Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Importin subunit alpha-5

Gene

Kpna1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-211227 Activation of DNA fragmentation factor

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit alpha-5
Alternative name(s):
Importin alpha-S1
Karyopherin subunit alpha-1
Nucleoprotein interactor 1
Short name:
NPI-1
RAG cohort protein 2
SRP1-beta
Gene namesi
Name:Kpna1
Synonyms:Rch2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:103560 Kpna1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207201 – 538Importin subunit alpha-5Add BLAST538

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei3PhosphothreonineBy similarity1
Modified residuei63PhosphoserineBy similarity1

Post-translational modificationi

Polyubiquitinated in the presence of RAG1 (in vitro).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ60960
MaxQBiQ60960
PaxDbiQ60960
PeptideAtlasiQ60960
PRIDEiQ60960

PTM databases

iPTMnetiQ60960
PhosphoSitePlusiQ60960

Expressioni

Tissue specificityi

Low levels in all tissues examined.

Gene expression databases

BgeeiENSMUSG00000022905
CleanExiMM_KPNA1
ExpressionAtlasiQ60960 baseline and differential
GenevisibleiQ60960 MM

Interactioni

Subunit structurei

Heterodimer; with KPNB1 (By similarity). Interacts with NSMF; the interaction occurs in a calcium-independent manner after synaptic NMDA receptor stimulation and is required for nuclear import of NSMF but is competed by CABP1 (By similarity). Interacts with APEX1 (via its N-terminus). Interacts with CTNNBL1 (via its N-terminal). Interacts with AICDA (via its NLS) (By similarity). Interacts with ANP32E and ZIC3. Interacts with SNAI1 (via zinc fingers) (By similarity). Interacts with DCAF8.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
SF1Q156373EBI-8573008,EBI-744603From Homo sapiens.

Protein-protein interaction databases

BioGridi201006, 6 interactors
DIPiDIP-48614N
IntActiQ60960, 4 interactors
MINTiQ60960
STRINGi10090.ENSMUSP00000004054

Structurei

3D structure databases

ProteinModelPortaliQ60960
SMRiQ60960
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 57IBBPROSITE-ProRule annotationAdd BLAST57
Repeati77 – 117ARM 1; truncatedAdd BLAST41
Repeati118 – 161ARM 2Add BLAST44
Repeati162 – 206ARM 3Add BLAST45
Repeati207 – 245ARM 4Add BLAST39
Repeati246 – 290ARM 5Add BLAST45
Repeati291 – 330ARM 6Add BLAST40
Repeati331 – 372ARM 7Add BLAST42
Repeati373 – 412ARM 8Add BLAST40
Repeati413 – 457ARM 9Add BLAST45
Repeati460 – 504ARM 10; atypicalAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni149 – 241NLS binding site (major)By similarityAdd BLAST93
Regioni245 – 437Binding to RAG1By similarityAdd BLAST193
Regioni318 – 406NLS binding site (minor)By similarityAdd BLAST89

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 51Nuclear localization signalBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi25 – 28Poly-Arg4

Domaini

Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins (By similarity).By similarity
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding (By similarity).By similarity

Sequence similaritiesi

Belongs to the importin alpha family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0166 Eukaryota
COG5064 LUCA
GeneTreeiENSGT00760000119094
HOGENOMiHOG000167616
HOVERGENiHBG001846
InParanoidiQ60960
KOiK15042
OMAiSWLLFVN
OrthoDBiEOG091G095Z
PhylomeDBiQ60960
TreeFamiTF354205

Family and domain databases

Gene3Di1.20.5.690, 1 hit
1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR032413 Arm_3
IPR000225 Armadillo
IPR002652 Importin-a_IBB
IPR036975 Importin-a_IBB_sf
IPR024931 Importing_su_alpha
PfamiView protein in Pfam
PF00514 Arm, 8 hits
PF16186 Arm_3, 1 hit
PF01749 IBB, 1 hit
PIRSFiPIRSF005673 Importin_alpha, 1 hit
SMARTiView protein in SMART
SM00185 ARM, 8 hits
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50176 ARM_REPEAT, 3 hits
PS51214 IBB, 1 hit

Sequencei

Sequence statusi: Complete.

Q60960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPGKENFR LKSYKNKSLN PDEMRRRREE EGLQLRKQKR EEQLFKRRNV
60 70 80 90 100
ATAEEETEEE VMSDGGFHEA QINNMEMAPG GVITSDMTDM IFSNSPEQQL
110 120 130 140 150
SATQKFRKLL SKEPNPPIDE VINTPGVVAR FVEFLKRKEN CTLQFESAWV
160 170 180 190 200
LTNIASGNSL QTRNVIQAGA VPIFIELLSS EFEDVQEQAV WALGNIAGDS
210 220 230 240 250
TMCRDYVLNC NILPPLLQLF SKQNRLTMTR NAVWALSNLC RGKSPPPEFA
260 270 280 290 300
KVSPCLNVLS WLLFVSDTDV LADACWALSY LSDGPNDKIQ AVIDAGVCRR
310 320 330 340 350
LVELLMHNDY KVVSPALRAV GNIVTGDDIQ TQVILNCSAL QSLLHLLSSP
360 370 380 390 400
KESIKKEACW TISNITAGNR AQIQTVIDAN MFPALISILQ TAEFRTRKEA
410 420 430 440 450
AWAITNATSG GSAEQIKYLV ELGCIKPLCD LLTVMDAKIV QVALNGLENI
460 470 480 490 500
LRLGEQEAKR NGSGINPYCA LIEEAYGLDK IEFLQSHENQ EIYQKAFDLI
510 520 530
EHYFGTEDED SSIAPQVDLS QQQYIFQQCE APMEGFQL
Length:538
Mass (Da):60,183
Last modified:July 15, 1998 - v2
Checksum:i8FE16ACA99F2BA4F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83I → T in AAC52450 (PubMed:8631802).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34228 mRNA Translation: AAC52450.1
AK028259 mRNA Translation: BAC25847.1
AK028307 mRNA Translation: BAC25872.1
AK154800 mRNA Translation: BAE32838.1
AK169311 mRNA Translation: BAE41066.1
AK169348 mRNA Translation: BAE41098.1
BC006771 mRNA Translation: AAH06771.1
U20619 mRNA No translation available.
CCDSiCCDS28144.1
RefSeqiNP_032491.2, NM_008465.5
XP_006521877.1, XM_006521814.3
UniGeneiMm.3991
Mm.6952

Genome annotation databases

EnsembliENSMUST00000004054; ENSMUSP00000004054; ENSMUSG00000022905
GeneIDi16646
KEGGimmu:16646
UCSCiuc007zcb.1 mouse

Similar proteinsi

Entry informationi

Entry nameiIMA5_MOUSE
AccessioniPrimary (citable) accession number: Q60960
Secondary accession number(s): Q3TF32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1998
Last modified: March 28, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health