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Protein

Histone-binding protein RBBP4

Gene

Rbbp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Repressor
Biological processCell cycle, DNA replication, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-1538133 G0 and Early G1
R-MMU-212300 PRC2 methylates histones and DNA
R-MMU-2559580 Oxidative Stress Induced Senescence
R-MMU-3214841 PKMTs methylate histone lysines
R-MMU-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-MMU-6804758 Regulation of TP53 Activity through Acetylation
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-8943724 Regulation of PTEN gene transcription
R-MMU-8953750 Transcriptional Regulation by E2F6

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-binding protein RBBP4
Alternative name(s):
Chromatin assembly factor 1 subunit C
Short name:
CAF-1 subunit C
Chromatin assembly factor I p48 subunit
Short name:
CAF-I 48 kDa subunit
Short name:
CAF-I p48
Nucleosome-remodeling factor subunit RBAP48
Retinoblastoma-binding protein 4
Short name:
RBBP-4
Retinoblastoma-binding protein p48
Gene namesi
Name:Rbbp4
Synonyms:Rbap48
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1194912 Rbbp4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000511872 – 425Histone-binding protein RBBP4Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei4N6-acetyllysine; alternateBy similarity1
Cross-linki4Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki4Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei110PhosphoserineBy similarity1
Modified residuei160N6-acetyllysine; alternateCombined sources1
Cross-linki160Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei355PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ60972
MaxQBiQ60972
PaxDbiQ60972
PeptideAtlasiQ60972
PRIDEiQ60972

2D gel databases

REPRODUCTION-2DPAGEiQ60972

PTM databases

iPTMnetiQ60972
PhosphoSitePlusiQ60972
SwissPalmiQ60972

Expressioni

Tissue specificityi

Higher levels in brain, thymus, lung, spleen, kidney, testis, and ovary/uterus; lower levels in heart, liver, and muscle.1 Publication

Gene expression databases

BgeeiENSMUSG00000057236
CleanExiMM_RBBP4
ExpressionAtlasiQ60972 baseline and differential
GenevisibleiQ60972 MM

Interactioni

Subunit structurei

Binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Subunit of the chromatin assembly factor 1 (CAF-1) complex, which is composed of RBBP4, CHAF1B and CHAF1A. Subunit of the core histone deacetylase (HDAC) complex, which is composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core HDAC complex associates with SIN3A, ARID4B/SAP180, SAP18, SAP30, SAP130, SUDS3/SAP45 and possibly ARID4A/RBP1 and ING1 to form the SIN3 HDAC complex. The core HDAC complex may also associate with MTA2, MBD3, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylase complex (the NuRD complex). The NuRD complex may also interact with MBD3L1 and MBD3L2. Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. Interacts with MTA1. Subunit of the PRC2/EED-EZH2 complex, which is composed of at least EED, EZH2, RBBP4, RBBP7 and SUZ12. The PRC2/EED-EZH2 complex may also associate with HDAC1. Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. Part of the nucleosome remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SPEN/MINT. Interacts with BRCA1 (By similarity). Interacts with CREBBP, and this interaction may be enhanced by the binding of phosphorylated CREB1 to CREBBP. Interacts with SAP30, SUV39H1 and HDAC7. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1 (PubMed:28554894).By similarity6 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202816, 22 interactors
CORUMiQ60972
DIPiDIP-32855N
IntActiQ60972, 20 interactors
MINTiQ60972
STRINGi10090.ENSMUSP00000099658

Structurei

3D structure databases

ProteinModelPortaliQ60972
SMRiQ60972
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati122 – 155WD 1Add BLAST34
Repeati175 – 206WD 2Add BLAST32
Repeati225 – 256WD 3Add BLAST32
Repeati271 – 302WD 4Add BLAST32
Repeati315 – 346WD 5Add BLAST32
Repeati372 – 403WD 6Add BLAST32

Sequence similaritiesi

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0264 Eukaryota
ENOG410XNU9 LUCA
GeneTreeiENSGT00570000079069
HOGENOMiHOG000160330
HOVERGENiHBG053236
InParanoidiQ60972
KOiK10752
OMAiTNHLADF
OrthoDBiEOG091G0A20
TreeFamiTF106485

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR022052 Histone-bd_RBBP4_N
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF12265 CAF1C_H4-bd, 1 hit
PF00400 WD40, 5 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 3 hits
PS50082 WD_REPEATS_2, 5 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q60972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKEAAFDD AVEERVINEE YKIWKKNTPF LYDLVMTHAL EWPSLTAQWL
60 70 80 90 100
PDVTRPEGKD FSIHRLVLGT HTSDEQNHLV IASVQLPNDD AQFDASHYDS
110 120 130 140 150
EKGEFGGFGS VSGKIEIEIK INHEGEVNRA RYMPQNPCII ATKTPSSDVL
160 170 180 190 200
VFDYTKHPSK PDPSGECNPD LRLRGHQKEG YGLSWNPNLS GHLLSASDDH
210 220 230 240 250
TICLWDISAV PKEGKVVDAK TIFTGHTAVV EDVSWHLLHE SLFGSVADDQ
260 270 280 290 300
KLMIWDTRSN NTSKPSHSVD AHTAEVNCLS FNPYSEFILA TGSADKTVAL
310 320 330 340 350
WDLRNLKLKL HSFESHKDEI FQVQWSPHNE TILASSGTDR RLNVWDLSKI
360 370 380 390 400
GEEQSPEDAE DGPPELLFIH GGHTAKISDF SWNPNEPWVI CSVSEDNIMQ
410 420
VWQMAENIYN DEDPEGSVDP EGQGS
Length:425
Mass (Da):47,656
Last modified:July 27, 2011 - v5
Checksum:iB71E2D55A444C360
GO

Sequence cautioni

The sequence AAC52275 differs from that shown. Reason: Frameshift at position 425.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti213E → K in AAC52275 (PubMed:7503932).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35141 mRNA Translation: AAC52275.1 Frameshift.
AL607123 Genomic DNA Translation: CAM22958.1
CU234133 Genomic DNA Translation: CAQ52144.1
CH466552 Genomic DNA Translation: EDL30202.1
BC138568 mRNA Translation: AAI38569.1
BC138570 mRNA Translation: AAI38571.1
CCDSiCCDS18688.1
PIRiI49366
RefSeqiNP_033056.2, NM_009030.3
UniGeneiMm.12145

Genome annotation databases

EnsembliENSMUST00000102598; ENSMUSP00000099658; ENSMUSG00000057236
GeneIDi19646
KEGGimmu:19646
UCSCiuc008uwt.1 mouse

Similar proteinsi

Entry informationi

Entry nameiRBBP4_MOUSE
AccessioniPrimary (citable) accession number: Q60972
Secondary accession number(s): A2A875
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 173 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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