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Protein

Voltage-dependent anion-selective channel protein 2

Gene

Vdac2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei85Involved in hexokinase bindingBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi254 – 256NADBy similarity3
Nucleotide bindingi272 – 276NADBy similarity5

GO - Molecular functioni

GO - Biological processi

  • anion transport Source: MGI
  • negative regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
  • negative regulation of protein polymerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

NAD, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent anion-selective channel protein 2
Short name:
VDAC-2
Short name:
mVDAC2
Alternative name(s):
Outer mitochondrial membrane protein porin 2
Voltage-dependent anion-selective channel protein 6
Short name:
VDAC-6
Short name:
mVDAC6
Gene namesi
Name:Vdac2
Synonyms:Vdac6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:106915. Vdac2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei38 – 47Beta strandedBy similarity10
Transmembranei51 – 59Beta strandedBy similarity9
Transmembranei66 – 76Beta strandedBy similarityAdd BLAST11
Transmembranei81 – 88Beta strandedBy similarity8
Transmembranei92 – 101Beta strandedBy similarity10
Transmembranei107 – 116Beta strandedBy similarity10
Transmembranei123 – 132Beta strandedBy similarity10
Transmembranei135 – 142Beta strandedBy similarity8
Transmembranei149 – 157Beta strandedBy similarity9
Transmembranei162 – 170Beta strandedBy similarity9
Transmembranei175 – 187Beta strandedBy similarityAdd BLAST13
Transmembranei190 – 197Beta strandedBy similarity8
Transmembranei201 – 210Beta strandedBy similarity10
Transmembranei214 – 223Beta strandedBy similarity10
Transmembranei230 – 239Beta strandedBy similarity10
Transmembranei243 – 250Beta strandedBy similarity8
Transmembranei254 – 263Beta strandedBy similarity10
Transmembranei266 – 275Beta strandedBy similarity10
Transmembranei285 – 294Beta strandedBy similarity10

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • mitochondrial inner membrane Source: MGI
  • mitochondrial nucleoid Source: MGI
  • mitochondrial outer membrane Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
  • myelin sheath Source: UniProtKB
  • nucleus Source: MGI
  • pore complex Source: UniProtKB-KW
  • synaptic vesicle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000505061 – 295Voltage-dependent anion-selective channel protein 2Add BLAST295

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32N6-acetyllysine; alternateCombined sources1
Modified residuei32N6-succinyllysine; alternateCombined sources1
Cross-linki32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki65Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei121N6-acetyllysine; alternateCombined sources1
Cross-linki121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki278Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ60930.
MaxQBiQ60930.
PaxDbiQ60930.
PeptideAtlasiQ60930.
PRIDEiQ60930.

2D gel databases

REPRODUCTION-2DPAGEQ60930.

PTM databases

iPTMnetiQ60930.
PhosphoSitePlusiQ60930.
SwissPalmiQ60930.

Expressioni

Tissue specificityi

Highest levels of expression detected in testis, less but still abundant expression in heart, kidney, brain, and skeletal muscle.

Gene expression databases

BgeeiENSMUSG00000021771.
CleanExiMM_VDAC2.
ExpressionAtlasiQ60930. baseline and differential.
GenevisibleiQ60930. MM.

Interactioni

Subunit structurei

Interacts with hexokinases.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Bak1O087343EBI-444578,EBI-822441

Protein-protein interaction databases

BioGridi204508. 5 interactors.
IntActiQ60930. 18 interactors.
MINTiMINT-1839157.
STRINGi10090.ENSMUSP00000022293.

Structurei

3D structure databases

ProteinModelPortaliQ60930.
SMRiQ60930.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiKOG3126. Eukaryota.
ENOG410ZBK1. LUCA.
GeneTreeiENSGT00390000011336.
HOGENOMiHOG000188277.
HOVERGENiHBG054036.
InParanoidiQ60930.
KOiK15040.
OMAiMCVPPSY.
OrthoDBiEOG091G0F0O.
PhylomeDBiQ60930.
TreeFamiTF315091.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
IPR030277. VDAC2.
[Graphical view]
PANTHERiPTHR11743:SF12. PTHR11743:SF12. 1 hit.
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60930-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAECCVPVCP RPMCIPPPYA DLGKAARDIF NKGFGFGLVK LDVKTKSCSG
60 70 80 90 100
VEFSTSGSSN TDTGKVSGTL ETKYKWCEYG LTFTEKWNTD NTLGTEIAIE
110 120 130 140 150
DQICQGLKLT FDTTFSPNTG KKSGKIKSAY KRECINLGCD VDFDFAGPAI
160 170 180 190 200
HGSAVFGYEG WLAGYQMTFD SAKSKLTRSN FAVGYRTGDF QLHTNVNNGT
210 220 230 240 250
EFGGSIYQKV CEDFDTSVNL AWTSGTNCTR FGIAAKYQLD PTASISAKVN
260 270 280 290
NSSLIGVGYT QTLRPGVKLT LSALVDGKSF NAGGHKLGLA LELEA
Length:295
Mass (Da):31,733
Last modified:August 1, 1998 - v2
Checksum:i7BBCDC625AD013C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti260T → A in AAH03731 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30838 mRNA. Translation: AAC13321.1.
AK168199 mRNA. Translation: BAE40159.1.
AK159561 mRNA. Translation: BAE35185.1.
AK152274 mRNA. Translation: BAE31090.1.
AK150940 mRNA. Translation: BAE29975.1.
AK150627 mRNA. Translation: BAE29717.1.
AK150504 mRNA. Translation: BAE29617.1.
AK012359 mRNA. Translation: BAE43231.1.
AY294423 Genomic DNA. Translation: AAQ01516.1.
BC003731 mRNA. Translation: AAH03731.2.
CCDSiCCDS26867.1.
RefSeqiNP_035825.1. NM_011695.2.
UniGeneiMm.262327.

Genome annotation databases

EnsembliENSMUST00000022293; ENSMUSP00000022293; ENSMUSG00000021771.
GeneIDi22334.
KEGGimmu:22334.
UCSCiuc007sls.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30838 mRNA. Translation: AAC13321.1.
AK168199 mRNA. Translation: BAE40159.1.
AK159561 mRNA. Translation: BAE35185.1.
AK152274 mRNA. Translation: BAE31090.1.
AK150940 mRNA. Translation: BAE29975.1.
AK150627 mRNA. Translation: BAE29717.1.
AK150504 mRNA. Translation: BAE29617.1.
AK012359 mRNA. Translation: BAE43231.1.
AY294423 Genomic DNA. Translation: AAQ01516.1.
BC003731 mRNA. Translation: AAH03731.2.
CCDSiCCDS26867.1.
RefSeqiNP_035825.1. NM_011695.2.
UniGeneiMm.262327.

3D structure databases

ProteinModelPortaliQ60930.
SMRiQ60930.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204508. 5 interactors.
IntActiQ60930. 18 interactors.
MINTiMINT-1839157.
STRINGi10090.ENSMUSP00000022293.

PTM databases

iPTMnetiQ60930.
PhosphoSitePlusiQ60930.
SwissPalmiQ60930.

2D gel databases

REPRODUCTION-2DPAGEQ60930.

Proteomic databases

EPDiQ60930.
MaxQBiQ60930.
PaxDbiQ60930.
PeptideAtlasiQ60930.
PRIDEiQ60930.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022293; ENSMUSP00000022293; ENSMUSG00000021771.
GeneIDi22334.
KEGGimmu:22334.
UCSCiuc007sls.1. mouse.

Organism-specific databases

CTDi7417.
MGIiMGI:106915. Vdac2.

Phylogenomic databases

eggNOGiKOG3126. Eukaryota.
ENOG410ZBK1. LUCA.
GeneTreeiENSGT00390000011336.
HOGENOMiHOG000188277.
HOVERGENiHBG054036.
InParanoidiQ60930.
KOiK15040.
OMAiMCVPPSY.
OrthoDBiEOG091G0F0O.
PhylomeDBiQ60930.
TreeFamiTF315091.

Miscellaneous databases

PROiQ60930.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021771.
CleanExiMM_VDAC2.
ExpressionAtlasiQ60930. baseline and differential.
GenevisibleiQ60930. MM.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
IPR030277. VDAC2.
[Graphical view]
PANTHERiPTHR11743:SF12. PTHR11743:SF12. 1 hit.
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVDAC2_MOUSE
AccessioniPrimary (citable) accession number: Q60930
Secondary accession number(s): Q78MH6, Q99L98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.