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Q60988

- STIL_MOUSE

UniProt

Q60988 - STIL_MOUSE

Protein

SCL-interrupting locus protein homolog

Gene

Stil

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1.3 Publications

    GO - Biological processi

    1. determination of left/right symmetry Source: MGI
    2. embryonic axis specification Source: MGI
    3. floor plate development Source: MGI
    4. forebrain development Source: MGI
    5. heart looping Source: MGI
    6. in utero embryonic development Source: MGI
    7. multicellular organism growth Source: MGI
    8. negative regulation of apoptotic process Source: MGI
    9. neural tube closure Source: MGI
    10. neural tube development Source: MGI
    11. notochord development Source: MGI
    12. smoothened signaling pathway Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SCL-interrupting locus protein homolog
    Gene namesi
    Name:Stil
    Synonyms:Sil
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:107477. Stil.

    Subcellular locationi

    Cytoplasmcytosol Curated

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Death during embryonic development between days 8.5 and 10.5. Embryos are reduced in size and display delayed development. They have axial midline defects, and randomized cardiac looping. The midline sonic hedgehog signaling is blocked in these mice.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi574 – 5741T → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-643; A-656; A-664; A-686; A-699 and A-760. 1 Publication
    Mutagenesisi643 – 6431S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-656; A-664; A-686; A-699 and A-760. 1 Publication
    Mutagenesisi656 – 6561S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-664; A-686; A-699 and A-760. 1 Publication
    Mutagenesisi664 – 6641S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-686; A-699 and A-760. 1 Publication
    Mutagenesisi686 – 6861T → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-699 and A-760. 1 Publication
    Mutagenesisi699 – 6991S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-686 and A-760. 1 Publication
    Mutagenesisi760 – 7601S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-686 and A-699. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12621262SCL-interrupting locus protein homologPRO_0000271333Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei733 – 7331PhosphoserineBy similarity
    Modified residuei1110 – 11101PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated following the activation of the mitotic checkpoint.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ60988.
    PRIDEiQ60988.

    PTM databases

    PhosphoSiteiQ60988.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed in adult and fetal tissues. Highly expressed in hematopoietic tissues such as thymus, bone marrow and spleen.2 Publications

    Inductioni

    Down-regulated during cell terminal differentiation. Accumulates during G2 phase and falls at completion of the cell cycle.

    Gene expression databases

    CleanExiMM_STIL.
    GenevestigatoriQ60988.

    Interactioni

    Subunit structurei

    Interacts with PIN1 via its WW domain. This interaction is dependent on Stil mitotic phosphorylation.1 Publication

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000030490.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni567 – 760194PIN1-bindingAdd
    BLAST

    Phylogenomic databases

    eggNOGiNOG39781.
    HOGENOMiHOG000231284.
    HOVERGENiHBG079548.
    InParanoidiQ60988.
    KOiK16724.
    PhylomeDBiQ60988.

    Family and domain databases

    InterProiIPR026123. Sil.
    [Graphical view]
    PANTHERiPTHR15128. PTHR15128. 1 hit.
    PfamiPF15253. STIL_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60988-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTRFPSSKM VPFHFPPSKL ALWNPMPIGE CIYLHLSYYR KPKLMVTEKA     50
    IRLAYRHAKQ NKKNVPCFLL GSLTVDEDEE GVTLTIDRFD PGREIPECLE 100
    RTPTASLPGD FLIPCRVHIQ GLGSRDVIVH NADDFSSALK ALQYHVCSKD 150
    FLDCGKLLCL RAQITPRESL DGVDFNLQWT AVTLANSFKC VPVKPIPIIP 200
    TALARNLSSN LNISQVQGTY KHGYITMDET RKLLLLLQSD PKVSSLPLVG 250
    IWLAGIIHVY SPQVWACCLR YMFSSSIQER VFSESGNFII VLYSLTHKEP 300
    EFYECLPCES RTPDLQFQLL TNKETLHLFN NVEPSGKNPI HFELSAESQD 350
    AEAEAEVLSK ISKTLPVKRS SQKVSPGKIP INKHDTDLED EDFSPRPIPS 400
    PHPVSQKISK VQPSVPELSL VLDNNFTESS NQSNPLEMMT VENPLLIKPS 450
    QPELCDAKHS SEATTGEPFR RGPTNQLSQD TALRQSRGKQ SSTCKKESLQ 500
    FRNTNAKPSL SVPSPDVAEK LQAVSAGSMQ KEDYPVRPST LDSRQPSLAP 550
    QAQPHNLVFS THNSTRPMEL QVPTPSLPSY YPTNVCSCCQ HHGHIQYSTI 600
    NSWQGNTVGS IQDLRSESLP KHAFFHSSGC PSLCPNAIYS SSSPVSMKQG 650
    GMGAYSPHSN GEPSPVAGPS HVDSCVPHPC AMCMHTPNTA PDNGMMGLSP 700
    DAYRFVTEQD RQLRLLQAQI QRLLEAQSLD PGSHKTVATM EDTVKAARQM 750
    ELVSMEAQSS PGLHMRKSVS IAVSTGASLF WNAAGDDQEP DSQPKQDDTK 800
    ISSEDMNFSV DINNEATSLP GSASSLKAVD IPSFEESNLA VEEVNQPLPE 850
    SNSSSEQSKE PGVPVFFPNA LLAESVSMCL QTAPTEGASN STELPQGTKD 900
    EPYRPSDNQK IYQDLLGQVN HLLSNASQET EEPPTKAVVT NHECAKTQNT 950
    HHARKKRHNS GLVDKDCVLS ATIKQLRSLG VKIDSPTKVK KNEQKVDHAS 1000
    VLACISPEAV ISGLNYMSFG NVGMSSLSPT GVDLSMEANA IALKYLSENQ 1050
    LSQLSLARSK QNNGDSSVGL LHINSDRSTV GLSLVSPSNM SFATKKYMKR 1100
    YGLLQSSDNS EDEEEPPSHA DSESDHVLNR NPACRPVQCG HEKEPSWNAC 1150
    EIAQCSDCGS ADTRTDVPVL RNITNQAVQP RATEHLNEDS AISLRNLKPN 1200
    PAMNLRTGKA EFTHHPEKEN ERDIAVFPGT LPSPETLKQM NSMDSVGTFL 1250
    DVKRLRQLPK LF 1262
    Length:1,262
    Mass (Da):138,757
    Last modified:November 1, 1996 - v1
    Checksum:iEF2678A1445F9743
    GO

    Sequence cautioni

    The sequence BAC25593.1 differs from that shown. Reason: Frameshift at position 1057.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti374 – 3741V → L in BAC33619. (PubMed:16141072)Curated
    Sequence conflicti686 – 6861T → M in BAC25593. (PubMed:16141072)Curated
    Sequence conflicti686 – 6861T → M in BAC33619. (PubMed:16141072)Curated
    Sequence conflicti740 – 7401M → V in BAC25593. (PubMed:16141072)Curated
    Sequence conflicti740 – 7401M → V in BAC33619. (PubMed:16141072)Curated
    Sequence conflicti748 – 7481R → K in BAC25593. (PubMed:16141072)Curated
    Sequence conflicti748 – 7481R → K in BAC33619. (PubMed:16141072)Curated
    Sequence conflicti942 – 9421H → R in BAC25593. (PubMed:16141072)Curated
    Sequence conflicti942 – 9421H → R in BAC33619. (PubMed:16141072)Curated
    Sequence conflicti1060 – 10601K → N in AAH04585. (PubMed:15489334)Curated
    Sequence conflicti1137 – 11371V → A in AAH04585. (PubMed:15489334)Curated
    Sequence conflicti1164 – 11641R → C in AAH04585. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36778 mRNA. Translation: AAC52386.1.
    AJ297131 Genomic DNA. Translation: CAC14001.1.
    AK019471 mRNA. Translation: BAC25593.1. Frameshift.
    AK049223 mRNA. Translation: BAC33619.1.
    BC004585 mRNA. Translation: AAH04585.1.
    BC049865 mRNA. Translation: AAH49865.1. Sequence problems.
    CCDSiCCDS18485.1.
    RefSeqiNP_033211.2. NM_009185.3.
    UniGeneiMm.3988.

    Genome annotation databases

    GeneIDi20460.
    KEGGimmu:20460.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36778 mRNA. Translation: AAC52386.1 .
    AJ297131 Genomic DNA. Translation: CAC14001.1 .
    AK019471 mRNA. Translation: BAC25593.1 . Frameshift.
    AK049223 mRNA. Translation: BAC33619.1 .
    BC004585 mRNA. Translation: AAH04585.1 .
    BC049865 mRNA. Translation: AAH49865.1 . Sequence problems.
    CCDSi CCDS18485.1.
    RefSeqi NP_033211.2. NM_009185.3.
    UniGenei Mm.3988.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000030490.

    PTM databases

    PhosphoSitei Q60988.

    Proteomic databases

    PaxDbi Q60988.
    PRIDEi Q60988.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 20460.
    KEGGi mmu:20460.

    Organism-specific databases

    CTDi 6491.
    MGIi MGI:107477. Stil.

    Phylogenomic databases

    eggNOGi NOG39781.
    HOGENOMi HOG000231284.
    HOVERGENi HBG079548.
    InParanoidi Q60988.
    KOi K16724.
    PhylomeDBi Q60988.

    Miscellaneous databases

    NextBioi 298542.
    PROi Q60988.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_STIL.
    Genevestigatori Q60988.

    Family and domain databases

    InterProi IPR026123. Sil.
    [Graphical view ]
    PANTHERi PTHR15128. PTHR15128. 1 hit.
    Pfami PF15253. STIL_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a murine SIL gene."
      Collazo-Garcia N., Scherer P., Aplan P.D.
      Genomics 30:506-513(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
      Strain: C57BL/6 X CBA.
    2. "Long-range comparison of human and mouse SCL loci: localized regions of sensitivity to restriction endonucleases correspond precisely with peaks of conserved noncoding sequences."
      Goettgens B., Gilbert J.G.R., Barton L.M., Grafham D., Rogers J., Bentley D.R., Green A.R.
      Genome Res. 11:87-97(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1094 AND 449-1262.
      Strain: C57BL/6J.
      Tissue: Skin.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-957 AND 1056-1262.
      Strain: Czech II and FVB/N.
      Tissue: Mammary gland.
    5. "Structural characterization of SIL, a gene frequently disrupted in T-cell acute lymphoblastic leukemia."
      Aplan P.D., Lombardi D.P., Kirsch I.R.
      Mol. Cell. Biol. 11:5462-5469(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "The SIL gene is required for mouse embryonic axial development and left-right specification."
      Izraeli S., Lowe L.A., Bertness V.L., Good D.J., Dorward D.W., Kirsch I.R., Kuehn M.R.
      Nature 399:691-694(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    7. "Genetic evidence that Sil is required for the Sonic Hedgehog response pathway."
      Izraeli S., Lowe L.A., Bertness V.L., Campaner S., Hahn H., Kirsch I.R., Kuehn M.R.
      Genesis 31:72-77(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Sil phosphorylation in a Pin1 binding domain affects the duration of the spindle checkpoint."
      Campaner S., Kaldis P., Izraeli S., Kirsch I.R.
      Mol. Cell. Biol. 25:6660-6672(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PIN1, REGION, MUTAGENESIS OF THR-574; SER-643; SER-656; SER-664; THR-686; SER-699 AND SER-760, PHOSPHORYLATION.

    Entry informationi

    Entry nameiSTIL_MOUSE
    AccessioniPrimary (citable) accession number: Q60988
    Secondary accession number(s): Q80VK7
    , Q8C7U6, Q8CEL7, Q99KL4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3