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Protein

Epidermal growth factor receptor substrate 15-like 1

Gene

Eps15l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2 (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi172 – 183PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-182971. EGFR downregulation.
R-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Epidermal growth factor receptor substrate 15-like 1
Alternative name(s):
Epidermal growth factor receptor pathway substrate 15-related sequence
Short name:
Eps15-rs
Eps15-related protein
Short name:
Eps15R
Gene namesi
Name:Eps15l1
Synonyms:Eps15-rs, Eps15R
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:104582. Eps15l1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: MGI
  • clathrin coat of coated pit Source: MGI
  • membrane Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Coated pit, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001461192 – 907Epidermal growth factor receptor substrate 15-like 1Add BLAST906

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei74PhosphotyrosineCombined sources1
Modified residuei107PhosphoserineCombined sources1
Modified residuei108PhosphoserineCombined sources1
Modified residuei229PhosphoserineBy similarity1
Modified residuei244PhosphoserineCombined sources1
Modified residuei253PhosphoserineCombined sources1
Modified residuei255PhosphoserineCombined sources1
Modified residuei259PhosphoserineBy similarity1
Modified residuei360PhosphoserineCombined sources1
Modified residuei364PhosphothreonineCombined sources1
Modified residuei369PhosphoserineBy similarity1
Modified residuei375PhosphoserineBy similarity1
Modified residuei558PhosphoserineBy similarity1
Modified residuei562PhosphotyrosineCombined sources1
Modified residuei610PhosphoserineCombined sources1
Modified residuei664PhosphoserineBy similarity1
Modified residuei670PhosphoserineBy similarity1
Modified residuei695PhosphoserineBy similarity1
Modified residuei715PhosphoserineBy similarity1
Modified residuei732PhosphoserineBy similarity1
Modified residuei791PhosphoserineBy similarity1
Modified residuei795PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues by EGFR.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ60902.
PeptideAtlasiQ60902.
PRIDEiQ60902.

PTM databases

iPTMnetiQ60902.
PhosphoSitePlusiQ60902.

Expressioni

Gene expression databases

BgeeiENSMUSG00000006276.
GenevisibleiQ60902. MM.

Interactioni

Subunit structurei

Interacts with EPS15, AGFG1/HRB and AGFG2/HRBL. Associates with the clathrin-associated adapter protein complex 2 (AP-2). Interacts with FCHO1 (By similarity). Interacts with FCHO2. Interacts (via EH domains) with DAB2. Interacts with UBQLN1 (via ubiquitin-like domain) (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Dab2P980782EBI-443931,EBI-1391846
Eps15P425672EBI-443931,EBI-443923

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199490. 9 interactors.
IntActiQ60902. 7 interactors.
MINTiMINT-234466.
STRINGi10090.ENSMUSP00000129739.

Structurei

3D structure databases

ProteinModelPortaliQ60902.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 104EH 1PROSITE-ProRule annotationAdd BLAST90
Domaini127 – 215EH 2PROSITE-ProRule annotationAdd BLAST89
Domaini159 – 194EF-handPROSITE-ProRule annotationAdd BLAST36
Domaini273 – 363EH 3PROSITE-ProRule annotationAdd BLAST91
Domaini863 – 882UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini889 – 907UIM 2PROSITE-ProRule annotationAdd BLAST19

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 368Interaction with DAB21 PublicationAdd BLAST354

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili384 – 551Sequence analysisAdd BLAST168

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi748 – 848Pro-richAdd BLAST101

Sequence similaritiesi

Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 3 EH domains.PROSITE-ProRule annotation
Contains 2 UIM (ubiquitin-interacting motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0998. Eukaryota.
ENOG410XTDR. LUCA.
GeneTreeiENSGT00760000118985.
HOGENOMiHOG000004804.
HOVERGENiHBG005591.
InParanoidiQ60902.
KOiK12472.
OMAiQKNSALY.
OrthoDBiEOG091G01RG.
TreeFamiTF324293.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EH_dom.
IPR003903. UIM_dom.
[Graphical view]
PfamiPF12763. EF-hand_4. 3 hits.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
SM00027. EH. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 3 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 4 hits.
PS50031. EH. 3 hits.
PS50330. UIM. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q60902-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPLVPLSQ QIPGGNPLYE SYYKQVDPAY TGRVGASEAA LFLKKSGLSD
60 70 80 90 100
IILGKIWDLA DPEGKGFLDK QGFYVALRLV ACAQSGHEVT LSSLSLTMPP
110 120 130 140 150
PKFHDTSSPL MATQSSAETH WAVRVEEKAK FDGIFESLLP VNGLLSGDKV
160 170 180 190 200
KPVLMNSKLP LDVLGRVWDL SDIDKDGHLD RDEFAVAMHL VYRALEKEPV
210 220 230 240 250
PSILPPPLIP PSKRKKTVFA GAVPVLPASP PPKDSLRSTP SHGSVSSLNS
260 270 280 290 300
TGSLSPKHSV KQPPVAWVVP VADKMRFDEI FLKTDLDLDG YVSGQEVKEI
310 320 330 340 350
FMHSGLTQNL LAHIWALADT RQTGKLSKEQ FALAMYFIQQ KVSKGIDPPQ
360 370 380 390 400
VLSPDMVPPS ERGTPIPDSS STLASGEFTG VKELDDISQE IAQLQREKYS
410 420 430 440 450
LEQDIREKEE AIRQKTSEVQ ELQNDLDRET SSLQELEAQK QDAQDRLDEM
460 470 480 490 500
DQQKAKLRDM LSDVRQKCQD ETQTISSLKT QIQSQESDLK SQEDDLNRAK
510 520 530 540 550
SELNRLQQEE TQLEQSIQAG RAQLETILRS LKCTQDDINQ ARSKLSQLQE
560 570 580 590 600
SHLEAHRSLE QYDQVPDGVS GTSLPDLATL NEGILLAERG GFGAMDDPFK
610 620 630 640 650
NKALLFSNNS QELHPDPFQA EDPFKSDPFK GADPFKGDPF QSDPFSEQQT
660 670 680 690 700
AATDPFGGDP FKESDPFHSS SSDDFFKKQT KNDPFTSDPF TKNPSLPSKL
710 720 730 740 750
DPFESSDPFS SSSISSKGSD PFGTLDPFGS SSFSSAEGFA DFSQMSKPPP
760 770 780 790 800
SGPFSSSLGG TGFSDDPFKS KQDTPALPPK KPAPPRPKPP SGQSTPVSQL
810 820 830 840 850
GSSDFPESPD PFQPLGADSG DPFQNKKGFG DPFSGKDPFA PSSSAKPPKT
860 870 880 890 900
SSSGFADFTS FGNEEQQLAW AKRESEKAEQ ERLARLRRQE QEDLELAIAL

SKADMPA
Length:907
Mass (Da):99,309
Last modified:July 27, 2011 - v3
Checksum:iB5AF1A89B445E8A7
GO
Isoform 2 (identifier: Q60902-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     794-819: STPVSQLGSSDFPESPDPFQPLGADS → YASSSRGTRRWGQGGGHRAPPLSSPE
     820-907: Missing.

Show »
Length:819
Mass (Da):89,728
Checksum:iD46667729E593945
GO
Isoform 3 (identifier: Q60902-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     596-599: DDPF → VKVE
     600-819: Missing.

Show »
Length:687
Mass (Da):75,877
Checksum:iF30E20D19F47A4DA
GO
Isoform 4 (identifier: Q60902-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     565-595: Missing.
     748-860: Missing.

Note: No experimental confirmation available.
Show »
Length:763
Mass (Da):84,752
Checksum:i1739157C3A8045B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14G → S in AAA87202 (PubMed:7568168).Curated1
Sequence conflicti14G → S in AAH15259 (PubMed:15489334).Curated1
Sequence conflicti29A → P in AAA87202 (PubMed:7568168).Curated1
Sequence conflicti170L → V in BAE31350 (PubMed:16141072).Curated1
Sequence conflicti413R → K in AAA87202 (PubMed:7568168).Curated1
Sequence conflicti413R → K in AAH15259 (PubMed:15489334).Curated1
Sequence conflicti726D → H in BAC29523 (PubMed:16141072).Curated1
Sequence conflicti821D → E in AAA87202 (PubMed:7568168).Curated1
Sequence conflicti850T → A in AAA87202 (PubMed:7568168).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022638565 – 595Missing in isoform 4. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_010681596 – 599DDPF → VKVE in isoform 3. 2 Publications4
Alternative sequenceiVSP_010682600 – 819Missing in isoform 3. 2 PublicationsAdd BLAST220
Alternative sequenceiVSP_022639748 – 860Missing in isoform 4. 1 PublicationAdd BLAST113
Alternative sequenceiVSP_010683794 – 819STPVS…LGADS → YASSSRGTRRWGQGGGHRAP PLSSPE in isoform 2. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_010684820 – 907Missing in isoform 2. 1 PublicationAdd BLAST88

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29156 mRNA. Translation: AAA87202.1.
AK036662 mRNA. Translation: BAC29523.1.
AK036728 mRNA. Translation: BAC29554.1.
AK146781 mRNA. Translation: BAE27427.1.
AK152602 mRNA. Translation: BAE31350.1.
CH466525 Genomic DNA. Translation: EDL10796.1.
BC015259 mRNA. Translation: AAH15259.1.
CCDSiCCDS52597.1. [Q60902-1]
RefSeqiNP_001116304.1. NM_001122832.1.
NP_001276788.1. NM_001289859.1. [Q60902-4]
NP_031970.2. NM_007944.3. [Q60902-1]
UniGeneiMm.288894.

Genome annotation databases

EnsembliENSMUST00000163643; ENSMUSP00000129739; ENSMUSG00000006276. [Q60902-1]
ENSMUST00000212121; ENSMUSP00000148468; ENSMUSG00000006276. [Q60902-4]
GeneIDi13859.
KEGGimmu:13859.
UCSCiuc009mfr.3. mouse. [Q60902-1]
uc009mfs.3. mouse. [Q60902-4]
uc009mft.2. mouse. [Q60902-2]
uc009mfu.2. mouse. [Q60902-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29156 mRNA. Translation: AAA87202.1.
AK036662 mRNA. Translation: BAC29523.1.
AK036728 mRNA. Translation: BAC29554.1.
AK146781 mRNA. Translation: BAE27427.1.
AK152602 mRNA. Translation: BAE31350.1.
CH466525 Genomic DNA. Translation: EDL10796.1.
BC015259 mRNA. Translation: AAH15259.1.
CCDSiCCDS52597.1. [Q60902-1]
RefSeqiNP_001116304.1. NM_001122832.1.
NP_001276788.1. NM_001289859.1. [Q60902-4]
NP_031970.2. NM_007944.3. [Q60902-1]
UniGeneiMm.288894.

3D structure databases

ProteinModelPortaliQ60902.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199490. 9 interactors.
IntActiQ60902. 7 interactors.
MINTiMINT-234466.
STRINGi10090.ENSMUSP00000129739.

PTM databases

iPTMnetiQ60902.
PhosphoSitePlusiQ60902.

Proteomic databases

PaxDbiQ60902.
PeptideAtlasiQ60902.
PRIDEiQ60902.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000163643; ENSMUSP00000129739; ENSMUSG00000006276. [Q60902-1]
ENSMUST00000212121; ENSMUSP00000148468; ENSMUSG00000006276. [Q60902-4]
GeneIDi13859.
KEGGimmu:13859.
UCSCiuc009mfr.3. mouse. [Q60902-1]
uc009mfs.3. mouse. [Q60902-4]
uc009mft.2. mouse. [Q60902-2]
uc009mfu.2. mouse. [Q60902-3]

Organism-specific databases

CTDi58513.
MGIiMGI:104582. Eps15l1.

Phylogenomic databases

eggNOGiKOG0998. Eukaryota.
ENOG410XTDR. LUCA.
GeneTreeiENSGT00760000118985.
HOGENOMiHOG000004804.
HOVERGENiHBG005591.
InParanoidiQ60902.
KOiK12472.
OMAiQKNSALY.
OrthoDBiEOG091G01RG.
TreeFamiTF324293.

Enzyme and pathway databases

ReactomeiR-MMU-182971. EGFR downregulation.
R-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

ChiTaRSiEps15l1. mouse.
PROiQ60902.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006276.
GenevisibleiQ60902. MM.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EH_dom.
IPR003903. UIM_dom.
[Graphical view]
PfamiPF12763. EF-hand_4. 3 hits.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
SM00027. EH. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 3 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 4 hits.
PS50031. EH. 3 hits.
PS50330. UIM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEP15R_MOUSE
AccessioniPrimary (citable) accession number: Q60902
Secondary accession number(s): Q3U7L9
, Q3UIS9, Q8CB60, Q8CB70, Q91WH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 158 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.