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Q60963

- PAFA_MOUSE

UniProt

Q60963 - PAFA_MOUSE

Protein

Platelet-activating factor acetylhydrolase

Gene

Pla2g7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (02 Nov 2010)
      Previous versions | rss
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    Functioni

    Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

    Catalytic activityi

    1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei272 – 2721NucleophileBy similarity
    Active sitei295 – 2951Charge relay systemPROSITE-ProRule annotation
    Active sitei350 – 3501Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC
    2. calcium-independent phospholipase A2 activity Source: Ensembl
    3. phospholipid binding Source: Ensembl

    GO - Biological processi

    1. inflammatory response Source: MGI
    2. lipid catabolic process Source: UniProtKB-KW
    3. lipid oxidation Source: Ensembl
    4. low-density lipoprotein particle remodeling Source: Ensembl
    5. plasma lipoprotein particle oxidation Source: Ensembl
    6. positive regulation of monocyte chemotaxis Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Platelet-activating factor acetylhydrolase (EC:3.1.1.47)
    Short name:
    PAF acetylhydrolase
    Alternative name(s):
    1-alkyl-2-acetylglycerophosphocholine esterase
    2-acetyl-1-alkylglycerophosphocholine esterase
    LDL-associated phospholipase A2
    Short name:
    LDL-PLA(2)
    PAF 2-acylhydrolase
    Gene namesi
    Name:Pla2g7
    Synonyms:Pafah
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1351327. Pla2g7.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. low-density lipoprotein particle Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121By similarityAdd
    BLAST
    Chaini22 – 440419Platelet-activating factor acetylhydrolasePRO_0000017834Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi59 – 591N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi199 – 1991N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ60963.
    PaxDbiQ60963.
    PRIDEiQ60963.

    Expressioni

    Tissue specificityi

    Plasma.

    Gene expression databases

    ArrayExpressiQ60963.
    BgeeiQ60963.
    GenevestigatoriQ60963.

    Interactioni

    Protein-protein interaction databases

    IntActiQ60963. 1 interaction.
    STRINGi10090.ENSMUSP00000024706.

    Structurei

    3D structure databases

    ProteinModelPortaliQ60963.
    SMRiQ60963. Positions 53-419.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily. Lipase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4188.
    GeneTreeiENSGT00390000005233.
    HOGENOMiHOG000008053.
    HOVERGENiHBG001322.
    InParanoidiQ8BKM3.
    KOiK01062.
    OMAiILFINTH.
    PhylomeDBiQ60963.
    TreeFamiTF313831.

    Family and domain databases

    Gene3Di3.40.50.1820. 3 hits.
    InterProiIPR029058. AB_hydrolase.
    IPR005065. PAF_acetylhydro.
    IPR016715. PAF_acetylhydro_eukaryote.
    [Graphical view]
    PANTHERiPTHR10272. PTHR10272. 1 hit.
    PfamiPF03403. PAF-AH_p_II. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018169. PAF_acetylhydrolase. 1 hit.
    SUPFAMiSSF53474. SSF53474. 3 hits.
    PROSITEiPS00120. LIPASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q60963-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVPLKLQALF CLLCCLPWVH PFHWQDTSSF DFRPSVMFHK LQSVMSAAGS    50
    GHSKIPKGNG SYPVGCTDLM FGYGNESVFV RLYYPAQDQG RLDTVWIPNK 100
    EYFLGLSIFL GTPSIVGNIL HLLYGSLTTP ASWNSPLRTG EKYPLIVFSH 150
    GLGAFRTIYS AIGIGLASNG FIVATVEHRD RSASATYFFE DQVAAKVENR 200
    SWLYLRKVKQ EESESVRKEQ VQQRAIECSR ALSAILDIEH GDPKENVLGS 250
    AFDMKQLKDA IDETKIALMG HSFGGATVLQ ALSEDQRFRC GVALDPWMYP 300
    VNEELYSRTL QPLLFINSAK FQTPKDIAKM KKFYQPDKER KMITIKGSVH 350
    QNFDDFTFVT GKIIGNKLTL KGEIDSRVAI DLTNKASMAF LQKHLGLQKD 400
    FDQWDPLVEG DDENLIPGSP FDAVTQVPAQ QHSPGSQTQN 440
    Length:440
    Mass (Da):49,258
    Last modified:November 2, 2010 - v2
    Checksum:i17C8B4D28F1ADC94
    GO

    Sequence cautioni

    The sequence AAC52274.1 differs from that shown. Reason: Frameshift at positions 342 and 349.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti427 – 4271V → A in EDL23404. (PubMed:19468303)Curated
    Sequence conflicti427 – 4271V → A in EDL23406. (PubMed:19468303)Curated
    Sequence conflicti427 – 4271V → A in AAH10726. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34277 mRNA. Translation: AAC52274.1. Frameshift.
    AK051454 mRNA. Translation: BAC34647.1.
    CT010585 Genomic DNA. Translation: CAQ11111.1.
    CH466559 Genomic DNA. Translation: EDL23404.1.
    CH466559 Genomic DNA. Translation: EDL23406.1.
    BC010726 mRNA. Translation: AAH10726.1.
    CCDSiCCDS28796.1.
    RefSeqiNP_038765.2. NM_013737.5.
    XP_006524428.1. XM_006524365.1.
    XP_006524429.1. XM_006524366.1.
    UniGeneiMm.9277.

    Genome annotation databases

    EnsembliENSMUST00000024706; ENSMUSP00000024706; ENSMUSG00000023913.
    GeneIDi27226.
    KEGGimmu:27226.
    UCSCiuc008cpd.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34277 mRNA. Translation: AAC52274.1 . Frameshift.
    AK051454 mRNA. Translation: BAC34647.1 .
    CT010585 Genomic DNA. Translation: CAQ11111.1 .
    CH466559 Genomic DNA. Translation: EDL23404.1 .
    CH466559 Genomic DNA. Translation: EDL23406.1 .
    BC010726 mRNA. Translation: AAH10726.1 .
    CCDSi CCDS28796.1.
    RefSeqi NP_038765.2. NM_013737.5.
    XP_006524428.1. XM_006524365.1.
    XP_006524429.1. XM_006524366.1.
    UniGenei Mm.9277.

    3D structure databases

    ProteinModelPortali Q60963.
    SMRi Q60963. Positions 53-419.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q60963. 1 interaction.
    STRINGi 10090.ENSMUSP00000024706.

    Chemistry

    ChEMBLi CHEMBL5383.

    Proteomic databases

    MaxQBi Q60963.
    PaxDbi Q60963.
    PRIDEi Q60963.

    Protocols and materials databases

    DNASUi 27226.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024706 ; ENSMUSP00000024706 ; ENSMUSG00000023913 .
    GeneIDi 27226.
    KEGGi mmu:27226.
    UCSCi uc008cpd.1. mouse.

    Organism-specific databases

    CTDi 7941.
    MGIi MGI:1351327. Pla2g7.

    Phylogenomic databases

    eggNOGi COG4188.
    GeneTreei ENSGT00390000005233.
    HOGENOMi HOG000008053.
    HOVERGENi HBG001322.
    InParanoidi Q8BKM3.
    KOi K01062.
    OMAi ILFINTH.
    PhylomeDBi Q60963.
    TreeFami TF313831.

    Miscellaneous databases

    NextBioi 305148.
    PROi Q60963.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q60963.
    Bgeei Q60963.
    Genevestigatori Q60963.

    Family and domain databases

    Gene3Di 3.40.50.1820. 3 hits.
    InterProi IPR029058. AB_hydrolase.
    IPR005065. PAF_acetylhydro.
    IPR016715. PAF_acetylhydro_eukaryote.
    [Graphical view ]
    PANTHERi PTHR10272. PTHR10272. 1 hit.
    Pfami PF03403. PAF-AH_p_II. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018169. PAF_acetylhydrolase. 1 hit.
    SUPFAMi SSF53474. SSF53474. 3 hits.
    PROSITEi PS00120. LIPASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Plasma platelet-activating factor acetylhydrolase is a secreted phospholipase A2 with a catalytic triad."
      Tjoelker L.W., Eberhardt C., Unger J., le Trong H., Zimmerman G.A., McIntyre T.M., Stafforini D.M., Prescott S.M., Gray P.W.
      J. Biol. Chem. 270:25481-25487(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Spleen.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Spinal ganglion.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiPAFA_MOUSE
    AccessioniPrimary (citable) accession number: Q60963
    Secondary accession number(s): Q8BKM3, Q921T4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 2, 2010
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3