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Protein

Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1

Gene

Papss1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway (PubMed:7493984). Required for normal biosynthesis of sulfated L-selectin ligands in endothelial cells (By similarity).By similarity1 Publication

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.1 Publication
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.1 Publication

Pathwayi: sulfate assimilation

This protein is involved in the pathway sulfate assimilation, which is part of Sulfur metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway sulfate assimilation and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101Adenylyl sulfateBy similarity1
Binding sitei171Adenylyl sulfateBy similarity1
Binding sitei207ATP 1; via carbonyl oxygenBy similarity1
Binding sitei212ATP 1By similarity1
Binding sitei563ATP 2; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 67ATP 1By similarity6
Nucleotide bindingi419 – 422ATP 2By similarity4
Nucleotide bindingi521 – 525ATP 2By similarity5

GO - Molecular functioni

  • adenylylsulfate kinase activity Source: MGI
  • ATP binding Source: UniProtKB-KW
  • nucleotidyltransferase activity Source: UniProtKB
  • protein homodimerization activity Source: MGI
  • sulfate adenylyltransferase (ATP) activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionKinase, Nucleotidyltransferase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-174362. Transport and synthesis of PAPS.
UniPathwayiUPA00097.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Short name:
PAPS synthase 1
Short name:
PAPSS 1
Alternative name(s):
Sulfurylase kinase 1
Short name:
SK 1
Short name:
SK1
Including the following 2 domains:
Sulfate adenylyltransferase (EC:2.7.7.41 Publication)
Alternative name(s):
ATP-sulfurylase
Sulfate adenylate transferase
Short name:
SAT
Adenylyl-sulfate kinase (EC:2.7.1.251 Publication)
Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate synthase
APS kinase
Adenosine-5'-phosphosulfate 3'-phosphotransferase
Adenylylsulfate 3'-phosphotransferase
Gene namesi
Name:Papss1
Synonyms:Asapk, Atpsk1, Papss
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1330587. Papss1.

Subcellular locationi

GO - Cellular componenti

  • sulfate adenylyltransferase complex (ATP) Source: MGI

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001059601 – 624Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1Add BLAST624

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei12N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ60967.
MaxQBiQ60967.
PaxDbiQ60967.
PRIDEiQ60967.

PTM databases

iPTMnetiQ60967.
PhosphoSitePlusiQ60967.

Expressioni

Tissue specificityi

Expressed in the neonatal brain and in cartilage.1 Publication

Gene expression databases

BgeeiENSMUSG00000028032.
CleanExiMM_PAPSS1.
ExpressionAtlasiQ60967. baseline and differential.
GenevisibleiQ60967. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204832. 26 interactors.
IntActiQ60967. 29 interactors.
MINTiMINT-4106569.
STRINGi10090.ENSMUSP00000029666.

Structurei

3D structure databases

ProteinModelPortaliQ60967.
SMRiQ60967.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 225Adenylyl-sulfate kinaseBy similarityAdd BLAST225
Regioni89 – 92Adenylyl sulfate bindingBy similarity4
Regioni106 – 109Adenylyl sulfate bindingBy similarity4
Regioni132 – 133Adenylyl sulfate bindingBy similarity2
Regioni184 – 185Adenylyl sulfate bindingBy similarity2
Regioni234 – 624Sulfate adenylyltransferaseBy similarityAdd BLAST391

Sequence similaritiesi

In the N-terminal section; belongs to the APS kinase family.Curated
In the C-terminal section; belongs to the sulfate adenylyltransferase family.Curated

Phylogenomic databases

eggNOGiKOG0635. Eukaryota.
KOG4238. Eukaryota.
COG0529. LUCA.
COG2046. LUCA.
GeneTreeiENSGT00390000009613.
HOGENOMiHOG000069045.
HOVERGENiHBG053503.
InParanoidiQ60967.
KOiK13811.
OMAiCKEHPYI.
OrthoDBiEOG091G07ZR.
PhylomeDBiQ60967.
TreeFamiTF313143.

Family and domain databases

CDDicd02027. APSK. 1 hit.
cd00517. ATPS. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase. 1 hit.
InterProiView protein in InterPro
IPR002891. APS_kinase.
IPR025980. ATP-Sase_PUA-like_dom.
IPR027417. P-loop_NTPase.
IPR015947. PUA-like_domain.
IPR014729. Rossmann-like_a/b/a_fold.
IPR024951. Sulfurylase_cat_dom.
IPR002650. Sulphate_adenylyltransferase.
PfamiView protein in Pfam
PF01747. ATP-sulfurylase. 1 hit.
PF14306. PUA_2. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.
TIGR00339. sopT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q60967-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIPGSLCKK VKLSNNAQNW GMQRATNVTY QAHHVSRNKR GQVVGTRGGF
60 70 80 90 100
RGCTVWLTGL SGAGKTTVSM ALEEYLVCHG IPCYTLDGDN IRQGLNKNLG
110 120 130 140 150
FSPEDREENV RRIAEVAKLF ADAGLVCITS FISPYTQDRN NARQIHEGAS
160 170 180 190 200
LPFFEVFVDA PLHVCEQRDV KGLYKKARAG EIKGFTGIDS EYEKPEAPEL
210 220 230 240 250
VLKTDSCDVN DCVQQVVELL QERDIVPVDA SYEVKELYVP ENKLHLAKTD
260 270 280 290 300
AEALPALKIN KVDMQWVQVL AEGWATPLNG FMREREYLQC LHFDCLLDGG
310 320 330 340 350
VINLSVPIVL TATHEDKERL DGCTAFALVY EGRRVAILRN PEFFEHRKEE
360 370 380 390 400
RCARQWGTTC KNHPYIKMVL EQGDWLIGGD LQVLDRIYWN DGLDQYRLTP
410 420 430 440 450
TELKQKFKDM NADAVFAFQL RNPVHNGHAL LMQDTHKQLL ERGYRRPVLL
460 470 480 490 500
LHPLGGWTKD DDVPLMWRMK QHAAVLEEGI LDPETTVVAI FPSPMMYAGP
510 520 530 540 550
TEVQWHCRAR MVAGANFYIV GRDPAGMPHP ETGKDLYEPT HGAKVLTMAP
560 570 580 590 600
GLITLEIVPF RVAAYNKKKK RMDYYDSEHH EDFEFISGTR MRKLAREGQK
610 620
PPEGFMAPKA WTVLVEYYKS LEKA
Length:624
Mass (Da):70,794
Last modified:November 1, 1996 - v1
Checksum:iB487EFAF9B78BE3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34883 mRNA. Translation: AAC52328.1.
AK146507 mRNA. Translation: BAE27221.1.
AK153296 mRNA. Translation: BAE31878.1.
AK168982 mRNA. Translation: BAE40782.1.
AK169124 mRNA. Translation: BAE40904.1.
CH466532 Genomic DNA. Translation: EDL12198.1.
CCDSiCCDS38641.1.
RefSeqiNP_035993.1. NM_011863.2.
UniGeneiMm.244912.

Genome annotation databases

EnsembliENSMUST00000029666; ENSMUSP00000029666; ENSMUSG00000028032.
GeneIDi23971.
KEGGimmu:23971.
UCSCiuc008rjr.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPAPS1_MOUSE
AccessioniPrimary (citable) accession number: Q60967
Secondary accession number(s): Q3U647
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: June 7, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families