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Protein

Gamma-glutamyltranspeptidase 1

Gene

Ggt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates, and other gamma-glutamyl compounds. The metabolism of glutathione releases free glutamate and the dipeptide, cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Indirectly regulates multiple aspects of skeletal biology.1 Publication

Catalytic activityi

A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.By similarity
Glutathione + H2O = L-cysteinylglycine + L-glutamate.By similarity
Leukotriene C4 + H2O = leukotriene D4 + L-glutamate.By similarity

Enzyme regulationi

Activated by autocatalytic cleavage.By similarity

Pathwayi: glutathione metabolism

This protein is involved in the pathway glutathione metabolism, which is part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the pathway glutathione metabolism and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei106GlutamateBy similarity1
Active sitei380NucleophileBy similarity1
Binding sitei398GlutamateBy similarity1
Binding sitei419GlutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to mechanical stimulus Source: UniProtKB
  • cysteine biosynthetic process Source: UniProtKB
  • glutamate metabolic process Source: UniProtKB
  • glutathione biosynthetic process Source: UniProtKB
  • glutathione catabolic process Source: UniProtKB
  • glutathione metabolic process Source: MGI
  • leukotriene biosynthetic process Source: MGI
  • lung connective tissue development Source: UniProtKB
  • regulation of immune system process Source: UniProtKB
  • regulation of inflammatory response Source: UniProtKB
  • spermatogenesis Source: MGI
  • zymogen activation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Hydrolase, Protease, Transferase

Keywords - Biological processi

Glutathione biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-174403. Glutathione synthesis and recycling.
R-MMU-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-MMU-5423646. Aflatoxin activation and detoxification.
UniPathwayiUPA00204.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyltranspeptidase 1 (EC:2.3.2.2By similarity)
Short name:
GGT 1
Alternative name(s):
Gamma-glutamyltransferase 1
Glutathione hydrolase 1 (EC:3.4.19.13By similarity)
Leukotriene-C4 hydrolase (EC:3.4.19.14By similarity)
CD_antigen: CD224
Cleaved into the following 2 chains:
Gene namesi
Name:Ggt1
Synonyms:Ggt, Ggtp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:95706. Ggt1.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type II membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 26Helical; Signal-anchor for type II membrane proteinCuratedAdd BLAST22
Topological domaini27 – 568ExtracellularSequence analysisAdd BLAST542

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • integral component of plasma membrane Source: MGI
  • membrane-bounded vesicle Source: MGI
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Ggt1 are a cause of glutathionuria, severe growth failure, reduced life spans and infertility. Ggt1-deficient mice have multiple metabolic abnormalities and are dwarf. Some abnormalities can be ameliorated by N-acetylcysteine treatment.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000110601 – 379Gamma-glutamyltranspeptidase 1 heavy chainAdd BLAST379
ChainiPRO_0000011061380 – 568Gamma-glutamyltranspeptidase 1 light chainAdd BLAST189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi49 ↔ 73By similarity
Glycosylationi94N-linked (GlcNAc...)Sequence analysis1
Glycosylationi114N-linked (GlcNAc...)1 Publication1
Glycosylationi119N-linked (GlcNAc...)Sequence analysis1
Glycosylationi120N-linked (GlcNAc...); atypical1 Publication1
Disulfide bondi191 ↔ 195By similarity
Glycosylationi229N-linked (GlcNAc...)1 Publication1
Glycosylationi343N-linked (GlcNAc...)Sequence analysis1
Glycosylationi510N-linked (GlcNAc...)1 Publication1

Post-translational modificationi

N-glycosylated on both chains.By similarity
Cleaved by autocatalysis into a large and a small subunit and the autocatalytic cleavage is essential to the functional activation of the enzyme.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ60928.
PaxDbiQ60928.
PRIDEiQ60928.
TopDownProteomicsiQ60928.

PTM databases

iPTMnetiQ60928.
PhosphoSitePlusiQ60928.

Expressioni

Gene expression databases

BgeeiENSMUSG00000006345.
CleanExiMM_GGT1.
ExpressionAtlasiQ60928. baseline and differential.
GenevisibleiQ60928. MM.

Interactioni

Subunit structurei

Heterodimer composed of the light and heavy chains. The active site is located in the light chain.By similarity

Protein-protein interaction databases

IntActiQ60928. 2 interactors.
MINTiMINT-4096056.
STRINGi10090.ENSMUSP00000006508.

Structurei

3D structure databases

ProteinModelPortaliQ60928.
SMRiQ60928.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni450 – 451Glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the gamma-glutamyltransferase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2410. Eukaryota.
COG0405. LUCA.
GeneTreeiENSGT00550000074591.
HOGENOMiHOG000175620.
HOVERGENiHBG005835.
InParanoidiQ60928.
KOiK18592.
OMAiSTSHFVI.
OrthoDBiEOG091G03Y3.
PhylomeDBiQ60928.
TreeFamiTF313608.

Family and domain databases

InterProiIPR000101. GGT_peptidase.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PANTHERiPTHR11686. PTHR11686. 1 hit.
PfamiPF01019. G_glu_transpept. 1 hit.
[Graphical view]
PRINTSiPR01210. GGTRANSPTASE.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR00066. g_glut_trans. 1 hit.
PROSITEiPS00462. G_GLU_TRANSPEPTIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q60928-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNRFLVLGL VAVVLVFVII GLCIWLPYTS GKPDHVYSRA AVATDAKRCS
60 70 80 90 100
EIGRDILQEG GSVVDAAIAS LLCMGLMNAH SMGIGGGLFF TIYNSTTGKV
110 120 130 140 150
EVINAREVAP RLANTTMFNN SKDSEEGGLS VAVPGEIRGY ELAHQRHGRL
160 170 180 190 200
PWARLFQPSI QLARHGFPVG KGLAIALDKK RDVIEKTPAL CEVFCRQGKV
210 220 230 240 250
LQEGETVTMP KLADTLQILA QEGAKAFYNG SLTAQIVKDI QEAGGIMTVE
260 270 280 290 300
DLNNYRAELI EHPMSIGLGD ATLYVPSAPL SGPVLILILN ILKGYNFSPK
310 320 330 340 350
SVATPEQKAL TYHRIVEAFR FAYAKRTMLG DPKFVDVSQV IRNMSSEFYA
360 370 380 390 400
TQLRARITDE TTHPAAYYEP EFYLQDDGGT AHLSAVSEDG SAVAATSTIN
410 420 430 440 450
LYFGSKVLSR VSGILFNDEM DDFSSPNFIN QFRVAPSPAN FIKPGKQPLS
460 470 480 490 500
SMCPSIILDK DGQVRMVVGA SGGTQITTSV ALAIINSLWF GYDVKRAVEE
510 520 530 540 550
PRLHNQLLPN TTTVEKDIDQ VVTAGLKIRH HHTEVTPTFI AVVQAVVRAS
560
GGWAAASDSR KGGEPAGY
Length:568
Mass (Da):61,563
Last modified:November 1, 1996 - v1
Checksum:i293C6E224FDC0766
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30509 mRNA. Translation: AAA97395.1.
BC012969 mRNA. Translation: AAH12969.1.
CCDSiCCDS23927.1.
PIRiJC4570.
RefSeqiNP_001292921.1. NM_001305992.1.
NP_032142.1. NM_008116.3.
XP_006513293.1. XM_006513230.3.
XP_006513294.1. XM_006513231.3.
UniGeneiMm.4559.
Mm.486584.

Genome annotation databases

EnsembliENSMUST00000006508; ENSMUSP00000006508; ENSMUSG00000006345.
ENSMUST00000134503; ENSMUSP00000121312; ENSMUSG00000006345.
GeneIDi14598.
KEGGimmu:14598.
UCSCiuc007fqo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30509 mRNA. Translation: AAA97395.1.
BC012969 mRNA. Translation: AAH12969.1.
CCDSiCCDS23927.1.
PIRiJC4570.
RefSeqiNP_001292921.1. NM_001305992.1.
NP_032142.1. NM_008116.3.
XP_006513293.1. XM_006513230.3.
XP_006513294.1. XM_006513231.3.
UniGeneiMm.4559.
Mm.486584.

3D structure databases

ProteinModelPortaliQ60928.
SMRiQ60928.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ60928. 2 interactors.
MINTiMINT-4096056.
STRINGi10090.ENSMUSP00000006508.

PTM databases

iPTMnetiQ60928.
PhosphoSitePlusiQ60928.

Proteomic databases

MaxQBiQ60928.
PaxDbiQ60928.
PRIDEiQ60928.
TopDownProteomicsiQ60928.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006508; ENSMUSP00000006508; ENSMUSG00000006345.
ENSMUST00000134503; ENSMUSP00000121312; ENSMUSG00000006345.
GeneIDi14598.
KEGGimmu:14598.
UCSCiuc007fqo.2. mouse.

Organism-specific databases

CTDi2678.
MGIiMGI:95706. Ggt1.

Phylogenomic databases

eggNOGiKOG2410. Eukaryota.
COG0405. LUCA.
GeneTreeiENSGT00550000074591.
HOGENOMiHOG000175620.
HOVERGENiHBG005835.
InParanoidiQ60928.
KOiK18592.
OMAiSTSHFVI.
OrthoDBiEOG091G03Y3.
PhylomeDBiQ60928.
TreeFamiTF313608.

Enzyme and pathway databases

UniPathwayiUPA00204.
ReactomeiR-MMU-174403. Glutathione synthesis and recycling.
R-MMU-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-MMU-5423646. Aflatoxin activation and detoxification.

Miscellaneous databases

ChiTaRSiGgt1. mouse.
PROiQ60928.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006345.
CleanExiMM_GGT1.
ExpressionAtlasiQ60928. baseline and differential.
GenevisibleiQ60928. MM.

Family and domain databases

InterProiIPR000101. GGT_peptidase.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PANTHERiPTHR11686. PTHR11686. 1 hit.
PfamiPF01019. G_glu_transpept. 1 hit.
[Graphical view]
PRINTSiPR01210. GGTRANSPTASE.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR00066. g_glut_trans. 1 hit.
PROSITEiPS00462. G_GLU_TRANSPEPTIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGGT1_MOUSE
AccessioniPrimary (citable) accession number: Q60928
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.