Reviewed, UniProtKB/Swiss-Prot Q60935 (NAR1_MOUSE)

Names and origin

Protein names

Recommended name:
    GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1
    EC=2.4.2.31
Alternative name(s):
    Mono(ADP-ribosyl)transferase
    YAC-1
    CD_antigen=CD296

Gene names

Name:	Art1
Synonyms:	Art2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine. NADP+ + protein-L-arginine = nicotinamide + N(omega)-((2'-phospho-ADP)-D-ribosyl)-protein-L-arginine.
Subcellular location	Sarcoplasmic reticulum membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Abundantly expressed in cardiac and skeletal muscle. Low levels also found in lung.
Sequence similarities	Belongs to the Arg-specific ADP-ribosyltransferase family.

Ontologies

Keywords
Cellular component	Membrane Sarcoplasmic reticulum
Domain	Signal
Ligand	NAD NADP
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Biological process	protein amino acid ADP-ribosylation Ref.3 Inferred from direct assay. Source: MGI
Cellular component	anchored to membrane Ref.1 Ref.3 Inferred from direct assay. Source: MGI cell surface Ref.1 Inferred from direct assay. Source: MGI sarcoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD(P)+-protein-arginine ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC NAD+ ADP-ribosyltransferase activity Ref.1 Ref.3 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 290	268	GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1		PRO_0000019313
	Propeptide	291 – 325	35	Removed in mature form Potential		PRO_0000019314
Sites
	Active site	242	1	By similarity
	Binding site	117	1	NAD By similarity
	Binding site	174	1	NAD By similarity
	Binding site	228	1	NAD By similarity
Amino acid modifications
	Lipidation	290	1	GPI-anchor amidated serine Potential
	Glycosylation	65	1	N-linked (GlcNAc...) Potential
	Glycosylation	248	1	N-linked (GlcNAc...) Potential
	Disulfide bond	53 ↔ 272		By similarity
	Disulfide bond	169 ↔ 219		By similarity
Experimental info
	Sequence conflict	43	1	P → Q in CAA65094. Ref.2
	Sequence conflict	132	1	R → L in CAA65094. Ref.2
	Sequence conflict	277 – 290	14	Missing in CAA65094. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q60935-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 763EB891F2A49474	FASTA	325	35,898
	10 20 30 40 50 60 MKIPAMMSLL LVSVGLRDGV QVQSYSISQL DIFSQETPLD MAPASFDDQY AGCLADMTAA 70 80 90 100 110 120 LPDLNHSEFQ ANKVYADGWA QANNQWQERR AWGSVWGSLP PSPPGFRDEH GVALLAYTAN 130 140 150 160 170 180 SPLHKEFNAA VREAGRSRAH YLHHFSFKTL HFLLTEALQL LRSHRSRGCQ QVYRGVHGLR 190 200 210 220 230 240 FRPAGPGATV RLGGFASASL KNVAAQQFGE DTFFGIWTCL GAPIRGYSFF PEEEEVLIPP 250 260 270 280 290 300 FETFQVINTS RPTQGPARIY LRALGKRSTY NCEYIKEKKC RSGPCWLGSS APGSISASCS 310 320 LLLLLLFLVL SALPENPGLQ QLTRC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of a glycosylphosphatidylinositol-linked ADP-ribosyltransferase from lymphocytes." Okazaki I.J., Kim H.-J., McElvaney N.G., Lesma E., Moss J. Blood 88:915-921(1996) [PubMed: 8704249] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lymphoma.
[2]	"Assignment of the human and mouse genes for muscle ecto mono (ADPribosyl)transferase to a conserved linkage group on human chromosome 11p15 and mouse chromosome 7." Koch-Nolte F., Kuehl M., Haag F., Cetkovic-Cvrlje M., Leiter E.H., Thiele H.-G. Genomics 36:215-216(1996) [PubMed: 8812442] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[3]	"Molecular cloning of a functional murine arginine-specific mono-ADP-ribosyltransferase and its expression in lymphoid cells." Yu Y., Okamoto S., Nemoto E., Dennert G. DNA Cell Biol. 16:235-244(1997) [PubMed: 9052744] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Limb.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U31510 mRNA. Translation: AAB18628.1. X95825 Genomic DNA. Translation: CAA65094.1. X95842 mRNA. Translation: CAA65099.1. BC063760 mRNA. Translation: AAH63760.1.
IPI	IPI00122552.
RefSeq	NP_033840.2.
UniGene	Mm.261071
3D structure databases
HSSP	HSSP built from PDB template 1GXY based on UniProtKB P20974.
ModBase	Search...
Protein-protein interaction databases
STRING	Q60935.
Proteomic databases
PRIDE	Q60935.
Genome annotation databases
Ensembl	ENSMUST00000033300; ENSMUSP00000033300; ENSMUSG00000030996; Mus musculus. [Genome view]
GeneID	11870.
KEGG	mmu:11870.
UCSC	uc009iqu.1. mouse.
Organism-specific databases
CTD	11870.
MGI	MGI:107511. Art1.
Phylogenomic databases
HOGENOM	Q60935.
HOVERGEN	Q60935.
OMA	YLRALGK.
Enzyme and pathway databases
BRENDA	2.4.2.31. 244.
Gene expression databases
ArrayExpress	Q60935.
Bgee	Q60935.
CleanEx	MM_ART1.
Genevestigator	Q60935.
GermOnline	ENSMUSG00000030996. Mus musculus.
Family and domain databases
InterPro	IPR000768. ART. [Graphical view]
PANTHER	PTHR10339. ART. 1 hit.
Pfam	PF01129. ART. 1 hit. [Graphical view]
PRINTS	PR00970. RIBTRNSFRASE.
PROSITE	PS01291. ART. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	279883.
SOURCE	Search...

Entry information

Entry name

NAR1_MOUSE

Accession

Primary (citable) accession number: Q60935
Secondary accession number(s): P70688

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 3, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)