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Protein

Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1

Gene

Papss1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.

Pathwayi: sulfate assimilation

This protein is involved in the pathway sulfate assimilation, which is part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the pathway sulfate assimilation and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei133 – 1331Phosphoserine intermediateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi59 – 668ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-174362. Transport and synthesis of PAPS.
UniPathwayiUPA00097.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Short name:
PAPS synthase 1
Short name:
PAPSS 1
Alternative name(s):
Sulfurylase kinase 1
Short name:
SK 1
Short name:
SK1
Including the following 2 domains:
Sulfate adenylyltransferase (EC:2.7.7.4)
Alternative name(s):
ATP-sulfurylase
Sulfate adenylate transferase
Short name:
SAT
Adenylyl-sulfate kinase (EC:2.7.1.25)
Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate synthase
APS kinase
Adenosine-5'-phosphosulfate 3'-phosphotransferase
Adenylylsulfate 3'-phosphotransferase
Gene namesi
Name:Papss1
Synonyms:Asapk, Atpsk1, Papss
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1330587. Papss1.

Subcellular locationi

GO - Cellular componenti

  • sulfate adenylyltransferase complex (ATP) Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 624624Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1PRO_0000105960Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei12 – 121N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ60967.
MaxQBiQ60967.
PaxDbiQ60967.
PRIDEiQ60967.

PTM databases

iPTMnetiQ60967.
PhosphoSiteiQ60967.

Expressioni

Tissue specificityi

Expressed in the neonatal brain and in cartilage.

Gene expression databases

BgeeiQ60967.
CleanExiMM_PAPSS1.
ExpressionAtlasiQ60967. baseline and differential.
GenevisibleiQ60967. MM.

Interactioni

Protein-protein interaction databases

BioGridi204832. 26 interactions.
IntActiQ60967. 29 interactions.
MINTiMINT-4106569.
STRINGi10090.ENSMUSP00000029666.

Structurei

3D structure databases

ProteinModelPortaliQ60967.
SMRiQ60967. Positions 25-623.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – ?220220Adenylyl-sulfate kinaseAdd
BLAST
Regioni?221 – 624404Sulfate adenylyltransferaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi521 – 5255PP-motif

Sequence similaritiesi

In the N-terminal section; belongs to the APS kinase family.Curated
In the C-terminal section; belongs to the sulfate adenylyltransferase family.Curated

Phylogenomic databases

eggNOGiKOG0635. Eukaryota.
KOG4238. Eukaryota.
COG0529. LUCA.
COG2046. LUCA.
GeneTreeiENSGT00390000009613.
HOGENOMiHOG000069045.
HOVERGENiHBG053503.
InParanoidiQ60967.
KOiK13811.
OMAiQKTCLQV.
OrthoDBiEOG74TWZ4.
PhylomeDBiQ60967.
TreeFamiTF313143.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase.
InterProiIPR002891. APS_kinase.
IPR025980. ATP-Sase_PUA-like_dom.
IPR027417. P-loop_NTPase.
IPR015947. PUA-like_domain.
IPR014729. Rossmann-like_a/b/a_fold.
IPR024951. Sulfurylase_cat_dom.
IPR002650. Sulphate_adenylyltransferase.
[Graphical view]
PfamiPF01747. ATP-sulfurylase. 1 hit.
PF14306. PUA_2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.
TIGR00339. sopT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q60967-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIPGSLCKK VKLSNNAQNW GMQRATNVTY QAHHVSRNKR GQVVGTRGGF
60 70 80 90 100
RGCTVWLTGL SGAGKTTVSM ALEEYLVCHG IPCYTLDGDN IRQGLNKNLG
110 120 130 140 150
FSPEDREENV RRIAEVAKLF ADAGLVCITS FISPYTQDRN NARQIHEGAS
160 170 180 190 200
LPFFEVFVDA PLHVCEQRDV KGLYKKARAG EIKGFTGIDS EYEKPEAPEL
210 220 230 240 250
VLKTDSCDVN DCVQQVVELL QERDIVPVDA SYEVKELYVP ENKLHLAKTD
260 270 280 290 300
AEALPALKIN KVDMQWVQVL AEGWATPLNG FMREREYLQC LHFDCLLDGG
310 320 330 340 350
VINLSVPIVL TATHEDKERL DGCTAFALVY EGRRVAILRN PEFFEHRKEE
360 370 380 390 400
RCARQWGTTC KNHPYIKMVL EQGDWLIGGD LQVLDRIYWN DGLDQYRLTP
410 420 430 440 450
TELKQKFKDM NADAVFAFQL RNPVHNGHAL LMQDTHKQLL ERGYRRPVLL
460 470 480 490 500
LHPLGGWTKD DDVPLMWRMK QHAAVLEEGI LDPETTVVAI FPSPMMYAGP
510 520 530 540 550
TEVQWHCRAR MVAGANFYIV GRDPAGMPHP ETGKDLYEPT HGAKVLTMAP
560 570 580 590 600
GLITLEIVPF RVAAYNKKKK RMDYYDSEHH EDFEFISGTR MRKLAREGQK
610 620
PPEGFMAPKA WTVLVEYYKS LEKA
Length:624
Mass (Da):70,794
Last modified:November 1, 1996 - v1
Checksum:iB487EFAF9B78BE3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34883 mRNA. Translation: AAC52328.1.
CCDSiCCDS38641.1.
RefSeqiNP_035993.1. NM_011863.2.
UniGeneiMm.244912.

Genome annotation databases

EnsembliENSMUST00000029666; ENSMUSP00000029666; ENSMUSG00000028032.
GeneIDi23971.
KEGGimmu:23971.
UCSCiuc008rjr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34883 mRNA. Translation: AAC52328.1.
CCDSiCCDS38641.1.
RefSeqiNP_035993.1. NM_011863.2.
UniGeneiMm.244912.

3D structure databases

ProteinModelPortaliQ60967.
SMRiQ60967. Positions 25-623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204832. 26 interactions.
IntActiQ60967. 29 interactions.
MINTiMINT-4106569.
STRINGi10090.ENSMUSP00000029666.

PTM databases

iPTMnetiQ60967.
PhosphoSiteiQ60967.

Proteomic databases

EPDiQ60967.
MaxQBiQ60967.
PaxDbiQ60967.
PRIDEiQ60967.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029666; ENSMUSP00000029666; ENSMUSG00000028032.
GeneIDi23971.
KEGGimmu:23971.
UCSCiuc008rjr.2. mouse.

Organism-specific databases

CTDi9061.
MGIiMGI:1330587. Papss1.

Phylogenomic databases

eggNOGiKOG0635. Eukaryota.
KOG4238. Eukaryota.
COG0529. LUCA.
COG2046. LUCA.
GeneTreeiENSGT00390000009613.
HOGENOMiHOG000069045.
HOVERGENiHBG053503.
InParanoidiQ60967.
KOiK13811.
OMAiQKTCLQV.
OrthoDBiEOG74TWZ4.
PhylomeDBiQ60967.
TreeFamiTF313143.

Enzyme and pathway databases

UniPathwayiUPA00097.
ReactomeiR-MMU-174362. Transport and synthesis of PAPS.

Miscellaneous databases

NextBioi303857.
PROiQ60967.
SOURCEiSearch...

Gene expression databases

BgeeiQ60967.
CleanExiMM_PAPSS1.
ExpressionAtlasiQ60967. baseline and differential.
GenevisibleiQ60967. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase.
InterProiIPR002891. APS_kinase.
IPR025980. ATP-Sase_PUA-like_dom.
IPR027417. P-loop_NTPase.
IPR015947. PUA-like_domain.
IPR014729. Rossmann-like_a/b/a_fold.
IPR024951. Sulfurylase_cat_dom.
IPR002650. Sulphate_adenylyltransferase.
[Graphical view]
PfamiPF01747. ATP-sulfurylase. 1 hit.
PF14306. PUA_2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.
TIGR00339. sopT. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The isolation and characterization of cDNA encoding the mouse bifunctional ATP sulfurylase-adenosine 5'-phosphosulfate kinase."
    Li H., Deyrup A., Mensch J.R. Jr., Domowicz M., Konstantinidis A.K., Schwartz N.B.
    J. Biol. Chem. 270:29453-29459(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-12, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiPAPS1_MOUSE
AccessioniPrimary (citable) accession number: Q60967
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.