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Q60980

- KLF3_MOUSE

UniProt

Q60980 - KLF3_MOUSE

Protein

Krueppel-like factor 3

Gene

Klf3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri259 – 28325C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri289 – 31325C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri319 – 34123C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular response to peptide Source: Ensembl
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Krueppel-like factor 3
    Alternative name(s):
    Basic krueppel-like factor
    CACCC-box-binding protein BKLF
    TEF-2
    Gene namesi
    Name:Klf3
    Synonyms:Bklf
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1342773. Klf3.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi10 – 101K → A: Reduced sumoylation levels. No effect on DNA-binding and slight reduction of transcriptional repression. Abolishes sumoylation. No effect on DNA-binding but great reduction in transcriptional repression; when associated with A-197. 1 Publication
    Mutagenesisi12 – 121E → A: Slight reduction of transcriptional repression. Great reduction of transcriptional repression; when associated with A-199. 1 Publication
    Mutagenesisi197 – 1971K → A: Reduced sumoylation levels. No effect on DNA-binding and slight reduction of transcriptional repression. Abolishes sumoylation. No effect on DNA-binding but great reduction of transcriptional repression; when associated with A-10. 1 Publication
    Mutagenesisi199 – 1991E → A: Slight reduction of transcriptional repression. Great reduction of transcriptional repression; when associated with A-199. 1 Publication
    Mutagenesisi341 – 3411H → A, D, E, N, Q or R: Little change in DNA-binding ability. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 344344Krueppel-like factor 3PRO_0000047166Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki10 – 10Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
    Modified residuei91 – 911PhosphoserineBy similarity
    Modified residuei100 – 1001PhosphoserineBy similarity
    Modified residuei110 – 1101PhosphoserineBy similarity
    Cross-linki197 – 197Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
    Modified residuei249 – 2491PhosphoserineBy similarity

    Post-translational modificationi

    Sumoylated with SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-197 is the major site.1 Publication

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ60980.
    PRIDEiQ60980.

    PTM databases

    PhosphoSiteiQ60980.

    Expressioni

    Tissue specificityi

    In 8.5 day embryos, expressed in midbrain, anterior hindbrain and ventral forebrain. In 9 day embryos, expressed throughout ventral anterior half of embryo including midbrain-hindbrain junction, ventral midbrain, diencephalon and forebrain. At 10.5 days, distribution is more widespread with expression also found in developing limb buds. Widely expressed in the adult.1 Publication

    Gene expression databases

    ArrayExpressiQ60980.
    BgeeiQ60980.
    CleanExiMM_KLF3.
    GenevestigatoriQ60980.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi200965. 1 interaction.

    Structurei

    Secondary structure

    1
    344
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi316 – 3194
    Turni322 – 3243
    Beta strandi328 – 3303
    Helixi331 – 3388
    Helixi339 – 3413

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1P7ANMR-A314-344[»]
    1U85NMR-A314-344[»]
    1U86NMR-A314-344[»]
    ProteinModelPortaliQ60980.
    SMRiQ60980. Positions 216-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ60980.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 7474Repressor domainAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi61 – 655CTBP-binding motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1 – 250250Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri259 – 28325C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri289 – 31325C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri319 – 34123C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00750000117621.
    HOGENOMiHOG000232138.
    HOVERGENiHBG003941.
    InParanoidiQ60980.
    KOiK15605.
    OMAiNSSMQVP.
    OrthoDBiEOG747PJ4.
    PhylomeDBiQ60980.
    TreeFamiTF350556.

    Family and domain databases

    Gene3Di3.30.160.60. 3 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 3 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60980-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLMFDPVPVK QEAMDPVSVS FPSNYIESMK PNKYGVIYST PLPDKFFQTP    50
    EGLTHGIQVE PVDLTVNKRG SPPAAGGSPS SLKFPSHRRA SPGLSMPSSS 100
    PPIKKYSPPS PGVQPFGVPL SMPPVMAAAL SRHGIRSPGI LPVIQPVVVQ 150
    PVPFMYTSHL QQPLMVSLSE EMDNSNSGMP VPVIESYEKP LLQKKIKIEP 200
    GIEPQRTDYY PEEMSPPLMN PVSPPQALLQ ENHPSVIVQP GKRPLPVESP 250
    DTQRKRRIHR CDYDGCNKVY TKSSHLKAHR RTHTGEKPYK CTWEGCTWKF 300
    ARSDELTRHF RKHTGIKPFQ CPDCDRSFSR SDHLALHRKR HMLV 344
    Length:344
    Mass (Da):38,561
    Last modified:November 1, 1996 - v1
    Checksum:i2BB7E3B63A7C1D88
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36340 mRNA. Translation: AAA93256.1.
    CCDSiCCDS19301.1.
    PIRiJC6100.
    RefSeqiNP_032479.1. NM_008453.5.
    XP_006503814.1. XM_006503751.1.
    UniGeneiMm.392759.
    Mm.439720.

    Genome annotation databases

    EnsembliENSMUST00000165536; ENSMUSP00000129363; ENSMUSG00000029178.
    ENSMUST00000166409; ENSMUSP00000128429; ENSMUSG00000029178.
    GeneIDi16599.
    KEGGimmu:16599.
    UCSCiuc008xmr.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36340 mRNA. Translation: AAA93256.1 .
    CCDSi CCDS19301.1.
    PIRi JC6100.
    RefSeqi NP_032479.1. NM_008453.5.
    XP_006503814.1. XM_006503751.1.
    UniGenei Mm.392759.
    Mm.439720.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1P7A NMR - A 314-344 [» ]
    1U85 NMR - A 314-344 [» ]
    1U86 NMR - A 314-344 [» ]
    ProteinModelPortali Q60980.
    SMRi Q60980. Positions 216-344.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200965. 1 interaction.

    PTM databases

    PhosphoSitei Q60980.

    Proteomic databases

    MaxQBi Q60980.
    PRIDEi Q60980.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000165536 ; ENSMUSP00000129363 ; ENSMUSG00000029178 .
    ENSMUST00000166409 ; ENSMUSP00000128429 ; ENSMUSG00000029178 .
    GeneIDi 16599.
    KEGGi mmu:16599.
    UCSCi uc008xmr.1. mouse.

    Organism-specific databases

    CTDi 51274.
    MGIi MGI:1342773. Klf3.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00750000117621.
    HOGENOMi HOG000232138.
    HOVERGENi HBG003941.
    InParanoidi Q60980.
    KOi K15605.
    OMAi NSSMQVP.
    OrthoDBi EOG747PJ4.
    PhylomeDBi Q60980.
    TreeFami TF350556.

    Miscellaneous databases

    ChiTaRSi KLF3. mouse.
    EvolutionaryTracei Q60980.
    NextBioi 290173.
    PROi Q60980.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q60980.
    Bgeei Q60980.
    CleanExi MM_KLF3.
    Genevestigatori Q60980.

    Family and domain databases

    Gene3Di 3.30.160.60. 3 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 3 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the cDNA encoding BKLF/TEF-2, a major CACCC-box-binding protein in erythroid cells and selected other cells."
      Crossley M., Whitelaw E., Perkins A., Williams G., Fujiwara Y., Orkin S.H.
      Mol. Cell. Biol. 16:1695-1705(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Strain: DBA.
      Tissue: Leukemia.
    2. "Role for SUMO modification in facilitating transcriptional repression by BKLF."
      Perdomo J., Verger A., Turner J., Crossley M.
      Mol. Cell. Biol. 25:1549-1559(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUMOYLATION AT LYS-10 AND LYS-197, FUNCTION, MUTAGENESIS OF LYS-10; GLU-12; LYS-197 AND GLU-199.
    3. "CCHX zinc finger derivatives retain the ability to bind Zn(II) and mediate protein-DNA interactions."
      Simpson R.J.Y., Cram E.D., Czolij R., Matthews J.M., Crossley M., Mackay J.P.
      J. Biol. Chem. 278:28011-28018(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 314-344 OF WILD TYPE AND MUTANT FORMS, SUBUNIT, DNA-BINDING, MUTAGENESIS OF HIS-341.

    Entry informationi

    Entry nameiKLF3_MOUSE
    AccessioniPrimary (citable) accession number: Q60980
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3