIDEi Q60988.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 20460.
KEGGi mmu:20460.

Organism-specific databases

CTDi 6491.
MGIi MGI:107477. Stil.

Phylogenomic databases

eggNOGi NOG39781.
HOGENOMi HOG000231284.
HOVERGENi HBG079548.
InParanoidi Q60988.
KOi K16724.
PhylomeDBi Q60988.

Miscellaneous databases

NextBioi 298542.
PROi Q60988.
SOURCEi Search...

Gene expression databases

CleanExi MM_STIL.
Genevestigatori Q60988.

Family and domain databases

InterProi IPR026123. Sil.
[Graphical view ]
PANTHERi PTHR15128. PTHR15128. 1 hit.
Pfami PF15253. STIL_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a murine SIL gene."
    Collazo-Garcia N., Scherer P., Aplan P.D.
    Genomics 30:506-513(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
    Strain: C57BL/6 X CBA.
  2. "Long-range comparison of human and mouse SCL loci: localized regions of sensitivity to restriction endonucleases correspond precisely with peaks of conserved noncoding sequences."
    Goettgens B., Gilbert J.G.R., Barton L.M., Grafham D., Rogers J., Bentley D.R., Green A.R.
    Genome Res. 11:87-97(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1094 AND 449-1262.
    Strain: C57BL/6J.
    Tissue: Skin.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-957 AND 1056-1262.
    Strain: Czech II and FVB/N.
    Tissue: Mammary gland.
  5. "Structural characterization of SIL, a gene frequently disrupted in T-cell acute lymphoblastic leukemia."
    Aplan P.D., Lombardi D.P., Kirsch I.R.
    Mol. Cell. Biol. 11:5462-5469(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "The SIL gene is required for mouse embryonic axial development and left-right specification."
    Izraeli S., Lowe L.A., Bertness V.L., Good D.J., Dorward D.W., Kirsch I.R., Kuehn M.R.
    Nature 399:691-694(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "Genetic evidence that Sil is required for the Sonic Hedgehog response pathway."
    Izraeli S., Lowe L.A., Bertness V.L., Campaner S., Hahn H., Kirsch I.R., Kuehn M.R.
    Genesis 31:72-77(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Sil phosphorylation in a Pin1 binding domain affects the duration of the spindle checkpoint."
    Campaner S., Kaldis P., Izraeli S., Kirsch I.R.
    Mol. Cell. Biol. 25:6660-6672(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PIN1, REGION, MUTAGENESIS OF THR-574; SER-643; SER-656; SER-664; THR-686; SER-699 AND SER-760, PHOSPHORYLATION.

Entry informationi

Entry nameiSTIL_MOUSE
AccessioniPrimary (citable) accession number: Q60988
Secondary accession number(s): Q80VK7
, Q8C7U6, Q8CEL7, Q99KL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

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