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Q60963 (PAFA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Platelet-activating factor acetylhydrolase

Short name=PAF acetylhydrolase
EC=3.1.1.47
Alternative name(s):
1-alkyl-2-acetylglycerophosphocholine esterase
2-acetyl-1-alkylglycerophosphocholine esterase
LDL-associated phospholipase A2
Short name=LDL-PLA(2)
PAF 2-acylhydrolase
Gene names
Name:Pla2g7
Synonyms:Pafah
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

Catalytic activity

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Subcellular location

Secretedextracellular space.

Tissue specificity

Plasma.

Sequence similarities

Belongs to the AB hydrolase superfamily. Lipase family.

Sequence caution

The sequence AAC52274.1 differs from that shown. Reason: Frameshift at positions 342 and 349.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 By similarity
Chain22 – 440419Platelet-activating factor acetylhydrolase
PRO_0000017834

Sites

Active site2721Nucleophile By similarity
Active site2951Charge relay system By similarity
Active site3501Charge relay system By similarity

Amino acid modifications

Glycosylation591N-linked (GlcNAc...) Potential
Glycosylation751N-linked (GlcNAc...) Potential
Glycosylation1991N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict4271V → A in EDL23404. Ref.3
Sequence conflict4271V → A in EDL23406. Ref.3
Sequence conflict4271V → A in AAH10726. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q60963 [UniParc].

Last modified November 2, 2010. Version 2.
Checksum: 17C8B4D28F1ADC94

FASTA44049,258
        10         20         30         40         50         60 
MVPLKLQALF CLLCCLPWVH PFHWQDTSSF DFRPSVMFHK LQSVMSAAGS GHSKIPKGNG 

        70         80         90        100        110        120 
SYPVGCTDLM FGYGNESVFV RLYYPAQDQG RLDTVWIPNK EYFLGLSIFL GTPSIVGNIL 

       130        140        150        160        170        180 
HLLYGSLTTP ASWNSPLRTG EKYPLIVFSH GLGAFRTIYS AIGIGLASNG FIVATVEHRD 

       190        200        210        220        230        240 
RSASATYFFE DQVAAKVENR SWLYLRKVKQ EESESVRKEQ VQQRAIECSR ALSAILDIEH 

       250        260        270        280        290        300 
GDPKENVLGS AFDMKQLKDA IDETKIALMG HSFGGATVLQ ALSEDQRFRC GVALDPWMYP 

       310        320        330        340        350        360 
VNEELYSRTL QPLLFINSAK FQTPKDIAKM KKFYQPDKER KMITIKGSVH QNFDDFTFVT 

       370        380        390        400        410        420 
GKIIGNKLTL KGEIDSRVAI DLTNKASMAF LQKHLGLQKD FDQWDPLVEG DDENLIPGSP 

       430        440 
FDAVTQVPAQ QHSPGSQTQN 

« Hide

References

« Hide 'large scale' references
[1]"Plasma platelet-activating factor acetylhydrolase is a secreted phospholipase A2 with a catalytic triad."
Tjoelker L.W., Eberhardt C., Unger J., le Trong H., Zimmerman G.A., McIntyre T.M., Stafforini D.M., Prescott S.M., Gray P.W.
J. Biol. Chem. 270:25481-25487(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Spleen.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Spinal ganglion.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U34277 mRNA. Translation: AAC52274.1. Frameshift.
AK051454 mRNA. Translation: BAC34647.1.
CT010585 Genomic DNA. Translation: CAQ11111.1.
CH466559 Genomic DNA. Translation: EDL23404.1.
CH466559 Genomic DNA. Translation: EDL23406.1.
BC010726 mRNA. Translation: AAH10726.1.
CCDSCCDS28796.1.
RefSeqNP_038765.2. NM_013737.5.
XP_006524428.1. XM_006524365.1.
XP_006524429.1. XM_006524366.1.
UniGeneMm.9277.

3D structure databases

ProteinModelPortalQ60963.
SMRQ60963. Positions 53-419.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ60963. 1 interaction.
STRING10090.ENSMUSP00000024706.

Chemistry

ChEMBLCHEMBL5383.

Proteomic databases

MaxQBQ60963.
PaxDbQ60963.
PRIDEQ60963.

Protocols and materials databases

DNASU27226.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000024706; ENSMUSP00000024706; ENSMUSG00000023913.
GeneID27226.
KEGGmmu:27226.
UCSCuc008cpd.1. mouse.

Organism-specific databases

CTD7941.
MGIMGI:1351327. Pla2g7.

Phylogenomic databases

eggNOGCOG4188.
GeneTreeENSGT00390000005233.
HOGENOMHOG000008053.
HOVERGENHBG001322.
InParanoidQ8BKM3.
KOK01062.
OMAILFINTH.
PhylomeDBQ60963.
TreeFamTF313831.

Gene expression databases

ArrayExpressQ60963.
BgeeQ60963.
GenevestigatorQ60963.

Family and domain databases

Gene3D3.40.50.1820. 3 hits.
InterProIPR029058. AB_hydrolase.
IPR005065. PAF_acetylhydro.
IPR016715. PAF_acetylhydro_eukaryote.
[Graphical view]
PANTHERPTHR10272. PTHR10272. 1 hit.
PfamPF03403. PAF-AH_p_II. 1 hit.
[Graphical view]
PIRSFPIRSF018169. PAF_acetylhydrolase. 1 hit.
SUPFAMSSF53474. SSF53474. 3 hits.
PROSITEPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio305148.
PROQ60963.
SOURCESearch...

Entry information

Entry namePAFA_MOUSE
AccessionPrimary (citable) accession number: Q60963
Secondary accession number(s): Q8BKM3, Q921T4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 2, 2010
Last modified: July 9, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot