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Protein

Atypical kinase ADCK3, mitochondrial

Gene

Adck3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration. Its substrate specificity is unclear: either acts as protein kinase that phosphorylates other proteins in the CoQ complex to stabilize their interactions or acts as a small molecule kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway. Shows an unusual selectivity for binding ADP over ATP.By similarity

Enzyme regulationi

Autoinhibited by the N-terminal domain, containing the KxGQ motif, that completely occludes the typical substrate binding pocket.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei355 – 3551ATPBy similarity
Active sitei485 – 4851Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi333 – 3419ATPBy similarity

GO - Molecular functioni

  1. ADP binding Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. kinase activity Source: UniProtKB

GO - Biological processi

  1. phosphorylation Source: UniProtKB
  2. ubiquinone biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubiquinone biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
Atypical kinase ADCK3, mitochondrialCurated (EC:2.7.-.-By similarity)
Alternative name(s):
Chaperone activity of bc1 complex-likeBy similarity
Short name:
Chaperone-ABC1-likeBy similarity
aarF domain-containing protein kinase 3Imported
Gene namesi
Name:Adck3Imported
Synonyms:Cabc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1914676. Adck3.

Subcellular locationi

  1. Mitochondrion By similarity
  2. Membrane Sequence Analysis; Single-pass membrane protein Sequence AnalysisBy similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei211 – 22717HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 159159MitochondrionBy similarityAdd
BLAST
Chaini160 – 645486Atypical kinase ADCK3, mitochondrialPRO_0000000263Add
BLAST

Proteomic databases

MaxQBiQ60936.
PaxDbiQ60936.
PRIDEiQ60936.

PTM databases

PhosphoSiteiQ60936.

Expressioni

Gene expression databases

BgeeiQ60936.
ExpressionAtlasiQ60936. baseline and differential.
GenevestigatoriQ60936.

Interactioni

Subunit structurei

Homodimer; homodimerizes via its transmembrane region.By similarity

Protein-protein interaction databases

IntActiQ60936. 1 interaction.
MINTiMINT-1859515.

Structurei

3D structure databases

ProteinModelPortaliQ60936.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini326 – 515190Protein kinaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi273 – 2764KxGQ motifBy similarity
Motifi334 – 3374AAAS motifBy similarity

Domaini

Adopts an atypical protein kinase-like fold: while it adopts a core fold similar to that of well-characterized protein kinase-like domains, a number of features are positioned to inhibit the kinase activity: (1) an atypical AAAS motif in an alanine-rich (A-rich) loop that replaces the canonical glycine-rich (G-rich) nucleotide-binding loop and limits ATP binding by establishing an unusual selectivity for ADP and (2) an N-terminal domain, containing the KxGQ motif, that completely occludes the typical substrate binding pocket.By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0661.
GeneTreeiENSGT00550000074739.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
InParanoidiQ60936.
KOiK08869.
OMAiKVQGQDK.
OrthoDBiEOG7X9G6H.
PhylomeDBiQ60936.
TreeFamiTF300630.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q60936-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAMLGDAIM VAKGLAKLTQ AAVETHLQNL GLGGELLLAA RALQSTAVEQ
60 70 80 90 100
FSMVFGKVQG QDKHEDSYAT ENFEDLEAEV QFSTPQAAGT SLDFSAASSL
110 120 130 140 150
DQSLSPSHSQ GPAPAYASSG PFREAGLPGQ ATSPMGRVNG RLFVDHRDLF
160 170 180 190 200
LANGIQRRSF HQDQSSVGGL TAEDIEKARQ AKARPESKPH KQMLSERARE
210 220 230 240 250
RKVPVTRIGR LANFGGLAVG LGIGALAEVA KKSLRSENST GKKAVLDSSP
260 270 280 290 300
FLSEANAERI VSTLCKVRGA ALKLGQMLSI QDDAFINPHL AKIFERVRQS
310 320 330 340 350
ADFMPLKQMT KTLNSDLGPH WRDKLEYFEE RPFAAASIGQ VHLARMKGGR
360 370 380 390 400
EVAMKIQYPG VAQSINSDVN NLMAVLNMSN MLPEGLFPEH LIDVLRRELT
410 420 430 440 450
LECDYQREAA YAKKFRELLK DHPFFYVPEI VDELCSPHVL TTELISGFPL
460 470 480 490 500
DQAEGLSQEV RNEICYNILV LCLRELFEFH VMQTDPNWSN FFYDPQQHKV
510 520 530 540 550
ALLDFGATRE YDRSFTDLYI QVIRAAADQD REAVLKKSIE MKFLTGYEVK
560 570 580 590 600
AMEDAHLDAI LILGEAFASE EPFDFGTQST TEKIHNLIPV MLKHRLIPPP
610 620 630 640
EETYSLHRKM GGSFLICSKL KARFPCKAMF EEAYSNYCRM KSGLQ
Length:645
Mass (Da):71,743
Last modified:January 27, 2003 - v2
Checksum:iC94854B41211F880
GO
Isoform 2 (identifier: Q60936-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-276: Missing.

Note: No experimental confirmation available.

Show »
Length:369
Mass (Da):42,564
Checksum:iE4CB1DDCC02B4309
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti213 – 2131N → D in BAB29459 (PubMed:16141072).Curated
Sequence conflicti388 – 3881P → S in BAB29459 (PubMed:16141072).Curated
Sequence conflicti546 – 5472GY → RP in AAA86413 (Ref. 4) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 276276Missing in isoform 2. 1 PublicationVSP_022354Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278735 mRNA. Translation: CAC00538.1.
AK004791 mRNA. Translation: BAB23567.2.
AK014605 mRNA. Translation: BAB29459.2.
U31629 mRNA. Translation: AAA86413.1.
CCDSiCCDS15566.1. [Q60936-1]
RefSeqiNP_001156762.1. NM_001163290.1. [Q60936-1]
NP_075830.2. NM_023341.3. [Q60936-1]
XP_006497024.1. XM_006496961.1. [Q60936-1]
XP_006497025.1. XM_006496962.1. [Q60936-1]
UniGeneiMm.38330.

Genome annotation databases

EnsembliENSMUST00000027766; ENSMUSP00000027766; ENSMUSG00000026489. [Q60936-1]
ENSMUST00000170472; ENSMUSP00000128290; ENSMUSG00000026489. [Q60936-1]
GeneIDi67426.
KEGGimmu:67426.
UCSCiuc007dvx.2. mouse. [Q60936-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278735 mRNA. Translation: CAC00538.1.
AK004791 mRNA. Translation: BAB23567.2.
AK014605 mRNA. Translation: BAB29459.2.
U31629 mRNA. Translation: AAA86413.1.
CCDSiCCDS15566.1. [Q60936-1]
RefSeqiNP_001156762.1. NM_001163290.1. [Q60936-1]
NP_075830.2. NM_023341.3. [Q60936-1]
XP_006497024.1. XM_006496961.1. [Q60936-1]
XP_006497025.1. XM_006496962.1. [Q60936-1]
UniGeneiMm.38330.

3D structure databases

ProteinModelPortaliQ60936.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ60936. 1 interaction.
MINTiMINT-1859515.

PTM databases

PhosphoSiteiQ60936.

Proteomic databases

MaxQBiQ60936.
PaxDbiQ60936.
PRIDEiQ60936.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027766; ENSMUSP00000027766; ENSMUSG00000026489. [Q60936-1]
ENSMUST00000170472; ENSMUSP00000128290; ENSMUSG00000026489. [Q60936-1]
GeneIDi67426.
KEGGimmu:67426.
UCSCiuc007dvx.2. mouse. [Q60936-1]

Organism-specific databases

CTDi56997.
MGIiMGI:1914676. Adck3.

Phylogenomic databases

eggNOGiCOG0661.
GeneTreeiENSGT00550000074739.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
InParanoidiQ60936.
KOiK08869.
OMAiKVQGQDK.
OrthoDBiEOG7X9G6H.
PhylomeDBiQ60936.
TreeFamiTF300630.

Enzyme and pathway databases

UniPathwayiUPA00232.

Miscellaneous databases

ChiTaRSiAdck3. mouse.
NextBioi324532.
PROiQ60936.
SOURCEiSearch...

Gene expression databases

BgeeiQ60936.
ExpressionAtlasiQ60936. baseline and differential.
GenevestigatoriQ60936.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Full length sequencing of some human and murine muscular transcript (Telethon Italy project B41)."
    Ievolella C., Millino C., Lanfranchi G.
    Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Skeletal muscle.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Lung and Skin.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Salivary gland.
  4. Wilks J., Billadello J.J.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 250-547 (ISOFORM 1).
    Tissue: Heart.
  5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiADCK3_MOUSE
AccessioniPrimary (citable) accession number: Q60936
Secondary accession number(s): Q8K0M1
, Q9D657, Q9DBR5, Q9JHR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 27, 2003
Last modified: April 29, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.