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Q60954

- MEIS1_MOUSE

UniProt

Q60954 - MEIS1_MOUSE

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Protein

Homeobox protein Meis1

Gene

Meis1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a transcriptional regulator of PAX6. Also acts as a transcriptional activator of PF4 in complex with PBX1 or PBX2. Required for hematopoiesis, megakaryocyte lineage development and vascular patterning. May function as a cofactor for HOXA7 and HOXA9 in the induction of myeloid leukemias.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi272 – 33463Homeobox; TALE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: MGI
  3. protein heterodimerization activity Source: MGI
  4. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
  6. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
  7. sequence-specific DNA binding Source: MGI

GO - Biological processi

  1. angiogenesis Source: MGI
  2. blood vessel morphogenesis Source: MGI
  3. definitive hemopoiesis Source: MGI
  4. hemopoiesis Source: MGI
  5. lens morphogenesis in camera-type eye Source: MGI
  6. megakaryocyte development Source: MGI
  7. negative regulation of myeloid cell differentiation Source: UniProtKB
  8. negative regulation of neuron differentiation Source: MGI
  9. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  10. regulation of transcription, DNA-templated Source: MGI
  11. regulation of transcription from RNA polymerase II promoter Source: MGI
  12. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Meis1
Alternative name(s):
Myeloid ecotropic viral integration site 1
Gene namesi
Name:Meis1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:104717. Meis1.

Subcellular locationi

Nucleus 2 PublicationsPROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: MGI
  2. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Meis1 serves as a site of viral integration in 15% of the tumors arising in BXH-2 mice that develop myeloid leukemia as a result of the expression of an ecotropic murine leukemia virus.

Disruption phenotypei

Mice die between embryonic days 11.5 and 14.5, showing internal hemorrhage, liver hypoplasia and anemia.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi213 – 2131W → A: No effect on cooperative binding with PBX1 to CYP17. 1 Publication
Mutagenesisi321 – 3211N → S: Loss of binding to other proteins. 1 Publication

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Homeobox protein Meis1PRO_0000049106Add
BLAST

Proteomic databases

MaxQBiQ60954.
PaxDbiQ60954.
PRIDEiQ60954.

PTM databases

PhosphoSiteiQ60954.

Expressioni

Tissue specificityi

Expressed at high levels in the lung with lower levels detected in the heart and brain.

Developmental stagei

Expressed at high levels in all stages of embryonic development analyzed (7 days to 17 days).

Inductioni

Expression is coactivated by retroviral integration in BXH-2 murine myeloid leukemias.

Gene expression databases

BgeeiQ60954.
CleanExiMM_MEIS1.
ExpressionAtlasiQ60954. baseline and differential.
GenevestigatoriQ60954.

Interactioni

Subunit structurei

Interacts with the N-terminal region of PBX1 to form a heterodimer which binds DNA including a cAMP-responsive sequence in CYP17. Also forms heterodimers with PBX2. Forms heterotrimers with PBX1 or PBX2 and a number of HOX proteins including HOXA9, HOXD4 and HOXD9 where it acts as a non-DNA-binding partner. Also forms heterotrimers with PBX1 and HOX proteins including HOXD9 and HOXD10 where PBX1 is the non-DNA-binding partner.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Hoxa13Q624243EBI-445723,EBI-925160

Protein-protein interaction databases

BioGridi201386. 11 interactions.
IntActiQ60954. 13 interactions.
MINTiMINT-96479.
STRINGi10090.ENSMUSP00000099942.

Structurei

3D structure databases

ProteinModelPortaliQ60954.
SMRiQ60954. Positions 279-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni335 – 39056Required for transcriptional activationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi194 – 24047Ser/Thr-richAdd
BLAST
Compositional biasi242 – 26928Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi262 – 2698Poly-Asp

Sequence similaritiesi

Belongs to the TALE/MEIS homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG302919.
GeneTreeiENSGT00550000074260.
HOGENOMiHOG000253923.
HOVERGENiHBG055193.
InParanoidiQ60954.
KOiK15613.
OMAiVSQGAPY.
OrthoDBiEOG76HQ2W.
PhylomeDBiQ60954.
TreeFamiTF318093.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q60954-1) [UniParc]FASTAAdd to Basket

Also known as: Meis1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQRYDDLPH YGGMDGVGIP STMYGDPHAA RSMQPVHHLN HGPPLHSHQY
60 70 80 90 100
PHTAHTNAMA PSMGSSVNDA LKRDKDAIYG HPLFPLLALI FEKCELATCT
110 120 130 140 150
PREPGVAGGD VCSSESFNED IAVFAKQIRA EKPLFSSNPE LDNLMIQAIQ
160 170 180 190 200
VLRFHLLELE KVHELCDNFC HRYISCLKGK MPIDLVIDDR EGGSKSDSED
210 220 230 240 250
VTRSANLTDQ PSWNRDHDDT ASTRSGGTPG PSSGGHTSHS GDNSSEQGDG
260 270 280 290 300
LDNSVASPST GDDDDPDKDK KRHKKRGIFP KVATNIMRAW LFQHLTHPYP
310 320 330 340 350
SEEQKKQLAQ DTGLTILQVN NWFINARRRI VQPMIDQSNR AVSQGTPYNP
360 370 380 390
DGQPMGGFVM DGQQHMGIRA PGPMSGMGMN MGMEGQWHYM
Length:390
Mass (Da):43,002
Last modified:November 1, 1997 - v1
Checksum:iE0C32B5CE25E1E2C
GO
Isoform 2 (identifier: Q60954-2) [UniParc]FASTAAdd to Basket

Also known as: Meis1B

The sequence of this isoform differs from the canonical sequence as follows:
     373-390: PMSGMGMNMGMEGQWHYM → LQSMPGEYVA...SAQVMDIHAQ

Show »
Length:465
Mass (Da):50,764
Checksum:i5E1D8B911DC76E61
GO
Isoform 3 (identifier: Q60954-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     342-390: VSQGTPYNPD...MGMEGQWHYM → GKSPLVTVFKSGKRKASSSHSPGGLLPGK

Show »
Length:370
Mass (Da):40,622
Checksum:i4F3EADD4F4B9DCCA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei342 – 39049VSQGT…QWHYM → GKSPLVTVFKSGKRKASSSH SPGGLLPGK in isoform 3. 2 PublicationsVSP_017056Add
BLAST
Alternative sequencei373 – 39018PMSGM…QWHYM → LQSMPGEYVARGGPMGVSMG QPSYTQAQMPPHPAQLRHGP PMHTYIPGHPHHPAVMMHGG QPHPGMPMSASSPSVLNTGD PTMSAQVMDIHAQ in isoform 2. 1 PublicationVSP_002240Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33629 mRNA. Translation: AAA85508.1.
U33630 mRNA. Translation: AAA85509.1.
AK132298 mRNA. Translation: BAE21088.1.
AK140748 mRNA. Translation: BAE24465.1.
AL603984, AL645570 Genomic DNA. Translation: CAI24096.1.
AL603984, AL645570 Genomic DNA. Translation: CAI24097.1.
AL645570, AL603984 Genomic DNA. Translation: CAI25394.1.
AL645570, AL603984 Genomic DNA. Translation: CAI25395.1.
BC023689 mRNA. Translation: AAH23689.1.
CCDSiCCDS24453.1. [Q60954-2]
CCDS56760.1. [Q60954-1]
RefSeqiNP_001180200.1. NM_001193271.1. [Q60954-1]
NP_034919.1. NM_010789.3. [Q60954-2]
UniGeneiMm.356578.
Mm.445192.

Genome annotation databases

EnsembliENSMUST00000068264; ENSMUSP00000069277; ENSMUSG00000020160. [Q60954-1]
ENSMUST00000144988; ENSMUSP00000134969; ENSMUSG00000020160. [Q60954-3]
ENSMUST00000185131; ENSMUSP00000139219; ENSMUSG00000020160. [Q60954-2]
GeneIDi17268.
KEGGimmu:17268.
UCSCiuc007icl.2. mouse. [Q60954-2]
uc007icm.2. mouse. [Q60954-1]
uc007icn.2. mouse. [Q60954-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33629 mRNA. Translation: AAA85508.1 .
U33630 mRNA. Translation: AAA85509.1 .
AK132298 mRNA. Translation: BAE21088.1 .
AK140748 mRNA. Translation: BAE24465.1 .
AL603984 , AL645570 Genomic DNA. Translation: CAI24096.1 .
AL603984 , AL645570 Genomic DNA. Translation: CAI24097.1 .
AL645570 , AL603984 Genomic DNA. Translation: CAI25394.1 .
AL645570 , AL603984 Genomic DNA. Translation: CAI25395.1 .
BC023689 mRNA. Translation: AAH23689.1 .
CCDSi CCDS24453.1. [Q60954-2 ]
CCDS56760.1. [Q60954-1 ]
RefSeqi NP_001180200.1. NM_001193271.1. [Q60954-1 ]
NP_034919.1. NM_010789.3. [Q60954-2 ]
UniGenei Mm.356578.
Mm.445192.

3D structure databases

ProteinModelPortali Q60954.
SMRi Q60954. Positions 279-334.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201386. 11 interactions.
IntActi Q60954. 13 interactions.
MINTi MINT-96479.
STRINGi 10090.ENSMUSP00000099942.

PTM databases

PhosphoSitei Q60954.

Proteomic databases

MaxQBi Q60954.
PaxDbi Q60954.
PRIDEi Q60954.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000068264 ; ENSMUSP00000069277 ; ENSMUSG00000020160 . [Q60954-1 ]
ENSMUST00000144988 ; ENSMUSP00000134969 ; ENSMUSG00000020160 . [Q60954-3 ]
ENSMUST00000185131 ; ENSMUSP00000139219 ; ENSMUSG00000020160 . [Q60954-2 ]
GeneIDi 17268.
KEGGi mmu:17268.
UCSCi uc007icl.2. mouse. [Q60954-2 ]
uc007icm.2. mouse. [Q60954-1 ]
uc007icn.2. mouse. [Q60954-3 ]

Organism-specific databases

CTDi 4211.
MGIi MGI:104717. Meis1.

Phylogenomic databases

eggNOGi NOG302919.
GeneTreei ENSGT00550000074260.
HOGENOMi HOG000253923.
HOVERGENi HBG055193.
InParanoidi Q60954.
KOi K15613.
OMAi VSQGAPY.
OrthoDBi EOG76HQ2W.
PhylomeDBi Q60954.
TreeFami TF318093.

Miscellaneous databases

ChiTaRSi Meis1. mouse.
NextBioi 291766.
PROi Q60954.
SOURCEi Search...

Gene expression databases

Bgeei Q60954.
CleanExi MM_MEIS1.
ExpressionAtlasi Q60954. baseline and differential.
Genevestigatori Q60954.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
InterProi IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
[Graphical view ]
Pfami PF05920. Homeobox_KN. 1 hit.
[Graphical view ]
SMARTi SM00389. HOX. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS50071. HOMEOBOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Meis1, a PBX1-related homeobox gene involved in myeloid leukemia in BXH-2 mice."
    Moskow J.J., Bullrich F., Huebner K., Daar I.O., Buchberg A.M.
    Mol. Cell. Biol. 15:5434-5443(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J.
    Tissue: Embryo and Head.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "Meis proteins are major in vivo DNA binding partners for wild-type but not chimeric Pbx proteins."
    Chang C.-P., Jacobs Y., Nakamura T., Jenkins N.A., Copeland N.G., Cleary M.L.
    Mol. Cell. Biol. 17:5679-5687(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PBX1.
  6. "Members of the Meis1 and Pbx homeodomain protein families cooperatively bind a cAMP-responsive sequence (CRS1) from bovine CYP17."
    Bischof L.J., Kagawa N., Moskow J.J., Takahashi Y., Iwamatsu A., Buchberg A.M., Waterman M.R.
    J. Biol. Chem. 273:7941-7948(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PBX1, MUTAGENESIS OF TRP-213.
  7. "HOXA9 forms triple complexes with PBX2 and MEIS1 in myeloid cells."
    Shen W.-F., Rozenfeld S., Kwong A., Koemueves L.G., Lawrence H.J., Largman C.
    Mol. Cell. Biol. 19:3051-3061(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HOXA9; PBX1 AND PBX2, SUBCELLULAR LOCATION.
  8. "PBX and MEIS as non-DNA-binding partners in trimeric complexes with HOX proteins."
    Shanmugam K., Green N.C., Rambaldi I., Saragovi H.U., Featherstone M.S.
    Mol. Cell. Biol. 19:7577-7588(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PBX1; HOXD4; HOXD9 AND HOXD10, MUTAGENESIS OF ASN-321.
  9. "Meis homeoproteins directly regulate Pax6 during vertebrate lens morphogenesis."
    Zhang X., Friedman A., Heaney S., Purcell P., Maas R.L.
    Genes Dev. 16:2097-2107(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "The homeodomain protein Meis1 is essential for definitive hematopoiesis and vascular patterning in the mouse embryo."
    Azcoitia V., Aracil M., Martinez-A C., Torres M.
    Dev. Biol. 280:307-320(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  11. "MEIS C termini harbor transcriptional activation domains that respond to cell signaling."
    Huang H., Rastegar M., Bodner C., Goh S.-L., Rambaldi I., Featherstone M.
    J. Biol. Chem. 280:10119-10127(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSCRIPTIONAL ACTIVATION DOMAIN.

Entry informationi

Entry nameiMEIS1_MOUSE
AccessioniPrimary (citable) accession number: Q60954
Secondary accession number(s): B1ARH5
, Q5SVC8, Q60955, Q8CIL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3