Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q60988 (STIL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SCL-interrupting locus protein homolog
Gene names
Name:Stil
Synonyms:Sil
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1262 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1. Ref.1 Ref.6 Ref.7

Subunit structure

Interacts with PIN1 via its WW domain. This interaction is dependent on Stil mitotic phosphorylation. Ref.8

Subcellular location

Cytoplasmcytosol Probable.

Tissue specificity

Ubiquitously expressed in adult and fetal tissues. Highly expressed in hematopoietic tissues such as thymus, bone marrow and spleen. Ref.1 Ref.5

Induction

Down-regulated during cell terminal differentiation. Accumulates during G2 phase and falls at completion of the cell cycle.

Post-translational modification

Phosphorylated following the activation of the mitotic checkpoint Probable. Ref.8

Disruption phenotype

Death during embryonic development between days 8.5 and 10.5. Embryos are reduced in size and display delayed development. They have axial midline defects, and randomized cardiac looping. The midline sonic hedgehog signaling is blocked in these mice. Ref.6

Sequence caution

The sequence BAC25593.1 differs from that shown. Reason: Frameshift at position 1057.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12621262SCL-interrupting locus protein homolog
PRO_0000271333

Regions

Region567 – 760194PIN1-binding

Amino acid modifications

Modified residue7331Phosphoserine By similarity
Modified residue11101Phosphoserine By similarity

Experimental info

Mutagenesis5741T → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-643; A-656; A-664; A-686; A-699 and A-760. Ref.8
Mutagenesis6431S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-656; A-664; A-686; A-699 and A-760. Ref.8
Mutagenesis6561S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-664; A-686; A-699 and A-760. Ref.8
Mutagenesis6641S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-686; A-699 and A-760. Ref.8
Mutagenesis6861T → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-699 and A-760. Ref.8
Mutagenesis6991S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-686 and A-760. Ref.8
Mutagenesis7601S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-686 and A-699. Ref.8
Sequence conflict3741V → L in BAC33619. Ref.3
Sequence conflict6861T → M in BAC25593. Ref.3
Sequence conflict6861T → M in BAC33619. Ref.3
Sequence conflict7401M → V in BAC25593. Ref.3
Sequence conflict7401M → V in BAC33619. Ref.3
Sequence conflict7481R → K in BAC25593. Ref.3
Sequence conflict7481R → K in BAC33619. Ref.3
Sequence conflict9421H → R in BAC25593. Ref.3
Sequence conflict9421H → R in BAC33619. Ref.3
Sequence conflict10601K → N in AAH04585. Ref.4
Sequence conflict11371V → A in AAH04585. Ref.4
Sequence conflict11641R → C in AAH04585. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q60988 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EF2678A1445F9743

FASTA1,262138,757
        10         20         30         40         50         60 
MNTRFPSSKM VPFHFPPSKL ALWNPMPIGE CIYLHLSYYR KPKLMVTEKA IRLAYRHAKQ 

        70         80         90        100        110        120 
NKKNVPCFLL GSLTVDEDEE GVTLTIDRFD PGREIPECLE RTPTASLPGD FLIPCRVHIQ 

       130        140        150        160        170        180 
GLGSRDVIVH NADDFSSALK ALQYHVCSKD FLDCGKLLCL RAQITPRESL DGVDFNLQWT 

       190        200        210        220        230        240 
AVTLANSFKC VPVKPIPIIP TALARNLSSN LNISQVQGTY KHGYITMDET RKLLLLLQSD 

       250        260        270        280        290        300 
PKVSSLPLVG IWLAGIIHVY SPQVWACCLR YMFSSSIQER VFSESGNFII VLYSLTHKEP 

       310        320        330        340        350        360 
EFYECLPCES RTPDLQFQLL TNKETLHLFN NVEPSGKNPI HFELSAESQD AEAEAEVLSK 

       370        380        390        400        410        420 
ISKTLPVKRS SQKVSPGKIP INKHDTDLED EDFSPRPIPS PHPVSQKISK VQPSVPELSL 

       430        440        450        460        470        480 
VLDNNFTESS NQSNPLEMMT VENPLLIKPS QPELCDAKHS SEATTGEPFR RGPTNQLSQD 

       490        500        510        520        530        540 
TALRQSRGKQ SSTCKKESLQ FRNTNAKPSL SVPSPDVAEK LQAVSAGSMQ KEDYPVRPST 

       550        560        570        580        590        600 
LDSRQPSLAP QAQPHNLVFS THNSTRPMEL QVPTPSLPSY YPTNVCSCCQ HHGHIQYSTI 

       610        620        630        640        650        660 
NSWQGNTVGS IQDLRSESLP KHAFFHSSGC PSLCPNAIYS SSSPVSMKQG GMGAYSPHSN 

       670        680        690        700        710        720 
GEPSPVAGPS HVDSCVPHPC AMCMHTPNTA PDNGMMGLSP DAYRFVTEQD RQLRLLQAQI 

       730        740        750        760        770        780 
QRLLEAQSLD PGSHKTVATM EDTVKAARQM ELVSMEAQSS PGLHMRKSVS IAVSTGASLF 

       790        800        810        820        830        840 
WNAAGDDQEP DSQPKQDDTK ISSEDMNFSV DINNEATSLP GSASSLKAVD IPSFEESNLA 

       850        860        870        880        890        900 
VEEVNQPLPE SNSSSEQSKE PGVPVFFPNA LLAESVSMCL QTAPTEGASN STELPQGTKD 

       910        920        930        940        950        960 
EPYRPSDNQK IYQDLLGQVN HLLSNASQET EEPPTKAVVT NHECAKTQNT HHARKKRHNS 

       970        980        990       1000       1010       1020 
GLVDKDCVLS ATIKQLRSLG VKIDSPTKVK KNEQKVDHAS VLACISPEAV ISGLNYMSFG 

      1030       1040       1050       1060       1070       1080 
NVGMSSLSPT GVDLSMEANA IALKYLSENQ LSQLSLARSK QNNGDSSVGL LHINSDRSTV 

      1090       1100       1110       1120       1130       1140 
GLSLVSPSNM SFATKKYMKR YGLLQSSDNS EDEEEPPSHA DSESDHVLNR NPACRPVQCG 

      1150       1160       1170       1180       1190       1200 
HEKEPSWNAC EIAQCSDCGS ADTRTDVPVL RNITNQAVQP RATEHLNEDS AISLRNLKPN 

      1210       1220       1230       1240       1250       1260 
PAMNLRTGKA EFTHHPEKEN ERDIAVFPGT LPSPETLKQM NSMDSVGTFL DVKRLRQLPK 


LF 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a murine SIL gene."
Collazo-Garcia N., Scherer P., Aplan P.D.
Genomics 30:506-513(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
Strain: C57BL/6 X CBA.
[2]"Long-range comparison of human and mouse SCL loci: localized regions of sensitivity to restriction endonucleases correspond precisely with peaks of conserved noncoding sequences."
Goettgens B., Gilbert J.G.R., Barton L.M., Grafham D., Rogers J., Bentley D.R., Green A.R.
Genome Res. 11:87-97(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1094 AND 449-1262.
Strain: C57BL/6J.
Tissue: Skin.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-957 AND 1056-1262.
Strain: Czech II and FVB/N.
Tissue: Mammary gland.
[5]"Structural characterization of SIL, a gene frequently disrupted in T-cell acute lymphoblastic leukemia."
Aplan P.D., Lombardi D.P., Kirsch I.R.
Mol. Cell. Biol. 11:5462-5469(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"The SIL gene is required for mouse embryonic axial development and left-right specification."
Izraeli S., Lowe L.A., Bertness V.L., Good D.J., Dorward D.W., Kirsch I.R., Kuehn M.R.
Nature 399:691-694(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]"Genetic evidence that Sil is required for the Sonic Hedgehog response pathway."
Izraeli S., Lowe L.A., Bertness V.L., Campaner S., Hahn H., Kirsch I.R., Kuehn M.R.
Genesis 31:72-77(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Sil phosphorylation in a Pin1 binding domain affects the duration of the spindle checkpoint."
Campaner S., Kaldis P., Izraeli S., Kirsch I.R.
Mol. Cell. Biol. 25:6660-6672(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PIN1, REGION, MUTAGENESIS OF THR-574; SER-643; SER-656; SER-664; THR-686; SER-699 AND SER-760, PHOSPHORYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U36778 mRNA. Translation: AAC52386.1.
AJ297131 Genomic DNA. Translation: CAC14001.1.
AK019471 mRNA. Translation: BAC25593.1. Frameshift.
AK049223 mRNA. Translation: BAC33619.1.
BC004585 mRNA. Translation: AAH04585.1.
BC049865 mRNA. Translation: AAH49865.1. Sequence problems.
CCDSCCDS18485.1.
RefSeqNP_033211.2. NM_009185.3.
UniGeneMm.3988.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000030490.

PTM databases

PhosphoSiteQ60988.

Proteomic databases

PaxDbQ60988.
PRIDEQ60988.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID20460.
KEGGmmu:20460.

Organism-specific databases

CTD6491.
MGIMGI:107477. Stil.

Phylogenomic databases

eggNOGNOG39781.
HOGENOMHOG000231284.
HOVERGENHBG079548.
InParanoidQ60988.
KOK16724.
PhylomeDBQ60988.

Gene expression databases

CleanExMM_STIL.
GenevestigatorQ60988.

Family and domain databases

InterProIPR026123. Sil.
[Graphical view]
PANTHERPTHR15128. PTHR15128. 1 hit.
PfamPF15253. STIL_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298542.
PROQ60988.
SOURCESearch...

Entry information

Entry nameSTIL_MOUSE
AccessionPrimary (citable) accession number: Q60988
Secondary accession number(s): Q80VK7 expand/collapse secondary AC list , Q8C7U6, Q8CEL7, Q99KL4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot