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Protein

Voltage-dependent anion-selective channel protein 1

Gene

Vdac1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.3 Publications

Enzyme regulationi

Inhibited by nitric oxide.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei86Involved in hexokinase bindingBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi255 – 257NADBy similarity3
Nucleotide bindingi273 – 277NADBy similarity5

GO - Molecular functioni

GO - Biological processi

  • anion transport Source: MGI
  • apoptotic process Source: UniProtKB
  • behavioral fear response Source: MGI
  • chemical synaptic transmission Source: MGI
  • epithelial cell differentiation Source: Ensembl
  • learning Source: MGI
  • mitochondrial calcium ion transport Source: Ensembl
  • negative regulation of reactive oxygen species metabolic process Source: ParkinsonsUK-UCL
  • neuron-neuron synaptic transmission Source: MGI
  • regulation of mitophagy Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Apoptosis, Ion transport, Transport

Keywords - Ligandi

NAD, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5205685. Pink/Parkin Mediated Mitophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent anion-selective channel protein 1
Short name:
VDAC-1
Short name:
mVDAC1
Alternative name(s):
Outer mitochondrial membrane protein porin 1
Plasmalemmal porin
Voltage-dependent anion-selective channel protein 5
Short name:
VDAC-5
Short name:
mVDAC5
Gene namesi
Name:Vdac1
Synonyms:Vdac5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:106919. Vdac1.

Subcellular locationi

Isoform Mt-VDAC1 :
Isoform Pl-VDAC1 :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei39 – 46Beta stranded1 Publication8
Transmembranei52 – 61Beta stranded1 Publication10
Transmembranei67 – 77Beta stranded1 PublicationAdd BLAST11
Transmembranei82 – 89Beta stranded1 Publication8
Transmembranei93 – 101Beta stranded1 Publication9
Transmembranei108 – 117Beta stranded1 Publication10
Transmembranei123 – 132Beta stranded1 Publication10
Transmembranei136 – 145Beta stranded1 Publication10
Transmembranei149 – 158Beta stranded1 Publication10
Transmembranei162 – 171Beta stranded1 Publication10
Transmembranei178 – 188Beta stranded1 PublicationAdd BLAST11
Transmembranei191 – 198Beta stranded1 Publication8
Transmembranei202 – 211Beta stranded1 Publication10
Transmembranei215 – 223Beta stranded1 Publication9
Transmembranei230 – 239Beta stranded1 Publication10
Transmembranei244 – 251Beta stranded1 Publication8
Transmembranei255 – 264Beta stranded1 Publication10
Transmembranei268 – 276Beta stranded1 Publication9
Transmembranei286 – 296Beta stranded1 PublicationAdd BLAST11

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • membrane Source: UniProtKB
  • membrane raft Source: UniProtKB-SubCell
  • mitochondrial inner membrane Source: MGI
  • mitochondrial nucleoid Source: MGI
  • mitochondrial outer membrane Source: MGI
  • mitochondrion Source: MGI
  • myelin sheath Source: UniProtKB
  • nucleus Source: MGI
  • plasma membrane Source: UniProtKB
  • pore complex Source: UniProtKB-KW
  • synaptic vesicle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000505002 – 296Voltage-dependent anion-selective channel protein 1Add BLAST295
Isoform Mt-VDAC1 (identifier: Q60932-2)
Initiator methionineiRemovedBy similarity

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylcysteineBy similarity1
Modified residuei14N-acetylmethionine; in isoform Mt-VDAC1By similarity1
Modified residuei26PhosphoserineBy similarity1
Modified residuei32PhosphothreonineCombined sources1
Modified residuei33N6-acetyllysine; alternateCombined sources1
Modified residuei33N6-succinyllysine; alternateCombined sources1
Cross-linki66Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei80PhosphotyrosineCombined sources1
Modified residuei120PhosphothreonineBy similarity1
Modified residuei122N6-acetyllysine; alternateCombined sources1
Cross-linki122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki174Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei206Phosphoserine; by NEK1By similarity1
Modified residuei253PhosphoserineCombined sources1
Modified residuei254PhosphoserineBy similarity1
Modified residuei265N6-acetyllysineCombined sources1
Modified residuei279N6-acetyllysine; alternateBy similarity1
Cross-linki279Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Isoform Mt-VDAC1 (identifier: Q60932-2)
Modified residuei2N-acetylalanine By similarityBy similarity1

Post-translational modificationi

Phosphorylation at Ser-206 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.By similarity
Ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ60932.
PeptideAtlasiQ60932.
PRIDEiQ60932.

2D gel databases

REPRODUCTION-2DPAGEQ60932.
SWISS-2DPAGEQ60932.

PTM databases

iPTMnetiQ60932.
PhosphoSitePlusiQ60932.
SwissPalmiQ60932.

Expressioni

Tissue specificityi

High levels of expression detected in heart, kidney, brain, and skeletal muscle. Not expressed in testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000020402.
CleanExiMM_VDAC1.
ExpressionAtlasiQ60932. baseline and differential.
GenevisibleiQ60932. MM.

Interactioni

Subunit structurei

Interacts with hexokinases including HK1. The HK1-VDAC1 complex interacts with ATF2. Interacts with BCL2L1. Interacts with BAK1. Interacts with BOP/C22orf29 (via BH3 domain) (By similarity). Interacts with the beta amyloid and APP; induces VDAC1 dephosphorylation (PubMed:25168729).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204507. 6 interactors.
IntActiQ60932. 13 interactors.
MINTiMINT-262838.
STRINGi10090.ENSMUSP00000099819.

Structurei

Secondary structure

1296
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni21 – 24Combined sources4
Helixi25 – 32Combined sources8
Beta strandi39 – 45Combined sources7
Beta strandi49 – 61Combined sources13
Turni62 – 64Combined sources3
Beta strandi67 – 77Combined sources11
Turni78 – 81Combined sources4
Beta strandi82 – 89Combined sources8
Beta strandi94 – 105Combined sources12
Beta strandi108 – 116Combined sources9
Turni118 – 120Combined sources3
Beta strandi123 – 133Combined sources11
Beta strandi136 – 145Combined sources10
Beta strandi148 – 159Combined sources12
Beta strandi162 – 171Combined sources10
Turni172 – 175Combined sources4
Beta strandi176 – 187Combined sources12
Beta strandi189 – 198Combined sources10
Turni199 – 201Combined sources3
Beta strandi202 – 212Combined sources11
Beta strandi215 – 224Combined sources10
Beta strandi230 – 241Combined sources12
Beta strandi244 – 251Combined sources8
Beta strandi254 – 265Combined sources12
Beta strandi268 – 277Combined sources10
Helixi278 – 280Combined sources3
Beta strandi282 – 284Combined sources3
Beta strandi287 – 295Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EMNX-ray2.30X14-296[»]
4C69X-ray2.28X14-296[»]
ProteinModelPortaliQ60932.
SMRiQ60932.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60932.

Family & Domainsi

Domaini

Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity (By similarity).By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiKOG3126. Eukaryota.
ENOG410ZBK1. LUCA.
GeneTreeiENSGT00390000011336.
HOGENOMiHOG000188277.
HOVERGENiHBG054036.
InParanoidiQ60932.
KOiK05862.
OMAiYGLMFTE.
OrthoDBiEOG091G0F0O.
PhylomeDBiQ60932.
TreeFamiTF315091.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
IPR030270. VDAC1.
[Graphical view]
PANTHERiPTHR11743:SF13. PTHR11743:SF13. 1 hit.
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Pl-VDAC1 (identifier: Q60932-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCSFFLVLLL WQNMAVPPTY ADLGKSARDV FTKGYGFGLI KLDLKTKSEN
60 70 80 90 100
GLEFTSSGSA NTETTKVNGS LETKYRWTEY GLTFTEKWNT DNTLGTEITV
110 120 130 140 150
EDQLARGLKL TFDSSFSPNT GKKNAKIKTG YKREHINLGC DVDFDIAGPS
160 170 180 190 200
IRGALVLGYE GWLAGYQMNF ETSKSRVTQS NFAVGYKTDE FQLHTNVNDG
210 220 230 240 250
TEFGGSIYQK VNKKLETAVN LAWTAGNSNT RFGIAAKYQV DPDACFSAKV
260 270 280 290
NNSSLIGLGY TQTLKPGIKL TLSALLDGKN VNAGGHKLGL GLEFQA
Length:296
Mass (Da):32,351
Last modified:December 1, 2000 - v3
Checksum:iC0710C1717063B32
GO
Isoform Mt-VDAC1 (identifier: Q60932-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.

Show »
Length:283
Mass (Da):30,756
Checksum:iD32B05B8A8D75732
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0050751 – 13Missing in isoform Mt-VDAC1. 4 PublicationsAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30840 mRNA. Translation: AAB47777.1.
AK168672 mRNA. Translation: BAE40522.1.
AK169671 mRNA. Translation: BAE41292.1.
AK169354 mRNA. Translation: BAE41103.1.
AK169282 mRNA. Translation: BAE41040.1.
AK169160 mRNA. Translation: BAE40939.1.
AL645589 Genomic DNA. Translation: CAI24939.1.
AL645589 Genomic DNA. Translation: CAM19812.1.
BC092257 mRNA. Translation: AAH92257.1.
CCDSiCCDS24671.1. [Q60932-2]
RefSeqiNP_035824.1. NM_011694.5. [Q60932-2]
UniGeneiMm.3555.
Mm.470023.

Genome annotation databases

EnsembliENSMUST00000020673; ENSMUSP00000020673; ENSMUSG00000020402. [Q60932-1]
ENSMUST00000102758; ENSMUSP00000099819; ENSMUSG00000020402. [Q60932-2]
GeneIDi22333.
KEGGimmu:22333.
UCSCiuc007ivm.1. mouse. [Q60932-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30840 mRNA. Translation: AAB47777.1.
AK168672 mRNA. Translation: BAE40522.1.
AK169671 mRNA. Translation: BAE41292.1.
AK169354 mRNA. Translation: BAE41103.1.
AK169282 mRNA. Translation: BAE41040.1.
AK169160 mRNA. Translation: BAE40939.1.
AL645589 Genomic DNA. Translation: CAI24939.1.
AL645589 Genomic DNA. Translation: CAM19812.1.
BC092257 mRNA. Translation: AAH92257.1.
CCDSiCCDS24671.1. [Q60932-2]
RefSeqiNP_035824.1. NM_011694.5. [Q60932-2]
UniGeneiMm.3555.
Mm.470023.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EMNX-ray2.30X14-296[»]
4C69X-ray2.28X14-296[»]
ProteinModelPortaliQ60932.
SMRiQ60932.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204507. 6 interactors.
IntActiQ60932. 13 interactors.
MINTiMINT-262838.
STRINGi10090.ENSMUSP00000099819.

PTM databases

iPTMnetiQ60932.
PhosphoSitePlusiQ60932.
SwissPalmiQ60932.

2D gel databases

REPRODUCTION-2DPAGEQ60932.
SWISS-2DPAGEQ60932.

Proteomic databases

PaxDbiQ60932.
PeptideAtlasiQ60932.
PRIDEiQ60932.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020673; ENSMUSP00000020673; ENSMUSG00000020402. [Q60932-1]
ENSMUST00000102758; ENSMUSP00000099819; ENSMUSG00000020402. [Q60932-2]
GeneIDi22333.
KEGGimmu:22333.
UCSCiuc007ivm.1. mouse. [Q60932-1]

Organism-specific databases

CTDi7416.
MGIiMGI:106919. Vdac1.

Phylogenomic databases

eggNOGiKOG3126. Eukaryota.
ENOG410ZBK1. LUCA.
GeneTreeiENSGT00390000011336.
HOGENOMiHOG000188277.
HOVERGENiHBG054036.
InParanoidiQ60932.
KOiK05862.
OMAiYGLMFTE.
OrthoDBiEOG091G0F0O.
PhylomeDBiQ60932.
TreeFamiTF315091.

Enzyme and pathway databases

ReactomeiR-MMU-5205685. Pink/Parkin Mediated Mitophagy.

Miscellaneous databases

ChiTaRSiVdac1. mouse.
EvolutionaryTraceiQ60932.
PROiQ60932.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020402.
CleanExiMM_VDAC1.
ExpressionAtlasiQ60932. baseline and differential.
GenevisibleiQ60932. MM.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
IPR030270. VDAC1.
[Graphical view]
PANTHERiPTHR11743:SF13. PTHR11743:SF13. 1 hit.
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVDAC1_MOUSE
AccessioniPrimary (citable) accession number: Q60932
Secondary accession number(s): B1ASZ9, Q5SVC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.