Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q60963

- PAFA_MOUSE

UniProt

Q60963 - PAFA_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Platelet-activating factor acetylhydrolase

Gene

Pla2g7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

Catalytic activityi

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei272 – 2721NucleophileBy similarity
Active sitei295 – 2951Charge relay systemPROSITE-ProRule annotation
Active sitei350 – 3501Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

  1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC
  2. calcium-independent phospholipase A2 activity Source: Ensembl
  3. phospholipid binding Source: Ensembl

GO - Biological processi

  1. inflammatory response Source: MGI
  2. lipid catabolic process Source: UniProtKB-KW
  3. lipid oxidation Source: Ensembl
  4. low-density lipoprotein particle remodeling Source: Ensembl
  5. plasma lipoprotein particle oxidation Source: Ensembl
  6. positive regulation of monocyte chemotaxis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-activating factor acetylhydrolase (EC:3.1.1.47)
Short name:
PAF acetylhydrolase
Alternative name(s):
1-alkyl-2-acetylglycerophosphocholine esterase
2-acetyl-1-alkylglycerophosphocholine esterase
LDL-associated phospholipase A2
Short name:
LDL-PLA(2)
PAF 2-acylhydrolase
Gene namesi
Name:Pla2g7
Synonyms:Pafah
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1351327. Pla2g7.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. low-density lipoprotein particle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121By similarityAdd
BLAST
Chaini22 – 440419Platelet-activating factor acetylhydrolasePRO_0000017834Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi59 – 591N-linked (GlcNAc...)Sequence Analysis
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi199 – 1991N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ60963.
PaxDbiQ60963.
PRIDEiQ60963.

Expressioni

Tissue specificityi

Plasma.

Gene expression databases

BgeeiQ60963.
ExpressionAtlasiQ60963. baseline and differential.
GenevestigatoriQ60963.

Interactioni

Protein-protein interaction databases

IntActiQ60963. 1 interaction.
STRINGi10090.ENSMUSP00000024706.

Structurei

3D structure databases

ProteinModelPortaliQ60963.
SMRiQ60963. Positions 53-419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4188.
GeneTreeiENSGT00390000005233.
HOGENOMiHOG000008053.
HOVERGENiHBG001322.
InParanoidiQ60963.
KOiK01062.
OMAiILFINTH.
PhylomeDBiQ60963.
TreeFamiTF313831.

Family and domain databases

Gene3Di3.40.50.1820. 3 hits.
InterProiIPR029058. AB_hydrolase.
IPR005065. PAF_acetylhydro.
IPR016715. PAF_acetylhydro_eukaryote.
[Graphical view]
PANTHERiPTHR10272. PTHR10272. 1 hit.
PfamiPF03403. PAF-AH_p_II. 1 hit.
[Graphical view]
PIRSFiPIRSF018169. PAF_acetylhydrolase. 1 hit.
SUPFAMiSSF53474. SSF53474. 3 hits.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q60963-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVPLKLQALF CLLCCLPWVH PFHWQDTSSF DFRPSVMFHK LQSVMSAAGS
60 70 80 90 100
GHSKIPKGNG SYPVGCTDLM FGYGNESVFV RLYYPAQDQG RLDTVWIPNK
110 120 130 140 150
EYFLGLSIFL GTPSIVGNIL HLLYGSLTTP ASWNSPLRTG EKYPLIVFSH
160 170 180 190 200
GLGAFRTIYS AIGIGLASNG FIVATVEHRD RSASATYFFE DQVAAKVENR
210 220 230 240 250
SWLYLRKVKQ EESESVRKEQ VQQRAIECSR ALSAILDIEH GDPKENVLGS
260 270 280 290 300
AFDMKQLKDA IDETKIALMG HSFGGATVLQ ALSEDQRFRC GVALDPWMYP
310 320 330 340 350
VNEELYSRTL QPLLFINSAK FQTPKDIAKM KKFYQPDKER KMITIKGSVH
360 370 380 390 400
QNFDDFTFVT GKIIGNKLTL KGEIDSRVAI DLTNKASMAF LQKHLGLQKD
410 420 430 440
FDQWDPLVEG DDENLIPGSP FDAVTQVPAQ QHSPGSQTQN
Length:440
Mass (Da):49,258
Last modified:November 2, 2010 - v2
Checksum:i17C8B4D28F1ADC94
GO

Sequence cautioni

The sequence AAC52274.1 differs from that shown. Reason: Frameshift at positions 342 and 349.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti427 – 4271V → A in EDL23404. (PubMed:19468303)Curated
Sequence conflicti427 – 4271V → A in EDL23406. (PubMed:19468303)Curated
Sequence conflicti427 – 4271V → A in AAH10726. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U34277 mRNA. Translation: AAC52274.1. Frameshift.
AK051454 mRNA. Translation: BAC34647.1.
CT010585 Genomic DNA. Translation: CAQ11111.1.
CH466559 Genomic DNA. Translation: EDL23404.1.
CH466559 Genomic DNA. Translation: EDL23406.1.
BC010726 mRNA. Translation: AAH10726.1.
CCDSiCCDS28796.1.
RefSeqiNP_038765.2. NM_013737.5.
XP_006524428.1. XM_006524365.1.
XP_006524429.1. XM_006524366.1.
UniGeneiMm.9277.

Genome annotation databases

EnsembliENSMUST00000024706; ENSMUSP00000024706; ENSMUSG00000023913.
GeneIDi27226.
KEGGimmu:27226.
UCSCiuc008cpd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U34277 mRNA. Translation: AAC52274.1 . Frameshift.
AK051454 mRNA. Translation: BAC34647.1 .
CT010585 Genomic DNA. Translation: CAQ11111.1 .
CH466559 Genomic DNA. Translation: EDL23404.1 .
CH466559 Genomic DNA. Translation: EDL23406.1 .
BC010726 mRNA. Translation: AAH10726.1 .
CCDSi CCDS28796.1.
RefSeqi NP_038765.2. NM_013737.5.
XP_006524428.1. XM_006524365.1.
XP_006524429.1. XM_006524366.1.
UniGenei Mm.9277.

3D structure databases

ProteinModelPortali Q60963.
SMRi Q60963. Positions 53-419.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q60963. 1 interaction.
STRINGi 10090.ENSMUSP00000024706.

Chemistry

ChEMBLi CHEMBL5383.

Proteomic databases

MaxQBi Q60963.
PaxDbi Q60963.
PRIDEi Q60963.

Protocols and materials databases

DNASUi 27226.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000024706 ; ENSMUSP00000024706 ; ENSMUSG00000023913 .
GeneIDi 27226.
KEGGi mmu:27226.
UCSCi uc008cpd.1. mouse.

Organism-specific databases

CTDi 7941.
MGIi MGI:1351327. Pla2g7.

Phylogenomic databases

eggNOGi COG4188.
GeneTreei ENSGT00390000005233.
HOGENOMi HOG000008053.
HOVERGENi HBG001322.
InParanoidi Q60963.
KOi K01062.
OMAi ILFINTH.
PhylomeDBi Q60963.
TreeFami TF313831.

Miscellaneous databases

NextBioi 305148.
PROi Q60963.
SOURCEi Search...

Gene expression databases

Bgeei Q60963.
ExpressionAtlasi Q60963. baseline and differential.
Genevestigatori Q60963.

Family and domain databases

Gene3Di 3.40.50.1820. 3 hits.
InterProi IPR029058. AB_hydrolase.
IPR005065. PAF_acetylhydro.
IPR016715. PAF_acetylhydro_eukaryote.
[Graphical view ]
PANTHERi PTHR10272. PTHR10272. 1 hit.
Pfami PF03403. PAF-AH_p_II. 1 hit.
[Graphical view ]
PIRSFi PIRSF018169. PAF_acetylhydrolase. 1 hit.
SUPFAMi SSF53474. SSF53474. 3 hits.
PROSITEi PS00120. LIPASE_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Plasma platelet-activating factor acetylhydrolase is a secreted phospholipase A2 with a catalytic triad."
    Tjoelker L.W., Eberhardt C., Unger J., le Trong H., Zimmerman G.A., McIntyre T.M., Stafforini D.M., Prescott S.M., Gray P.W.
    J. Biol. Chem. 270:25481-25487(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Spinal ganglion.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiPAFA_MOUSE
AccessioniPrimary (citable) accession number: Q60963
Secondary accession number(s): Q8BKM3, Q921T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 2, 2010
Last modified: October 29, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3