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Protein

SCL-interrupting locus protein homolog

Gene

Stil

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1.3 Publications

GO - Biological processi

  1. centrosome duplication Source: MGI
  2. cilium morphogenesis Source: MGI
  3. determination of left/right symmetry Source: MGI
  4. embryonic axis specification Source: MGI
  5. floor plate development Source: MGI
  6. forebrain development Source: MGI
  7. heart looping Source: MGI
  8. in utero embryonic development Source: MGI
  9. mitotic spindle organization Source: MGI
  10. multicellular organism growth Source: MGI
  11. negative regulation of apoptotic process Source: MGI
  12. neural tube closure Source: MGI
  13. neural tube development Source: MGI
  14. notochord development Source: MGI
  15. positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
  16. protein localization to centrosome Source: MGI
  17. smoothened signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
SCL-interrupting locus protein homolog
Gene namesi
Name:Stil
Synonyms:Sil
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:107477. Stil.

Subcellular locationi

Cytoplasmcytosol Curated

GO - Cellular componenti

  1. centriole Source: MGI
  2. centrosome Source: MGI
  3. cytoplasm Source: MGI
  4. cytosol Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Death during embryonic development between days 8.5 and 10.5. Embryos are reduced in size and display delayed development. They have axial midline defects, and randomized cardiac looping. The midline sonic hedgehog signaling is blocked in these mice.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi574 – 5741T → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-643; A-656; A-664; A-686; A-699 and A-760. 1 Publication
Mutagenesisi643 – 6431S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-656; A-664; A-686; A-699 and A-760. 1 Publication
Mutagenesisi656 – 6561S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-664; A-686; A-699 and A-760. 1 Publication
Mutagenesisi664 – 6641S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-686; A-699 and A-760. 1 Publication
Mutagenesisi686 – 6861T → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-699 and A-760. 1 Publication
Mutagenesisi699 – 6991S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-686 and A-760. 1 Publication
Mutagenesisi760 – 7601S → A: Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-686 and A-699. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12621262SCL-interrupting locus protein homologPRO_0000271333Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei733 – 7331PhosphoserineBy similarity
Modified residuei1110 – 11101PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated following the activation of the mitotic checkpoint.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ60988.
PRIDEiQ60988.

PTM databases

PhosphoSiteiQ60988.

Expressioni

Tissue specificityi

Ubiquitously expressed in adult and fetal tissues. Highly expressed in hematopoietic tissues such as thymus, bone marrow and spleen.2 Publications

Inductioni

Down-regulated during cell terminal differentiation. Accumulates during G2 phase and falls at completion of the cell cycle.

Gene expression databases

CleanExiMM_STIL.
GenevestigatoriQ60988.

Interactioni

Subunit structurei

Interacts with PIN1 via its WW domain. This interaction is dependent on Stil mitotic phosphorylation.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030490.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni567 – 760194PIN1-bindingAdd
BLAST

Phylogenomic databases

eggNOGiNOG39781.
HOGENOMiHOG000231284.
HOVERGENiHBG079548.
InParanoidiQ60988.
KOiK16724.
PhylomeDBiQ60988.

Family and domain databases

InterProiIPR026123. Sil.
[Graphical view]
PANTHERiPTHR15128. PTHR15128. 1 hit.
PfamiPF15253. STIL_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60988-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTRFPSSKM VPFHFPPSKL ALWNPMPIGE CIYLHLSYYR KPKLMVTEKA
60 70 80 90 100
IRLAYRHAKQ NKKNVPCFLL GSLTVDEDEE GVTLTIDRFD PGREIPECLE
110 120 130 140 150
RTPTASLPGD FLIPCRVHIQ GLGSRDVIVH NADDFSSALK ALQYHVCSKD
160 170 180 190 200
FLDCGKLLCL RAQITPRESL DGVDFNLQWT AVTLANSFKC VPVKPIPIIP
210 220 230 240 250
TALARNLSSN LNISQVQGTY KHGYITMDET RKLLLLLQSD PKVSSLPLVG
260 270 280 290 300
IWLAGIIHVY SPQVWACCLR YMFSSSIQER VFSESGNFII VLYSLTHKEP
310 320 330 340 350
EFYECLPCES RTPDLQFQLL TNKETLHLFN NVEPSGKNPI HFELSAESQD
360 370 380 390 400
AEAEAEVLSK ISKTLPVKRS SQKVSPGKIP INKHDTDLED EDFSPRPIPS
410 420 430 440 450
PHPVSQKISK VQPSVPELSL VLDNNFTESS NQSNPLEMMT VENPLLIKPS
460 470 480 490 500
QPELCDAKHS SEATTGEPFR RGPTNQLSQD TALRQSRGKQ SSTCKKESLQ
510 520 530 540 550
FRNTNAKPSL SVPSPDVAEK LQAVSAGSMQ KEDYPVRPST LDSRQPSLAP
560 570 580 590 600
QAQPHNLVFS THNSTRPMEL QVPTPSLPSY YPTNVCSCCQ HHGHIQYSTI
610 620 630 640 650
NSWQGNTVGS IQDLRSESLP KHAFFHSSGC PSLCPNAIYS SSSPVSMKQG
660 670 680 690 700
GMGAYSPHSN GEPSPVAGPS HVDSCVPHPC AMCMHTPNTA PDNGMMGLSP
710 720 730 740 750
DAYRFVTEQD RQLRLLQAQI QRLLEAQSLD PGSHKTVATM EDTVKAARQM
760 770 780 790 800
ELVSMEAQSS PGLHMRKSVS IAVSTGASLF WNAAGDDQEP DSQPKQDDTK
810 820 830 840 850
ISSEDMNFSV DINNEATSLP GSASSLKAVD IPSFEESNLA VEEVNQPLPE
860 870 880 890 900
SNSSSEQSKE PGVPVFFPNA LLAESVSMCL QTAPTEGASN STELPQGTKD
910 920 930 940 950
EPYRPSDNQK IYQDLLGQVN HLLSNASQET EEPPTKAVVT NHECAKTQNT
960 970 980 990 1000
HHARKKRHNS GLVDKDCVLS ATIKQLRSLG VKIDSPTKVK KNEQKVDHAS
1010 1020 1030 1040 1050
VLACISPEAV ISGLNYMSFG NVGMSSLSPT GVDLSMEANA IALKYLSENQ
1060 1070 1080 1090 1100
LSQLSLARSK QNNGDSSVGL LHINSDRSTV GLSLVSPSNM SFATKKYMKR
1110 1120 1130 1140 1150
YGLLQSSDNS EDEEEPPSHA DSESDHVLNR NPACRPVQCG HEKEPSWNAC
1160 1170 1180 1190 1200
EIAQCSDCGS ADTRTDVPVL RNITNQAVQP RATEHLNEDS AISLRNLKPN
1210 1220 1230 1240 1250
PAMNLRTGKA EFTHHPEKEN ERDIAVFPGT LPSPETLKQM NSMDSVGTFL
1260
DVKRLRQLPK LF
Length:1,262
Mass (Da):138,757
Last modified:November 1, 1996 - v1
Checksum:iEF2678A1445F9743
GO

Sequence cautioni

The sequence BAC25593.1 differs from that shown. Reason: Frameshift at position 1057. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti374 – 3741V → L in BAC33619 (PubMed:16141072).Curated
Sequence conflicti686 – 6861T → M in BAC25593 (PubMed:16141072).Curated
Sequence conflicti686 – 6861T → M in BAC33619 (PubMed:16141072).Curated
Sequence conflicti740 – 7401M → V in BAC25593 (PubMed:16141072).Curated
Sequence conflicti740 – 7401M → V in BAC33619 (PubMed:16141072).Curated
Sequence conflicti748 – 7481R → K in BAC25593 (PubMed:16141072).Curated
Sequence conflicti748 – 7481R → K in BAC33619 (PubMed:16141072).Curated
Sequence conflicti942 – 9421H → R in BAC25593 (PubMed:16141072).Curated
Sequence conflicti942 – 9421H → R in BAC33619 (PubMed:16141072).Curated
Sequence conflicti1060 – 10601K → N in AAH04585 (PubMed:15489334).Curated
Sequence conflicti1137 – 11371V → A in AAH04585 (PubMed:15489334).Curated
Sequence conflicti1164 – 11641R → C in AAH04585 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36778 mRNA. Translation: AAC52386.1.
AJ297131 Genomic DNA. Translation: CAC14001.1.
AK019471 mRNA. Translation: BAC25593.1. Frameshift.
AK049223 mRNA. Translation: BAC33619.1.
BC004585 mRNA. Translation: AAH04585.1.
BC049865 mRNA. Translation: AAH49865.1. Sequence problems.
CCDSiCCDS18485.1.
RefSeqiNP_033211.2. NM_009185.3.
UniGeneiMm.3988.

Genome annotation databases

GeneIDi20460.
KEGGimmu:20460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36778 mRNA. Translation: AAC52386.1.
AJ297131 Genomic DNA. Translation: CAC14001.1.
AK019471 mRNA. Translation: BAC25593.1. Frameshift.
AK049223 mRNA. Translation: BAC33619.1.
BC004585 mRNA. Translation: AAH04585.1.
BC049865 mRNA. Translation: AAH49865.1. Sequence problems.
CCDSiCCDS18485.1.
RefSeqiNP_033211.2. NM_009185.3.
UniGeneiMm.3988.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030490.

PTM databases

PhosphoSiteiQ60988.

Proteomic databases

PaxDbiQ60988.
PRIDEiQ60988.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20460.
KEGGimmu:20460.

Organism-specific databases

CTDi6491.
MGIiMGI:107477. Stil.

Phylogenomic databases

eggNOGiNOG39781.
HOGENOMiHOG000231284.
HOVERGENiHBG079548.
InParanoidiQ60988.
KOiK16724.
PhylomeDBiQ60988.

Miscellaneous databases

NextBioi298542.
PROiQ60988.
SOURCEiSearch...

Gene expression databases

CleanExiMM_STIL.
GenevestigatoriQ60988.

Family and domain databases

InterProiIPR026123. Sil.
[Graphical view]
PANTHERiPTHR15128. PTHR15128. 1 hit.
PfamiPF15253. STIL_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a murine SIL gene."
    Collazo-Garcia N., Scherer P., Aplan P.D.
    Genomics 30:506-513(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
    Strain: C57BL/6 X CBA.
  2. "Long-range comparison of human and mouse SCL loci: localized regions of sensitivity to restriction endonucleases correspond precisely with peaks of conserved noncoding sequences."
    Goettgens B., Gilbert J.G.R., Barton L.M., Grafham D., Rogers J., Bentley D.R., Green A.R.
    Genome Res. 11:87-97(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1094 AND 449-1262.
    Strain: C57BL/6J.
    Tissue: Skin.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-957 AND 1056-1262.
    Strain: Czech II and FVB/N.
    Tissue: Mammary gland.
  5. "Structural characterization of SIL, a gene frequently disrupted in T-cell acute lymphoblastic leukemia."
    Aplan P.D., Lombardi D.P., Kirsch I.R.
    Mol. Cell. Biol. 11:5462-5469(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "The SIL gene is required for mouse embryonic axial development and left-right specification."
    Izraeli S., Lowe L.A., Bertness V.L., Good D.J., Dorward D.W., Kirsch I.R., Kuehn M.R.
    Nature 399:691-694(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "Genetic evidence that Sil is required for the Sonic Hedgehog response pathway."
    Izraeli S., Lowe L.A., Bertness V.L., Campaner S., Hahn H., Kirsch I.R., Kuehn M.R.
    Genesis 31:72-77(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Sil phosphorylation in a Pin1 binding domain affects the duration of the spindle checkpoint."
    Campaner S., Kaldis P., Izraeli S., Kirsch I.R.
    Mol. Cell. Biol. 25:6660-6672(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PIN1, REGION, MUTAGENESIS OF THR-574; SER-643; SER-656; SER-664; THR-686; SER-699 AND SER-760, PHOSPHORYLATION.

Entry informationi

Entry nameiSTIL_MOUSE
AccessioniPrimary (citable) accession number: Q60988
Secondary accession number(s): Q80VK7
, Q8C7U6, Q8CEL7, Q99KL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: March 4, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.