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Protein

TBC1 domain family member 1

Gene

Tbc1d1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues. Involved in the trafficking and translocation of GLUT4-containing vesicles and insulin-stimulated glucose uptake into cells.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
TBC1 domain family member 1
Gene namesi
Name:Tbc1d1
Synonyms:Kiaa1108, Tbc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1889508. Tbc1d1.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002080231 – 1255TBC1 domain family member 1Add BLAST1255

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei146Phosphoserine1 Publication1
Modified residuei229Phosphoserine; by PKB/AKT11 Publication1
Modified residuei231Phosphoserine; by AMPK1 Publication1
Modified residuei489Phosphoserine; by PKB/AKT11 Publication1
Modified residuei497PhosphoserineCombined sources1 Publication1
Modified residuei499Phosphothreonine; by PKB/AKT11 Publication1
Modified residuei501PhosphoserineCombined sources1 Publication1
Modified residuei519Phosphoserine1 Publication1
Modified residuei521Phosphoserine1 Publication1
Modified residuei559PhosphoserineCombined sources1 Publication1
Modified residuei560PhosphoserineCombined sources1
Modified residuei564PhosphoserineBy similarity1
Modified residuei565Phosphoserine1 Publication1
Modified residuei579PhosphoserineBy similarity1
Modified residuei590PhosphothreonineCombined sources1 Publication1
Modified residuei608Phosphoserine1 Publication1
Modified residuei621Phosphoserine; by PKB/AKT1Combined sources1 Publication1
Modified residuei660PhosphoserineCombined sources1 Publication1
Modified residuei661Phosphoserine1 Publication1
Modified residuei697Phosphoserine; by PKB/AKT11 Publication1
Modified residuei698Phosphoserine1 Publication1
Modified residuei699Phosphoserine1 Publication1
Modified residuei700Phosphoserine; by AMPKCombined sources1 Publication1
Modified residuei782PhosphoserineCombined sources1
Modified residuei1028Phosphoserine1 Publication1
Modified residuei1039Phosphotyrosine1 Publication1
Modified residuei1218Phosphothreonine1 Publication1

Post-translational modificationi

Insulin-stimulated phosphorylation by AKT family kinases stimulates SLC2A4/GLUT4 translocation.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ60949.
PRIDEiQ60949.

PTM databases

iPTMnetiQ60949.
PhosphoSitePlusiQ60949.

Expressioni

Tissue specificityi

Expressed in highest levels in hematopoietic cells, testis and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000029174.
CleanExiMM_TBC1D1.
ExpressionAtlasiQ60949. baseline and differential.
GenevisibleiQ60949. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000098756.

Structurei

3D structure databases

ProteinModelPortaliQ60949.
SMRiQ60949.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini238 – 398PIDPROSITE-ProRule annotationAdd BLAST161
Domaini887 – 1081Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST195

Sequence similaritiesi

Contains 1 PID domain.PROSITE-ProRule annotation
Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4436. Eukaryota.
ENOG410YWJY. LUCA.
GeneTreeiENSGT00750000117238.
HOGENOMiHOG000090019.
HOVERGENiHBG059376.
InParanoidiQ60949.
KOiK18341.
OMAiKFNHVSC.
OrthoDBiEOG091G01QW.
TreeFamiTF317184.

Family and domain databases

Gene3Di2.30.29.30. 3 hits.
InterProiIPR021785. DUF3350.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
IPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF11830. DUF3350. 1 hit.
PF00640. PID. 1 hit.
PF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 2 hits.
SM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
SSF50729. SSF50729. 3 hits.
PROSITEiPS01179. PID. 1 hit.
PS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q60949-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAITFTARK HPFPNEVSVD FGLQLVGSLP VHSLTTMPML PWVVAEVRRL
60 70 80 90 100
SGQCSKKEPR TKQVRLWVSP SGLRCEPDLE KSQPWDPLIC SSIFECKPQR
110 120 130 140 150
VHKLIHNSHD PSYFACLIKE DAAHRQSLCY VFKADDQTKV PEIISSIRQA
160 170 180 190 200
GKIARQEELR CPSEFDDTFA KKFEVLFCGR VTVAHKKAPP ALIDECIEKF
210 220 230 240 250
NHVSCGRRTD WEAPTGQPSA PGPRPMRKSF SQPGLRSLAF RKEFQDASLR
260 270 280 290 300
SSTFSSFDND IENHLIGGHN VVQPTDMEEN RTMLFTIGQS EVYLISPDTK
310 320 330 340 350
KIALEKNFKE ISFCSQGIRH VDHFGFICRE CSGGGSGGFH FVCYVFQCTN
360 370 380 390 400
EALVDEIMMT LKQAFTVAAV QQTAKAPAQL CEGCPLQGLH KLCERIEGMN
410 420 430 440 450
SSKTKLELQK HLTTLTNQEQ ATIFEEVQKL RPRNEQRENE LIISFLRCLY
460 470 480 490 500
EEKQKEHSHT GEPKQTLQVA AENIGSDLPP SASRFRLDSL KNRAKRSLTE
510 520 530 540 550
SLESILSRGN KARGLQDHSA SVDLDSSTSS TLSNTSKELS MGDKEAFPVS
560 570 580 590 600
ETSFKLLGSS DDLSSDSEGH IAEESALLSP QQAFRRRANT LSHFPVECPA
610 620 630 640 650
PPEPAQSSPG VSQRKLMRYH SVSTETPHER NVDHLPGGES QGCPGQPSAP
660 670 680 690 700
PPPRLNPSAS SPNFFKYLKH NSSGEQSGNA VPKSVSYRNA LRKKLHSSSS
710 720 730 740 750
VPNFLKFLAP VDENNTCDFK NTNRDFESKA NHLGDTDGTP VKTRRHSWRQ
760 770 780 790 800
QIFLRVATPQ KACDSPSRYE DYSELGELPP RSPLEPVCED GPFGPVQEEK
810 820 830 840 850
RKTSRELREL WKKAILQQIL LLRMEKENQK LQASENDLLN KRLKLDYEEI
860 870 880 890 900
TPCLKEVTTV WEKMLSTPGR SKIKFDMEKV HSAVGQGVPR HHRGEIWKFL
910 920 930 940 950
AEQFHLKHPF PSKQQPKDVP YKELLKKLTS QQHAILIDLG RTFPTHPYFS
960 970 980 990 1000
AQLGAGQLSL YNILKAYSLL DQEVGYCQGL SFVAGILLLH MSEEEAFKML
1010 1020 1030 1040 1050
KFLMFDMGLR KQYRPDMIIL QIQMYQLSRL LHDYHRDLYN HLEEHEIGPS
1060 1070 1080 1090 1100
LYAAPWFLTV FASQFPLGFV ARVFDMIFLQ GSEVIFKVAL SLLGSHKPLI
1110 1120 1130 1140 1150
LQHENLETIV DFIKNTLPNL GLVQMEKTIS QVFEMDIAKQ LQAYEVEYHV
1160 1170 1180 1190 1200
LQEELIESSP LSDNQRMEKL EKTNSSLRKQ NLDLLEQLQV ANARIQSLEA
1210 1220 1230 1240 1250
TVEKLLTSES KLKQAALTLE VERSALLQMV EELRRQSARP STPEPDCTQL

EPTGD
Note: No experimental confirmation available.
Length:1,255
Mass (Da):142,026
Last modified:July 27, 2011 - v3
Checksum:i8AC3121224B310D2
GO
Isoform 2 (identifier: Q60949-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     631-724: NVDHLPGGES...NTCDFKNTNR → K

Show »
Length:1,162
Mass (Da):132,008
Checksum:iA839A42715918549
GO

Sequence cautioni

The sequence AAA85223 differs from that shown. Reason: Frameshift at positions 15, 30, 795, 822 and 1224.Curated
The sequence BAC65727 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti174E → K in BAC65727 (PubMed:12693553).Curated1
Sequence conflicti289Q → P in AAA85223 (PubMed:7566974).Curated1
Sequence conflicti462E → A in AAA85223 (PubMed:7566974).Curated1
Sequence conflicti848E → D in AAH04675 (PubMed:15489334).Curated1
Sequence conflicti1047I → T in AAA85223 (PubMed:7566974).Curated1
Sequence conflicti1050 – 1051SL → PT in AAA85223 (PubMed:7566974).Curated2
Sequence conflicti1143A → P in AAH04675 (PubMed:15489334).Curated1
Sequence conflicti1151L → V in AAA85223 (PubMed:7566974).Curated1
Sequence conflicti1176S → T in AAA85223 (PubMed:7566974).Curated1
Sequence conflicti1215A → R in AAA85223 (PubMed:7566974).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008473631 – 724NVDHL…KNTNR → K in isoform 2. 1 PublicationAdd BLAST94

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33005 mRNA. Translation: AAA85223.1. Frameshift.
AK122445 mRNA. Translation: BAC65727.1. Different initiation.
AC131679 Genomic DNA. No translation available.
AC132271 Genomic DNA. No translation available.
BC004675 mRNA. Translation: AAH04675.1.
CCDSiCCDS39094.1. [Q60949-2]
CCDS80287.1. [Q60949-1]
PIRiT29104.
RefSeqiNP_001297540.1. NM_001310611.1. [Q60949-1]
XP_011239059.1. XM_011240757.2. [Q60949-1]
XP_011239060.1. XM_011240758.2. [Q60949-1]
UniGeneiMm.286353.

Genome annotation databases

EnsembliENSMUST00000043893; ENSMUSP00000044577; ENSMUSG00000029174. [Q60949-1]
ENSMUST00000101195; ENSMUSP00000098756; ENSMUSG00000029174. [Q60949-2]
ENSMUST00000121370; ENSMUSP00000112493; ENSMUSG00000029174. [Q60949-2]
GeneIDi57915.
KEGGimmu:57915.
UCSCiuc008xmm.2. mouse. [Q60949-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33005 mRNA. Translation: AAA85223.1. Frameshift.
AK122445 mRNA. Translation: BAC65727.1. Different initiation.
AC131679 Genomic DNA. No translation available.
AC132271 Genomic DNA. No translation available.
BC004675 mRNA. Translation: AAH04675.1.
CCDSiCCDS39094.1. [Q60949-2]
CCDS80287.1. [Q60949-1]
PIRiT29104.
RefSeqiNP_001297540.1. NM_001310611.1. [Q60949-1]
XP_011239059.1. XM_011240757.2. [Q60949-1]
XP_011239060.1. XM_011240758.2. [Q60949-1]
UniGeneiMm.286353.

3D structure databases

ProteinModelPortaliQ60949.
SMRiQ60949.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000098756.

PTM databases

iPTMnetiQ60949.
PhosphoSitePlusiQ60949.

Proteomic databases

PaxDbiQ60949.
PRIDEiQ60949.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043893; ENSMUSP00000044577; ENSMUSG00000029174. [Q60949-1]
ENSMUST00000101195; ENSMUSP00000098756; ENSMUSG00000029174. [Q60949-2]
ENSMUST00000121370; ENSMUSP00000112493; ENSMUSG00000029174. [Q60949-2]
GeneIDi57915.
KEGGimmu:57915.
UCSCiuc008xmm.2. mouse. [Q60949-1]

Organism-specific databases

CTDi23216.
MGIiMGI:1889508. Tbc1d1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4436. Eukaryota.
ENOG410YWJY. LUCA.
GeneTreeiENSGT00750000117238.
HOGENOMiHOG000090019.
HOVERGENiHBG059376.
InParanoidiQ60949.
KOiK18341.
OMAiKFNHVSC.
OrthoDBiEOG091G01QW.
TreeFamiTF317184.

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

ChiTaRSiTbc1d1. mouse.
PROiQ60949.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029174.
CleanExiMM_TBC1D1.
ExpressionAtlasiQ60949. baseline and differential.
GenevisibleiQ60949. MM.

Family and domain databases

Gene3Di2.30.29.30. 3 hits.
InterProiIPR021785. DUF3350.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
IPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF11830. DUF3350. 1 hit.
PF00640. PID. 1 hit.
PF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 2 hits.
SM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
SSF50729. SSF50729. 3 hits.
PROSITEiPS01179. PID. 1 hit.
PS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTBCD1_MOUSE
AccessioniPrimary (citable) accession number: Q60949
Secondary accession number(s): E9QLW9, Q80TJ9, Q923F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.