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Protein

Serine/threonine/tyrosine-interacting protein

Gene

Styx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytically inactive phosphatase. Acts as a nuclear anchor for MAPK1/MAPK3 (ERK1/ERK2). Modulates cell-fate decisions and cell migration by spatiotemporal regulation of MAPK1/MAPK3 (ERK1/ERK2) (By similarity). Seems to play a role in spermiogenesis.By similarity

Miscellaneous

Contains a Gly residue instead of a conserved Cys residue in the dsPTPase catalytic loop which renders it catalytically inactive as a phosphatase. The binding pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases.
STYX deficient male mice show, despite a normal mating behavior, a disrupted spermatid development, resulting in a >1000-fold decrease in spermatozoa production.

GO - Molecular functioni

GO - Biological processi

  • MAPK export from nucleus Source: UniProtKB
  • regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • spermatogenesis Source: MGI

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine/tyrosine-interacting protein
Alternative name(s):
Phosphoserine/threonine/tyrosine interaction protein
Protein tyrosine phosphatase-like protein
Gene namesi
Name:Styx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componentsi: Chromosome 14, Chromosome X

Organism-specific databases

MGIiMGI:1891150 Styx

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi120G → C: Confers phosphatase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000949511 – 223Serine/threonine/tyrosine-interacting proteinAdd BLAST223

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei184PhosphoserineBy similarity1
Modified residuei201PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ60969
PRIDEiQ60969

PTM databases

iPTMnetiQ60969
PhosphoSitePlusiQ60969

Expressioni

Tissue specificityi

Widely expressed with highest levels in muscle, testis and brain.

Gene expression databases

BgeeiENSMUSG00000053205
ExpressionAtlasiQ60969 baseline and differential
GenevisibleiQ60969 MM

Interactioni

Subunit structurei

Interacts with MAPK1; independently of MAPK1 phosphorylation status. Interacts with CARHSP1/Crhsp-24.By similarity

Protein-protein interaction databases

BioGridi207879, 1 interactor
STRINGi10090.ENSMUSP00000107466

Structurei

3D structure databases

ProteinModelPortaliQ60969
SMRiQ60969
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini96 – 167Tyrosine-protein phosphataseAdd BLAST72

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118853
HOGENOMiHOG000090240
HOVERGENiHBG044540
InParanoidiQ60969
KOiK18042
OMAiNVGFVHQ
OrthoDBiEOG091G0249
PhylomeDBiQ60969
TreeFamiTF350439

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q60969-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDVKLEFPS LPQCKDDAEE WTYPMRREMQ EVLPGLFLGP YSSAMKSKLP
60 70 80 90 100
ILQKHGITHI ICIRQNIEAN FIKPNFQQLF RYLVLDIADN PVENIIRFFP
110 120 130 140 150
MTKEFIDGSL QNGGKVLVHG NAGISRSAAF VIAYIMETFG MKYRDAFAYV
160 170 180 190 200
QERRFCINPN AGFVHQLQEY EAIYLAKLTI QMMSPLQIER SLAVHSGTTG
210 220
SVKRTHEEDD DFGNMQVATA QNG
Length:223
Mass (Da):25,430
Last modified:February 28, 2003 - v2
Checksum:i7D13AFEF2B66B723
GO
Isoform 2 (identifier: Q60969-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     169-223: EYEAIYLAKL...NMQVATAQNG → LWLSWNSARS...TSKNKQTNNS

Show »
Length:204
Mass (Da):23,550
Checksum:i59F79FF3E66EC8F7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11L → V in AAA87036 (PubMed:7592916).Curated1
Sequence conflicti11L → V in AAA87037 (PubMed:7592916).Curated1
Sequence conflicti81R → T in BAC32931 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005174169 – 223EYEAI…TAQNG → LWLSWNSARSAPLPLKQRQV YHCAFKTSKNKQTNNS in isoform 2. 1 PublicationAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34973 mRNA Translation: AAA87036.1
U34973 mRNA Translation: AAA87037.1
AK002822 mRNA Translation: BAB22384.1
AK030122 mRNA Translation: BAC26793.1
AK046970 mRNA Translation: BAC32931.1
AK166043 mRNA Translation: BAE38539.1
BC038608 mRNA Translation: AAH38608.1
CCDSiCCDS26975.1 [Q60969-1]
PIRiI49364
I49365
RefSeqiNP_062611.2, NM_019637.3 [Q60969-1]
UniGeneiMm.202561

Genome annotation databases

EnsembliENSMUST00000096420; ENSMUSP00000094153; ENSMUSG00000071748 [Q60969-1]
ENSMUST00000226873; ENSMUSP00000153854; ENSMUSG00000053205 [Q60969-1]
ENSMUST00000228311; ENSMUSP00000154148; ENSMUSG00000053205 [Q60969-1]
GeneIDi56291
KEGGimmu:56291
UCSCiuc007tgo.1 mouse [Q60969-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSTYX_MOUSE
AccessioniPrimary (citable) accession number: Q60969
Secondary accession number(s): Q3TMA3, Q60970, Q9DCF8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: February 28, 2003
Last modified: May 23, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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