Reviewed, UniProtKB/Swiss-Prot Q60948 (MAD4_MOUSE)

Names and origin

Protein names

Recommended name:
    Max-interacting transcriptional repressor MAD4
Alternative name(s):
    Max-associated protein 4
    MAX dimerization protein 4

Gene names

Name:	Mxd4
Synonyms:	Mad4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation. Ref.1
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SIN3A AND SIN3B. Interacts with RNF17. Ref.1 Ref.3
Subcellular location	Nucleus.
Developmental stage	Expressed during neural and epidermal differentiation. Primarily expressed in growth-arrested differentiating cells. In the spinal cord at embryonic day 10.5, a strong expression seen in the differentiating cells of the intermediate zone at the ventral part of the neural tube and weakly in the ventricular zone. At 11.5 and 12.5 dpc, highly expressed in the intermediate zone and at reduced levels in the ventricular zone that mostly persists in the dorsal part of the neural tube. At 14.5 dpc, expressed throughout the spinal cord. In the developing epidermis, expressed in the dermis, hair follicles and in some differentiating cells in the upper layers of the epidermis. Ref.1
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Repressor
Gene Ontology (GO)
Biological process	negative regulation of transcription Ref.1 Inferred from genetic interaction. Source: MGI regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Ref.1 Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.1 Inferred from physical interaction. Source: MGI transcription regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Max-interacting transcriptional repressor MAD4		PRO_0000127267
Regions
	Domain	67 – 106	40	Helix-loop-helix motif
	DNA binding	55 – 66	12	Basic motif
	Region	6 – 23	18	Interaction with SIN3A and SIN3B

Sequences

	Sequence		Length	Mass (Da)
	Q60948-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C8FB5A56AFE3B27C	FASTA	209	23,590
	10 20 30 40 50 60 MELNSLLLLL EAAEYLERRD REAEHGYASM LPFDGDFARK KTKTAGLVRK GPNNRSSHNE 70 80 90 100 110 120 LEKHRRAKLR LYLEQLKQLG PLGPDSTRHT TLSLLKRAKM HIKKLEEQDR RALSIKEQLQ 130 140 150 160 170 180 REHRFLKRRL EQLSVQSVER VRTDSTGSAV STDDSEQEVD IEGMEFGPGE LDSAGSSSDA 190 200 DDHYSLQSSG CSDSSYGHPC RRPGCPGLS

References

[1]	"Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation." Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N. EMBO J. 14:5646-5659(1995) [PubMed: 8521822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBUNIT, INTERACTION WITH SIN3A AND SIN3B. Strain: AB1. Tissue: Embryonic stem cell.
[2]	Erratum Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N. EMBO J. 15:2030-2030(1996) [PubMed: 8617250] [Abstract]
[3]	"Mmip-2, a novel RING finger protein that interacts with mad members of the Myc oncoprotein network." Yin X.-Y., Gupta K., Prochownik E.V. Oncogene 18:6621-6634(1999) [PubMed: 10597267] [Abstract] Cited for: INTERACTION WITH RNF17.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U32395 mRNA. Translation: AAB02795.1.
IPI	IPI00331243.
UniGene	Mm.259260 Mm.391777
3D structure databases
HSSP	HSSP built from PDB template 1NLW based on UniProtKB Q05195.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000037235. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:104991. Mxd4.
Phylogenomic databases
HOVERGEN	Q60948.
Gene expression databases
ArrayExpress	Q60948.
Bgee	Q60948.
CleanEx	MM_MXD4.
GermOnline	ENSMUSG00000037235. Mus musculus.
Family and domain databases
InterPro	IPR001092. HLH_basic. IPR011598. HLH_DNA_bd. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
PROSITE	PS50888. HLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

MAD4_MOUSE

Accession

Primary (citable) accession number: Q60948

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)