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Q60948 (MAD4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Max dimerization protein 4

Short name=Max dimerizer 4
Alternative name(s):
Max-associated protein 4
Max-interacting transcriptional repressor MAD4
Gene names
Name:Mxd4
Synonyms:Mad4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length209 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation. Ref.1

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SIN3A AND SIN3B. Interacts with RNF17. Ref.1 Ref.3

Subcellular location

Nucleus.

Developmental stage

Expressed during neural and epidermal differentiation. Primarily expressed in growth-arrested differentiating cells. In the spinal cord at embryonic day 10.5, a strong expression seen in the differentiating cells of the intermediate zone at the ventral part of the neural tube and weakly in the ventricular zone. At 11.5 and 12.5 dpc, highly expressed in the intermediate zone and at reduced levels in the ventricular zone that mostly persists in the dorsal part of the neural tube. At 14.5 dpc, expressed throughout the spinal cord. In the developing epidermis, expressed in the dermis, hair follicles and in some differentiating cells in the upper layers of the epidermis. Ref.1

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionRepressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnegative regulation of transcription, DNA-templated

Inferred from genetic interaction Ref.1. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 10229200Ref.1. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 209209Max dimerization protein 4
PRO_0000127267

Regions

Domain53 – 10553bHLH
Region6 – 2318Interaction with SIN3A and SIN3B

Sequences

Sequence LengthMass (Da)Tools
Q60948 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C8FB5A56AFE3B27C

FASTA20923,590
        10         20         30         40         50         60 
MELNSLLLLL EAAEYLERRD REAEHGYASM LPFDGDFARK KTKTAGLVRK GPNNRSSHNE 

        70         80         90        100        110        120 
LEKHRRAKLR LYLEQLKQLG PLGPDSTRHT TLSLLKRAKM HIKKLEEQDR RALSIKEQLQ 

       130        140        150        160        170        180 
REHRFLKRRL EQLSVQSVER VRTDSTGSAV STDDSEQEVD IEGMEFGPGE LDSAGSSSDA 

       190        200 
DDHYSLQSSG CSDSSYGHPC RRPGCPGLS 

« Hide

References

[1]"Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation."
Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N.
EMBO J. 14:5646-5659(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBUNIT, INTERACTION WITH SIN3A AND SIN3B.
Strain: AB1.
Tissue: Embryonic stem cell.
[2]Erratum
Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N.
EMBO J. 15:2030-2030(1996) [PubMed] [Europe PMC] [Abstract]
[3]"Mmip-2, a novel RING finger protein that interacts with mad members of the Myc oncoprotein network."
Yin X.-Y., Gupta K., Prochownik E.V.
Oncogene 18:6621-6634(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RNF17.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U32395 mRNA. Translation: AAB02795.1.
CCDSCCDS19212.1.
UniGeneMm.259260.
Mm.391777.

3D structure databases

ProteinModelPortalQ60948.
SMRQ60948. Positions 54-132.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ60948.

Proteomic databases

PRIDEQ60948.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:104991. Mxd4.

Phylogenomic databases

eggNOGNOG244990.
HOVERGENHBG006314.
InParanoidQ60948.

Gene expression databases

CleanExMM_MXD4.
GenevestigatorQ60948.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
[Graphical view]
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ60948.
SOURCESearch...

Entry information

Entry nameMAD4_MOUSE
AccessionPrimary (citable) accession number: Q60948
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot