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Protein

Glutamate receptor ionotropic, kainate 1

Gene

Grik1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei523 – 5231GlutamateBy similarity

GO - Molecular functioni

GO - Biological processi

  • adult behavior Source: MGI
  • behavioral response to pain Source: MGI
  • excitatory postsynaptic potential Source: MGI
  • inhibitory postsynaptic potential Source: MGI
  • ionotropic glutamate receptor signaling pathway Source: MGI
  • membrane depolarization Source: MGI
  • negative regulation of synaptic transmission, glutamatergic Source: MGI
  • positive regulation of gamma-aminobutyric acid secretion Source: MGI
  • positive regulation of synaptic transmission, GABAergic Source: MGI
  • regulation of long-term neuronal synaptic plasticity Source: UniProtKB
  • regulation of membrane potential Source: MGI
  • regulation of short-term neuronal synaptic plasticity Source: UniProtKB
  • synaptic transmission, glutamatergic Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, kainate 1
Short name:
GluK1
Alternative name(s):
Glutamate receptor 5
Short name:
GluR-5
Short name:
GluR5
Gene namesi
Name:Grik1
Synonyms:Glur5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:95814. Grik1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini31 – 561531ExtracellularSequence analysisAdd
BLAST
Transmembranei562 – 58221HelicalSequence analysisAdd
BLAST
Topological domaini583 – 63856CytoplasmicSequence analysisAdd
BLAST
Transmembranei639 – 65921HelicalSequence analysisAdd
BLAST
Topological domaini660 – 72162ExtracellularSequence analysisAdd
BLAST
Transmembranei722 – 74221HelicalSequence analysisAdd
BLAST
Topological domaini743 – 83694CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • dendrite Source: MGI
  • ionotropic glutamate receptor complex Source: MGI
  • kainate selective glutamate receptor complex Source: MGI
  • membrane Source: MGI
  • neuronal postsynaptic density Source: MGI
  • postsynaptic density Source: MGI
  • postsynaptic membrane Source: UniProtKB-SubCell
  • presynaptic membrane Source: MGI
  • synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence analysisAdd
BLAST
Chaini31 – 836806Glutamate receptor ionotropic, kainate 1PRO_0000011542Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi68 – 681N-linked (GlcNAc...)Sequence analysis
Glycosylationi74 – 741N-linked (GlcNAc...)Sequence analysis
Glycosylationi276 – 2761N-linked (GlcNAc...)Sequence analysis
Glycosylationi379 – 3791N-linked (GlcNAc...)Sequence analysis
Glycosylationi413 – 4131N-linked (GlcNAc...)Sequence analysis
Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence analysis
Glycosylationi431 – 4311N-linked (GlcNAc...)Sequence analysis
Glycosylationi546 – 5461N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ60934.
PaxDbiQ60934.
PRIDEiQ60934.

PTM databases

iPTMnetiQ60934.
PhosphoSiteiQ60934.

Expressioni

Tissue specificityi

Most abundant in the cerebellum. Also present in the suprachiasmatic nuclei of the hypothalamus.

Gene expression databases

CleanExiMM_GRIK1.

Interactioni

Subunit structurei

Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers (Probable). The unedited version (Q) assembles into a functional kainate-gated homomeric channel, whereas the edited version (R) is unable to produce channel activity when expressed alone. Both edited and unedited versions can form functional channels with GRIK4 and GRIK5. Interacts with KLHL17 (By similarity).By similarityCurated

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000072107.

Structurei

3D structure databases

ProteinModelPortaliQ60934.
SMRiQ60934. Positions 35-758.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni516 – 5183Glutamate bindingBy similarity
Regioni689 – 6902Glutamate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1054. Eukaryota.
ENOG410XPSH. LUCA.
HOVERGENiHBG051839.
InParanoidiQ60934.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: At least 2 isoforms are produced.

Isoform 1 (identifier: Q60934-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERGTVLIQP GLWTRDTSWT LLYFLCYILP QTSPQVLRIG GIFETVENEP
60 70 80 90 100
VNVEELAFKF AVTSINRNRT LMPNTTLTYD IQRINLFDSF EASRRACDQL
110 120 130 140 150
ALGVAALFGP SHSSSVSAVQ SICNALEVPH IQTRWKHPSV DNRDLFYINL
160 170 180 190 200
YPDYAAISRA VLDLVLYYNW KTVTVVYEDS TGLIRLQELI KAPSRYNIKI
210 220 230 240 250
KIRQLPSGNK DAKPLLKEMK KGKEFYVIFD CSHETAAEIL KQILFMGMMT
260 270 280 290 300
EYYHYFFTTL DLFALDLELY RYSGVNMTGF RLLNIDNPHV SSIIEKWSME
310 320 330 340 350
RLQAPPRPET GLLDGVMTTE AALMYDAVYM VAIASHRASQ LTVSSLQCHR
360 370 380 390 400
HKPWRLGPRF MNLIKEARWD GLTGRITFNK TDGLRKDFDL DIISLKEEGT
410 420 430 440 450
EKIGIWNSNS GLNMTDGNRD RSNNITDSLA NRTLIVTTIL EEPYVMYRKS
460 470 480 490 500
DKPLYGNDRF EGYCLDLLKE LSNILGFLYD VKLVPDGKYG AQNDKGEWNG
510 520 530 540 550
MVKELIDHRA DLAVAPLTIT YVREKVIDFS KPFMTLGISI LYRKPNGTNP
560 570 580 590 600
GVFSFLNPLS PDIWMYVLLA CLGVSCVLFV IARFTPYEWY NPHPCNPDSD
610 620 630 640 650
VVENNFTLLN SFWFGVGALM QQGSELMPKA LSTRIVGGIW WFFTLIIISS
660 670 680 690 700
YTANLAAFLT VERMESPIDS ADDLAKQTKI EYGAVRDGST MTFFKKSKIS
710 720 730 740 750
TYEKMWAFMS SRQPSALGVE NIGGIFIVLA AGLVLSVFVA IGEFIYKSRK
760 770 780 790 800
NNDIEQKGKS SRLRFYFRNK VRFHGSKTES LGVEKCLSFN AIMEELGISL
810 820 830
KNQKKIKKKS RTKGKSSFTS ILTCHQRRTQ RKETVA
Length:836
Mass (Da):95,202
Last modified:July 15, 1998 - v2
Checksum:iA8B6EC4B44FFDBFF
GO

RNA editingi

Edited at position 621.2 Publications
Partially edited.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti621 – 6211Q → R in RNA edited version.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66118 mRNA. No translation available.
U31444 Genomic DNA. Translation: AAA85222.1.
PIRiA41053.
UniGeneiMm.5134.

Genome annotation databases

UCSCiuc007zuw.2. mouse. [Q60934-1]

Keywords - Coding sequence diversityi

Alternative splicing, RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66118 mRNA. No translation available.
U31444 Genomic DNA. Translation: AAA85222.1.
PIRiA41053.
UniGeneiMm.5134.

3D structure databases

ProteinModelPortaliQ60934.
SMRiQ60934. Positions 35-758.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000072107.

PTM databases

iPTMnetiQ60934.
PhosphoSiteiQ60934.

Proteomic databases

MaxQBiQ60934.
PaxDbiQ60934.
PRIDEiQ60934.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc007zuw.2. mouse. [Q60934-1]

Organism-specific databases

MGIiMGI:95814. Grik1.

Phylogenomic databases

eggNOGiKOG1054. Eukaryota.
ENOG410XPSH. LUCA.
HOVERGENiHBG051839.
InParanoidiQ60934.

Miscellaneous databases

PROiQ60934.
SOURCEiSearch...

Gene expression databases

CleanExiMM_GRIK1.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Expression and novel subunit isoforms of glutamate receptor genes GluR5 and GluR6."
    Gregor P., O'Hara B.F., Yang X., Uhl G.R.
    NeuroReport 4:1343-1346(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Brain.
  2. "Q/R site editing in kainate receptor GluR5 and GluR6 pre-mRNAs requires distant intronic sequences."
    Herb A., Higuchi M., Sprengel R., Seeburg P.H.
    Proc. Natl. Acad. Sci. U.S.A. 93:1875-1880(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 584-695, RNA EDITING OF POSITION 621.
    Strain: BALB/cJ.
  3. "RNA editing in brain controls a determinant of ion flow in glutamate-gated channels."
    Sommer B., Koehler M., Sprengel R., Seeburg P.H.
    Cell 67:11-19(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 606-622, RNA EDITING OF POSITION 621.

Entry informationi

Entry nameiGRIK1_MOUSE
AccessioniPrimary (citable) accession number: Q60934
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: June 8, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds domoate > kainate > L-glutamate = quisqualate > CNQX = DNQX > AMPA > dihydrokainate > NMDA (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.