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Protein

D site-binding protein

Gene

Dbp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • circadian rhythm Source: MGI
  • liver development Source: Ensembl
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_115781. Bmal1:Clock,Npas2 activates circadian gene expression.
REACT_344843. BMAL1:CLOCK,NPAS2 activates circadian gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
D site-binding protein
Alternative name(s):
Albumin D box-binding protein
Albumin D-element-binding protein
Gene namesi
Name:Dbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:94866. Dbp.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325D site-binding proteinPRO_0000076508Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei86 – 861PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ60925.

PTM databases

PhosphoSiteiQ60925.

Expressioni

Tissue specificityi

Expressed in the suprachiasmatic nuclei (SCN) and in most peripheral tissues, with a strong circadian rhythmicity.

Inductioni

Accumulates according to a robust circadian rhythm in liver and kidney. In liver nuclei, the amplitude of daily oscillation has been estimated to be >50-fold, and 2-fold in the brain.

Gene expression databases

BgeeiQ60925.
CleanExiMM_DBP.
ExpressionAtlasiQ60925. baseline and differential.
GenevisibleiQ60925. MM.

Interactioni

Subunit structurei

Binds DNA as a homodimer or a heterodimer. Can form a heterodimer with TEF (By similarity).By similarity

Protein-protein interaction databases

BioGridi199060. 7 interactions.
STRINGi10090.ENSMUSP00000079693.

Structurei

3D structure databases

ProteinModelPortaliQ60925.
SMRiQ60925. Positions 229-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini255 – 31864bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni257 – 27923Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni283 – 29715Leucine-zipperPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi129 – 1357Poly-Pro
Compositional biasi156 – 1616Poly-Ser
Compositional biasi188 – 23952Pro-rich (proline/acidic region (PAR))Add
BLAST

Sequence similaritiesi

Belongs to the bZIP family. PAR subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG238019.
GeneTreeiENSGT00440000039070.
HOGENOMiHOG000234383.
HOVERGENiHBG004983.
InParanoidiQ60925.
KOiK09056.
OMAiKEPTSCL.
OrthoDBiEOG712TZJ.
PhylomeDBiQ60925.
TreeFamiTF315869.

Family and domain databases

InterProiIPR004827. bZIP.
IPR029830. DBP.
[Graphical view]
PANTHERiPTHR11988:SF7. PTHR11988:SF7. 1 hit.
PfamiPF07716. bZIP_2. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60925-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARPLSDRTP GPLLLGGPAG APPGGGALLG LRSLLQGNSK PKEPASCLLK
60 70 80 90 100
EKERKATLPS APVPGPGLET AGPADAPSGA VSGGGSPRGR SGPVAGPSLF
110 120 130 140 150
APLLWERTLP FGDVEYVDLD AFLLEHGLPP SPPPPGGLSP APSPARTPAP
160 170 180 190 200
SPGPGSCSSS SPRSSPGHAP ARATLGAAGG HRAGLTSRDT PSPVDPDTVE
210 220 230 240 250
VLMTFEPDPA DLALSSIPGH ETFDPRRHRF SEEELKPQPI MKKARKVQVP
260 270 280 290 300
EEQKDEKYWS RRYKNNEAAK RSRDARRLKE NQISVRAAFL EKENALLRQE
310 320
VVAVRQELSH YRAVLSRYQA QHGTL
Length:325
Mass (Da):34,380
Last modified:May 10, 2004 - v2
Checksum:iB2B2A3E091845A16
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601S → T in AAA73924 (Ref. 1) Curated
Sequence conflicti109 – 1091L → F in AAA73924 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29762 Genomic DNA. Translation: AAA73924.1.
BC018323 mRNA. Translation: AAH18323.1.
CCDSiCCDS21260.1.
RefSeqiNP_058670.2. NM_016974.3.
UniGeneiMm.24222.
Mm.378235.

Genome annotation databases

EnsembliENSMUST00000080885; ENSMUSP00000079693; ENSMUSG00000059824.
GeneIDi13170.
KEGGimmu:13170.
UCSCiuc009gws.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29762 Genomic DNA. Translation: AAA73924.1.
BC018323 mRNA. Translation: AAH18323.1.
CCDSiCCDS21260.1.
RefSeqiNP_058670.2. NM_016974.3.
UniGeneiMm.24222.
Mm.378235.

3D structure databases

ProteinModelPortaliQ60925.
SMRiQ60925. Positions 229-314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199060. 7 interactions.
STRINGi10090.ENSMUSP00000079693.

PTM databases

PhosphoSiteiQ60925.

Proteomic databases

PRIDEiQ60925.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080885; ENSMUSP00000079693; ENSMUSG00000059824.
GeneIDi13170.
KEGGimmu:13170.
UCSCiuc009gws.2. mouse.

Organism-specific databases

CTDi1628.
MGIiMGI:94866. Dbp.

Phylogenomic databases

eggNOGiNOG238019.
GeneTreeiENSGT00440000039070.
HOGENOMiHOG000234383.
HOVERGENiHBG004983.
InParanoidiQ60925.
KOiK09056.
OMAiKEPTSCL.
OrthoDBiEOG712TZJ.
PhylomeDBiQ60925.
TreeFamiTF315869.

Enzyme and pathway databases

ReactomeiREACT_115781. Bmal1:Clock,Npas2 activates circadian gene expression.
REACT_344843. BMAL1:CLOCK,NPAS2 activates circadian gene expression.

Miscellaneous databases

NextBioi283264.
PROiQ60925.
SOURCEiSearch...

Gene expression databases

BgeeiQ60925.
CleanExiMM_DBP.
ExpressionAtlasiQ60925. baseline and differential.
GenevisibleiQ60925. MM.

Family and domain databases

InterProiIPR004827. bZIP.
IPR029830. DBP.
[Graphical view]
PANTHERiPTHR11988:SF7. PTHR11988:SF7. 1 hit.
PfamiPF07716. bZIP_2. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Lee Y.H., Oguchi H., Gonzalez F.J.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  3. "The two PAR leucine zipper proteins, TEF and DBP, display similar circadian and tissue-specific expression, but have different target promoter preferences."
    Fonjallaz P., Ossipow V., Wanner G., Schibler U.
    EMBO J. 15:351-362(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CIRCADIAN INDUCTION.
  4. "The loss of circadian PAR bZip transcription factors results in epilepsy."
    Gachon F., Fonjallaz P., Damiola F., Gos P., Kodama T., Zakany J., Duboule D., Petit B., Tafti M., Schibler U.
    Genes Dev. 18:1397-1412(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INVOLVEMENT IN EPILEPSY.

Entry informationi

Entry nameiDBP_MOUSE
AccessioniPrimary (citable) accession number: Q60925
Secondary accession number(s): Q8VCX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 10, 2004
Last modified: June 24, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Mice deficient for all three PAR bZIP proteins (DBP, HLF and TEF) display a dramatically shortened life span and are highly susceptible to generalized spontaneous and audiogenic epilepsies (due for example to the noise of a vacuum cleaner) that are frequently lethal. The down-regulation of pyridoxal kinase (Pdxk) expression in these mice may participate in this seizure phenotype.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.