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Protein

Serine/threonine/tyrosine-interacting protein

Gene

Styx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytically inactive phosphatase. Acts as a nuclear anchor for MAPK1/MAPK3 (ERK1/ERK2). Modulates cell-fate decisions and cell migration by spatiotemporal regulation of MAPK1/MAPK3 (ERK1/ERK2) (By similarity). Seems to play a role in spermiogenesis.By similarity

GO - Molecular functioni

GO - Biological processi

  • MAPK export from nucleus Source: UniProtKB
  • regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine/tyrosine-interacting protein
Alternative name(s):
Phosphoserine/threonine/tyrosine interaction protein
Protein tyrosine phosphatase-like protein
Gene namesi
Name:Styx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componentsi: Chromosome 14, Chromosome X

Organism-specific databases

MGIiMGI:1891150. Styx.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi120 – 1201G → C: Confers phosphatase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Serine/threonine/tyrosine-interacting proteinPRO_0000094951Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei184 – 1841PhosphoserineBy similarity
Modified residuei201 – 2011PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ60969.
PaxDbiQ60969.
PRIDEiQ60969.

PTM databases

iPTMnetiQ60969.
PhosphoSiteiQ60969.

Expressioni

Tissue specificityi

Widely expressed with highest levels in muscle, testis and brain.

Gene expression databases

BgeeiENSMUSG00000053205.
GenevisibleiQ60969. MM.

Interactioni

Subunit structurei

Interacts with MAPK1; independently of MAPK1 phosphorylation status. Interacts with CARHSP1/Crhsp-24.By similarity

Protein-protein interaction databases

BioGridi207879. 1 interaction.
STRINGi10090.ENSMUSP00000107466.

Structurei

3D structure databases

ProteinModelPortaliQ60969.
SMRiQ60969. Positions 26-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini96 – 16772Tyrosine-protein phosphataseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118853.
HOGENOMiHOG000090240.
HOVERGENiHBG044540.
InParanoidiQ60969.
KOiK18042.
OMAiWAYPMRR.
OrthoDBiEOG091G0249.
PhylomeDBiQ60969.
TreeFamiTF350439.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q60969-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDVKLEFPS LPQCKDDAEE WTYPMRREMQ EVLPGLFLGP YSSAMKSKLP
60 70 80 90 100
ILQKHGITHI ICIRQNIEAN FIKPNFQQLF RYLVLDIADN PVENIIRFFP
110 120 130 140 150
MTKEFIDGSL QNGGKVLVHG NAGISRSAAF VIAYIMETFG MKYRDAFAYV
160 170 180 190 200
QERRFCINPN AGFVHQLQEY EAIYLAKLTI QMMSPLQIER SLAVHSGTTG
210 220
SVKRTHEEDD DFGNMQVATA QNG
Length:223
Mass (Da):25,430
Last modified:February 28, 2003 - v2
Checksum:i7D13AFEF2B66B723
GO
Isoform 2 (identifier: Q60969-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     169-223: EYEAIYLAKL...NMQVATAQNG → LWLSWNSARS...TSKNKQTNNS

Show »
Length:204
Mass (Da):23,550
Checksum:i59F79FF3E66EC8F7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111L → V in AAA87036 (PubMed:7592916).Curated
Sequence conflicti11 – 111L → V in AAA87037 (PubMed:7592916).Curated
Sequence conflicti81 – 811R → T in BAC32931 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei169 – 22355EYEAI…TAQNG → LWLSWNSARSAPLPLKQRQV YHCAFKTSKNKQTNNS in isoform 2. 1 PublicationVSP_005174Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34973 mRNA. Translation: AAA87036.1.
U34973 mRNA. Translation: AAA87037.1.
AK002822 mRNA. Translation: BAB22384.1.
AK030122 mRNA. Translation: BAC26793.1.
AK046970 mRNA. Translation: BAC32931.1.
AK166043 mRNA. Translation: BAE38539.1.
BC038608 mRNA. Translation: AAH38608.1.
CCDSiCCDS26975.1. [Q60969-1]
PIRiI49364.
I49365.
RefSeqiNP_062611.2. NM_019637.3. [Q60969-1]
UniGeneiMm.202561.

Genome annotation databases

EnsembliENSMUST00000096420; ENSMUSP00000094153; ENSMUSG00000071748. [Q60969-1]
ENSMUST00000111835; ENSMUSP00000107466; ENSMUSG00000053205. [Q60969-1]
GeneIDi56291.
KEGGimmu:56291.
UCSCiuc007tgo.1. mouse. [Q60969-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34973 mRNA. Translation: AAA87036.1.
U34973 mRNA. Translation: AAA87037.1.
AK002822 mRNA. Translation: BAB22384.1.
AK030122 mRNA. Translation: BAC26793.1.
AK046970 mRNA. Translation: BAC32931.1.
AK166043 mRNA. Translation: BAE38539.1.
BC038608 mRNA. Translation: AAH38608.1.
CCDSiCCDS26975.1. [Q60969-1]
PIRiI49364.
I49365.
RefSeqiNP_062611.2. NM_019637.3. [Q60969-1]
UniGeneiMm.202561.

3D structure databases

ProteinModelPortaliQ60969.
SMRiQ60969. Positions 26-176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207879. 1 interaction.
STRINGi10090.ENSMUSP00000107466.

PTM databases

iPTMnetiQ60969.
PhosphoSiteiQ60969.

Proteomic databases

MaxQBiQ60969.
PaxDbiQ60969.
PRIDEiQ60969.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000096420; ENSMUSP00000094153; ENSMUSG00000071748. [Q60969-1]
ENSMUST00000111835; ENSMUSP00000107466; ENSMUSG00000053205. [Q60969-1]
GeneIDi56291.
KEGGimmu:56291.
UCSCiuc007tgo.1. mouse. [Q60969-1]

Organism-specific databases

CTDi6815.
MGIiMGI:1891150. Styx.

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118853.
HOGENOMiHOG000090240.
HOVERGENiHBG044540.
InParanoidiQ60969.
KOiK18042.
OMAiWAYPMRR.
OrthoDBiEOG091G0249.
PhylomeDBiQ60969.
TreeFamiTF350439.

Miscellaneous databases

PROiQ60969.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053205.
GenevisibleiQ60969. MM.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTYX_MOUSE
AccessioniPrimary (citable) accession number: Q60969
Secondary accession number(s): Q3TMA3, Q60970, Q9DCF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: February 28, 2003
Last modified: September 7, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Contains a Gly residue instead of a conserved Cys residue in the dsPTPase catalytic loop which renders it catalytically inactive as a phosphatase. The binding pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases.
STYX deficient male mice show, despite a normal mating behavior, a disrupted spermatid development, resulting in a >1000-fold decrease in spermatozoa production.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.