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Protein

Voltage-dependent anion-selective channel protein 2

Gene

Vdac2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei85 – 851Involved in hexokinase bindingBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi254 – 2563NADBy similarity
Nucleotide bindingi272 – 2765NADBy similarity

GO - Molecular functioni

GO - Biological processi

  • anion transport Source: MGI
  • negative regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
  • negative regulation of protein polymerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

NAD, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent anion-selective channel protein 2
Short name:
VDAC-2
Short name:
mVDAC2
Alternative name(s):
Outer mitochondrial membrane protein porin 2
Voltage-dependent anion-selective channel protein 6
Short name:
VDAC-6
Short name:
mVDAC6
Gene namesi
Name:Vdac2
Synonyms:Vdac6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:106915. Vdac2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei38 – 4710Beta strandedBy similarity
Transmembranei51 – 599Beta strandedBy similarity
Transmembranei66 – 7611Beta strandedBy similarityAdd
BLAST
Transmembranei81 – 888Beta strandedBy similarity
Transmembranei92 – 10110Beta strandedBy similarity
Transmembranei107 – 11610Beta strandedBy similarity
Transmembranei123 – 13210Beta strandedBy similarity
Transmembranei135 – 1428Beta strandedBy similarity
Transmembranei149 – 1579Beta strandedBy similarity
Transmembranei162 – 1709Beta strandedBy similarity
Transmembranei175 – 18713Beta strandedBy similarityAdd
BLAST
Transmembranei190 – 1978Beta strandedBy similarity
Transmembranei201 – 21010Beta strandedBy similarity
Transmembranei214 – 22310Beta strandedBy similarity
Transmembranei230 – 23910Beta strandedBy similarity
Transmembranei243 – 2508Beta strandedBy similarity
Transmembranei254 – 26310Beta strandedBy similarity
Transmembranei266 – 27510Beta strandedBy similarity
Transmembranei285 – 29410Beta strandedBy similarity

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • membrane raft Source: MGI
  • mitochondrial inner membrane Source: MGI
  • mitochondrial nucleoid Source: MGI
  • mitochondrial outer membrane Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
  • myelin sheath Source: UniProtKB
  • nucleus Source: MGI
  • pore complex Source: UniProtKB-KW
  • synaptic vesicle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295Voltage-dependent anion-selective channel protein 2PRO_0000050506Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321N6-acetyllysine; alternateCombined sources
Modified residuei32 – 321N6-succinyllysine; alternateCombined sources
Cross-linki32 – 32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki65 – 65Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei121 – 1211N6-acetyllysine; alternateCombined sources
Cross-linki121 – 121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki122 – 122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki278 – 278Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ60930.
MaxQBiQ60930.
PaxDbiQ60930.
PeptideAtlasiQ60930.
PRIDEiQ60930.

2D gel databases

REPRODUCTION-2DPAGEQ60930.

PTM databases

iPTMnetiQ60930.
PhosphoSiteiQ60930.
SwissPalmiQ60930.

Expressioni

Tissue specificityi

Highest levels of expression detected in testis, less but still abundant expression in heart, kidney, brain, and skeletal muscle.

Gene expression databases

BgeeiENSMUSG00000021771.
CleanExiMM_VDAC2.
ExpressionAtlasiQ60930. baseline and differential.
GenevisibleiQ60930. MM.

Interactioni

Subunit structurei

Interacts with hexokinases.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Bak1O087343EBI-444578,EBI-822441

Protein-protein interaction databases

BioGridi204508. 5 interactions.
IntActiQ60930. 16 interactions.
MINTiMINT-1839157.
STRINGi10090.ENSMUSP00000022293.

Structurei

3D structure databases

ProteinModelPortaliQ60930.
SMRiQ60930. Positions 13-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiKOG3126. Eukaryota.
ENOG410ZBK1. LUCA.
GeneTreeiENSGT00390000011336.
HOGENOMiHOG000188277.
HOVERGENiHBG054036.
InParanoidiQ60930.
KOiK15040.
OMAiMCVPPSY.
OrthoDBiEOG091G0F0O.
PhylomeDBiQ60930.
TreeFamiTF315091.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
IPR030277. VDAC2.
[Graphical view]
PANTHERiPTHR11743:SF12. PTHR11743:SF12. 1 hit.
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60930-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAECCVPVCP RPMCIPPPYA DLGKAARDIF NKGFGFGLVK LDVKTKSCSG
60 70 80 90 100
VEFSTSGSSN TDTGKVSGTL ETKYKWCEYG LTFTEKWNTD NTLGTEIAIE
110 120 130 140 150
DQICQGLKLT FDTTFSPNTG KKSGKIKSAY KRECINLGCD VDFDFAGPAI
160 170 180 190 200
HGSAVFGYEG WLAGYQMTFD SAKSKLTRSN FAVGYRTGDF QLHTNVNNGT
210 220 230 240 250
EFGGSIYQKV CEDFDTSVNL AWTSGTNCTR FGIAAKYQLD PTASISAKVN
260 270 280 290
NSSLIGVGYT QTLRPGVKLT LSALVDGKSF NAGGHKLGLA LELEA
Length:295
Mass (Da):31,733
Last modified:August 1, 1998 - v2
Checksum:i7BBCDC625AD013C7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti260 – 2601T → A in AAH03731 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30838 mRNA. Translation: AAC13321.1.
AK168199 mRNA. Translation: BAE40159.1.
AK159561 mRNA. Translation: BAE35185.1.
AK152274 mRNA. Translation: BAE31090.1.
AK150940 mRNA. Translation: BAE29975.1.
AK150627 mRNA. Translation: BAE29717.1.
AK150504 mRNA. Translation: BAE29617.1.
AK012359 mRNA. Translation: BAE43231.1.
AY294423 Genomic DNA. Translation: AAQ01516.1.
BC003731 mRNA. Translation: AAH03731.2.
CCDSiCCDS26867.1.
RefSeqiNP_035825.1. NM_011695.2.
UniGeneiMm.262327.

Genome annotation databases

EnsembliENSMUST00000022293; ENSMUSP00000022293; ENSMUSG00000021771.
GeneIDi22334.
KEGGimmu:22334.
UCSCiuc007sls.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30838 mRNA. Translation: AAC13321.1.
AK168199 mRNA. Translation: BAE40159.1.
AK159561 mRNA. Translation: BAE35185.1.
AK152274 mRNA. Translation: BAE31090.1.
AK150940 mRNA. Translation: BAE29975.1.
AK150627 mRNA. Translation: BAE29717.1.
AK150504 mRNA. Translation: BAE29617.1.
AK012359 mRNA. Translation: BAE43231.1.
AY294423 Genomic DNA. Translation: AAQ01516.1.
BC003731 mRNA. Translation: AAH03731.2.
CCDSiCCDS26867.1.
RefSeqiNP_035825.1. NM_011695.2.
UniGeneiMm.262327.

3D structure databases

ProteinModelPortaliQ60930.
SMRiQ60930. Positions 13-295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204508. 5 interactions.
IntActiQ60930. 16 interactions.
MINTiMINT-1839157.
STRINGi10090.ENSMUSP00000022293.

PTM databases

iPTMnetiQ60930.
PhosphoSiteiQ60930.
SwissPalmiQ60930.

2D gel databases

REPRODUCTION-2DPAGEQ60930.

Proteomic databases

EPDiQ60930.
MaxQBiQ60930.
PaxDbiQ60930.
PeptideAtlasiQ60930.
PRIDEiQ60930.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022293; ENSMUSP00000022293; ENSMUSG00000021771.
GeneIDi22334.
KEGGimmu:22334.
UCSCiuc007sls.1. mouse.

Organism-specific databases

CTDi7417.
MGIiMGI:106915. Vdac2.

Phylogenomic databases

eggNOGiKOG3126. Eukaryota.
ENOG410ZBK1. LUCA.
GeneTreeiENSGT00390000011336.
HOGENOMiHOG000188277.
HOVERGENiHBG054036.
InParanoidiQ60930.
KOiK15040.
OMAiMCVPPSY.
OrthoDBiEOG091G0F0O.
PhylomeDBiQ60930.
TreeFamiTF315091.

Miscellaneous databases

PROiQ60930.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021771.
CleanExiMM_VDAC2.
ExpressionAtlasiQ60930. baseline and differential.
GenevisibleiQ60930. MM.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
IPR030277. VDAC2.
[Graphical view]
PANTHERiPTHR11743:SF12. PTHR11743:SF12. 1 hit.
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVDAC2_MOUSE
AccessioniPrimary (citable) accession number: Q60930
Secondary accession number(s): Q78MH6, Q99L98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.