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Q60948

- MAD4_MOUSE

UniProt

Q60948 - MAD4_MOUSE

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Protein

Max dimerization protein 4

Gene

Mxd4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.1 Publication

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: MGI
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Max dimerization protein 4
Short name:
Max dimerizer 4
Alternative name(s):
Max-associated protein 4
Max-interacting transcriptional repressor MAD4
Gene namesi
Name:Mxd4
Synonyms:Mad4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:104991. Mxd4.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Max dimerization protein 4PRO_0000127267Add
BLAST

Proteomic databases

PRIDEiQ60948.

PTM databases

PhosphoSiteiQ60948.

Expressioni

Developmental stagei

Expressed during neural and epidermal differentiation. Primarily expressed in growth-arrested differentiating cells. In the spinal cord at embryonic day 10.5, a strong expression seen in the differentiating cells of the intermediate zone at the ventral part of the neural tube and weakly in the ventricular zone. At 11.5 and 12.5 dpc, highly expressed in the intermediate zone and at reduced levels in the ventricular zone that mostly persists in the dorsal part of the neural tube. At 14.5 dpc, expressed throughout the spinal cord. In the developing epidermis, expressed in the dermis, hair follicles and in some differentiating cells in the upper layers of the epidermis.1 Publication

Gene expression databases

CleanExiMM_MXD4.
GenevestigatoriQ60948.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SIN3A AND SIN3B. Interacts with RNF17.2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ60948.
SMRiQ60948. Positions 54-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 10553bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni6 – 2318Interaction with SIN3A and SIN3BAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG244990.
HOVERGENiHBG006314.
InParanoidiQ60948.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60948-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELNSLLLLL EAAEYLERRD REAEHGYASM LPFDGDFARK KTKTAGLVRK
60 70 80 90 100
GPNNRSSHNE LEKHRRAKLR LYLEQLKQLG PLGPDSTRHT TLSLLKRAKM
110 120 130 140 150
HIKKLEEQDR RALSIKEQLQ REHRFLKRRL EQLSVQSVER VRTDSTGSAV
160 170 180 190 200
STDDSEQEVD IEGMEFGPGE LDSAGSSSDA DDHYSLQSSG CSDSSYGHPC

RRPGCPGLS
Length:209
Mass (Da):23,590
Last modified:November 1, 1996 - v1
Checksum:iC8FB5A56AFE3B27C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32395 mRNA. Translation: AAB02795.1.
CCDSiCCDS19212.1.
UniGeneiMm.259260.
Mm.391777.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32395 mRNA. Translation: AAB02795.1 .
CCDSi CCDS19212.1.
UniGenei Mm.259260.
Mm.391777.

3D structure databases

ProteinModelPortali Q60948.
SMRi Q60948. Positions 54-132.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q60948.

Proteomic databases

PRIDEi Q60948.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:104991. Mxd4.

Phylogenomic databases

eggNOGi NOG244990.
HOVERGENi HBG006314.
InParanoidi Q60948.

Miscellaneous databases

PROi Q60948.
SOURCEi Search...

Gene expression databases

CleanExi MM_MXD4.
Genevestigatori Q60948.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
[Graphical view ]
Pfami PF00010. HLH. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation."
    Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N.
    EMBO J. 14:5646-5659(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBUNIT, INTERACTION WITH SIN3A AND SIN3B.
    Strain: AB1.
    Tissue: Embryonic stem cell.
  2. "Mmip-2, a novel RING finger protein that interacts with mad members of the Myc oncoprotein network."
    Yin X.-Y., Gupta K., Prochownik E.V.
    Oncogene 18:6621-6634(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RNF17.

Entry informationi

Entry nameiMAD4_MOUSE
AccessioniPrimary (citable) accession number: Q60948
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3