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Q60948

- MAD4_MOUSE

UniProt

Q60948 - MAD4_MOUSE

Protein

Max dimerization protein 4

Gene

Mxd4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.1 Publication

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: MGI

    GO - Biological processi

    1. negative regulation of transcription, DNA-templated Source: MGI
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Max dimerization protein 4
    Short name:
    Max dimerizer 4
    Alternative name(s):
    Max-associated protein 4
    Max-interacting transcriptional repressor MAD4
    Gene namesi
    Name:Mxd4
    Synonyms:Mad4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:104991. Mxd4.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 209209Max dimerization protein 4PRO_0000127267Add
    BLAST

    Proteomic databases

    PRIDEiQ60948.

    PTM databases

    PhosphoSiteiQ60948.

    Expressioni

    Developmental stagei

    Expressed during neural and epidermal differentiation. Primarily expressed in growth-arrested differentiating cells. In the spinal cord at embryonic day 10.5, a strong expression seen in the differentiating cells of the intermediate zone at the ventral part of the neural tube and weakly in the ventricular zone. At 11.5 and 12.5 dpc, highly expressed in the intermediate zone and at reduced levels in the ventricular zone that mostly persists in the dorsal part of the neural tube. At 14.5 dpc, expressed throughout the spinal cord. In the developing epidermis, expressed in the dermis, hair follicles and in some differentiating cells in the upper layers of the epidermis.1 Publication

    Gene expression databases

    CleanExiMM_MXD4.
    GenevestigatoriQ60948.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SIN3A AND SIN3B. Interacts with RNF17.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliQ60948.
    SMRiQ60948. Positions 54-132.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini53 – 10553bHLHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni6 – 2318Interaction with SIN3A and SIN3BAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG244990.
    HOVERGENiHBG006314.
    InParanoidiQ60948.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60948-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELNSLLLLL EAAEYLERRD REAEHGYASM LPFDGDFARK KTKTAGLVRK    50
    GPNNRSSHNE LEKHRRAKLR LYLEQLKQLG PLGPDSTRHT TLSLLKRAKM 100
    HIKKLEEQDR RALSIKEQLQ REHRFLKRRL EQLSVQSVER VRTDSTGSAV 150
    STDDSEQEVD IEGMEFGPGE LDSAGSSSDA DDHYSLQSSG CSDSSYGHPC 200
    RRPGCPGLS 209
    Length:209
    Mass (Da):23,590
    Last modified:November 1, 1996 - v1
    Checksum:iC8FB5A56AFE3B27C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32395 mRNA. Translation: AAB02795.1.
    CCDSiCCDS19212.1.
    UniGeneiMm.259260.
    Mm.391777.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32395 mRNA. Translation: AAB02795.1 .
    CCDSi CCDS19212.1.
    UniGenei Mm.259260.
    Mm.391777.

    3D structure databases

    ProteinModelPortali Q60948.
    SMRi Q60948. Positions 54-132.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q60948.

    Proteomic databases

    PRIDEi Q60948.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:104991. Mxd4.

    Phylogenomic databases

    eggNOGi NOG244990.
    HOVERGENi HBG006314.
    InParanoidi Q60948.

    Miscellaneous databases

    PROi Q60948.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_MXD4.
    Genevestigatori Q60948.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation."
      Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N.
      EMBO J. 14:5646-5659(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBUNIT, INTERACTION WITH SIN3A AND SIN3B.
      Strain: AB1.
      Tissue: Embryonic stem cell.
    2. "Mmip-2, a novel RING finger protein that interacts with mad members of the Myc oncoprotein network."
      Yin X.-Y., Gupta K., Prochownik E.V.
      Oncogene 18:6621-6634(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RNF17.

    Entry informationi

    Entry nameiMAD4_MOUSE
    AccessioniPrimary (citable) accession number: Q60948
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3